Home | Community | Message Board

NorthSpore.com BOOMR Bag!
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: MagicBag.co All-In-One Bags That Don't Suck   Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Injection Grain Bag

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineCorbin19
Stranger in a Strange Land
Male
Registered: 09/06/16
Posts: 11
Loc: WA
Last seen: 7 years, 3 months
Rye jars contamination a few days after shaking?
    #23798476 - 11/03/16 05:41 PM (7 years, 3 months ago)


So I followed Magash's very simple grain tek on here for rye jars. Soaked for 24 hours, boiled 45 min, and pressure cooked at 15 psi for an hour.  Everything has been going awesome. I shook the jars about 3 days ago.  Today I noticed some yellowish spots/dots growing on some of the uncolonized rye. Any idea what this is? How fucked am I on a scale of 1 to dick in the ass?


--------------------
Boom.

Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Rye jars contamination a few days after shaking? [Re: Corbin19]
    #23798494 - 11/03/16 05:46 PM (7 years, 3 months ago)

:horrified:

Yeah extremely fucked. You have lots of bacteria, and a slime mold coming in on the uncolonized grains.. what is your filter type?

Also I always PC for 2 hours to kill off lots of the endospore producing bacteria.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.

Extras: Filter Print Post Top
OfflineCorbin19
Stranger in a Strange Land
Male
Registered: 09/06/16
Posts: 11
Loc: WA
Last seen: 7 years, 3 months
Re: Rye jars contamination a few days after shaking? [Re: Mad Season]
    #23798517 - 11/03/16 05:54 PM (7 years, 3 months ago)

Bummer...  Looks like it's time for round two. This is my first time doing rye.  I've done brf a bunch with no contam issues and even done wood and wheat for two species and no problem.  I've started spawning to bulk in a greenhouse. Doing that with BRF isn't super ideal. What do you mean by filter system? Pretty much the only filter I have is the tyvek on top and a bandaid covering the needle hole. Will definitely do 2hrs next time.


--------------------
Boom.

Extras: Filter Print Post Top
OfflinePsilosopherr
A psilly goose
Other User Gallery


Registered: 02/15/12
Posts: 12,278
Last seen: 2 months, 10 days
Re: Rye jars contamination a few days after shaking? [Re: Mad Season]
    #23798524 - 11/03/16 05:57 PM (7 years, 3 months ago)

I'd agree, increase PC times. at least 90 minutes, I too do 2 hours every time these days.

How'd you knock em up?

Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Rye jars contamination a few days after shaking? [Re: Corbin19]
    #23798525 - 11/03/16 05:57 PM (7 years, 3 months ago)

Slime mold spores are small, and can get into filters who have large pore sizes. Tyvek after a few runs has big enough pore sizes for the spores to fit through. So maybe you should think about a more permanent filter type, like SFDs :smile:


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.

Extras: Filter Print Post Top
OfflineCorbin19
Stranger in a Strange Land
Male
Registered: 09/06/16
Posts: 11
Loc: WA
Last seen: 7 years, 3 months
Re: Rye jars contamination a few days after shaking? [Re: Psilosopherr]
    #23798535 - 11/03/16 06:00 PM (7 years, 3 months ago)

I use new sterile needles every time and make sure I maintain a good sterile field.  I don't use a flow hood or anything.  Just needle thru the tyvek and Bandaid the hole. It must be endospores I think.  Gotta be more meticulous when I am prepping the rye huh?


--------------------
Boom.

Extras: Filter Print Post Top
OfflineCorbin19
Stranger in a Strange Land
Male
Registered: 09/06/16
Posts: 11
Loc: WA
Last seen: 7 years, 3 months
Re: Rye jars contamination a few days after shaking? [Re: Mad Season]
    #23798552 - 11/03/16 06:06 PM (7 years, 3 months ago)

What's an SFD filter? Sorry if thats a newb question.


--------------------
Boom.

Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Rye jars contamination a few days after shaking? [Re: Corbin19]
    #23798553 - 11/03/16 06:06 PM (7 years, 3 months ago)

Well slime mold doesn't just pop up out of nowhere. It needs the spores to get in somehow, and since 130F for 30 minutes kills it, the PC would have easily killed any in there, which means the spores came in post-pc.

Bandaids really don't stop anything tbh, and inoculating through your filter is a good way to expose it. The slime mold probably just slipped right in right when you shook it. This is why you need a better filter system dude.

The bacteria could have been from an insufficient PC cycle, or it could have ridden in when you shook. Either way you got a couple things to improve upon.

Might I also recommend a still air box and agar.

SFD means synthetic filter disc, it can be found quite easily from a few of the sponsors.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.

Extras: Filter Print Post Top
OfflinePsilosopherr
A psilly goose
Other User Gallery


Registered: 02/15/12
Posts: 12,278
Last seen: 2 months, 10 days
Re: Rye jars contamination a few days after shaking? [Re: Corbin19]
    #23798564 - 11/03/16 06:10 PM (7 years, 3 months ago)

So just spores? Spore syringes aren't sterile so its always iffy.

