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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Re: Agar plates - which one to inoculate with and which one to transfer from? [Re: bodhisatta]
#23819517 - 11/10/16 11:53 AM (7 years, 2 months ago) |
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Good information thanks everyone
-------------------- KSSS And PE WBS.
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lukehighwalker710
Cloud 9 Dweller



Registered: 03/04/16
Posts: 609
Loc: out there
Last seen: 6 years, 22 days
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Re: Agar plates - which one to inoculate with and which one to transfer from? [Re: bodhisatta]
#23819559 - 11/10/16 12:03 PM (7 years, 2 months ago) |
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Rather than go 1 plate to GM, and g2g to 10 jars. I have found it effective to go agar to Li, and directly to 10 jars. Same end result, just getting there faster.
Additionally, when dealing with spawn that is a little bacterial you don't have to worry about g2g weakening and contaming jars.
My first grow was agar to grain, tiger drop 1.0 & 2.0 (tic tac toe) taking one plate per jar. Second go around I used Li and 1 plate in 10 jars. Definitely will be using Li for my 3rd go too
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Highwalker
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
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Re: Agar plates - which one to inoculate with and which one to transfer from? [Re: Moabfighter]
#23819569 - 11/10/16 12:05 PM (7 years, 2 months ago) |
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I'd rather g2g but I've been having issues with bacterial spawn for Some reason. Dropping plates isn't that bad but it is slow as hell for me. I need quick colonization apparently and Li seems like it would solve my problem.
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Re: Agar plates - which one to inoculate with and which one to transfer from? [Re: lukehighwalker710]
#23819572 - 11/10/16 12:06 PM (7 years, 2 months ago) |
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You do the blenderless LI? Seems easy enough for a beginner... add to jar of sterile water (boil water, pour in jar, put lid on, wait to cool?) add agar plate, shake, pour into ten jars?
-------------------- KSSS And PE WBS.
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Agar plates - which one to inoculate with and which one to transfer from? [Re: Kenetic]
#23819587 - 11/10/16 12:09 PM (7 years, 2 months ago) |
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Quote:
kenetic said: I'd rather g2g but I've been having issues with bacterial spawn for Some reason. Dropping plates isn't that bad but it is slow as hell for me. I need quick colonization apparently and Li seems like it would solve my problem.
prep your grains better/differently so they're dry on the outside, PC them for longer, get better at doing sterile work. LI wont solve problems with bacteria etc, its just another step which helps you if you got your shit down but can fuck up more for you if you dont.
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lukehighwalker710
Cloud 9 Dweller



Registered: 03/04/16
Posts: 609
Loc: out there
Last seen: 6 years, 22 days
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Re: Agar plates - which one to inoculate with and which one to transfer from? [Re: Moabfighter]
#23819623 - 11/10/16 12:18 PM (7 years, 2 months ago) |
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Quote:
Moabfighter said: You do the blenderless LI? Seems easy enough for a beginner... add to jar of sterile water (boil water, pour in jar, put lid on, wait to cool?) add agar plate, shake, pour into ten jars?
Um. I definitely PCd my jar of water for Li for like 30-40 min. But yes blenderless and yep thats the basic idea.
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Highwalker
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 8 hours
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Re: Agar plates - which one to inoculate with and which one to transfer from? [Re: spacechildo]
#23819678 - 11/10/16 12:34 PM (7 years, 2 months ago) |
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How about this for 2nd transfer.

The mycelium hand
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Agar plates - which one to inoculate with and which one to transfer from? [Re: Edmunter]
#23819688 - 11/10/16 12:36 PM (7 years, 2 months ago) |
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why do you take such large transfers? are most of them just agar or do you take a large chunk of myc? remember the leading edge is barely visible.
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 8 hours
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Re: Agar plates - which one to inoculate with and which one to transfer from? [Re: spacechildo]
#23819704 - 11/10/16 12:42 PM (7 years, 2 months ago) |
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I just wanted to get those hand like strains on top. This is 2nd transfer from spore germination. Ive never got this far so quick
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spirit_shadow
Feature not a bug



