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OfflineCagney
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G2G question
    #23822296 - 11/11/16 08:40 AM (7 years, 2 months ago)

I have several quarts of WBS that is fully colonized but want to switch
to using rye berries. Is it okay to put the colonized WBS in with rye berries. I figure it should work just looking for confirmation before I do it.


--------------------


"The time has come," the Walrus said, "To talk of many things: Of shoes--and ships--and sealing-wax-- Of cabbages--and kings-- And why the sea is boiling hot-- And whether pigs have wings."


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InvisiblebodhisattaMDiscordReddit
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Re: G2G question [Re: Cagney]
    #23822307 - 11/11/16 08:46 AM (7 years, 2 months ago)

Tell me why it wouldn't be ok.


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OfflineThe Mycologist
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Re: G2G question [Re: bodhisatta]
    #23822310 - 11/11/16 08:49 AM (7 years, 2 months ago)

It depends, how did you inoculate the wbs? If with agar then you should be good to go.


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
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OfflineA.RichardTrickle
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Re: G2G question [Re: The Mycologist]
    #23822312 - 11/11/16 08:51 AM (7 years, 2 months ago)

Quote:

The Mycologist said:
It depends, how did you inoculate the wbs? If with agar then you should be good to go.



What difference does this make?  I'm still (always actually) learning. :thumbup:


--------------------
"When eating shit, it is best not to nibble.  Bite, Chew. Swallow. Repeat."
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"Douchebags are children who never grew up, like Sheeklette, we should pity them."
[quote]Niffla said:
[quote]A.RichardTrickle said:
Dick[/quote]
http://www.youtube.com/v/kbwNUOUy-3c[/quote]


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InvisiblebodhisattaMDiscordReddit
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Re: G2G question [Re: The Mycologist]
    #23822317 - 11/11/16 08:54 AM (7 years, 2 months ago)

Quote:

The Mycologist said:
It depends, how did you inoculate the wbs? If with agar then you should be good to go.



No it doesn't depend


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OfflineThe Mycologist
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Re: G2G question [Re: bodhisatta]
    #23822329 - 11/11/16 08:58 AM (7 years, 2 months ago)

Well you wouldn't recommend someone to go bulk with spore syringe as the inoculate right?


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
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Re: G2G question [Re: The Mycologist] * 1
    #23822331 - 11/11/16 08:59 AM (7 years, 2 months ago)

Recommended no, 100% possible yes. Hundreds have done it that way


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InvisibleMad Season
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Re: G2G question [Re: bodhisatta]
    #23822332 - 11/11/16 09:00 AM (7 years, 2 months ago)

If it's clean, go for it.


--------------------
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Offlinetump
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Re: G2G question [Re: Mad Season]
    #23822357 - 11/11/16 09:11 AM (7 years, 2 months ago)

Pics. And go for it. Grain is easy to make more off


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OfflineCagney
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Re: G2G question [Re: tump]
    #23822598 - 11/11/16 10:39 AM (7 years, 2 months ago)

I take it this is 100% colonized, it's solid all the way around for about a week and a half


--------------------


"The time has come," the Walrus said, "To talk of many things: Of shoes--and ships--and sealing-wax-- Of cabbages--and kings-- And why the sea is boiling hot-- And whether pigs have wings."


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Re: G2G question [Re: Cagney]
    #23822608 - 11/11/16 10:42 AM (7 years, 2 months ago)

Those lids are fucked though


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OfflineCagney
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Re: G2G question [Re: bodhisatta]
    #23822621 - 11/11/16 10:50 AM (7 years, 2 months ago)

Why you say that? I have large holes filled with polyfil in them. I just put the coffee filters on top of them because I have several cases of them stacked on top of each other, and I write myself notes on them. But they should be 100% colonized now?


--------------------


"The time has come," the Walrus said, "To talk of many things: Of shoes--and ships--and sealing-wax-- Of cabbages--and kings-- And why the sea is boiling hot-- And whether pigs have wings."


Edited by Cagney (11/11/16 10:52 AM)


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InvisibleComebackKid
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Re: G2G question [Re: Cagney]
    #23822653 - 11/11/16 11:05 AM (7 years, 2 months ago)

Quote:

Cagney said:
I take it this is 100% colonized, it's solid all the way around for about a week and a half




No need for a consolidation period with grains. When they're fully colonized they're ready to spawn to bulk.
Yes you can mix different kinds of spawn jars. You could even go as far as mixing your rye and wbs in the same jar with popcorn before PC. Myc won't discriminate.


--------------------
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is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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Re: G2G question [Re: Cagney]
    #23823168 - 11/11/16 02:18 PM (7 years, 2 months ago)

Quote:

Cagney said:
Is it okay to put the colonized WBS in with rye berries.



yes, I guess your WBS is already a mixture of grains anyway. I have noticed it can take longer for different grains to take off when mixed, I can't recall the term but its that they are not used to the new grains and so have a slight delay getting used to them. I have noticed this also with the exact same grains but at different moisture contents, like adding pretty wet colonised popcorn grain to drier grains of the same popcorn.

