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Offlineafflictis
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First attempt at agar
    #23818287 - 11/10/16 03:38 AM (7 years, 2 months ago)



First four plates just seeing if these are healthy or bacterial. I know they are sloppy. Thanks for the help


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Offlineafflictis
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Re: First attempt at agar [Re: afflictis]
    #23819984 - 11/10/16 02:28 PM (7 years, 2 months ago)

Anyone?


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OfflinePinpapa
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Re: First attempt at agar [Re: afflictis]
    #23823127 - 11/11/16 02:01 PM (7 years, 2 months ago)

They look ok now transfer from the leading edge.


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Offlineafflictis
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Re: First attempt at agar [Re: Pinpapa]
    #23823326 - 11/11/16 03:14 PM (7 years, 2 months ago)

Leading edge? As in where it's thickest?


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OfflinePinPornProducer
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Re: First attempt at agar [Re: afflictis]
    #23823339 - 11/11/16 03:17 PM (7 years, 2 months ago)

Lots of bacteria in those


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Offlineafflictis
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Re: First attempt at agar [Re: PinPornProducer]
    #23823345 - 11/11/16 03:19 PM (7 years, 2 months ago)

How can you tell which is bacteria


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Offlineafflictis
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Re: First attempt at agar [Re: afflictis]
    #23824505 - 11/11/16 11:30 PM (7 years, 2 months ago)

Anyone have any advice on how to tell what is bacteria and what is healthy mycelium


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Re: First attempt at agar [Re: afflictis]
    #23824624 - 11/12/16 12:26 AM (7 years, 2 months ago)

First pic kinda right of center, take some from that leading edge.

2nd pic take from 3 o'clock

3rd plate I wouldn't fuck with

Last pic hard to tell but maybe take from the right of the top growth and maybe take from the top middle of the larger growth to the right.

When mycelium wedges out like Pac mans mouth is a very good indicator of competitor molds and bacteria. The first plate has a slimey appearance which looks like bacteria to me.

The places I told you to transfer from would be your safest bet imo


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Offlineafflictis
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Re: First attempt at agar [Re: PinPornProducer]
    #23824686 - 11/12/16 01:09 AM (7 years, 2 months ago)

Quote:

PinPornProducer said:
First pic kinda right of center, take some from that leading edge.

2nd pic take from 3 o'clock

3rd plate I wouldn't fuck with

Last pic hard to tell but maybe take from the right of the top growth and maybe take from the top middle of the larger growth to the right.

When mycelium wedges out like Pac mans mouth is a very good indicator of competitor molds and bacteria. The first plate has a slimey appearance which looks like bacteria to me.

The places I told you to transfer from would be your safest bet imo



Thanks this agar shit isn't as easy as people make it sound


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OfflinePinPornProducer
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Re: First attempt at agar [Re: afflictis]
    #23824700 - 11/12/16 01:16 AM (7 years, 2 months ago)

Eh it gets easier man. Its way easier if you just put 1 or 2 drops on the plate so you don't have a slew of growth everywhere lol. Makes it easier to manage and the less spores or spore solution you use the less mold and bacteria is passed on also


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Offlineafflictis
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Re: First attempt at agar [Re: PinPornProducer]
    #23824712 - 11/12/16 01:21 AM (7 years, 2 months ago)

I tried only putting one or two drops. How often do the syringes come contaminated cause I was using an sab and the vendor I use says the syringes come sterile


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Re: First attempt at agar [Re: afflictis]
    #23824746 - 11/12/16 01:44 AM (7 years, 2 months ago)

Mushrooms are never fruited in a sterile environment. They are exposed to mold spores from day one of spawning through their entire life cycle. I can't really say how often syringes are dirty but if I had to guess I'd say it's a 50/50 chance of actually getting a clean syringe


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Offlineafflictis
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Re: First attempt at agar [Re: PinPornProducer]
    #23824756 - 11/12/16 01:53 AM (7 years, 2 months ago)

Cause my first successful monotub was wbs spawned into sterilized cow poo coir vermiculite and I got 5 flushes without any contams and no sab. But now I can't seem to get shit to work


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Re: First attempt at agar [Re: afflictis]
    #23824763 - 11/12/16 01:57 AM (7 years, 2 months ago)

Welcome to the world of mycology :raisemyglass:


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Offlineafflictis
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Re: First attempt at agar [Re: PinPornProducer]
    #23824767 - 11/12/16 02:01 AM (7 years, 2 months ago)

Lol its a Rollercoaster of questions and emotions


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Re: First attempt at agar [Re: afflictis]
    #23824786 - 11/12/16 02:12 AM (7 years, 2 months ago)

We have all been through it and still go through it man. Just got to be patient but persistent. I threw away like 12 monos not too long ago because I had fungus gnats. Just recently had 3 months of bad spawn off and on due to a stealthy contam on agar I kept missing and was transferring it. Nothings %100 in this hobby lol, no matter how clean you think you're being. There will always be bumps in the road and some bumps are alot bigger than others


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Offlineafflictis
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Re: First attempt at agar [Re: PinPornProducer]
    #23824820 - 11/12/16 02:47 AM (7 years, 2 months ago)

I want to use agar but I can't tell the difference between healthy mycelium and bacteria especially when they are both white


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Re: First attempt at agar [Re: afflictis]
    #23825229 - 11/12/16 08:39 AM (7 years, 2 months ago)

Most molds are white or at least start out white. Bacteria is more of a liquid/creamy substance. Can be clear/yellowish/milky white... Identifying molds comes with experience.

Read this link it has some really good info on what to look for


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Offlineafflictis
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Re: First attempt at agar [Re: PinPornProducer]
    #23826210 - 11/12/16 03:24 PM (7 years, 2 months ago)

Thanks for all your help


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