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Offlineafflictis
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Spawning jars early
    #23804160 - 11/05/16 05:57 PM (7 years, 3 months ago)

Any harm in spawning jars before they are 100%. I have 7 jars that are about 50 to 70% done they are just taking so long.

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Offlinemrmazdarx9
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Re: Spawning jars early [Re: afflictis]
    #23804168 - 11/05/16 05:59 PM (7 years, 3 months ago)

Well if not fully colonised your more open to contams


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Edited by mrmazdarx9 (11/05/16 06:31 PM)

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InvisiblemushboyMDiscord
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Re: Spawning jars early [Re: mrmazdarx9]
    #23804191 - 11/05/16 06:04 PM (7 years, 3 months ago)

all those uncolonized bits will be a contam buffet. just wait be patient. read a book. ride a bike.

how long is 'so long'?

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OfflineWeavieWonder
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Re: Spawning jars early [Re: mushboy]
    #23804631 - 11/05/16 08:11 PM (7 years, 3 months ago)

You will be waiting even longer if you spawn before 100% colonization because you will end up having to start over.  Just chill.

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Offlineafflictis
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Re: Spawning jars early [Re: mushboy]
    #23804659 - 11/05/16 08:23 PM (7 years, 3 months ago)

It's been maybe a week and a half already. B+ strain g2g transfer wbs incubating at 84f.

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OfflineWeavieWonder
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Re: Spawning jars early [Re: afflictis]
    #23804895 - 11/05/16 09:36 PM (7 years, 3 months ago)

Colonizing @ 84F might be warm enough to slow down growth.  Are using an incubator?  If so, take your jars out of there.  Room temperature (70-75F) for colonization is plenty warm.

    Also, have you shaken your grain at any point from G2G to now?  A good shake a 30 -50% colonization really speeds things up, provided your grain isn't contaminated.

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Invisiblefreespeech
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Re: Spawning jars early [Re: WeavieWonder]
    #23804975 - 11/05/16 10:10 PM (7 years, 3 months ago)

Do not ever spawn uncolonized grain. If your jars are taking a long time then something is probably wrong. You probably had bacteria in the grain master or introduced it during the g2g. Even so, your best bet is to wait it out and see if they finish colonizing before spawning.

Quote:

WeavieWonder said:
Also, have you shaken your grain at any point from G2G to now?  A good shake a 30 -50% colonization really speeds things up, provided your grain isn't contaminated.



You shouldn't need to shake after a proper g2g because you already shook them when you did the g2g.

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OfflineWeavieWonder
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Re: Spawning jars early [Re: freespeech]
    #23804997 - 11/05/16 10:18 PM (7 years, 3 months ago)

Quote:

freespeech said:
Do not ever spawn uncolonized grain. If your jars are taking a long time then something is probably wrong. You probably had bacteria in the grain master or introduced it during the g2g. Even so, your best bet is to wait it out and see if they finish colonizing before spawning.

Quote:

WeavieWonder said:
Also, have you shaken your grain at any point from G2G to now?  A good shake a 30 -50% colonization really speeds things up, provided your grain isn't contaminated.



You shouldn't need to shake after a proper g2g because you already shook them when you did the g2g.




Fair point.  Perhaps I am a little stingy with my G2Gs then.

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Offlineafflictis
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Re: Spawning jars early [Re: WeavieWonder]
    #23805209 - 11/06/16 12:36 AM (7 years, 3 months ago)

Yes I am using an incubator. And I did shake one jar 3 to 4 days ago and it hasn't recovered yet so I don't wanna shake the rest.

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Invisibledankington
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Re: Spawning jars early [Re: afflictis]
    #23805245 - 11/06/16 01:13 AM (7 years, 3 months ago)

if it didn't recover, it's because it's too contaminated. A week an a half isn't crazy, but for g2g 50% colonized is a bit low. Your incubator could be more problems than it's worth. Can you just keep the jars at room temperature instead?

If your jars have stalled, and aren't colonizing more, it's because they're contaminated. You'll need to learn to read the characteristics of mycelial growth, and when it's clean, when it's reacting to bacteria, etc. That will just come with practice. Do not spawn jars that aren't finishing colonization. You can case and fruit from the jars, if you want, but they're not worth spawning into tubs.

If you're doing g2g, are you starting with Agar? If you're doing spores to grain, just keep in mind there's often going to be some bacteria here and there, so you'll want to be very critical of how your growth looks. We use agar to try and ensure we have as clean a form of inoculation as possible.

