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OfflineNightPuma1
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Re: Monotub help - I've researched all I can! [Re: mushboy]
    #23803116 - 11/05/16 11:41 AM (7 years, 2 months ago)

"Fucked up jars wont get to 100%(most of the time) so jar prep sounds ok!!"

"its the fucking spawn because the inoculate is DIRTY"

But my jars ARE getting to 100%...I mean I even shake them at 100% sometimes and they fully recover. They smell great with no discoloration. I wait days AFTER they look fully white all over to make sure they're fully colonized on the inside.

I'm not trying to argue I just feel like your two statements above are contrasting.


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InvisiblemushboyMDiscord
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Re: Monotub help - I've researched all I can! [Re: PinPornProducer]
    #23803122 - 11/05/16 11:43 AM (7 years, 2 months ago)

fucked up as in moisture content/exploded grains/stalled growth from bad prep.
your spores are dirty. everything else after that is irrelevant.

:badmanthisfeels:spawn denial strong in this one it is.


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OfflinePinPornProducer
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Re: Monotub help - I've researched all I can! [Re: NightPuma1]
    #23803125 - 11/05/16 11:44 AM (7 years, 2 months ago)

The force is strong with this one...


Fkn beat me to it mush lol


Edited by PinPornProducer (11/05/16 11:45 AM)


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OfflineNightPuma1
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Re: Monotub help - I've researched all I can! [Re: PinPornProducer]
    #23803134 - 11/05/16 11:47 AM (7 years, 2 months ago)

I follow the cleanest inoculation procedure I can find: I use a glovebox with tons of alcohol / aerosol sprayed EVERYWHERE, using only sterile equipment, rubber gloves ect.

People do do this right? All these guides exist for a reason right? I just don't see what I'm doing differently...I follow these procedures to the T being extremely sanitary just as other people do. I wind up with tons of completely white spawn. Someone suggested using agar, and I'm sure that is great, but plenty of guides exist without using agar right? Are you guys saying that all these guides are lying as are the people who use them?

This is just the first time I've ever heard that fully white colonized WBS jars are actually secretly contaminated...why does the GTG transfer even exist then?


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InvisiblemushboyMDiscord
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Re: Monotub help - I've researched all I can! [Re: NightPuma1]
    #23803136 - 11/05/16 11:49 AM (7 years, 2 months ago)

example!!!

jar is 100% looks fine right? it smelled all shroomy and broke up nice..
recovered after a shake in 36hrs. good to go!!!

at day 9 of fruiting it got a nice green spot. it also happened to be from
a syringe knocked into 7quarts of wbs. in 7 shoe boxes. 6 went green.

i havent even thought about green in awhile because i was cleaning up my
spores on agar plates while that freak show grow was happening.

ALSO considering the title of the thread 'ive researched all i can' and
no one has taken a giant shit in here is truly shocking.


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OfflineNightPuma1
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Re: Monotub help - I've researched all I can! [Re: NightPuma1]
    #23803140 - 11/05/16 11:51 AM (7 years, 2 months ago)

I'm fine with admitting that my spawn may be contaminated in some way. I guess my question is why do these guides exist telling you to do it this way then? You guys act like I'm making shit up here, and not following it from some of the most popular and widely used guides there are out there.

I don't get it and I'm not trying to be a dick I'm trying to understand.


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OfflinePinPornProducer
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Re: Monotub help - I've researched all I can! [Re: NightPuma1]
    #23803151 - 11/05/16 11:55 AM (7 years, 2 months ago)

If your shooting spores from a syringe into grain jars, and a tek showed you to this then the person that made that tek is not worth listening to.


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Re: Monotub help - I've researched all I can! [Re: PinPornProducer]
    #23803154 - 11/05/16 11:56 AM (7 years, 2 months ago)

Quote:

NightPuma1 said:
Someone suggested using agar, and I'm sure that is great, but plenty of guides exist without using agar right? Are you guys saying that all these guides are lying as are the people who use them?




the biggest thing to consider is clean spawn. everything after that is up to chance(as in contams either bacterial or more insidious) so you limit the chances.
agar lets you grow out the culture to guarantee it is CLEAN as possible.

people assume the jars are clean because they grew. i get you. i was in the dark
until i learned what bacterial jars were all about. it also explained my HIGH failure rate.

you CAN do MS grows to grains.(use th search feature and search ms grains)

you CAN also have sex with a dirty crackwhore and maybe still be clean.


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OfflineNightPuma1
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Re: Monotub help - I've researched all I can! [Re: PinPornProducer]
    #23803156 - 11/05/16 11:58 AM (7 years, 2 months ago)

My problem occurs when I do GTG transfers as well.


