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OfflineJusTUNEin
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Please Help a NOOB - is this Contamination?
    #23821599 - 11/11/16 12:05 AM (7 years, 2 months ago)

Thank you in advance for your time and help!  Its much appreciated.  Below are two cases (with two pictures for each case).  In case 1, the jar has been inoculated for about a month, and the jar has really, really small, isolated incidences of colored flecks (it looks like a piece of vermiculite simply changed color).  In case 2, the jar was inoculated about 2.5 weeks ago - there is some definite brown growth - again very slight.  I am wondering if these are contamination, and what the possible remedies might be. Both cases are PF Tek with brown rice meal and vermiculite.

Case 1:



Case 2:



Thanks again for your advice and support!


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Invisiblejoey
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Re: Please Help a NOOB - is this Contamination? [Re: JusTUNEin]
    #23821676 - 11/11/16 12:58 AM (7 years, 2 months ago)

First case looks decent.
The specks are verm pressed against the glass.
How thick was your dry verm layer?
What are your temps like?

Second case...
How are you going to get the cake out of the jar?
Could be metabolites, could be contams, who knows?

edit- seeing the amount of open air space in that one jar, you will probably have to toss it.

How did you inoculate those jars?


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OfflinecronicrFacebook
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Re: Please Help a NOOB - is this Contamination? [Re: joey]
    #23821822 - 11/11/16 03:35 AM (7 years, 2 months ago)

Case 2 is toast


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OfflineIAMNOTALLAMA
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Re: Please Help a NOOB - is this Contamination? [Re: cronicr]
    #23821892 - 11/11/16 04:30 AM (7 years, 2 months ago)

Looks like bacterial contamination on the top half of the 3rd and 4th pic. Shame.

Take the term 'field capacity' seriously. Needs to be dryer than you'd think. Better luck next time :/

Why don't you have a dry vermiculite barrier at the top of your jars?

Also I slightly edited your third pic.



Lastly, you can't use tapered jars with PF cakes. As Joey said, how the fuck do you expect to get the cakes out of the jar?



Edited by IAMNOTALLAMA (11/11/16 04:52 AM)


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Offlinepolishfarmer
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Re: Please Help a NOOB - is this Contamination? [Re: IAMNOTALLAMA]
    #23821904 - 11/11/16 04:41 AM (7 years, 2 months ago)

I dunno case 2 doesn't look that bad to me... looks more like metabolites. but would metabolites show up on a 2.5 week culture?

It could still be viable. that myc looks pretty healthy other than that discoloration which doesnt look too terrible looks like a piece of the medium is just more saturated with water or something. the myc is def taking over though it seems.

spawn it in a separate isolated place and see


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InvisibleMad Season
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Re: Please Help a NOOB - is this Contamination? [Re: polishfarmer]
    #23822126 - 11/11/16 07:07 AM (7 years, 2 months ago)

Quote:

IAMNOTALLAMA said:
Looks like bacterial contamination on the top half of the 3rd and 4th pic. Shame.




Are you kidding? That is moldy AF.

Quote:

polishfarmer said:
I dunno case 2 doesn't look that bad to me... looks more like metabolites. but would metabolites show up on a 2.5 week culture?

It could still be viable. that myc looks pretty healthy other than that discoloration which doesnt look too terrible looks like a piece of the medium is just more saturated with water or something. the myc is def taking over though it seems.

spawn it in a separate isolated place and see



:justno:

Quote:

Kizzle said:
Sectoring - When two different fungal species are growing in the same substrate they ternd to form distinct borders between the two mycelia. Often a line of metabolites may be seen where the two fungi meet. Hourglass shaped mold sectors in PF jars are almost always the result of a compromised dry vermiculite layer whereas circular mold colonies are usually the result of a mold growing from one of the inoculation points.






That top portion is a different mold dude.. definitely don't spawn/birth anything and follow the TCs advice. Toss the bitch.


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OfflineJusTUNEin
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Re: Please Help a NOOB - is this Contamination? [Re: JusTUNEin]
    #23834328 - 11/15/16 01:21 AM (7 years, 2 months ago)

Thanks for the help!  I tossed the small jar (for the best, since like you said, I prolly couldn't the cake out anyways).  The second jar looks good - here's hoping!

Cheers


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OfflineHamHead
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Re: Please Help a NOOB - is this Contamination? [Re: IAMNOTALLAMA]
    #23834332 - 11/15/16 01:25 AM (7 years, 2 months ago)

Quote:

IAMNOTALLAMA said:
Looks like bacterial contamination on the top half of the 3rd and 4th pic. Shame.

Take the term 'field capacity' seriously. Needs to be dryer than you'd think. Better luck next time :/

Why don't you have a dry vermiculite barrier at the top of your jars?

Also I slightly edited your third pic.



Lastly, you can't use tapered jars with PF cakes. As Joey said, how the fuck do you expect to get the cakes out of the jar?






Whoa, that's creepy as fuck, looking back to the original posted pic, looks like a demon there.
:shocking:


--------------------
The Italian researchers’ findings, published by the INT’s scientific magazine Tumori Journal, show 11.6% of 959 healthy volunteers enrolled in a lung cancer screening trial between September 2019 and March 2020 had developed coronavirus antibodies well before February.

https://www.reuters.com/article/us-health-coronavirus-italy-timing-idUSKBN27V0KF

This online first version has been peer-reviewed, accepted and edited,  but not formatted and finalized with corrections from authors and proofreaders

https://www.icandecide.org/


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OfflineJusTUNEin
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Re: Please Help a NOOB - is this Contamination? [Re: joey]
    #23834554 - 11/15/16 06:29 AM (7 years, 2 months ago)

Thanks for the help.

The dry verm layer was about 3/4 of an inch at the top.  The temperature ranges from about 70 - 80 F. 

Both jars were inoculated with spore syringes.  I burnt the needle with a candle until red hot, then let it cool. I realize the jar won't work - I live in a remote area where jars are not readily available.  I had a whole plan to re-use some of the cake to make more cakes, but that doesn't matter now since (4 days later) its clear that case 2 is another mold, and I've tossed it.

Thanks again


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OfflineJusTUNEin
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Re: Please Help a NOOB - is this Contamination? [Re: Mad Season]
    #23834556 - 11/15/16 06:32 AM (7 years, 2 months ago)

Thank you for your responses.  I was glad to look at the pictures you attached to learn more.  I am curious - you said "Hourglass shaped mold sectors in PF jars are almost always the result of a compromised dry vermiculite layer."  In the case of those hourglasses in your pictures - does this mean that the cake is compromised?  This hasn't happened to me yet, but it might in the future and such things are good to know:cool:


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