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Sirtalis



Registered: 10/05/16
Posts: 409
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Self-healing injection port
#23823173 - 11/11/16 02:21 PM (7 years, 2 months ago) |
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Hello, I am growing for the first time and have been looking all over for info on self-healing injection ports. I have made multiple batches of clean LC from agar and want to step up my lid game for easier colonization. I'm looking to find an easy way to suck up LC.
I purchased one of these:

I've found a lot of threads on self-healing injection ports but I'm mostly seeing this for small gauge needles. I like the design of this tek but I'm not sure that it can "heal" with such a large needle.
https://www.shroomery.org/forums/showflat.php/Number/11487687
Can anyone give me some insight as to wether or not this would work? Thank you.
Edited by Sirtalis (11/11/16 02:29 PM)
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Morel Guy
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Re: Self-healing injection port [Re: Sirtalis]
#23823178 - 11/11/16 02:22 PM (7 years, 2 months ago) |
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Get a tube of silicone.
-------------------- "in sterquiliniis invenitur in stercore invenitur" In filth it will be found in dung it will be found
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,071
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Re: Self-healing injection port [Re: Sirtalis]
#23823181 - 11/11/16 02:24 PM (7 years, 2 months ago) |
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you can use rtv silocone
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enlightenment
alchemist


Registered: 08/09/09
Posts: 1,647
Loc: Europe
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Re: Self-healing injection port [Re: Sirtalis]
#23823184 - 11/11/16 02:25 PM (7 years, 2 months ago) |
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You can use silicone as DIY injection ports. But if you already ordered it may be too late. I have no access to view the link.
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Sirtalis



Registered: 10/05/16
Posts: 409
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Quote:
enlightenment said: You can use silicone as DIY injection ports. But if you already ordered it may be too late. I have no access to view the link. 
I edited the OP with a photo instead of the buy link. It's a big needle used for turkey injections.
Thanks for the link. Looks exactly like what I need. Maybe I'll just make a larger hole for the needle.
Edited by Sirtalis (11/11/16 02:31 PM)
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enlightenment
alchemist


Registered: 08/09/09
Posts: 1,647
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Re: Self-healing injection port [Re: Sirtalis]
#23823202 - 11/11/16 02:32 PM (7 years, 2 months ago) |
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Holy shit Why do you want to use a needle like this? If you have a stir bar inside your LC you don't need a needle like this...


Silicone as an injection port works great.
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Sirtalis



Registered: 10/05/16
Posts: 409
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No stir bar unfortunately, but its next on the list. I want a large needle so I can suck up plenty of myc. I see some tube coming off your syringe, I'm not sure how you'd be able to suck up LC without some sort of specialized lid.
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blackout


Registered: 07/16/00
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Last seen: 2 months, 25 days
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Re: Self-healing injection port [Re: Morel Guy]
#23823226 - 11/11/16 02:39 PM (7 years, 2 months ago) |
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That's an interesting needle, I cannot tell how thick it is, but in the image it appears to have a sharpened solid point, and the liquid outlet is not at the end but up the side. If you have a regular needle the opening is at the bottom and if it is large you risk cutting a core of silicone out of the SHIP. With that needle the point should enter it and push the silicone outwards and hopefully pass on through without cutting or snagging silicone with it.
I would be using silicone caulk and not those commercial inserts. I would also use plenty of silicone, I use clear stuff so I can see where the hole is, which is important if you lay it on thick.
When using SHIPs I cover them with foil or plastic bags, these get taken off just before inoculation, most preferably in a SAB. I have been using large long clear plastic bags as SABs lately.
EDIT: the link worked fine for me, its a 40ml syringe if people want an idea of the size. http://www.homedepot.com/p/Charcoal-Companion-Plastic-Marinade-Injector-CC5111/204780026?cm_mmc=Shopping%7cTHD%7cG%7c0%7cG-BASE-PLA-D28I-Grills%7c&gclid=CMPK6MrQodACFRVrfgod4J0MEw&gclsrc=aw.ds
Edited by blackout (11/11/16 02:45 PM)
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enlightenment
alchemist


