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OfflineAlCapone2k
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Oyster Rye Jar - mycelium or cobweb?
    #23812318 - 11/08/16 10:34 AM (7 years, 2 months ago)

Hey guys,

I have a rye jar inoculated with a agar wedge of oyster myelium. The agar plate was clean, so I used almost the whole plate to inoculate (cust cut out the mycelium part of the plate, the edges stayed in the petri)



Unfortunately I wasn't able to get the agar wedge sticking to the glas so I was forced to "roll" the jar a bit so that the agar wedge was covered with rye berries.

Now I see this white stuff on the top of the rye far away from the agar wedge. It could be mycelium which got there because I had to "roll" the jar a bit, so the berries there were inoculated with mycelium.

But it also could be cobweb or other mold.

It grows almost the same speed like the myeclium around the agar wedge and looks white and clean, but a bit "fluffy" and more hairy.


What do you think?


Another question: Is it time to shake or better wait some days?


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


Edited by AlCapone2k (11/08/16 10:35 AM)


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Re: Oyster Rye Jar - mycelium or cobweb? [Re: AlCapone2k]
    #23812579 - 11/08/16 12:22 PM (7 years, 2 months ago)

It doesn't look good to me..


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OfflineAlCapone2k
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Re: Oyster Rye Jar - mycelium or cobweb? [Re: Mad Season]
    #23812603 - 11/08/16 12:30 PM (7 years, 2 months ago)

yeah, seems so actually. I thought it might have come from the fact that the agar wedge was moved aroung.

But actuall I can't imagine how mold has come inside the jar, I used an SAB and never had any problems with mold before. But I had one problem during SAB work, I used a bit too much of soap water for the walls so maybe a soap water drop fell into the jar or somethin. But this would explain bacteria, not mold, right?


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


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Re: Oyster Rye Jar - mycelium or cobweb? [Re: AlCapone2k]
    #23812611 - 11/08/16 12:31 PM (7 years, 2 months ago)

Mold spores can get into water droplets quite easily :wink:


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OfflineAlCapone2k
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Re: Oyster Rye Jar - mycelium or cobweb? [Re: Mad Season]
    #23812724 - 11/08/16 12:59 PM (7 years, 2 months ago)

If it was mold, how will it develop? Get green? Grow faster than mycelium?


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


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Re: Oyster Rye Jar - mycelium or cobweb? [Re: AlCapone2k]
    #23812726 - 11/08/16 01:00 PM (7 years, 2 months ago)

Who knows. Doubtful it'll go green tho.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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OfflineAlCapone2k
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Re: Oyster Rye Jar - mycelium or cobweb? [Re: AlCapone2k]
    #23813058 - 11/08/16 02:56 PM (7 years, 2 months ago)

Ok, have any hint how I can ensure it's mold and not mycelium?


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


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Re: Oyster Rye Jar - mycelium or cobweb? [Re: AlCapone2k]
    #23815667 - 11/09/16 09:21 AM (7 years, 2 months ago)

Well wait a bit and check back. If the morphology is all wrong, it's a mold. It's much easier to tell on agar. Here's 2 white molds, aspergillus, and 2 species of bacteria on agar:



Just by looking at the morphology you can tell which is the right mycelium, and that's the one I transferred from. Ended up with a great clean culture from such a shitty syringe.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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OfflineAlCapone2k
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Re: Oyster Rye Jar - mycelium or cobweb? [Re: Mad Season]
    #23815748 - 11/09/16 10:00 AM (7 years, 2 months ago)

Ok fine, I just watched it and I don't know, it looks like mycelium, just a bit more hairy which could be because its on the top and not pushed to the glass of the jar.

Since it was "shaking time" I gave the jar a nice shake and now I'll wait what will happen during recolonization.

Or was it totally stupid to shake it?


P.S: The very dense white spot is the mycelium on your pic, right? :laugh:


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


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OfflineThe Mycologist
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Re: Oyster Rye Jar - mycelium or cobweb? [Re: AlCapone2k]
    #23815758 - 11/09/16 10:03 AM (7 years, 2 months ago)

Top left is the mycelium, you can see he took transfers from it


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OfflineAlCapone2k
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Re: Oyster Rye Jar - mycelium or cobweb? [Re: The Mycologist]
    #23818302 - 11/10/16 03:58 AM (7 years, 2 months ago)

Hey guys,

I have four other jars which haven't had any suspicious parts.

Inoculated them on 29th of October, first Shake on 4th, second one on 8th, so two days ago.

As you can see the myeclium recovered quite fast. It looks a bit fluffy  but I think this isn't unnormal for oyter myc.


Shall I spawn now or shake once more? Or wait a couple of day before spawning?




Regards.


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


Edited by AlCapone2k (11/10/16 03:58 AM)


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Re: Oyster Rye Jar - mycelium or cobweb? [Re: AlCapone2k]
    #23818766 - 11/10/16 08:51 AM (7 years, 2 months ago)

I'd spawn them. Oysters are aggressive fuckers


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflineAlCapone2k
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Re: Oyster Rye Jar - mycelium or cobweb? [Re: Mad Season]
    #23818772 - 11/10/16 08:52 AM (7 years, 2 months ago)

Yeah, I'll give them just one day and then spawn them.

Do you shake them directly before spawning?


P.S: So you also don't see any contamn in this three, right? :smile:


Regards


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


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Re: Oyster Rye Jar - mycelium or cobweb? [Re: AlCapone2k]
    #23818783 - 11/10/16 08:55 AM (7 years, 2 months ago)

Nothing blaringly obvious. Like I said I haven't had enough exposure to know oyster morphology like I do cubensis. If that were cubensis I'd say it looks slightly bacterial, but it isn't lol. Besides oysters rip through bacteria much better than cubensis ever could


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflineAlCapone2k
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Re: Oyster Rye Jar - mycelium or cobweb? [Re: Mad Season]
    #23818905 - 11/10/16 09:26 AM (7 years, 2 months ago)

Yes, I have noticed that, Oyster is quite easy to work with, cause it's fast and strong :smile:
Therefore I'm really looking forward to a nice bag grow with straw pellets.

I'll start it tomorrow :smile:

Thank you for your advide.


Regards


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


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OfflineAlCapone2k
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Re: Oyster Rye Jar - mycelium or cobweb? [Re: AlCapone2k]
    #23825154 - 11/12/16 08:06 AM (7 years, 2 months ago)

Ok, after the first shake (directly after the picture of my first Posting here) I let it recolonize for 3 days and then gave it a shake today again.


Picture before the shake:



Picture after the shake:






What do you think guys? Doesn't look good, hm? (don't forget it's oyster mycelium, not Cubensis) :smile:



Regards


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


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