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OfflineLevster
Rice Rocketeer


Registered: 08/17/16
Posts: 288
Last seen: 12 hours, 15 minutes
Agar Guidance
    #23797390 - 11/03/16 11:55 AM (7 years, 3 months ago)

Hey guys,

Pretty sure I did something wrong here. I did the agar plates, PC'd them, let them cool over night, then I setup my still air box up, sterilized my needle tip, shook it up nicely to spread the spores and one by one I dripped 1 drop into the center of each agar plate and sealed them back up one by one, sterilizing the needle tip again if the needle surface touched anything.

I couldn't find much on incubation. I put them on the same rack my BRF cake jars sat on. They've been there since The 20th of October, all of the agar plates have nothing on them, some of them have bubbles, and the one in the pic is the only thing with anything on it -- mold.

Where did I go wrong?



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Offlinewtfcrazymofo
foil hater
Other


Registered: 07/26/15
Posts: 1,215
Loc: Colonial alley
Last seen: 4 days, 7 hours
Re: Agar Guidance [Re: Levster]
    #23797456 - 11/03/16 12:13 PM (7 years, 3 months ago)

Maybe a dry agar blend?


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922



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OfflineLevster
Rice Rocketeer


Registered: 08/17/16
Posts: 288
Last seen: 12 hours, 15 minutes
Re: Agar Guidance [Re: wtfcrazymofo]
    #23797929 - 11/03/16 02:47 PM (7 years, 3 months ago)

So not enough water when I cooked it up?
Did I store it correctly? Just upright on the rack with the parafilm tape

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Offlinewtfcrazymofo
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Registered: 07/26/15
Posts: 1,215
Loc: Colonial alley
Last seen: 4 days, 7 hours
Re: Agar Guidance [Re: Levster]
    #23798037 - 11/03/16 03:20 PM (7 years, 3 months ago)

If it is to dry it will take more time for them to germ, so you havn't failed.

You might have cooked to long before loading pp5's = excess water evaporation.

If temps are 60-85 and not moved often= ok storage.


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922



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OfflineKenetic
Nam Sayin
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Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land Flag
Last seen: 5 years, 4 months
Re: Agar Guidance [Re: wtfcrazymofo]
    #23798162 - 11/03/16 03:55 PM (7 years, 3 months ago)

Are you sure that's mold?


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Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet

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Offlinewtfcrazymofo
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Other


Registered: 07/26/15
Posts: 1,215
Loc: Colonial alley
Last seen: 4 days, 7 hours
Re: Agar Guidance [Re: Kenetic]
    #23798360 - 11/03/16 05:01 PM (7 years, 3 months ago)

Maybe not, hard to tell, looks like cobweb, or to much karo or honey in the agar mix.


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922



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OfflineLevster
Rice Rocketeer


Registered: 08/17/16
Posts: 288
Last seen: 12 hours, 15 minutes
Re: Agar Guidance [Re: wtfcrazymofo]
    #23798441 - 11/03/16 05:33 PM (7 years, 3 months ago)

hmm, Well maybe I'll wait another week and then honestly I'll probably just start over....

But yes, I may have cooked the water out of the agar too much, because when I poured it out it wasn't that watery, and it was and is not transparent at all, that could be because I used like 3 drops of food coloring...

I could always crack them open in my SIB and inspect them all, but I don't like handling them too often.


What is the BEST way to store them? with that wad of paper towel on it? without? in a zip lock bag? out in the open?

Thanks friends

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Offlinewtfcrazymofo
foil hater
Other


Registered: 07/26/15
Posts: 1,215
Loc: Colonial alley
Last seen: 4 days, 7 hours
Re: Agar Guidance [Re: Levster]
    #23798479 - 11/03/16 05:42 PM (7 years, 3 months ago)

Do not re-open until you are transfer ready. Go get 8 more rounds.


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922



Extras: Filter Print Post Top
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