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InvisibleTheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
How often to I change poly fill in jar lids?
    #23816101 - 11/09/16 12:28 PM (7 years, 2 months ago)

The search returned nothing good, only strange stuff. So I have been using wide mouth 1/2 pint jars for agar work. I have now used up all new ones and have to reuse some now. I had drilled holes and packed with poly fill for GE. Do I pull out and replace poly fill each and every time? If now what about washing lids, will the poly fill hold water? I got some 3M micro pore tape and am thinking about doing a couple layers of that over the holes instead of poly fill? Any advice or recommendations would be appreciated. I just do not want to spread contam to new agar plates.


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Edited by TheMadHatter420 (11/09/16 12:31 PM)


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Offlineenlightenment
alchemist
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Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 8 months, 21 days
Re: How often to I chnage poly fill in jar lids? [Re: TheMadHatter420]
    #23816103 - 11/09/16 12:30 PM (7 years, 2 months ago)

You can it use multiple times.
Some people have success with micropore tape. I don't like it.
I would change the polyfill every time if you inoculate through it. If you don't poke a needle through the polyfill i would change it when it starts to get yellow/brown.


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InvisibleTheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
Re: How often to I chnage poly fill in jar lids? [Re: enlightenment]
    #23816109 - 11/09/16 12:32 PM (7 years, 2 months ago)

Ok any advice for do's and don'ts for washing/ cleaning these lids with it still stuck in there?


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Offlineenlightenment
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Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 8 months, 21 days
Re: How often to I chnage poly fill in jar lids? [Re: TheMadHatter420]
    #23816122 - 11/09/16 12:35 PM (7 years, 2 months ago)

The polyfill should be dry when you load your jars into the PC. It is easy to clean the lids. You can use a wet towel to clean the lids. Polyfill is a very cheap filter material. If you have problems to clean the lids while it is stuffed in the holes you can change it after every use.


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