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candela
Stranger


Registered: 09/04/15
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Coir/Verm to Agar experiment
#23801993 - 11/04/16 10:05 PM (7 years, 2 months ago) |
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Yes, another experimental question for the board.
A lot of us on here swear the coir and verm is very contam resistant. Many board members have left there coir and verm in buckets without any contaminants settling in. So, my question is, especially for those who have been around along time, has anyone on here actually taken coir or verm (pasteurized/unpasteurized) and placed it onto an agar plate.
Half of the members on here will state the contamination only comes from bad spawn. So, it will be very interesting to see if there is hidden contams in coir and verm by adding nutes into it, thru agar plates, to see if it triggers any hidden contams in cv.
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: Coir/Verm to Agar experiment [Re: candela]
#23802007 - 11/04/16 10:12 PM (7 years, 2 months ago) |
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Ya a dude did it about a month or two ago i forget who rooster cogburn i think it was
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Mushierage
SWIM Sinker



Registered: 06/29/16
Posts: 1,094
Last seen: 7 years, 1 month
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Re: Coir/Verm to Agar experiment [Re: Boogieman47]
#23802459 - 11/05/16 04:08 AM (7 years, 2 months ago) |
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I don't see any reason why you couldn't do this, as long as you plan on doing at least a couple transfers down the line. But also really don't see any reason to ever do this unless you forgot to do it with some extra grain spawn you might have.
Why start out with more contaminants than you have to is my philosophy. You could just wait for a MS fruit, and clone that several times over too if you are using MS. If not, just pop a grain kernel on a few agar plates and transfer away from any contams.
-------------------- Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup. OR... You should be cultivating reptiles and fish, not mushrooms.
  
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tump
ban the undead


Registered: 03/17/16
Posts: 2,383
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Re: Coir/Verm to Agar experiment [Re: Mushierage]
#23802487 - 11/05/16 04:48 AM (7 years, 2 months ago) |
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I think that guy fould out that other molds grow out on the coir agar. He never did the verm agar
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littlespider
spider


Registered: 05/21/14
Posts: 496
Loc: UK
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Re: Coir/Verm to Agar experiment [Re: Mushierage]
#23802490 - 11/05/16 04:53 AM (7 years, 2 months ago) |
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Quote:
Mushierage said: I don't see any reason why you couldn't do this, as long as you plan on doing at least a couple transfers down the line. But also really don't see any reason to ever do this unless you forgot to do it with some extra grain spawn you might have.
Why start out with more contaminants than you have to is my philosophy. You could just wait for a MS fruit, and clone that several times over too if you are using MS. If not, just pop a grain kernel on a few agar plates and transfer away from any contams.
I was wondering why i havent seen people put grain directly into a colonised agar tub
-------------------- remember what the dormouse said
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Coir/Verm to Agar experiment [Re: candela] 1
#23802892 - 11/05/16 10:08 AM (7 years, 2 months ago) |
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Quote:
candela said: Yes, another experimental question for the board.
A lot of us on here swear the coir and verm is very contam resistant. Many board members have left there coir and verm in buckets without any contaminants settling in. So, my question is, especially for those who have been around along time, has anyone on here actually taken coir or verm (pasteurized/unpasteurized) and placed it onto an agar plate.
Half of the members on here will state the contamination only comes from bad spawn. So, it will be very interesting to see if there is hidden contams in coir and verm by adding nutes into it, thru agar plates, to see if it triggers any hidden contams in cv.
then it would be the agar allowing contams to grow, not the coir. you cant put dirty stuff on agar and expect it to grow clean. but you can leave dirty coir on its own and it wont grow any nasties.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Coir/Verm to Agar experiment [Re: spacechildo] 1
#23803012 - 11/05/16 10:58 AM (7 years, 2 months ago) |
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Quote:
spacechildo said:
Quote:
candela said: Yes, another experimental question for the board.
A lot of us on here swear the coir and verm is very contam resistant. Many board members have left there coir and verm in buckets without any contaminants settling in. So, my question is, especially for those who have been around along time, has anyone on here actually taken coir or verm (pasteurized/unpasteurized) and placed it onto an agar plate.
Half of the members on here will state the contamination only comes from bad spawn. So, it will be very interesting to see if there is hidden contams in coir and verm by adding nutes into it, thru agar plates, to see if it triggers any hidden contams in cv.
then it would be the agar allowing contams to grow, not the coir. you cant put dirty stuff on agar and expect it to grow clean. but you can leave dirty coir on its own and it wont grow any nasties.
So painfully obvious im sad it had to be explained
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candela
Stranger


Registered: 09/04/15
Posts: 281
Last seen: 2 years, 6 months
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Re: Coir/Verm to Agar experiment [Re: bodhisatta]
#23803138 - 11/05/16 11:49 AM (7 years, 2 months ago) |
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I understand what you saying spacechildo, So, it is the introduction of nutes to coir/ verm the cause of hidden bacteria spreading, and thats when we see contams pop up?
Edited by candela (11/05/16 11:54 AM)
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: Coir/Verm to Agar experiment [Re: candela]
#23803145 - 11/05/16 11:53 AM (7 years, 2 months ago) |
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Wrong spot haha
Edited by Boogieman47 (11/05/16 11:54 AM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Coir/Verm to Agar experiment [Re: Boogieman47]
#23803150 - 11/05/16 11:55 AM (7 years, 2 months ago) |
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Trich spores are not germinating on coir Trich spores are not germinating on clean spawn. Trich on spawn will happily continue to grow on coir Coir can have trich spores from the air if you put it on agar it may grow
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Coir/Verm to Agar experiment [Re: candela]
#23803172 - 11/05/16 12:03 PM (7 years, 2 months ago) |
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Quote:
candela said: I understand what you saying spacechildo, So, it is the introduction of nutes to coir/ verm the cause of hidden bacteria spreading, and thats when we see contams pop up?
not quite sure what you're saying there, but the reason coir onto agar grow nasties is because the coir has been in contact with open air and therefore gotten nasty stuff from the air on it.
the reason coir grows contams is because your spawn is not clean, if that's what you're saying, yes. nasty stuff already germinated on your grains and colonizes coir just like clean myc growth does.
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