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Grundalizer
Stranger

Registered: 03/15/15
Posts: 18
Last seen: 6 years, 11 months
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Re: Need some advice [Re: spore-ty]
#23836633 - 11/15/16 07:25 PM (7 years, 2 months ago) |
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I can get you a case of 500 gamma-irradiated sterile petri dishes in sleeves of 20 for $83.
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A.RichardTrickle
Feel like a Stranger

Registered: 11/04/16
Posts: 808
Loc: Silver Mine Peggy Sue
Last seen: 7 years, 1 month
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Quote:
Grundalizer said: I can get you a case of 500 gamma-irradiated sterile petri dishes in sleeves of 20 for $83.
Someone want to split it with me?
-------------------- "When eating shit, it is best not to nibble. Bite, Chew. Swallow. Repeat." "If you're making love to your old lady, someone else is fucking her" "Douchebags are children who never grew up, like Sheeklette, we should pity them." [quote]Niffla said: [quote]A.RichardTrickle said: Dick[/quote] http://www.youtube.com/v/kbwNUOUy-3c[/quote]
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jellyfish


Registered: 10/02/05
Posts: 7,457
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Those petri dishes sound tempting.
My agar jars before germination:

Growth from my 3 year old spore print. Can't tell if it's cubensis or cobweb mould or something. The bit of the left that looks a bit rhizomorphic was removed yesterday and transferred to fresh agar. What do you guys think? I took pictures of the growth on my mexicana and tampanensis plates but the lighting was awful and they didn't work out. I didn't know how to choose what parts of the agar to transfer. Do you look for rhizomorphic growth like you do with cubes?

My flowhood is the coolest thing I've ever built myself
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enlightenment
alchemist


Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 8 months, 22 days
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Re: Need some advice [Re: jellyfish]
#23840509 - 11/17/16 12:57 AM (7 years, 2 months ago) |
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Looks good  If you have a flow hood i highly recommend to use petri dishes. They need less space and it is easier to take a look at the growth. They can not be pre poured but with a flow hood that shouldn't be an issue.

A disadvantage is that petri dishes can not be reused unless you buy glass petri dishes. I had glass dishes once but sold them because I hate to clean them.
Edited by enlightenment (11/17/16 01:03 AM)
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jellyfish


Registered: 10/02/05
Posts: 7,457
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See I have always done things a little more simply, like spore syringes or taking flesh from a healthy mushroom. But now that I'm trying to go from spore prints, and contams are inevitable, I'm really seeing how crappy jars are. Ideally I should be able to glance at my agar and immediately see the whole 2D picture of what's going on. I should be able to spot contams right away. Also I have no idea what I'm doing with the sclerotia forming species so it would be a lot easier for me to show you all pictures and get feedback. I'm going to have to buy some petri dishes, I just don't like disposable stuff, I want glass ones I can reuse.
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enlightenment
alchemist


Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 8 months, 22 days
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Re: Need some advice [Re: jellyfish]
#23840558 - 11/17/16 01:42 AM (7 years, 2 months ago) |
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Quote:
I just don't like disposable stuff, I want glass ones I can reuse.
Good attitude. 
If you don't want to do a lot of species at the same time, using glass dishes is a great idea. Good luck
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 14 hours, 37 minutes
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Re: Need some advice [Re: jellyfish]
#23840636 - 11/17/16 03:22 AM (7 years, 2 months ago) |
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-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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jellyfish


Registered: 10/02/05
Posts: 7,457
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Hey guys. one of my new agar plates from a trasfer I did (cubensis) is showing amazing rhizomorphic growth. Only issue is a bit farther away from the agar wedge is a white spot that doesn't look rhizomorphic. Now, it could be that I was sloppy when I added the agar and a tiny piece broke off, but I'm afraid it's a contam. I want to transfer the super nice growth asap to a new agar plate, but I won't have time until at least tomorrow night, possibly even a few nights. I decided to put it in a ziploc bag and put it in the fridge to slow down the eventual collision between my nice rhizomorphhic splendor and this possible white mould. Bad idea? I'm going to try to make new agar plates tomorrow but honestly I have so many people I live with who are always cooking it's rare for me to get a stove top to do my stuff on.
Hope the fridge wasn't a dumb idea. I put it in a clean ziploc just because I know fridges are notorious for contams.
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A.RichardTrickle
Feel like a Stranger

