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OfflineAnEdgyUserName
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Failed Colonization of PF/BRF Jars
    #23799800 - 11/04/16 06:00 AM (7 years, 2 months ago)

After using a grow kit to learn some fundamentals about mushroom growing i decided to try the PF Tek. The specific tek i followed was a mix between the Pf Tek v2 and the pf tek done right. I made some spore syringes from the grow kit that I previously mentioned and proceeded to inoculate around 10 jars. I had let the jard sit around a week before i got to inoculation and one was already fully colonized with i dont know what. So it was thrown out. The other 9 were all inoculated with 1cc per jar and left to sit with a 12/12 light schedule. After 2 weeks i saw no signs of life and decided to open one up because i read posts about people having near fully colonized jars after a while but not seeing any exterior signs of life. Nope, the mixture came out as a cake but when I opened it up there was no mycelium at all. Im going to give it another shot but i am wondering what the most likely cause of failure was here. Thanks for any advice.


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Offlinespacechildo
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Re: Failed Colonization of PF/BRF Jars [Re: AnEdgyUserName]
    #23799865 - 11/04/16 06:58 AM (7 years, 2 months ago)

if an un-inoculated jar had growth you can't have sterilized it properly.

www.mushroomvideos.com video 1-4 is the pf-tek you want.


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OfflineAnEdgyUserName
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Re: Failed Colonization of PF/BRF Jars [Re: spacechildo]
    #23800646 - 11/04/16 01:15 PM (7 years, 2 months ago)

Yeah I figured i didnt do proper sterilization when that happened but what about the rest of the jars? What might have caused those to fail?


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Offlinespacechildo
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Re: Failed Colonization of PF/BRF Jars [Re: AnEdgyUserName]
    #23800704 - 11/04/16 01:42 PM (7 years, 2 months ago)

if 1 jar was so poorly sterilized you could see nasty stuff growing why would the others be any better?
just start over, steam 90mins at least with a tight fitting lid.


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OfflineAnEdgyUserName
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Re: Failed Colonization of PF/BRF Jars [Re: spacechildo]
    #23806840 - 11/06/16 02:36 PM (7 years, 2 months ago)

Quote:

spacechildo said:
if 1 jar was so poorly sterilized you could see nasty stuff growing why would the others be any better?
just start over, steam 90mins at least with a tight fitting lid.




Alright will do, I think I did a loose lid last time during the sterilization but ill try tight this time thanks!


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OfflineFreemind777
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Re: Failed Colonization of PF/BRF Jars [Re: AnEdgyUserName]
    #23807242 - 11/06/16 04:07 PM (7 years, 2 months ago)

Bad spores?


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OfflineAnEdgyUserName
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Re: Failed Colonization of PF/BRF Jars [Re: Freemind777]
    #23807397 - 11/06/16 05:06 PM (7 years, 2 months ago)

Quote:

Freemind777 said:
Bad spores?




I did the syringe myself so that is a possibility. I have some properly made syringes I ordered from one of the sponsors im going to use next batch to eliminate that variable.


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OfflineAnEdgyUserName
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Re: Failed Colonization of PF/BRF Jars [Re: AnEdgyUserName]
    #23825920 - 11/12/16 01:19 PM (7 years, 2 months ago)

UPDATE: after about 3 weeks since inoculation and no signs of life this morning 5 out of the 8 jars had mycelium. The other 3 had some white stuff growing that was too small for me to confirm it was mycelium so ill leave those out. This raises more questions than a failed batch wouldve posed so does anyone have any insight on why there was such a delay for growth? thanks.


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Re: Failed Colonization of PF/BRF Jars [Re: AnEdgyUserName]
    #23825937 - 11/12/16 01:27 PM (7 years, 2 months ago)

If bacteria was present in one jar for certain, then we can assume it was present in all the others, but possibly to a lesser degree. That can definitely slow the mycelium down, but can be overcome, which is what I would guess happened with the jars that now show growth. They're more than likely still bacterial, but you should be able to fruit them and get a harvest out of them in spite of that.


--------------------
One goes into an experiment knowing one might fail.
But one does not undertake an experiment knowing one HAS failed.


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OfflineAnEdgyUserName
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Re: Failed Colonization of PF/BRF Jars [Re: r.lutece]
    #23825941 - 11/12/16 01:29 PM (7 years, 2 months ago)

Quote:

r.lutece said:
If bacteria was present in one jar for certain, then we can assume it was present in all the others, but possibly to a lesser degree. That can definitely slow the mycelium down, but can be overcome, which is what I would guess happened with the jars that now show growth. They're more than likely still bacterial, but you should be able to fruit them and get a harvest out of them in spite of that.



