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Invisiblefreespeech
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Spawning jars derived from same MS G2G master jar together - does it matter?
    #23816149 - 11/09/16 12:46 PM (7 years, 2 months ago)

Let's say I have 4 multispore grain master jars, all created from the same spore syringe. I use each of those to create 7 more jars, for a total of 28 descendant jars. I label the descendants from the first grain master as batch "A", the descendants of the second grain master as batch "B", and so on.

I use 7 jars of grain in each of my monotubs, so I'm going to create 4 tubs from my 28 grain jars. All else being equal, do you think it matters in any way whether I spawn all "A" jars to the same tub, vs. mixing jars from the various batches between the tubs? Is there any sort of "familial" relationship within each batch that could improve performance if the jars from each batch are kept together? My gut tells me no, but I'm curious if anyone has more insight into this. Keep in mind that this is MS.


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OfflineEdmunter
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Re: Spawning jars derived from same MS G2G master jar together - does it matter? [Re: freespeech]
    #23816178 - 11/09/16 12:59 PM (7 years, 2 months ago)

You are improving your chances of success if you keep them separate. 

What size are the jars?

Have you done mono tubs before?


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Invisiblefreespeech
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Re: Spawning jars derived from same MS G2G master jar together - does it matter? [Re: Edmunter]
    #23816189 - 11/09/16 01:05 PM (7 years, 2 months ago)

Yeah I generally know what I'm doing, just curious about this specific aspect of genetic lineage. Assume all the jars are healthy.


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Re: Spawning jars derived from same MS G2G master jar together - does it matter? [Re: freespeech]
    #23816220 - 11/09/16 01:17 PM (7 years, 2 months ago)

Hahaha funny as fuck were in the same exact boat.... except im doing 6 per group.

But im gonna keep em.together except for one tub where im gonna throw a jar from each group into a tub

Ill be starting a log soon

Btw my jars are from a multispore grow where i took clones to agar then clean plates to masters


Edited by spore-ty (11/09/16 01:18 PM)


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Invisiblefreespeech
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Re: Spawning jars derived from same MS G2G master jar together - does it matter? [Re: spore-ty]
    #23816224 - 11/09/16 01:20 PM (7 years, 2 months ago)

I'm curious to hear about your results. Update this thread or PM me if, you remember, once you've got the flushes from this trial.


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OfflineEdmunter
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Re: Spawning jars derived from same MS G2G master jar together - does it matter? [Re: freespeech]
    #23816281 - 11/09/16 01:43 PM (7 years, 2 months ago)

I dont think it would make much difference.


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OfflineMushierage
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Re: Spawning jars derived from same MS G2G master jar together - does it matter? [Re: Edmunter]
    #23816431 - 11/09/16 02:37 PM (7 years, 2 months ago)

The only problem is that down the line, you might have a bit of bacteria.  Nothing horrible, but still enough to affect yields a little.  I wouldn't expand MS but maybe one time, after that you're just asking for a bacterial ass kicking in your tubs. 

If you want to expand multiple times, do it with an isolate, or at the very least from clean agar wedges.


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Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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InvisibleTheMadHatter420
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Re: Spawning jars derived from same MS G2G master jar together - does it matter? [Re: Mushierage]
    #23816505 - 11/09/16 03:00 PM (7 years, 2 months ago)

I have some pint MS that I want to expand. Should I just go to quarts, 4 from each of 3 pints. Colonize those 12 quarts. Then just put that 12 into a mono, case and fruit? I am picking up that trying to go one more step and do a 1:1 spawn/straw mono would be a bad idea.


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OfflineMushierage
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Re: Spawning jars derived from same MS G2G master jar together - does it matter? [Re: TheMadHatter420]
    #23819056 - 11/10/16 10:01 AM (7 years, 2 months ago)

3 pints is a small amount to expand... but, you might be able to do that.  It might take awhile that way, because of the low g2g volume, but thats okay.

I would use that to start 3 monos.  4 quarts each, 1:1 or 1:2 depending on the volume of FC you're using for them. 

You could even do 2 monos with 6 quarts each, 1:1 or 1:2. I've been thinking about doing the same, except with a grow tent and longer trays that will hold a lot more spawn and sub.


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Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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InvisibleTheMadHatter420
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Re: Spawning jars derived from same MS G2G master jar together - does it matter? [Re: Mushierage]
    #23819107 - 11/10/16 10:15 AM (7 years, 2 months ago)

Knowing I am using MS and that there HAS t be some bacteria in there makes me think I should just expand to grain and then just case and fruit the grain. Then again I have read on here some people will use it to expand to bulk using bulk substrate and get a couple flushes. I know this has a better chance for bigger yield than just casing and fruiting the grains, BUT it also has a better chance of contamination and failing. I figure 1/4 pint of good colonized grain per quart(for expansion) should be enough inoculation points. I mean I read about 1 quart being able to expand to 10. So 1.5 quart expanding to 12 should work.