Registered: 11/04/16
Posts: 808
Loc: Silver Mine Peggy Sue
Last seen: 7 years, 1 month
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Re: Need some advice [Re: jellyfish]
#23852740 - 11/20/16 09:14 PM (7 years, 2 months ago) |
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Pic?
-------------------- "When eating shit, it is best not to nibble. Bite, Chew. Swallow. Repeat." "If you're making love to your old lady, someone else is fucking her" "Douchebags are children who never grew up, like Sheeklette, we should pity them." [quote]Niffla said: [quote]A.RichardTrickle said: Dick[/quote] http://www.youtube.com/v/kbwNUOUy-3c[/quote]
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jellyfish


Registered: 10/02/05
Posts: 7,457
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Can't get a nice pic through the jar but on one side of the plate there's an agar wedge with beautiful rhizomorphic growth spreading out from all sides and on the other side is a bright white spec I'm afraid is a contam. If I don't have time to make agar tomorrow then transfer has to wait till next Monday cause I'm too busy. Was fridge a bad idea?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Need some advice [Re: jellyfish]
#23852923 - 11/20/16 10:56 PM (7 years, 2 months ago) |
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The fridge should be fine. It will slow everything right down.
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jellyfish


Registered: 10/02/05
Posts: 7,457
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So I seem to have isolated some rhizomorphic growth from my cubensis spores. The galindoi and tampanensis spores (a year old) didn't germinate. I have isolated some of what looked like healthy growth from each of my 4 tampanensis "plates" to 2 new plates each. Mycellium still seems really fluffy. Until I have a lamp by my flowhood I will not be able to provide good pics, but I think with one more transfer I will have a really nice cube culture. Can anyone direct me to pics of what sclerotia forming species are supposed to look like on agar? I am so used to cubes.
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PirateSwazey


Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Re: Need some advice [Re: jellyfish]
#23877879 - 11/29/16 10:39 AM (7 years, 2 months ago) |
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jellyfish


Registered: 10/02/05
Posts: 7,457
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Thanks a lot that's really helpful
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jellyfish


Registered: 10/02/05
Posts: 7,457
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So I will call the spores to agar plates gen 1. For cubensis (golden teacher spores I took myself 3 over years ago) gen 1 was pretty dirty. Some gen 2 plates are contam'd but a couple have nothing by mycorhizzal goodness. Because it's christmas baking season I'm not sure when I'll be able to PC some rye so I'm thinking of putting the best cultures in ziploc bags in the fridge and occasionally taking them out to open them to let them breathe as I know even with tyvek, fridges are full of mould spores. Speaking of tyvek the 2 stores that sold tyvek painting suits are gone from my area so I need to find a new source. One of the best looking plate actually fully colonized and then fruited a sad little shroom. For gen 3, I took all the best rhizomorphic growth, except for one plate where I just extracted the fruting body and put him on directly. Looking back that may have been done, should have cut the cap off so I don't have spores released cause I'd like some type of isolate. Every other gen 3 plate came from a clean gen 2 plate except the one I had put in the firdge that had brilliant rhizomorphic growth but contam on the other side of the plate, which I sliced out last night for a gen 3 culture. If my gen 3 cultures wen't well then I have about 3 fully colonized rhizomorphic gen 2 plates, (one of which fruited), and 5 gen 3 plates I tried my best to isolate from the nicest growth. Like I said, I don't want my gen 2 plates fruiting again so is fridge the way to go until I have time to get some rye going?
Tampanenesis and galindoi prints I bought from a respected vendor did nothing, I have growth on the mexicana plates. Gen 1 looked nasty. Gen 2 looked a lot less nasty but still some obvious contam. Did my best to isolate the mycellium from gen 3 that looked like the pictures posted above. I am wondering, like I said it will be a while until I have rye going. The gen 2 plates that are clean, I may refrigerate so they don't end up fruiting in the jar. I made the agar thick so that they won't dry out too quick but they are fully coloznized with beautiful perfect looking growth. The gen 3 plates I made still have a week or two to colonize. My question is, should I leave the mexicana plates (gen 3 which is hopefully clean, and gen 2, a couple of which don't look contamed, and wait until I see sclerotia form? Then transfer those to rye in order to be sure I have the right stuff? This will buy me some time until I can get rye going. The mycellium bruises blue when I cut it which is a plus. Still don't have a scalpel so I pressure cook my whole nail clippers in a tyvek lidded jar and use the little knife attached to them and flame it every time over my alcohol lamp.
Absolutely loving this flow hood. My basement is beyond dusty. Food moulds so quick, we even had a mould issue on the walls at one point. Yet the majority of my cultures are clean. Wish lighting in my basement was better so I could show you the mexicana agar cultures but the cubensis are picture perfect atm. 3 year old spore print, first spore print I've ever taken. Pretty awesome.
edit: Don't want to double post but to sum up what I'm asking:
1) should my fully colonized plates cubensis plates with beautiful rhizomorphic growth be okay in the fridge in ziploc for a bit (couple weeks) before I am ready for rye? I will take them out periodically and open them to let fresh air in. I don't want them fruiting on agar.
2 a) For the mexicana plates that seem okay, should I wait to see sclerotia before moving to rye
2 b) Or should I wait to see sclerotia and then transfer that to fresh agar, as I know it's prone to truffle formation? Thanks.
All these are half pint jars with agar and tyvek lids I made almost a decade ago. I need new tyvek but haven't had the time to research who still sells those suits. I absolutely love tyvek + flow hood. Makes this hobby a breeze. I can open sterile agar in front of my fan in the dustiest basement for 5 minutes and get no contams. I just got a scalpel too which I can PC before use and then flame with my make shift alcohol lamp.
Next step is to grow something legal. Only growing the cubes to get prints as my prints were 3 years old and wanted to revive them before it was too late. Also gives me something to trade with you guys.
Edited by jellyfish (12/07/16 11:36 AM)
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jellyfish