Registered: 08/15/11
Posts: 25,674
Last seen: 6 hours, 49 minutes
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Re: Agar plates - which one to inoculate with and which one to transfer from? [Re: Edmunter]
#23819992 - 11/10/16 02:31 PM (7 years, 2 months ago) |
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Li is amazing ime
-------------------- ERROR 418 IM A TEAPOT.....(this account is automated, all posts related to illegal activities or advice thereof are strictly from numerous online sites and are for informational purposes only)- Circa 2011 Ban lotto
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Re: Agar plates - which one to inoculate with and which one to transfer from? [Re: spirit_shadow]
#23819994 - 11/10/16 02:32 PM (7 years, 2 months ago) |
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Quote:
spirit_shadow said: Li is amazing ime 
How you doing it man?
-------------------- KSSS And PE WBS.
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spirit_shadow
Feature not a bug



Registered: 08/15/11
Posts: 25,674
Last seen: 6 hours, 49 minutes
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Re: Agar plates - which one to inoculate with and which one to transfer from? [Re: Moabfighter]
#23819998 - 11/10/16 02:34 PM (7 years, 2 months ago) |
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Well I use pastys so once I have a clean plate I just inject it with sterile water and use the sterilized needle to kinda scratch the surface of the myc and suck it back up.
-------------------- ERROR 418 IM A TEAPOT.....(this account is automated, all posts related to illegal activities or advice thereof are strictly from numerous online sites and are for informational purposes only)- Circa 2011 Ban lotto
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Agar plates - which one to inoculate with and which one to transfer from? [Re: spirit_shadow]
#23820014 - 11/10/16 02:43 PM (7 years, 2 months ago) |
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Do you sterilize the water within the syringe? Or do you sterilize it in the jar and pour it inside the pastyplate?
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
Edited by AlCapone2k (11/10/16 02:43 PM)
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spirit_shadow
Feature not a bug



Registered: 08/15/11
Posts: 25,674
Last seen: 6 hours, 49 minutes
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Re: Agar plates - which one to inoculate with and which one to transfer from? [Re: AlCapone2k]
#23820121 - 11/10/16 03:28 PM (7 years, 2 months ago) |
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I sterilize the water in the syringe.
1. Bring pot of water to boil.
2.pull syringe full of water.
3. Squrit that into the SINK. NOT the pot with boiling water.
4. Repeat steps 2 and 3 about 4 times.
5. Let sit till cool.
-------------------- ERROR 418 IM A TEAPOT.....(this account is automated, all posts related to illegal activities or advice thereof are strictly from numerous online sites and are for informational purposes only)- Circa 2011 Ban lotto
Edited by spirit_shadow (11/10/16 03:36 PM)
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Agar plates - which one to inoculate with and which one to transfer from? [Re: spirit_shadow]
#23820147 - 11/10/16 03:36 PM (7 years, 2 months ago) |
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No concerns about heat resistant microbes that are pushed back into the water from the outer edged of the pot?
IIRC that was one of the problems of self made spore syringes?!
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Agar plates - which one to inoculate with and which one to transfer from? [Re: AlCapone2k]
#23820203 - 11/10/16 03:52 PM (7 years, 2 months ago) |
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nah heat and humid air rises. away from your pot.
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frankwhite85
Stranger


Registered: 04/18/16
Posts: 305
Last seen: 1 month, 6 days
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Re: Agar plates - which one to inoculate with and which one to transfer from? [Re: bodhisatta]
#23820332 - 11/10/16 04:44 PM (7 years, 2 months ago) |
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The first plate is not very healthy growth the second is healthy tomentose growth, I wouldn't use the first one at all and use the 2nd one to either make more transfers or put it to grain
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Agar plates - which one to inoculate with and which one to transfer from? [Re: frankwhite85]
#23820436 - 11/10/16 05:32 PM (7 years, 2 months ago) |
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So toss the first one?
Maybe you are right, since another transfer wouldn't change anything. Either there is a hidden contamination (what I don't think) or it's just bad genetics, hm?
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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spirit_shadow
Feature not a bug



Registered: 08/15/11
Posts: 25,674
Last seen: 6 hours, 49 minutes
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Re: Agar plates - which one to inoculate with and which one to transfer from? [Re: AlCapone2k]
#23820478 - 11/10/16 05:43 PM (7 years, 2 months ago) |
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Do what you want
-------------------- ERROR 418 IM A TEAPOT.....(this account is automated, all posts related to illegal activities or advice thereof are strictly from numerous online sites and are for informational purposes only)- Circa 2011 Ban lotto
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Agar plates - which one to inoculate with and which one to transfer from? [Re: AlCapone2k]
#23820485 - 11/10/16 05:44 PM (7 years, 2 months ago) |
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time to put your research skills to use capone! who to trust....?
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