To test if the grains are clean I will sometimes shake them up to see if they recolonize in a few days, it is no guarantee though. If they do not colonise again they are most likely contaminated and so would have fucked up all the jars you transferred to.


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OfflineCagney
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Re: G2G question [Re: blackout]
    #23823397 - 11/11/16 03:37 PM (7 years, 2 months ago)

Alright now this shit is getting more complicated than I thought. So I should shake them up and see if they recolonize before I start. Last thing I want to do is put in a lot of work and lose it to contamination. I am also looking at agar and Petri's on eBay to buy at this very moment, for sake of not looking it up what is a good type to start with? I am seeing soy agar, potato agar, seaweed agar, and I figure it's best to go with glass petri's.


--------------------


"The time has come," the Walrus said, "To talk of many things: Of shoes--and ships--and sealing-wax-- Of cabbages--and kings-- And why the sea is boiling hot-- And whether pigs have wings."


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Offlineenlightenment
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Re: G2G question [Re: Cagney]
    #23823413 - 11/11/16 03:42 PM (7 years, 2 months ago)

My favorite is malt extract agar (MEA). Working with glass petris is a PITA.


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OfflineCagney
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Re: G2G question [Re: enlightenment]
    #23823437 - 11/11/16 03:53 PM (7 years, 2 months ago)

Working with glass petri is a PITA.... Do, who, what? PITA?


--------------------


"The time has come," the Walrus said, "To talk of many things: Of shoes--and ships--and sealing-wax-- Of cabbages--and kings-- And why the sea is boiling hot-- And whether pigs have wings."


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OfflineKenetic
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Re: G2G question [Re: Cagney]
    #23823443 - 11/11/16 03:57 PM (7 years, 2 months ago)

I like both mea and plastic petris.  I've only used them so I can't really compare but I can think of some advantages.
I go through a lot and it's nice to not have to add an extra step (sterilizing dishes) when I'm already busy enough as it is.  I could just throw them in with the agar but it's still an extra step I don't want.  Plastic petris I just wipe down the packaging with iso and cut the bottom and slide the plastic off. 
Plastic dishes are also clearer than glass, believe it or not.  Glass is slippery too.  Plastic is cheap and can be recycled. 
Mea is a good well-rounded nutrient agar to start with.  I find it suitable for all my needs since I'm not doing any long-term transfers right now, but if you want to do that pick another mix and switch it up to keep the myc vigorous. 

Whatever you choose, have fun with it.  You probably won't be able to stop yourself anyway lol


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



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Offlineenlightenment
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Re: G2G question [Re: Cagney]
    #23823445 - 11/11/16 03:58 PM (7 years, 2 months ago)

It is a 'pain in the ass' :laugh: You have to clean them every time. You have to sterilize them...

Sterilized polystyrene petri dishes are comfortable to work with. You can not use them multiple times but it is easier to work with them.


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OfflineCagney
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Re: G2G question [Re: enlightenment]
    #23823460 - 11/11/16 04:03 PM (7 years, 2 months ago)

Hey Kenetic what size petri's are those?


--------------------


"The time has come," the Walrus said, "To talk of many things: Of shoes--and ships--and sealing-wax-- Of cabbages--and kings-- And why the sea is boiling hot-- And whether pigs have wings."


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OfflineKenetic
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Re: G2G question [Re: Cagney]
    #23823498 - 11/11/16 04:20 PM (7 years, 2 months ago)

60 mm


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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OfflineCagney
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Re: G2G question [Re: Kenetic]
    #23823559 - 11/11/16 04:52 PM (7 years, 2 months ago)

Okay is there a certain type of Petri dish I need to get. I am looking on e say and there is a stack of 20 plastic 60mm petri's for $4.99, but it says"TC", says not treated. Does that matter?


--------------------


"The time has come," the Walrus said, "To talk of many things: Of shoes--and ships--and sealing-wax-- Of cabbages--and kings-- And why the sea is boiling hot-- And whether pigs have wings."


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OfflineKenetic
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Re: G2G question [Re: Cagney]
    #23823659 - 11/11/16 05:27 PM (7 years, 2 months ago)



--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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Offlineblackout
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Re: G2G question [Re: The Mycologist]
    #23823670 - 11/11/16 05:31 PM (7 years, 2 months ago)

Quote:

Cagney said:
Alright now this shit is getting more complicated than I thought. So I should shake them up and see if they recolonize before I start. Last thing I want to do is put in a lot of work and lose it to contamination.