Correct me if I'm wrong but its entirely possible that you did everything right and got bad luck, might as well try to improve your method anyway though.

Also, its easy to just open the jar briefly and squirt your syringe in that way, so you don't have to push it through the filter which is risky. Just don't let anything but the flame sterilized needle hover above the open jar.

Extras: Filter Print Post Top
OfflineCorbin19
Stranger in a Strange Land
Male
Registered: 09/06/16
Posts: 11
Loc: WA
Last seen: 7 years, 3 months
Re: Rye jars contamination a few days after shaking? [Re: Mad Season]
    #23798570 - 11/03/16 06:11 PM (7 years, 3 months ago)

Werd. For sure need improvement.  Thanks for the help man.


--------------------
Boom.

Extras: Filter Print Post Top
OfflineCorbin19
Stranger in a Strange Land
Male
Registered: 09/06/16
Posts: 11
Loc: WA
Last seen: 7 years, 3 months
Re: Rye jars contamination a few days after shaking? [Re: Psilosopherr]
    #23798581 - 11/03/16 06:15 PM (7 years, 3 months ago)

Isn't opening the jars and exposing them to The environment more risky due to more surface area?


--------------------
Boom.

Extras: Filter Print Post Top
OfflinePsilosopherr
A psilly goose
Other User Gallery


Registered: 02/15/12
Posts: 12,278
Last seen: 2 months, 10 days
Re: Rye jars contamination a few days after shaking? [Re: Corbin19]
    #23798593 - 11/03/16 06:20 PM (7 years, 3 months ago)

Its how I do pretty much every operation in this hobby, don't like inoculation ports. G2G, pouring agar, inoculations, etc, all done by opening the lid.

The key is proper technique. I believe mad season is the author of my favorite writeup on proper technique, surprised I don't see it in his sig.

Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Rye jars contamination a few days after shaking? [Re: Psilosopherr]
    #23798665 - 11/03/16 06:42 PM (7 years, 3 months ago)

I put it back :paranoid: haha I just liked the epic link list and QnA threads. My teks are in the AMU link list as well :smile:


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.

Extras: Filter Print Post Top
OfflineAlCapone2k
Beginner
Male

Registered: 10/06/16
Posts: 842
Loc: Germany Flag
Last seen: 6 years, 5 months
Re: Rye jars contamination a few days after shaking? [Re: Mad Season]
    #23812458 - 11/08/16 11:40 AM (7 years, 3 months ago)

yeah, Mad Seasons tek helped me a lot,  I directly started with a proper SAB and haven't yet faced any severe contamn problem during agar and inculation. This is definetely due to the teks presented here.

So always have these things in mind:

1. Build a SAB (https://www.shroomery.org/forums/showflat.php/Number/20048771#20048771)

2. Wash yourself, take a shower, close the door, all windows, turn off fans, AC, your computer (because of its fans) and so on.

3. Be alone in the room, other persons (like my annoying girlfriend) like to walk around, talk to you so you have to answer and so on. If you have pets, put them in another room.

4. NEVER (!) let your hand go over open media (agar or opened jar). Always get into the media with a flame sterilized tool and ONLY with this tool, not with your hand, not with your wrists, only the sterilized tool is allowed to go over the open media.

5. Use 70% alcohol, but not a litre per day. A bit is enough, clean your gloves at the beginning and during work time after a while re-clean with alcohol but not after every step. If I work for 30 minutes in my SAB, I clean my gloves just twice with alcohol.


Regards :smile:


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: MagicBag.co All-In-One Bags That Don't Suck   Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Injection Grain Bag


Similar ThreadsPosterViewsRepliesLast post
* Post deleted by MOE THE MAD SCIENTIST
( 1 2 all )
vatoloco 10,096 21 04/30/02 03:27 AM
by Zebedee
* I Overcooked my rye jars, am I okay vader 2,242 3 03/24/02 09:57 AM
by nw2shroom
* rehydrating rye jars darshan 1,928 3 10/02/02 06:34 PM
by babyshroom
* Rye jar contams. Joshua 2,555 6 01/22/02 03:49 AM
by MAIA
* Injecting H20 ccs into Rye Jars LucidDayDream 1,750 3 02/15/02 12:46 PM
by kingkc
* Rye jar casing Dephighance 1,101 1 04/03/02 06:57 PM
by Roadkill
* Re: loose lids on rye jars? rebelmoon 2,494 10 05/02/00 08:21 PM
by Anonymous
* quick question about rye jars Chemical_Smile 1,541 6 07/19/17 12:01 AM
by pyro101981

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,246 topic views. 24 members, 265 guests and 80 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.03 seconds spending 0.006 seconds on 12 queries.