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InvisibleMycolorado
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Re: Monotub help - I've researched all I can! [Re: mushboy]
    #23803314 - 11/05/16 12:48 PM (7 years, 2 months ago)

I live in a high-plains desert and contams are minimal.  If I were growing in Florida, I might have more issues and may need to adjust my procedure accordingly in order to grow contam free.  All situations are unique and the environment can play a big role when it comes to contams.  Something to consider...


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InvisiblemushboyMDiscord
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Re: Monotub help - I've researched all I can! [Re: Mycolorado]
    #23803320 - 11/05/16 12:50 PM (7 years, 2 months ago)

Quote:

NightPuma1 said:
agar is the only chance I have for doing a monotub with any reasonable percentage of chance of it working.




spoken like a pro:thumbup:


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OfflineEdmunter
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Re: Monotub help - I've researched all I can! [Re: NightPuma1]
    #23803418 - 11/05/16 01:28 PM (7 years, 2 months ago)

My advise is to read everything this guy does

https://www.shroomery.org/forums/showflat.php/Number/18431059

and this guy

https://www.shroomery.org/forums/showprofile.php?User=284254

watch these

https://www.shroomery.org/forums/showflat.php/Number/21032261

and use this tek to limit your waste if it goes south

https://www.shroomery.org/forums/showflat.php/Number/21032606

You can also test syringes by making PF cakes first.

Dunking grains is standard practice by the way

and if you want a bit of old school watch these

http://www.mushroomvideos.com/

I prefer the contempory artists but These guys above are my how to manual...
















https://www.shroomery.org/forums/showflat.php/Number/17616982#17616982


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InvisibleInocuole
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Re: Monotub help - I've researched all I can! [Re: mushboy]
    #23803436 - 11/05/16 01:37 PM (7 years, 2 months ago)

Quote:

mushboy said:
some admin/mega poster has a great line.

'forget what you know, all that matters is clean spawn' ....or something.





... You can't just go putting words in people's mouths bro.  That's cron's sig, and it says "It doesn't matter what I think of you.  All that matters is clean spawn"


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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OfflineEdmunter
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Re: Monotub help - I've researched all I can! [Re: Inocuole]
    #23803449 - 11/05/16 01:43 PM (7 years, 2 months ago)

Quote:

Inocuole said:
Quote:

mushboy said:
some admin/mega poster has a great line.

'forget what you know, all that matters is clean spawn' ....or something.





... You can't just go putting words in people's mouths bro.  That's cron's sig, and it says "It doesn't matter what I think of you.  All that matters is clean spawn"



:laugh2:


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InvisiblemushboyMDiscord
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Re: Monotub help - I've researched all I can! [Re: Edmunter]
    #23803459 - 11/05/16 01:45 PM (7 years, 2 months ago)

:wutermelon:


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InvisiblebodhisattaMDiscordReddit
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Re: Monotub help - I've researched all I can! [Re: mushboy]
    #23804617 - 11/05/16 08:05 PM (7 years, 2 months ago)

https://www.shroomery.org/forums/showflat.php/Number/22721954/vc/1#22721954


https://www.shroomery.org/forums/showflat.php/Number/23662427


It you're using spores to grain you shouldn't be doing g2g with those jars ideally.
Spawn jars inoculated with agar are going to be your best bet for G2G jars.

Your problems started well before the substrate

Also you shouldn't be using tons of alcohol and lysol etc.. that just shows lack of understanding sterile technique. Sanitizers wont save you from yourself


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OfflineAlCapone2k
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Re: Monotub help - I've researched all I can! [Re: bodhisatta]
    #23804774 - 11/05/16 09:03 PM (7 years, 2 months ago)

The problem is, that you didn't realize that Grain 2 Grain is not a tek with a guarantee of getting clean spawn. G2G is a tek which needs clean spawn.

The only way to get a clean spawn master is agar. All the well known G2G Teks described here (Spitball Jedi's for example) base on a agar inoculation of the master jar (or at least clean LC/LI).

In most G2G Teks the way how they got their Master isn't decribed, therefore you have the impression that the master could have been made with spore syringes.

But if you search for other teks these guys wrote, you will recognize, that most of them work with agar. So you can be sure, their master jar was made with agar wedges.


Especially your reference to RR. I actually haven't seen or read RR saying that his teks base on spore syringes. The only time he uses a spore syringe in his videos is the one about PF Tek.