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InvisiblemushboyMDiscord
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Re: Monotub help - I've researched all I can! [Re: NightPuma1]
    #23803158 - 11/05/16 11:59 AM (7 years, 2 months ago)

because the first jar(master jar) is dirty dude!


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OfflineNightPuma1
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Re: Monotub help - I've researched all I can! [Re: mushboy]
    #23803159 - 11/05/16 11:59 AM (7 years, 2 months ago)

So you're saying trash these 20 jars I've made from GTG transfer that all look really good and get into doing agar?


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InvisiblemushboyMDiscord
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Re: Monotub help - I've researched all I can! [Re: mushboy]
    #23803164 - 11/05/16 12:01 PM (7 years, 2 months ago)

yes.


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InvisibleMycolorado
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Re: Monotub help - I've researched all I can! [Re: NightPuma1]
    #23803168 - 11/05/16 12:02 PM (7 years, 2 months ago)

You could transfer a seed to agar and clean it up and then go back to grain.


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Re: Monotub help - I've researched all I can! [Re: mushboy]
    #23803169 - 11/05/16 12:02 PM (7 years, 2 months ago)

OR while you fuck around with the potentially dirty jars...

also do agar work. its really really easy once you get things
going. it just requires patients and lots more reading.

something a lot of people dont seem to want to do. hence hesitation around agar.

also IF I WERE YOU and i wanted to fruit the 20 jars, id do each one in small trays in a sgfc or something.. if you cant get 1/20 ms jars to fruit then you are fucked.


Edited by mushboy (11/05/16 12:09 PM)


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Re: Monotub help - I've researched all I can! [Re: Mycolorado]
    #23803190 - 11/05/16 12:11 PM (7 years, 2 months ago)

Quote:

Mycolorado said:
You could transfer a seed to agar and clean it up and then go back to grain.




that too

oops on the double post.

also @mycolorado try it out!


Edited by mushboy (11/05/16 12:14 PM)


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InvisibleMycolorado
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Re: Monotub help - I've researched all I can! [Re: mushboy]
    #23803212 - 11/05/16 12:17 PM (7 years, 2 months ago)

Will do!


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Offlinespacechildo
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Re: Monotub help - I've researched all I can! [Re: NightPuma1]
    #23803222 - 11/05/16 12:20 PM (7 years, 2 months ago)

Quote:

NightPuma1 said:
My problem occurs when I do GTG transfers as well.




no, your problem is you're dealing with microbes so small you cant see it with the naked eye.
you THINK your spawn is clean, because its all white, but most myc is white, even mold.
so nasty stuff colonizes your grains, then your tubs, and once you fruit your tubs you also fruit your contams making them sporulate and thats when you first notice something's wrong, even tho soemthing has been wrong the entire time.


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Re: Monotub help - I've researched all I can! [Re: spacechildo]
    #23803248 - 11/05/16 12:27 PM (7 years, 2 months ago)

Quote:

spacechildo said:
and once you fruit your tubs you also fruit your contams.



:cookiemonster:


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OfflineNightPuma1
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Re: Monotub help - I've researched all I can! [Re: mushboy]
    #23803261 - 11/05/16 12:31 PM (7 years, 2 months ago)

So basically what I'm hearing is fuck all these guides all these huge names swear by (RR, Frank ect.) and agar is the only chance I have for doing a monotub with any reasonable percentage of chance of it working. I mean if that's what you're saying I guess I have no choice - seems fucking odd to me this isn't talked about a WHOLE LOT more.


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Re: Monotub help - I've researched all I can! [Re: NightPuma1]
    #23803280 - 11/05/16 12:37 PM (7 years, 2 months ago)

this is talked about all the fucking time.

and even in franks tek you follow it says...

Quote:

A properly pasteurized substrate and clean grain spawn are as contam resistant as it gets, so this dunk can be done with minimal precautions- as long as your grains are fully colonized and substrate properly pasteurized.




clean grain spawn means your inoculate(either agar or LI/LC is clean)

on a side note.. my first grows were in a BRAND NEW house. like custom build
brand fucking new paint still wet when moved in new. i barely had contams and
was doing spores to brf and to grains. few issues. fast foward 10 years and
where i live now is a rowhouse built in the 30s. 90% failure until i started
cleaning up my spores on agar first. i havent had anything in months except mushrooms. go figure.

read franks ENTIRE posts.. like the whole fucking thread all 30+
pages of each one. as with most...not all, but most teks, the poster will
update and often times contradict there own teks.

some admin/mega poster has a great line.

'forget what you know, all that matters is clean spawn' ....or something.


Edited by mushboy (11/05/16 12:48 PM)


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