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OfflinePinPornProducer
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Registered: 08/23/14
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Re: inoculate pure manure and verm without any grains ? [Re: som776]
    #23806766 - 11/06/16 02:12 PM (7 years, 3 months ago)

I....I don't know....what to say...

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OfflineNDStepp84
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Registered: 04/23/15
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Re: inoculate pure manure and verm without any grains ? [Re: PinPornProducer] * 1
    #23807664 - 11/06/16 06:16 PM (7 years, 3 months ago)

Quote:

som776 said:
Thanks Nd step. I have this , do you think this is good . ive had success before with it transfering spores to agar.





Lots of great growers here, including a lot of TC's don't have a flow hood. With a good SAB and lots of practice with proper procedure you can have reliable clean cultures. One drop of spores can do countless grows, you are forcing the culture to grow on a 2d surface where contams can be spotted and clean myc can be transferred away to a new plate where it should grow clean, unlike a liquid culture where you can't tell its clean by looking at it.

I highly recommend building a good SAB like in the first link, 105 quart or better so you have plenty of room to work towards the back without reaching over your work, bigger armholes are better at least 5", bigger armholes create less air currents when you move your arms.


--------------------

"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread

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Offlinesom776
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Registered: 10/16/15
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Re: inoculate pure manure and verm without any grains ? [Re: PinPornProducer]
    #23807786 - 11/06/16 07:00 PM (7 years, 3 months ago)

Thanks nd for the solid advice without acting like a judgemental a hole .

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Offlinesom776
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Re: inoculate pure manure and verm without any grains ? [Re: som776]
    #23860380 - 11/23/16 11:43 AM (7 years, 3 months ago)

What do you guys think of soaking the dry manure in a sort of diluted agar then p.c. before inoculating with l.c. , since some say manure agar for pan species colonize quicker.  Has anyone tried this ??
Thanks.

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