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InvisiblePiptheGreAtest
Aspiring Heavyweight
I'm a teapot User Gallery

Registered: 07/18/16
Posts: 464
Taste of Failure×××Advice on colinzied jar creation???
    #23807997 - 11/06/16 08:27 PM (7 years, 2 months ago)

Ok currently I have a monotub that is about 30%. Following that monotub I tried to knock up like 12 other jars in two sets of six one with Ecudor and Albino PE hybrid. AFTEr waiting a week on each set I had no growth. I'm going on day 10 and nothing is set in. I have been sterile wipe everything down PC the jars. I'M even using a still-air box I don't understand where I'm going wrong. I'M using milo with gysum confused thanks 4 advice.


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OfflineThedillestpickle
cultured
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Registered: 02/02/16
Posts: 1,170
Loc: Canada Flag
Last seen: 3 years, 7 months
Re: Taste of Failure×××Advice on colinzied jar creation??? [Re: PiptheGreAtest]
    #23808167 - 11/06/16 09:41 PM (7 years, 2 months ago)

I've bought syringes in the past that were old and all the spores were dead.  Nothing grew in the jars until I reinoculated 2 weeks later with another syringe from a vendor and got things going. 

It's always a good idea to start with agar so you don't rely on syringes.  get a culture going and then you'll always have live mycelium to inoculate with.


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InvisiblePiptheGreAtest
Aspiring Heavyweight
I'm a teapot User Gallery

Registered: 07/18/16
Posts: 464
Re: Taste of Failure×××Advice on colinzied jar creation??? [Re: Thedillestpickle]
    #23808338 - 11/06/16 11:49 PM (7 years, 2 months ago)

So I do agar from spore print correct?


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Offlinewtfcrazymofo
foil hater
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Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 13 hours, 20 minutes
Re: Taste of Failure×××Advice on colinzied jar creation??? [Re: PiptheGreAtest]
    #23808506 - 11/07/16 02:52 AM (7 years, 2 months ago)

spores to agar>agar>grain >mono


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If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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