Registered: 10/02/05
Posts: 7,457
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So pretty much every one of my cultures is nothing but mould. Dunno what I did the last set of transfers. Bleached the area, worked in front of laminar flow hood, flamed tools in between uses. Even pressure cooked the tools before use. But now I have to start from scratch. There's a chance one or two of my nice cubensis cultures are clean but all my mexicana are nothing but mould. I've never had this happen since getting my flow hood. So sad.
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jellyfish


Registered: 10/02/05
Posts: 7,457
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Alright I'm sick of this tyvek jar thing. How do these petri dishes come? Are they all individually sealed? I have a flow hood but even still I'm worried about contam. Also all my golden teacher jars show rhizomorphic growth I'm just stalling till holidays are over since I can't do any rye work while the kitchen is so busy.
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jellyfish


Registered: 10/02/05
Posts: 7,457
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So I am still unsure if I am growing mexicana or some sort of wispy white mould since I've never worked with it before. I did pluck this out of one of my agar jars. A piece of scleortia perhaps?
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jellyfish


Registered: 10/02/05
Posts: 7,457
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Re: Need some advice [Re: jellyfish]
#24054921 - 01/31/17 03:39 PM (6 years, 11 months ago) |
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Just an update. Managed to revive 3 year old GT spore print I took myself as well as a mexicana spore print I bought years ago. The other sclerotia forming spore prints I bought at the same time gave me nothing but mould and isolating a clean culture from the mexicana was a bit difficult. Wasn't used to the appearance of the mycellium and had a lot of mould initially but finally cleaned it up.

The leftmost 3 jars are mexicana, the last 2 are golden teacher. My agar cultures are drying out and the GT agar jars are all fruiting off the agar. I am assuming I should just ditch them, clean them and then use these rye jars to inoculate more rye and also start more agar cultures. I have read different things about whether or not to shake mexicana rye jars. Also, I am planning on using one of the three mexicana jars for G2G to start more rye. This should be fine once the jar is fully colonized correct?
Edited by jellyfish (01/31/17 03:45 PM)
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Mikeify
Wildin Foo



Registered: 01/30/17 
Posts: 288
Loc: USA
Last seen: 3 years, 2 months
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Re: Need some advice [Re: jellyfish]
#24055302 - 01/31/17 06:09 PM (6 years, 11 months ago) |
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Im also looking forward to growing GT's but are they prone to bacteria?
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