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Posts: 1,647
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Re: Self-healing injection port [Re: Sirtalis]
#23823233 - 11/11/16 02:43 PM (7 years, 2 months ago) |
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That's just the cap of the needle. I can turn the LC jar 90°. I suck it up with a needle through the silicone port.
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Sirtalis



Registered: 10/05/16
Posts: 409
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So its looking like silicone is the way to go. I'll try it out on a lid and see if the needle is too large for the port to heal itself. Do you guys inject while the needle is warm to allow it to pass through easier? Or will that just burn the silicone.
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enlightenment
alchemist


Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 8 months, 22 days
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Re: Self-healing injection port [Re: Sirtalis]
#23823251 - 11/11/16 02:51 PM (7 years, 2 months ago) |
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I spray the port with disinfectant before I inject. I flame the needle until it is red and let it cool in front of the flow hood. But you can just let it cool a few seconds in the air or SAB. I would not poke it through the port while the needle is still very hot.
//edit
Ah no.. wrong.. If it is a new sterile needle I do not flame it of course... I just flame it if it was already used.
Edited by enlightenment (11/11/16 02:54 PM)
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blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 2 months, 25 days
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I would also question the material, it may not hold up to being sterilized. The outer looks like PP but looks like a white plunger, not sure about it. If it was mine I would heat it to about 80 or 90C and see if it gets in soft at all, if it did I would keep it for marinating, rather than boiling it up first and potentially ruining it fully.
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enlightenment
alchemist


Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 8 months, 22 days
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Re: Self-healing injection port [Re: blackout]
#23823271 - 11/11/16 02:57 PM (7 years, 2 months ago) |
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Keeping it for marinating is a good idea blackout.
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blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 2 months, 25 days
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Quote:
Sirtalis said: I'll try it out on a lid and see if the needle is too large for the port to heal itself.
good idea, and do a few practise runs with it, if you are using a SAB it is best to practice inside to iron out any awkwardness and things you might change in future.
Quote:
Sirtalis said: Do you guys inject while the needle is warm to allow it to pass through easier? Or will that just burn the silicone.
It can damage the silicone, a large needle will also hold more heat if you have flamed it and so cause even more damage, just like an aluminium baking tray would burn your fingers more than aluminium foil at the same temperature -it holds more heat.
Your syringe has no cap. I would wrap it in foil before sterilizing, personally I would make a cap for it from thin silicone tube, but you may not have that handy. You do not really need the cap anyway, I suck water into the syringes and sterilize them and let them cool, you can then flame the needle well and squirt out the cold sterile water to cool it.
If you suck up LC you can leave the syringe with the needle facing upwards for a moment, then the myc settles in the bottom a bit, now you can flame the needle and squirt out the clear upper layer to cool the needle before injection. It only takes a tiny amount to cool it. Again you could test this with regular unsterile water before doing real work.
Quote:
enlightenment said: I spray the port with disinfectant before I inject.
I cover the port with foil or a plastic bag so it remains sterile until needed, but if doing multiple injections I would disinfect, sometimes, if its in a SAB and done one after each other I would not bother. You can also have mulitple SHIPs on the same lid, so they could have different foil coverings, and also you are not wearing out the same one over and over.
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
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Re: Noob needle port [Re: Sirtalis]
#23823321 - 11/11/16 03:14 PM (7 years, 2 months ago) |
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-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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NDStepp84
Stellar nuclear waste


Registered: 04/23/15
Posts: 4,956
Last seen: 1 month, 4 days
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That needle looks like the 88 cent maranade injection syringes from Walmart
 , if so that thing is like a 10 gauge or better, I doubt an injection port will heal after being stabbed by that thing. The fact that the end doesn't have a hole but a tapered point may help but still a big fkn needle.
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"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do. -Robert A. Heinlein
Links and teks ND's grow log and discussion Plant thread
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 12 hours, 28 minutes
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Re: Noob needle port [Re: NDStepp84]
#23823534 - 11/11/16 04:39 PM (7 years, 2 months ago) |
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Open the lid with that injection port wounder.
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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