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Offlinespore-ty
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Registered: 01/21/16
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Preservatiom
    #23822192 - 11/11/16 07:35 AM (7 years, 2 months ago)

Whats up everybody so heres my dilemna this time

So ive been working on agar getting clean plates and inoculating jars with them out of 24 master jars 2 have had noticeable contams which ive tossed

But heres my question im grouping each master into its own group such as group A, B, C etc.
Ive expanded groups A,B,C to 6 jars each now my question is this

Am I wasting time expanding these untested races out without testing them

If im to test them how should I save the race if I like it

Ive thought about continuing expanding each one then use 5 jars to make a mono and save a a jar for petris ?


Edited by spore-ty (11/11/16 07:37 AM)


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OfflineThe Mycologist
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Registered: 05/06/16
Posts: 3,024
Last seen: 30 days, 9 hours
Re: Preservatiom [Re: spore-ty]
    #23822207 - 11/11/16 07:43 AM (7 years, 2 months ago)

Just put A,B, and C on their own petris, let them grow a bit then throw them in the fridge. Once you test which one does the best you can go back to the petris and further isolate the best one or you can take a clone from the biggest fruit that grows and go from there.


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


Edited by The Mycologist (11/11/16 07:46 AM)


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Offlinespore-ty
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Re: Preservatiom [Re: The Mycologist]
    #23822234 - 11/11/16 08:00 AM (7 years, 2 months ago)

Thanks thats also what I was thinking another question can you only put monocultures/isolates in master slants?


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OfflineThe Mycologist
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Re: Preservatiom [Re: spore-ty]
    #23822266 - 11/11/16 08:20 AM (7 years, 2 months ago)

It takes a lot of transfers to get to a monoculture. You can put anything you want to store for use later in the slant, its just another way to store inoculate. In your situation (depending on how many transfers you did) it probably wont be an isolate but it will be better than ms.


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


Edited by The Mycologist (11/11/16 08:22 AM)


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Invisiblefreespeech
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Registered: 12/12/08
Posts: 1,745
Loc: PNW
Re: Preservatiom [Re: The Mycologist]
    #23822480 - 11/11/16 09:56 AM (7 years, 2 months ago)

You can put any culture you want into a slant. Why not?


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Offlinespore-ty
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Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
Re: Preservatiom [Re: freespeech]
    #23822640 - 11/11/16 11:00 AM (7 years, 2 months ago)

I did roughly 5 transfers and got some pretty cool looking plates




And ok sweet thanks guys


Edited by spore-ty (11/11/16 11:00 AM)


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