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ManchildNoob
Paranoid Inpatient Noob



Registered: 11/11/16
Posts: 28
Last seen: 5 years, 9 months
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Koh Samui Cubensis PF Tek, LC first attempt and Log
#23824015 - 11/11/16 08:00 PM (7 years, 2 months ago) |
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Hi all, noob here. First post, first grow attempt, I would love some input from the vast amounts of knowledge the members of this forum posses while I log my grows. I used the PF Tek, 2:1:1 of verm, BRF, water but all the jars look different after an hour and a half of pressure cooking. I inoculated anyway using a sterile syringe sucking up boot leg distilled water (small bowl sitting in pot of tap water with lid on upside down allowing steam to condensate and drip into the bowl) after that cooled I created a spore syringe in my sterile glovebox, inoculating each jar with flaming between. The have been incubating for about a week now at room temperatures between 17C - 25C and no mycelium is visible yet. I took sterility very seriously and I understand the jars aren't optimum however the cake will slide out even with jars with shoulders as below the shoulders it is tapered and above is the layer of dry verm. Damn my impatience! It is scary ordering spores down here! Thanks everyone for helping out a noob! Let me know if you want me to continue updating this log.

 These are the jars a few days after inoculation, see how the smaller jars are lighter than the bigger jars, all the same mixture and method as it is the same batch.
Edited by ManchildNoob (11/17/16 04:56 PM)
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ManchildNoob
Paranoid Inpatient Noob



Registered: 11/11/16
Posts: 28
Last seen: 5 years, 9 months
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Re: Koh Samui Cubensis PF Tek, LC first attempt and Log [Re: ManchildNoob]
#23842703 - 11/17/16 05:28 PM (7 years, 2 months ago) |
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A week later 11/18/16:
6-7 days later and we have mycelium! (I hope) Still incubating at the same temperatures, the whiter coloured BRF jar is colonizing faster in my opinion, however it may also be an illusion as the other jars are bigger, wider and deeper. I have a concern, that the smaller jars have no hole in the lid and they are sealed, will this be okay until birthing? I'm worried about opening the lid for air exchange due to contams. Other jars have both layer of dry verm, hole in lid and covered with aluminium.
 
Liquid Cultures! I had half, to a quarter, of the tiny spore print left so I decided to scrape it into two pressure cooked water/honey mixtures and seal it with a lid. One to use after my grow, or as backup if this grow goes wrong, the other as an extended storage of tissue for and to hopefully have fast colonization in future attempts. Hopefully clarity is okay Here are the liquid cultures I inoculated 3 days after the cakes. It's been about 5 days now.

Possible Contam!?! Oh no! I'm pretty sure one of my jars is contaminated, though I have to say this jar was a bit of an experiment. There is lots of coffee grinds available from my family and friends. I mixed some with my BRF and Verm and added a layer to the top of this jar under the dry verm. Also pressure cooked with the rest. Just to see if any differences occurred, just like this contam  Take a look:

Thanks for checking in again, I welcome all feedback both on technique and how to post a log!
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ManchildNoob
Paranoid Inpatient Noob



Registered: 11/11/16
Posts: 28
Last seen: 5 years, 9 months
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Re: Koh Samui Cubensis PF Tek, LC first attempt and Log [Re: ManchildNoob]
#24599182 - 09/02/17 07:47 PM (6 years, 4 months ago) |
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Just an update
Unfortunately I have no pictures but these cakes took months to fully colonize, I still have the liquid culture and plan on seeing if it is still alive by innoculating more. The cakes were soaked and placed in shot gun fruiting chamber and fuited very nicely, gave about four flushes (even the tiny jars) and were potent enough for CEV and all day effects. though after this the cakes were spent and harvested moulds
Edited by ManchildNoob (09/02/17 07:48 PM)
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OneBadMan
Stranger Danger!!



