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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 24 days
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Re: inoculate pure manure and verm without any grains ? [Re: dankington]
#23805743 - 11/06/16 08:49 AM (7 years, 2 months ago) |
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I think it's safe to that everything this guy has going as of right now is junk and needs to go in the trash. Needs to study some shit for another few months and start over fresh. Definately starting with agar...
Edited by PinPornProducer (11/06/16 08:52 AM)
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som776
Stranger

Registered: 10/16/15
Posts: 61
Last seen: 7 years, 17 days
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Re: inoculate pure manure and verm without any grains ? [Re: som776]
#23805847 - 11/06/16 09:30 AM (7 years, 2 months ago) |
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Can i do transfers ? They are thick because i had them on the stir plate for the last week . I have many agar jars , but no spores at this time .
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: inoculate pure manure and verm without any grains ? [Re: som776]
#23805853 - 11/06/16 09:32 AM (7 years, 2 months ago) |
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Taking transfers from those is the last thing I would do
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som776
Stranger

Registered: 10/16/15
Posts: 61
Last seen: 7 years, 17 days
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Re: inoculate pure manure and verm without any grains ? [Re: som776]
#23805854 - 11/06/16 09:33 AM (7 years, 2 months ago) |
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 24 days
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Re: inoculate pure manure and verm without any grains ? [Re: som776]
#23805855 - 11/06/16 09:33 AM (7 years, 2 months ago) |
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What's there to transfer? Dude it's all toast, suck it up and start over.
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som776
Stranger

Registered: 10/16/15
Posts: 61
Last seen: 7 years, 17 days
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Re: inoculate pure manure and verm without any grains ? [Re: som776]
#23805866 - 11/06/16 09:35 AM (7 years, 2 months ago) |
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What would you do . how do i keep the spores perfectly clean , i dont have a flow hood but i have a glove box . The cultures are made from agar broth .
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: inoculate pure manure and verm without any grains ? [Re: som776]
#23805873 - 11/06/16 09:38 AM (7 years, 2 months ago) |
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Spores are not 100% clean to begin with. Spores go to agar then get cleaned up.
You want a still air box. Not a glove box
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: inoculate pure manure and verm without any grains ? [Re: som776]
#23805876 - 11/06/16 09:39 AM (7 years, 2 months ago) |
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buy/trade a spore print, do pastywhytes easy agar tek, post pics to get best possible help, get great results!
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som776
Stranger

Registered: 10/16/15
Posts: 61
Last seen: 7 years, 17 days
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Re: inoculate pure manure and verm without any grains ? [Re: spacechildo]
#23805894 - 11/06/16 09:42 AM (7 years, 2 months ago) |
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Would you buy spore prints or spore solution ?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: inoculate pure manure and verm without any grains ? [Re: som776]
#23805898 - 11/06/16 09:43 AM (7 years, 2 months ago) |
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They're the same thing
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som776
Stranger

Registered: 10/16/15
Posts: 61
Last seen: 7 years, 17 days
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Re: inoculate pure manure and verm without any grains ? [Re: som776]
#23805907 - 11/06/16 09:47 AM (7 years, 2 months ago) |
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So if i buy spore solution i can put it intobagar. Should i make the agar soft ?
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 24 days
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Re: inoculate pure manure and verm without any grains ? [Re: som776] 1
#23805947 - 11/06/16 10:01 AM (7 years, 2 months ago) |
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Quote:
som776 said:

These are not good for agar plates if that's what they are. To tall, you want short 1/2 pints or dishes. You really don't want to be sticking the entire scalpel and your finger tips into the plates.
Run some plates, clean the culture up.
Put some clean culture on slants for future use since its already cleaned up so you don't have to start over.

Wedges to grains


Get clean master jars

G2G

Spawn to tubs

Get fruits

Edited by PinPornProducer (11/06/16 10:07 AM)
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som776
Stranger

Registered: 10/16/15
Posts: 61
Last seen: 7 years, 17 days
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Re: inoculate pure manure and verm without any grains ? [Re: PinPornProducer]
#23805995 - 11/06/16 10:20 AM (7 years, 2 months ago) |
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Ok . thanks alot , appreciate the help .
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Raven44
Entry not permitted to muggles



Registered: 12/07/13
Posts: 1,970
Loc: My sovereign reality bubble
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Re: inoculate pure manure and verm without any grains ? [Re: som776]
#23806004 - 11/06/16 10:23 AM (7 years, 2 months ago) |
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Taking spores and making LC is never a good idea. It can work, but don't do it
People.usually use clean mycelium from a petrI for LC.
Look up the "Ll" tek
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som776
Stranger

Registered: 10/16/15
Posts: 61
Last seen: 7 years, 17 days
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Re: inoculate pure manure and verm without any grains ? [Re: som776]
#23806226 - 11/06/16 11:33 AM (7 years, 2 months ago) |
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I do have a few hudred of these pp5 plastic pint size containers . would you recommend me do agar in this ?
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: inoculate pure manure and verm without any grains ? [Re: som776]
#23806429 - 11/06/16 12:38 PM (7 years, 2 months ago) |
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flip them sideways while taking transfers, tall walls can make it harder than it need to be. with a good sterile technique there's no problem.
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som776
Stranger

Registered: 10/16/15
Posts: 61
Last seen: 7 years, 17 days
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Re: inoculate pure manure and verm without any grains ? [Re: som776]
#23806526 - 11/06/16 12:59 PM (7 years, 2 months ago) |
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Thanks space . i have a few dozen liq cultures , some have sediment at the bottom and on the glass walls, is this carmelization? Is it still ok to use ?
Thanks guys .  Im going to buy some more agar mix with spore syringes. Is there anything else you would recommend me buy to make good clean myc , besided a flowhood , dnt have that scratch yet . should of opted into that first but i understand its still very possible without
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NDStepp84
Stellar nuclear waste


Registered: 04/23/15
Posts: 4,956
Last seen: 1 month, 4 days
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Re: inoculate pure manure and verm without any grains ? [Re: som776] 2
#23806568 - 11/06/16 01:12 PM (7 years, 2 months ago) |
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--------------------
"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do. -Robert A. Heinlein
Links and teks ND's grow log and discussion Plant thread
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freespeech
disciple



Registered: 12/12/08
Posts: 1,745
Loc: PNW
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Re: inoculate pure manure and verm without any grains ? [Re: som776]
#23806752 - 11/06/16 02:08 PM (7 years, 2 months ago) |
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Quote:
som776 said:

Holy shit I'm dying
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som776
Stranger

Registered: 10/16/15
Posts: 61
Last seen: 7 years, 17 days
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Re: inoculate pure manure and verm without any grains ? [Re: som776]
#23806757 - 11/06/16 02:09 PM (7 years, 2 months ago) |
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Thanks Nd step. I have this , do you think this is good . ive had success before with it transfering spores to agar.
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