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Edited by TheMadHatter420 (11/10/16 10:16 AM)


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OfflineMushierage
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Re: Spawning jars derived from same MS G2G master jar together - does it matter? [Re: TheMadHatter420]
    #23819144 - 11/10/16 10:24 AM (7 years, 2 months ago)

I wouldn't ever do that.  Fruiting the grain has never been a real successful endeavor for me, and that is mainly because grain does not hold water very well at all, even if cased with something very voluminous.  Doing 6 myco qts to 6 lbs of coir/verm/gypsum will give you a very large tub and a very large first and second flush as well.  It would colonize pretty quick too, 5-7 days or you could put into fruiting condition the day you spawn.  Doesn't matter.

There is always bacteria in your spawn.  -Always-.  The question of whether it does well or not is a question of how much bacteria you have, and what kinds.  Are they aggressive, semi-aggressive, passive, or beneficial?  You can tell just by looks at the mycelium, smelling it, if you know what you're looking for.


--------------------
Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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Re: Spawning jars derived from same MS G2G master jar together - does it matter? [Re: TheMadHatter420]
    #23819149 - 11/10/16 10:26 AM (7 years, 2 months ago)

You can expand a quart much more than that if you dont mind waiting a couple of days more.  Growing from  pin clone expandede to a few plates is the best way ive found.  If you get the first pin to pop up you know its a fast fruiter and that speeds all grows up.

Also you know its clean


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OfflineMushierage
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Re: Spawning jars derived from same MS G2G master jar together - does it matter? [Re: Edmunter]
    #23819162 - 11/10/16 10:28 AM (7 years, 2 months ago)

Quote:

Edmunter said:
You can expand a quart much more than that if you dont mind waiting a couple of days more.  Growing from  pin clone expandede to a few plates is the best way ive found.  If you get the first pin to pop up you know its a fast fruiter and that speeds all grows up.




Yes, but my point was expanding too much from a MS master isn't the way to go.  All your shit is eventually gonna trich out and make you cry.

If you're going to expand, only expand 1 maybe 2 time with MS.  Don't go anymore than that or you are really gonna regret it, I should know.  It happened to me once.


--------------------
Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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OfflineEdmunter
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Re: Spawning jars derived from same MS G2G master jar together - does it matter? [Re: Mushierage]
    #23819173 - 11/10/16 10:30 AM (7 years, 2 months ago)

Quote:

Mushierage said:
Quote:

Edmunter said:
You can expand a quart much more than that if you dont mind waiting a couple of days more.  Growing from  pin clone expandede to a few plates is the best way ive found.  If you get the first pin to pop up you know its a fast fruiter and that speeds all grows up.




Yes, but my point was expanding too much from a MS master isn't the way to go.  All your shit is eventually gonna trich out and make you cry.

If you're going to expand, only expand 1 maybe 2 time with MS.  Don't go anymore than that or you are really gonna regret it, I should know.  It happened to me once.



:whathesaid:

Yes, best to clone and grow from that.


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InvisibleTheMadHatter420
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Re: Spawning jars derived from same MS G2G master jar together - does it matter? [Re: Edmunter]
    #23819971 - 11/10/16 02:24 PM (7 years, 2 months ago)

I plan to clone as soon as a few of these cakes in my FC fruit. I have been using brown rice, hydrates well, with about 25% vermiculite in it. I plan to do this ffor the quarts. I figured regardless of the route I take I will only get a couple flushes, at best, because I started from MS.


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Invisiblefreespeech
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Re: Spawning jars derived from same MS G2G master jar together - does it matter? [Re: TheMadHatter420]
    #23819986 - 11/10/16 02:28 PM (7 years, 2 months ago)

This isn't relevant to this thread. You should start a new thread.


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Offlinespacechildo
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Re: Spawning jars derived from same MS G2G master jar together - does it matter? [Re: freespeech]
    #23820064 - 11/10/16 03:04 PM (7 years, 2 months ago)

yes you can mix all kinds of MS jars together in both tubs and grains and whatnots, keep species separate of course but dont listen to people who makes you think you need to keep myc from 1 print away from another.

g2g away, spawn away!

if your jars are bacterial to the point it looks off, dont g2g. just spawn.

mushierage loves to guess so dont even bother reading his posts.


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