Shaking them up and waiting a few days longer is not that complicated.

early on you were asked
Quote:

The Mycologist said:
It depends, how did you inoculate the wbs? If with agar then you should be good to go.



this was really asking if you inoculated the WBS with spores direct from a spore syringe, or if you had first put spores on agar and grown them out and confirmed they were clean.

Your subsequent posts would suggest you injected spores, A.K.A. MS, multispore. Many cautiously presume that all spore syringes are contaminated and so strongly recommend you "clean them up" by using agar. You might have been lucky, as you have seemingly used syringes I would strongly recommend at least shaking them and seeing if they recover. If you are a total beginner I would advise casing one of the jars as is, to have more chance of at least growing some mushrooms.

If using agar I would look up the thread on pastyplates. I have glass petris and like them but they are expensive and fragile, you can use plastic alternatives, or use glass food jars.


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Re: G2G question [Re: Cagney]
    #23823714 - 11/11/16 05:44 PM (7 years, 2 months ago)

Quote:

Cagney said:
I am looking on e say and there is a stack of 20 plastic 60mm petri's for $4.99, but it says"TC", says not treated. Does that matter?



Yes, the majority of plastic dishes cannot be heat treated, so if they are not treated you cannot treat them easily. They usually will treat them with radiation or perhaps UV light and not heat like we do. They are usually polystyrene, PS, and cannot hold up to heat. Ones which can are very expensive, but some shallow dip containers can make good susbstitutes.

Can you link to the ones you found on ebay, I expect I could find cheaper. I was able to get 10 55x15mm plates for €1.93 including postage which were sterile and in a sleeve.


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OfflineCagney
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Re: G2G question [Re: blackout]
    #23824161 - 11/11/16 09:18 PM (7 years, 2 months ago)

I was going to spawn 6 quarts of the colonized WBS to bulk, coir/verm. Is that a bad idea?


--------------------


"The time has come," the Walrus said, "To talk of many things: Of shoes--and ships--and sealing-wax-- Of cabbages--and kings-- And why the sea is boiling hot-- And whether pigs have wings."


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OfflineA.RichardTrickle
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Re: G2G question [Re: Cagney]
    #23824425 - 11/11/16 11:06 PM (7 years, 2 months ago)

I am sort of in the same boat as you.
From what I have read people are successful with MS to grain quite frequently.
I think the point is that MOST aspire syringes have some variety of contaminant in them or else are introduced during inoculation.  The jars may look great, however, if bacterial they will infest your bulk sub and weaken the mycelium allowing molds to move in and take a hold.
I have been successful in MS to CVG in the past myself, however, I never get the nice, uniform canopy and rarely make it past second flush with out contams.
If you have the spawn and it looks and smells good you will most likely be fine.  Just putting spores to agar first will bring higher sucsess rates.
Dick


--------------------
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"Douchebags are children who never grew up, like Sheeklette, we should pity them."
[quote]Niffla said:
[quote]A.RichardTrickle said:
Dick[/quote]
http://www.youtube.com/v/kbwNUOUy-3c[/quote]


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OfflineCagney
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Re: G2G question [Re: A.RichardTrickle]
    #23825029 - 11/12/16 06:47 AM (7 years, 2 months ago)

I am fixing to prep my coir this morning, finally got a day off from work. I don't have any gypsum, I planned on just using vermiculite. Does it matter if I have the gypsum or not? Thought I fumbled around and found a Tek once that just used coir & verm.


--------------------


"The time has come," the Walrus said, "To talk of many things: Of shoes--and ships--and sealing-wax-- Of cabbages--and kings-- And why the sea is boiling hot-- And whether pigs have wings."


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Offlinespacechildo
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Re: G2G question [Re: Cagney]
    #23825461 - 11/12/16 10:15 AM (7 years, 2 months ago)

nah gypsum is just an additive its not really needed, just follow the simple bucket tek aka "damion5050's elementary coir tek".

spawning grains to bulk is never a bad idea :wink:


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OfflineCagney
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Re: G2G question [Re: blackout]
    #23844482 - 11/18/16 08:14 AM (7 years, 2 months ago)

I shook two of my colonized WBS jars and they did not recover. Should I just case the other pint size jars of colonized WBS to see if I can get anything out of them? I am going to start learning agar I guess so I can have "clean" spawn. Seems I am wasting my time using syringes to inoculate grains.


--------------------


"The time has come," the Walrus said, "To talk of many things: Of shoes--and ships--and sealing-wax-- Of cabbages--and kings-- And why the sea is boiling hot-- And whether pigs have wings."


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Invisibler.lutece
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Re: G2G question [Re: Cagney]
    #23844505 - 11/18/16 08:27 AM (7 years, 2 months ago)

Quote:

Cagney said:
I am going to start learning agar I guess so I can have "clean" spawn. Seems I am wasting my time using syringes to inoculate grains.




:youthemandawg:


--------------------
One goes into an experiment knowing one might fail.
But one does not undertake an experiment knowing one HAS failed.


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