Ultimately, you're going to need to show pictures for any of us to make any judgements. Otherwise it's like trying to teach an old person how to use their computer over the telephone.

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InvisibleTheEaglesGift
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Re: Spawning jars early [Re: dankington]
    #23805364 - 11/06/16 03:49 AM (7 years, 3 months ago)

They're contaminated or they'd be recovering.

Pictures?

I don't know if you used agar to procure a clean culture, but if you don't use agar you should.

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Offlineafflictis
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Re: Spawning jars early [Re: TheEaglesGift]
    #23807629 - 11/06/16 06:08 PM (7 years, 3 months ago)

I'll try to upload a pic of the one that didn't recover. Is it possible that while using my sab I messed the mycelium up. I sprayed walls with soapy water sprayed lysol and I used a spoon that I sterilized with alcohol to get the wbs out. Could all those chemicals damaged it?

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Invisibledankington
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Re: Spawning jars early [Re: afflictis]
    #23807666 - 11/06/16 06:17 PM (7 years, 3 months ago)

Well, the spoon could have been a vector. Alcohol merely sanitizes, it doesn't really sterilize.
More importantly, because we cannot really see your master, was the grain hard to break up? If you needed a spoon to break the spawn up for a g2g, you missed a very important clue. Clean spawn can be broken apart by tapping the jar firmly against your palm. If it's harder than that, it's likely contaminated. To what degree, is hard to determine without pictures.

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Offlineafflictis
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Re: Spawning jars early [Re: dankington]
    #23808012 - 11/06/16 08:35 PM (7 years, 3 months ago)



Master jar. Spawn was easy to break used the spoon cause my hand didn't fit all the way to the bottom and I didn't want to create airflow by shaking jar in sab

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Invisiblefreespeech
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Re: Spawning jars early [Re: afflictis]
    #23808028 - 11/06/16 08:41 PM (7 years, 3 months ago)

Quote:

afflictis said:


Master jar. Spawn was easy to break used the spoon cause my hand didn't fit all the way to the bottom and I didn't want to create airflow by shaking jar in sab



The way to shake a jar is to shake the closed jar outside of the still air box and then put it into the box to do the g2g... This is how virtually all of us do it. Why would you think you have to shake the jar inside the still air box??

You shouldn't be putting your hands inside the grain master jar. That's a problem waiting to happen. IF you need to use a tool in a sterile procedure, which you DON'T for a g2g, then you need to sterilize the tool properly. A simple way to sterilize a tool is to wrap the heatproof tool in foil and pressure cook it, and then place the foil-wrapped sterile tool inside your still air box, and only unwrap the tool immediately before you use it. A more ghetto way would be to flame the shit out of your spoon with a blowtorch until it's red hot and let it cool inside the still air box before using it. But you shouldn't ever do any of this for a simple g2g.

Overall, I'd say the issue is that you did it wrong. Read up on how to do a g2g and try again.

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Offlineafflictis
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Re: Spawning jars early [Re: freespeech]
    #23808248 - 11/06/16 10:41 PM (7 years, 3 months ago)

Quote:

freespeech said:
Quote:

afflictis said:


Master jar. Spawn was easy to break used the spoon cause my hand didn't fit all the way to the bottom and I didn't want to create airflow by shaking jar in sab



The way to shake a jar is to shake the closed jar outside of the still air box and then put it into the box to do the g2g... This is how virtually all of us do it. Why would you think you have to shake the jar inside the still air box??

You shouldn't be putting your hands inside the grain master jar. That's a problem waiting to happen. IF you need to use a tool in a sterile procedure, which you DON'T for a g2g, then you need to sterilize the tool properly. A simple way to sterilize a tool is to wrap the heatproof tool in foil and pressure cook it, and then place the foil-wrapped sterile tool inside your still air box, and only unwrap the tool immediately before you use it. A more ghetto way would be to flame the shit out of your spoon with a blowtorch until it's red hot and let it cool inside the still air box before using it. But you shouldn't ever do any of this for a simple g2g.

Overall, I'd say the issue is that you did it wrong. Read up on how to do a g2g and try again.




None of the other jars show signs of contams though worth taking to monotub when fully colonized

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InvisibleTheEaglesGift
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Re: Spawning jars early [Re: freespeech]
    #23808298 - 11/06/16 11:16 PM (7 years, 3 months ago)

Quote:

If you needed a spoon to break the spawn up for a g2g, you missed a very important clue. Clean spawn can be broken apart by tapping the jar firmly against your palm. If it's harder than that, it's likely contaminated. To what degree, is hard to determine without pictures.



Great point, dankington.

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