Ah ok thankyou, what would you say is the chance these are the mushrooms I intend to grow vs the mycelium that grew due to bad sterilization before? The mycelium does appear to be forming under the inoculation holes but could this still be the bad mycelium?


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Invisibler.lutece
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Re: Failed Colonization of PF/BRF Jars [Re: AnEdgyUserName] * 1
    #23825950 - 11/12/16 01:34 PM (7 years, 2 months ago)

I don't completely understand the question. Are you asking if there's a chance that the growth you see is actually contamination? I wouldn't think so, particularly if it looks pretty consistent across those five jars you inoculated. Are you able to post a picture? It's always easier to provide advice when we have a picture to work off of.


--------------------
One goes into an experiment knowing one might fail.
But one does not undertake an experiment knowing one HAS failed.


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OfflineAnEdgyUserName
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Re: Failed Colonization of PF/BRF Jars [Re: r.lutece]
    #23825992 - 11/12/16 01:52 PM (7 years, 2 months ago)



The mycelium is still very small and only appears in a few spots on each jar. Not an even 4 growths, some jars only have 1.


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Offlinespacechildo
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Re: Failed Colonization of PF/BRF Jars [Re: AnEdgyUserName]
    #23826115 - 11/12/16 02:49 PM (7 years, 2 months ago)

looks OK so far, now just leave them alone.
start a new batch so you dont mess too much with these.
then repeat.


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OfflineKenetic
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Re: Failed Colonization of PF/BRF Jars [Re: spacechildo]
    #23826912 - 11/12/16 07:04 PM (7 years, 2 months ago)

:whathesaid:
wash, rinse, repeat. 


In two months you'll be so busy you'll forget all about staying patient.  Then you'll just have to worry about keeping up.


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OfflineAnEdgyUserName
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Re: Failed Colonization of PF/BRF Jars [Re: AnEdgyUserName]
    #23828782 - 11/13/16 11:30 AM (7 years, 2 months ago)

Thanks for all the help guys. Ill probably update the thread with the yields whenever that happens but yeah ive already started a new batch and those have been properly sterilized, or so I think. Thanks again!


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OfflineAnEdgyUserName
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Re: Failed Colonization of PF/BRF Jars [Re: AnEdgyUserName]
    #23884533 - 12/01/16 01:38 PM (7 years, 1 month ago)

Well 3 of the jars remain from the original 10. 7 have been lost to mold and of the 3, one jar seems to be fully colonized but the mycelium doesnt seem strong enough for a birth, and it looks like I should wait another week. But, fairly large primodoria have been popping up and I am not too sure what to do. I found some threads on other posts about what to do in this scenario, but the primodoria dont seem as large. Any advice would be appreciated, thanks.



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OfflineEldarFungi
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Re: Failed Colonization of PF/BRF Jars [Re: AnEdgyUserName]
    #23884730 - 12/01/16 02:47 PM (7 years, 1 month ago)

Go ahead and make a fruiting chamber for the two cakes that already have pins. Look up a shot gun fruiting chamber, they are really simple. Fill it with perlite and allow airflow from the bottom. Soak those two cakes for a few hours then sprinkle some vermiculite on them to keep their surface humidity up and throw em' in there on some foil.


--------------------
He remained a learner and explorer, not a warrior; and though in time he could have forged weapons that in his own world would have had power enough to become the matter of great tales and be worth a king’s ransom, he knew that in Faery they would have been of small account. So among all the things that he made it is not remembered that he ever forged a sword or a spear or an arrow-head.
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OfflineAnEdgyUserName
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Re: Failed Colonization of PF/BRF Jars [Re: EldarFungi]
    #23885108 - 12/01/16 04:36 PM (7 years, 1 month ago)

Quote:

EldarFungi said:
Go ahead and make a fruiting chamber for the two cakes that already have pins. Look up a shot gun fruiting chamber, they are really simple. Fill it with perlite and allow airflow from the bottom. Soak those two cakes for a few hours then sprinkle some vermiculite on them to keep their surface humidity up and throw em' in there on some foil.




So you think that they are safe to birth even though the mycelium looks a little thin in some areas? Do I risk the cakes breaking apart or should I be good?


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Re: Failed Colonization of PF/BRF Jars [Re: AnEdgyUserName]
    #23885111 - 12/01/16 04:36 PM (7 years, 1 month ago)

You are good


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I'm tired do me a favor


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