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Offlinekeifnnugs
Master fucker
Registered: 11/27/15
Posts: 335
Last seen: 26 days, 8 hours
Agar transfers?
    #23813539 - 11/08/16 05:53 PM (7 years, 2 months ago)

When I start a print or some drops of a syringe on agar I will usually let them germ and get a nice size before I start making transfers.

My question is do you need to make transfers from your starting plate? If your starting plate is clean can you just let it grow out and tiger drop it?
My main reason for asking is I don't want to waste agar and do needless transfers since I'm just going to put on grain to coir and fruit to clone the best fruits

I usually will make 4-6 plates from my original plate of the strongest growth then take the strongest from those to make another transfer then tiger drop. Seems I can get to the fruits and cloning faster if I take the first clean plate


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OfflinecronicrFacebook
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Registered: 08/07/11
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Re: Agar transfers? [Re: keifnnugs]
    #23813555 - 11/08/16 05:59 PM (7 years, 2 months ago)

It's good practice to make transfers...often times what appears clean really isn't.


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlinekeifnnugs
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Registered: 11/27/15
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Re: Agar transfers? [Re: cronicr]
    #23813586 - 11/08/16 06:07 PM (7 years, 2 months ago)

Thanks for your response cornicr!
How many transfers would be sufficient before calling it clean?

I usually go for the thickest fastest growth.. Anything else to look for? Or are you only going for a clean culture so that you can clone and fruit?


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OfflinecronicrFacebook
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Re: Agar transfers? [Re: keifnnugs]
    #23813616 - 11/08/16 06:18 PM (7 years, 2 months ago)

A clean culture is your biggest thing...you won't see much for sector in and what not for a few transfers anyway.


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlinekrypto2000
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Re: Agar transfers? [Re: keifnnugs]
    #23813622 - 11/08/16 06:18 PM (7 years, 2 months ago)

At this point I'd just go with the fastest. Eventually you will see rhizomorphic growth and sectoring after a lot of transfers, that's how you can truly tell it's clean because it grows in an even pattern. I wouldn't necessarily go that far though as you can have fruit by the time that happens, it often takes a while. I would do 1-2 transfers maybe and take it to some grain from there. While it's growing I'd continue cleaning it up and making transfers to get practice as well as to narrow it down just in case the initial one wasn't clean. Once you can clone some good fruits you might want to abandon it, but at the very least practicing will help ensure you clone that fruit cleanly and get a good culture from that. Agar and dishes are cheap, don't worry about using too many at this point.


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Invisiblemorty422
Wuba-luba-dub-dub!
I'm a teapot

Registered: 07/06/16
Posts: 988
Re: Agar transfers? [Re: keifnnugs]
    #23813630 - 11/08/16 06:19 PM (7 years, 2 months ago)

Quote:

keifnnugs said:
Thanks for your response cornicr!
How many transfers would be sufficient before calling it clean?

I usually go for the thickest fastest growth.. Anything else to look for? Or are you only going for a clean culture so that you can clone and fruit?




It depends on what you're after.

Trying to get a monoculture from a MS syringe is going to take you quite a while. I just began fruiting my first monoculture and it took me a few months to get here.
Every time I thought I had one, I saw a new sector form in the (what I wanted to be) final transfer :facepalm:.

The above is definitely something you can do on the side while you are fruiting your clones!

Clean up the MS plates a bit until you know what you have is clean. Then toss it in your grain jars-grain master-whatever you want and fruit a tub. Take a live tissue piece (clone) from one of the best looking fastest growing fruits and place it into agar.
(I like taking the whole fruit into the SAB and attempt to keep the sample process as sterile as possible.)

Then transfer that clone plate a few times to make sure it's clean and use it in whatever you wish.

Hope that helps you!

:cheers:


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Offlinekeifnnugs
Master fucker
Registered: 11/27/15
Posts: 335
Last seen: 26 days, 8 hours
Re: Agar transfers? [Re: morty422]
    #23813683 - 11/08/16 06:34 PM (7 years, 2 months ago)

Thanks for the help everyone..

Morty I actually have some Texas yellow cap that are in there like 7th transfer and I feel like I'm never going to get a monoculture lol..
i usually pick 2 good sections and transfer those.. But it got me thinking what if Ido all this work to finally fruit it and turns out it's shit lol..
That why I figure do one transfer till clean so I can fruit leaving me with multicultural to see what pops up to clone..

Can thank pasty enough for his pasty plate idea.. To have to order plates would be a mother


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OfflineAlCapone2k
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Registered: 10/06/16
Posts: 842
Loc: Germany Flag
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Re: Agar transfers? [Re: keifnnugs]
    #23813725 - 11/08/16 06:53 PM (7 years, 2 months ago)

I would recommend to make at least 3 transfers, just to make sure you have a clean culture. Then put some clean plates at 50% growth in a zip bag in your fridge to have some backup plates.

Then start to fruit and try to get a mono from cloning.

Two transfers aren't enough IMO, cause there could be some contamination hiding ore something. Eventhoug almost all of my MS to Agar Plates look quite clean, I make three or four transfers just to make sure. Plates and Agar are so cheap ...

This is my plan :smile:


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


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