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OfflineMycah9
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What's up with my plates?
    #23809958 - 11/07/16 02:57 PM (7 years, 3 months ago)

I'll try make this short. Are these plates fucked? Done a few successful transfers from a tissue clone, probably transferring too big of pieces. Couple of those plates I started over 10 days ago from ms syringe. Too many drops? Thanks for the help, sorry if I'm missing any info.


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OfflineKenetic
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Re: What's up with my plates? [Re: Mycah9]
    #23809971 - 11/07/16 03:02 PM (7 years, 3 months ago)

alright....so are these ms or clone tissue?  I'm gonna go with ms by the looks of them, but honestly, I can't see shit.  What I can see is that it's time to start transferring.  Who knows, you might be able to answer your own question in a few days.

Edit:  btw you definitely used enough agar lol


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OfflineMycah9
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Re: What's up with my plates? [Re: Kenetic]
    #23810000 - 11/07/16 03:11 PM (7 years, 3 months ago)

Haha right? The top 3 are transfers from tissue clone. The others are all from ms. The agar is turning white, just not seeing the typical stringy web looking mycelium I'm used to. Is that how ms to agar is supposed to look after 10 days?

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OfflineKenetic
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Re: What's up with my plates? [Re: Mycah9]
    #23810009 - 11/07/16 03:16 PM (7 years, 3 months ago)

there's a lot of variables and I'm far from an expert.  I'd say that's typical on agar in my experience.  I have yet to see strong rhizo growth on any of my agar, and to be honest with you, I think rhizo growth is overrated anyway. 

I've read that rhizo can be a result of low-nutritious agar or species-specific and can revert to and from tomentos growth from temp changes and other stuff like that.


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OfflineMycah9
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Re: What's up with my plates? [Re: Kenetic]
    #23810135 - 11/07/16 04:07 PM (7 years, 3 months ago)

Ok I feel ya. It's my first time putting spores straight to agar, so not really sure how it's supposed to look. Only done clones and transfers and expected it to look more like this


So if the plates fully colonized and everything looks the same, how do you choose which sector to transfer?

Can anybody post a pic of ms on agar?

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InvisiblebodhisattaMDiscordReddit
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Re: What's up with my plates? [Re: Mycah9]
    #23810165 - 11/07/16 04:21 PM (7 years, 3 months ago)


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OfflineMycah9
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Re: What's up with my plates? [Re: bodhisatta]
    #23810262 - 11/07/16 05:07 PM (7 years, 3 months ago)

Thanks bodhi, how far along are those? Should I keep letting these go, transfer, or start over?

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OfflinecronicrFacebook
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Re: What's up with my plates? [Re: Mycah9]
    #23810403 - 11/07/16 05:46 PM (7 years, 3 months ago)

When starting from spores I transfer as soon as possible..your not looking for much other then clean growth


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OfflineMycah9
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Re: What's up with my plates? [Re: cronicr]
    #23810951 - 11/07/16 08:12 PM (7 years, 3 months ago)

Niiiiice made my day.  So I would assume these plates are not fucked, and start transferring. Really appreciate the feedback. What sections would you take a transfer from? Everything looks the same at this point. My apologies for so many questions. Thought someone would jus tell me those are fucked, super bacterial, start over.

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