Registered: 02/25/10
Posts: 218
Loc: Mushroom City
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Re: Koh Samui Cubensis PF Tek, LC first attempt and Log [Re: ManchildNoob]
#24599200 - 09/02/17 07:55 PM (6 years, 4 months ago) |
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Ouch don't be discouraged.. At least you got few flushes .. Did you clone or at least print some more solid genetics?Lc are a major failure.. Unless you are advanced in AGAR... But none the less you learn and live to grow some more .. Well done boss man..
--------------------
Everything I Say & show is Made up, Pic Download from Internet. I like to Make Everyone think I'm cool, i would never Break Any Law's!
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Mirlin
Mkultra



Registered: 08/05/17
Posts: 199
Last seen: 6 years, 4 months
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Re: Koh Samui Cubensis PF Tek, LC first attempt and Log [Re: OneBadMan]
#24599238 - 09/02/17 08:17 PM (6 years, 4 months ago) |
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Dont use liquid cultures.
If you have a clean spore syringe you can expand out on it just by adding 2.5cc's to a bottle of spring water and shaking or by refilling the syringe with spring water.
Same results just different ways.
A dark spore syringe can be reused at least 10x before its completely spent of spores.
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ManchildNoob
Paranoid Inpatient Noob



Registered: 11/11/16
Posts: 28
Last seen: 5 years, 9 months
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Re: Koh Samui Cubensis PF Tek, LC first attempt and Log [Re: Mirlin]
#24671146 - 09/29/17 07:12 PM (6 years, 3 months ago) |
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Another short update
I placed a drop of the aprox. 1 year old LC onto agar and it is still alive and spreading with no signs of contamination. Is it worth my while to isolate from this random drop, due to the mycelium being submerged in a bit of water there were multiple points of colonization on the agar, but again no contams. BTW the LC was not kept in a fridge, just in room temp.
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: Koh Samui Cubensis PF Tek, LC first attempt and Log [Re: ManchildNoob]
#24671370 - 09/29/17 09:00 PM (6 years, 3 months ago) |
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There is no good reason to. You have no clue what you are isolating.
-------------------- JOIN THE POW WOW
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in_dot
Shake N' Bake



Registered: 04/03/17
Posts: 719
Loc: East Coast
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Re: Koh Samui Cubensis PF Tek, LC first attempt and Log [Re: TheMadHatter420]
#24671516 - 09/29/17 09:40 PM (6 years, 3 months ago) |
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Trying to isolate straight from MS genetics would take soooooooo many attempts. I tried it this summer while learning agar and I got to around transfer 10 I think before I gave up. Sometimes you have to take 3-4 transfers of MS just to begin to see sectoring.
-------------------- Loneliness is a term used by those that aren't comfortable with themselves Once one conquers loneliness, others are never needed to find happiness
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ManchildNoob
Paranoid Inpatient Noob



Registered: 11/11/16
Posts: 28
Last seen: 5 years, 9 months
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Re: Koh Samui Cubensis PF Tek, LC first attempt and Log [Re: in_dot]
#24671920 - 09/30/17 06:22 AM (6 years, 3 months ago) |
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So isolating from an LC is different to isolating from something else like a cake or WBS? I would have no clue what I was isolating from unless it was a fruit body clone which still would have an air of mystery right? What would be the best suggestion with what I have to play with at the moment? I currently have agar, wbs, and some brown rice flour cakes all from the same MS solution. I always though unless isolating from a fruit body that the end result would always be a mystery until fruiting time anyway? Maybe I still need a lot of education.. I am reaching out for some friendly and helpful advice
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Cookies

Registered: 02/15/04
Posts: 1,492
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Re: Koh Samui Cubensis PF Tek, LC first attempt and Log [Re: ManchildNoob]
#24671951 - 09/30/17 06:54 AM (6 years, 3 months ago) |
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Nice.
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in_dot
Shake N' Bake



Registered: 04/03/17
Posts: 719
Loc: East Coast
Last seen: 3 years, 11 months
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Re: Koh Samui Cubensis PF Tek, LC first attempt and Log [Re: ManchildNoob]
#24671984 - 09/30/17 07:37 AM (6 years, 3 months ago) |
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I'm not sure what you mean with isolating from a cake or WBS. Isolating means to transfer genetics from agar dish to agar dish though the process of identifying sectoring on the agar plate, then cutting wedges for transfer from the leading edges.
You are right that if you isolate straight from multispore (MS) genetics you will have no idea what you will get until you grow it out. That's why many (including myself) recommend that you grow out a monotub, or a cake and find some fruits that you like then clone them. At this point you can begin to isolate if you want since you have an idea of the genetics you are working with.
As for what to do right now... Let the cakes colonize, nothing you can do with them now. Also, let the WBS colonize and shake at 30% colonization. Just keep transferring the agar until you get clean growth then use this to either make some liquid inoculant (LI) or just drop the agar wedges in some grain jars.
-------------------- Loneliness is a term used by those that aren't comfortable with themselves Once one conquers loneliness, others are never needed to find happiness
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