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OfflineRainydays
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Tryptic Soy Agar Pre-poured Petri Dishes
    #23802370 - 11/05/16 02:11 AM (7 years, 2 months ago)

I am curious if pre-poured petri dishes are a reliable and consistent way to do agar. Is there anything I should watch out for or do to the dishes to make them effective? I plan on doing this with PE, B+,ECU, and BUR; but mainly I plan on doing it with PE once I get familiar with agar.


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Offlinetump
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Re: Tryptic Soy Agar Pre-poured Petri Dishes [Re: Rainydays]
    #23802374 - 11/05/16 02:12 AM (7 years, 2 months ago)

Buying them is costly but reliable as any other pre made lab stuff.


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Offlinewtfcrazymofo
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Re: Tryptic Soy Agar Pre-poured Petri Dishes [Re: Rainydays]
    #23802378 - 11/05/16 02:17 AM (7 years, 2 months ago)

Pre pour is for aaa.... making money try this bro:

https://www.shroomery.org/forums/showflat.php/Number/19208976


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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Invisibledankington
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Re: Tryptic Soy Agar Pre-poured Petri Dishes [Re: Rainydays]
    #23802379 - 11/05/16 02:17 AM (7 years, 2 months ago)

well, to grow all that, you're probably going to go through anywhere from 20-30 plates (very conservatively, assuming 1-2 grows of each), or as many as 150 or more. So, cost could be an issue at some point. You'll need a way to keep cultures going, like master slants and such.

So, I really suggest you start trying to pour some plates, or getting into pre-pour agar. Do you have an SAB and everything? Did you mean tryptic like plates with three divisions? Those don't give as much room for growth, but I can see the advantages. I prefer letting the myc grow a bit more than that though.


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OfflineRainydays
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Re: Tryptic Soy Agar Pre-poured Petri Dishes [Re: dankington]
    #23802398 - 11/05/16 02:40 AM (7 years, 2 months ago)

Wow thanks for the speedy replies. Thanks for the link mofo.
Dan I am in the process of a buying the equipment I am going to do a flow hood instead, is a SAB better? I am wanting to get stuff to simplify things at the beginning of the learning process so i don't have to many variables for error.


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OfflinePsilosopherr
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Re: Tryptic Soy Agar Pre-poured Petri Dishes [Re: Rainydays]
    #23802416 - 11/05/16 03:12 AM (7 years, 2 months ago)

Nothing wrong with SAB's, certainly not better though.

If you're just learning I wouldn't be sinking money into a flowhood. I'm still debating whether I want/need one years into it.


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Offlinewtfcrazymofo
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Re: Tryptic Soy Agar Pre-poured Petri Dishes [Re: Rainydays]
    #23802419 - 11/05/16 03:19 AM (7 years, 2 months ago)

I just whipped up my hood, and I miss my sab, so simple.  With a hood you got to be more clean it seems(iso gloves at least).  I just whipped up some p plates and I plan to use my sab again soon.


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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OfflineRainydays
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Registered: 11/04/16
Posts: 15
Last seen: 7 years, 30 days
Re: Tryptic Soy Agar Pre-poured Petri Dishes [Re: wtfcrazymofo]
    #23802429 - 11/05/16 03:38 AM (7 years, 2 months ago)

I will do a coin toss on my next paycheck to see if I make a SAB or flowhood. My flow hood idea would have a 2'x2'x3' plexiglass front with 32 cfm of squirrel fans on a 8.8"x8.8" hepa filter for 120$ total of supplies.


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Offlinewtfcrazymofo
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Re: Tryptic Soy Agar Pre-poured Petri Dishes [Re: Rainydays]
    #23802431 - 11/05/16 03:40 AM (7 years, 2 months ago)

You'd better just make a sab.


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




Extras: Filter Print Post Top
OfflineRainydays
Stranger
I'm a teapot
Registered: 11/04/16
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Last seen: 7 years, 30 days
Re: Tryptic Soy Agar Pre-poured Petri Dishes [Re: wtfcrazymofo]
    #23802435 - 11/05/16 03:46 AM (7 years, 2 months ago)

All logical reasoning points to that option, I guess it is a better idea anyways the fridge needs stocked up some more anyways.


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Offlinewtfcrazymofo
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Re: Tryptic Soy Agar Pre-poured Petri Dishes [Re: Rainydays]
    #23802446 - 11/05/16 03:56 AM (7 years, 2 months ago)

Quote:

Rainydays said:
I will do a coin toss on my next paycheck to see if I make a SAB or flowhood. My flow hood idea would have a 2'x2'x3' plexiglass front with 32 cfm of squirrel fans on a 8.8"x8.8" hepa filter for 120$ total of supplies.




Never leave a life's decision to a dirty coin toss.  The flow hood idea you got is wrong.


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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OfflineRainydays
Stranger
I'm a teapot
Registered: 11/04/16
Posts: 15
Last seen: 7 years, 30 days
Re: Tryptic Soy Agar Pre-poured Petri Dishes [Re: wtfcrazymofo]
    #23802450 - 11/05/16 04:00 AM (7 years, 2 months ago)

That tends to be how I live is by a coin toss, quite literally. How far in the wrong is my flow hood idea?


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Invisibleblindingleaf
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Re: Tryptic Soy Agar Pre-poured Petri Dishes [Re: Rainydays]
    #23802451 - 11/05/16 04:01 AM (7 years, 2 months ago)

its like ummmmm 200cfm+ wrong


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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OfflineRainydays
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I'm a teapot
Registered: 11/04/16
Posts: 15
Last seen: 7 years, 30 days
Re: Tryptic Soy Agar Pre-poured Petri Dishes [Re: blindingleaf]
    #23802456 - 11/05/16 04:04 AM (7 years, 2 months ago)

lol, ok now I see. That is about 50-60$ more onto that price I had stated above. I just figured if it was more than 2x the displacement of the room of the hood it would be good but I'm wrong.


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OfflineGreg
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Re: Tryptic Soy Agar Pre-poured Petri Dishes [Re: Rainydays]
    #23802969 - 11/05/16 10:42 AM (7 years, 2 months ago)

Yeah man just use a SAB.
I say this a lot but I've only had 3 contams in the 300+ plates I've poured in my SAB.
(That's before using the plates, all three were single bacteria colonies.)

Long ago in the dark ages I bought a pack of pre-poured dishes and half of them came either broken or contamed.


Edited by Greg (11/05/16 10:47 AM)


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