Regards :smile:


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


Edited by AlCapone2k (11/05/16 09:05 PM)


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InvisibleTheEaglesGift
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Re: Monotub help - I've researched all I can! [Re: AlCapone2k]
    #23804889 - 11/05/16 09:35 PM (7 years, 2 months ago)

Learn agar.

Also, what are your grain jar lids like? Something that's easy to overlook, but can seriously fuck a grow up. I'm surprised nobody asked already..

And learn agar.


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OfflineWeavieWonder
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Re: Monotub help - I've researched all I can! [Re: AlCapone2k]
    #23804936 - 11/05/16 09:52 PM (7 years, 2 months ago)

If you read Frank's Tek closely, he states that dunking your grains is only a good idea if your spawn is CLEAN.  It is clear that yours is not, sorry man.  A grain jar can fully colonize and then be used to spawn bulk substrate and fully colonize that, and still be contaminated with bacteria.  Bacteria usually leads to mold, typically the green variety.  The mold usually shows up anytime between near colonization and pre first flush. 

    Mushboy is right, we need to know your method of inoculation.  Syringes are usually contaminated.  A dirty MS syringe and or improperly prepared grain can lead to a failure, almost every time.


Edited by WeavieWonder (11/05/16 11:11 PM)


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InvisibleMycolorado
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Re: Monotub help - I've researched all I can! [Re: WeavieWonder]
    #23805159 - 11/05/16 11:46 PM (7 years, 2 months ago)

This is directly from syringe...


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OfflineWeavieWonder
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Re: Monotub help - I've researched all I can! [Re: Mycolorado]
    #23805182 - 11/06/16 12:02 AM (7 years, 2 months ago)

Mucho bacterio.  Might be able to get a clean sample with a couple of transfers.


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InvisibleComebackKid
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Re: Monotub help - I've researched all I can! [Re: WeavieWonder]
    #23805238 - 11/06/16 01:00 AM (7 years, 2 months ago)

Quote:

NightPuma1 said:
Later I smack my fully-colonized WBS jars against a tire to break everything up


These jars are as white and fluffy as I've ever seen in any picture online.




Say no more

https://www.shroomery.org/forums/showflat.php/Number/23662280


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:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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InvisibleTheEaglesGift
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Re: Monotub help - I've researched all I can! [Re: Mycolorado]
    #23805354 - 11/06/16 03:22 AM (7 years, 2 months ago)

Quote:

Mycolorado said:
This is directly from syringe...





Transfer from 11 o clock and 7 o clock. You can clean that up easy.


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InvisibleMycolorado
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Re: Monotub help - I've researched all I can! [Re: TheEaglesGift]
    #23805629 - 11/06/16 08:03 AM (7 years, 2 months ago)

Quote:

TheEaglesGift said:
Quote:

Mycolorado said:
This is directly from syringe...





Transfer from 11 o clock and 7 o clock. You can clean that up easy.



Yeah, I posted the pic as an example for the op.  I've been cleaning cultures since the 90s.


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Offlinespacechildo
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Re: Monotub help - I've researched all I can! [Re: Mycolorado]
    #23805746 - 11/06/16 08:51 AM (7 years, 2 months ago)

you should transfer way sooner,before so much nasty stuff gets a chance to grow.
streaking the spores on the plate is a good way of stretching them out so its even easier to see where its pure cube (or your species) myc and where the nasty stuff grows.


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InvisibleMycolorado
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Re: Monotub help - I've researched all I can! [Re: spacechildo]
    #23805760 - 11/06/16 08:56 AM (7 years, 2 months ago)

These were streaked.  I transferred several days ago.  I posted this as an example for the op to illustrate the point that syringes can have bacteria in them.  I feel like I'm repeating myself.


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InvisiblemushboyMDiscord
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Re: Monotub help - I've researched all I can! [Re: Mycolorado]
    #23805770 - 11/06/16 09:00 AM (7 years, 2 months ago)

:thisfuckinguy:

he was just pointing something out for someone who maybe didnt know streaking.
its a forum ya kno.


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InvisiblebodhisattaMDiscordReddit
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Re: Monotub help - I've researched all I can! [Re: Mycolorado]
    #23805773 - 11/06/16 09:01 AM (7 years, 2 months ago)

Quote:

Mycolorado said:
This is directly from syringe...




They mean streaked like this




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InvisibleMycolorado
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Re: Monotub help - I've researched all I can! [Re: bodhisatta]
    #23805786 - 11/06/16 09:06 AM (7 years, 2 months ago)

Yes, I know.


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stonesun
Mycolorado’s Tamp Fruit Project
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