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OfflineAK1000
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What's the best way to get a clone from a MS syringe if I'm using agar for reliability?
    #23797192 - 11/03/16 10:49 AM (7 years, 3 months ago)

So, when I started using agar, I started with a MS syringe and kept isolating and after many transfers, I was getting very uniform growth, and then when I tried to grow this on grain, it never fruited, so I'm guessing I got unlucky and isolated non-fruiting genetics.

Now, I've read some comments that say they rather start with a clone (a pin?) and then use that for hte agar instead to ensure that it will fruit anyway.  So how do I go about doing that if i'm starting with a MS syringe and agar?  Do I just put one drop of spore solution from the syringe in the middle of the agar, let that germinate/grow and then do just ONE transfer to ensure clean growth one more time and then tiger-drop that into some grain and hope it fruits?  Then take the first pin I see and put that on some agar and culture that repeatedly?


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-AK

I'm a noob and :heart: this forum. Here's What I've Learned My First FOUR Months.

Best resources: Bods Easy AF List / Ultimate Tek Compendium

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Invisiblepablokabute
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Re: What's the best way to get a clone from a MS syringe if I'm using agar for reliability? [Re: AK1000]
    #23797196 - 11/03/16 10:52 AM (7 years, 3 months ago)

sounds right...

ms fruits to agar to grain spawn to fruiting again


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Offlinethebug76
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Re: What's the best way to get a clone from a MS syringe if I'm using agar for reliability? [Re: pablokabute]
    #23797218 - 11/03/16 11:03 AM (7 years, 3 months ago)

If you only clean it up, and not isolate, it'll pretty much be guarenteed to fruit.


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Offlinespacechildo
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Re: What's the best way to get a clone from a MS syringe if I'm using agar for reliability? [Re: thebug76]
    #23797247 - 11/03/16 11:12 AM (7 years, 3 months ago)

transfer until clean growth, let plate sit until it starts pinning, clone said pin on new agar plate, et voila!

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OfflineNDStepp84
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Re: What's the best way to get a clone from a MS syringe if I'm using agar for reliability? [Re: spacechildo]
    #23797269 - 11/03/16 11:18 AM (7 years, 3 months ago)

:whathesaid: one of my favorite cultures came from an agar pin


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"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread

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InvisiblebodhisattaMDiscordReddit
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Re: What's the best way to get a clone from a MS syringe if I'm using agar for reliability? [Re: NDStepp84]
    #23797326 - 11/03/16 11:35 AM (7 years, 3 months ago)


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OfflineAK1000
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Re: What's the best way to get a clone from a MS syringe if I'm using agar for reliability? [Re: bodhisatta]
    #23797352 - 11/03/16 11:44 AM (7 years, 3 months ago)

Wait, I could have pins form directly on agar? I'm using pasty-plates using the agar recipe he provides, if that matters. How would I initiate pinning on agar?


--------------------
-AK

I'm a noob and :heart: this forum. Here's What I've Learned My First FOUR Months.

Best resources: Bods Easy AF List / Ultimate Tek Compendium

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OfflineGreg
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Re: What's the best way to get a clone from a MS syringe if I'm using agar for reliability? [Re: AK1000]
    #23797363 - 11/03/16 11:45 AM (7 years, 3 months ago)

It only ever happens for me when the plate has been fully colonized for a while and it doesn't happen with all of my cultures.

Here's Pan. cyan. pinning on a petri:


Here is a very contaminated no-pour pinning:

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InvisiblebodhisattaMDiscordReddit
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Re: What's the best way to get a clone from a MS syringe if I'm using agar for reliability? [Re: AK1000]
    #23797380 - 11/03/16 11:52 AM (7 years, 3 months ago)

Quote:

AK1000 said:
How would I initiate pinning on agar?



We don't initiate pinning. The mycelium does. Patience is all you can do

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OfflineAK1000
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Re: What's the best way to get a clone from a MS syringe if I'm using agar for reliability? [Re: bodhisatta]
    #23797392 - 11/03/16 11:56 AM (7 years, 3 months ago)

Quote:

bodhisatta said:
Quote:

AK1000 said:
How would I initiate pinning on agar?



We don't initiate pinning. The mycelium does. Patience is all you can do




Good to know. Would you guys recommend I wait for an agar pin or transfer it to grain and hope for the best? I will be using Madafaka's Bottle Tek to create some clones/mushies cause I got everything necessary to make the coir based bottles now and am kinda excited about that. I just haven't made LI before, though and was thinking of tiger-dropping.


--------------------
-AK

I'm a noob and :heart: this forum. Here's What I've Learned My First FOUR Months.

Best resources: Bods Easy AF List / Ultimate Tek Compendium

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Offlinespacechildo
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Re: What's the best way to get a clone from a MS syringe if I'm using agar for reliability? [Re: spacechildo]
    #23797507 - 11/03/16 12:28 PM (7 years, 3 months ago)

Quote:

spacechildo said:
transfer until clean growth, let plate sit until it starts pinning, clone said pin on new agar plate, et voila!



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OfflineAK1000
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Re: What's the best way to get a clone from a MS syringe if I'm using agar for reliability? [Re: spacechildo]
    #23858747 - 11/22/16 08:52 PM (7 years, 3 months ago)

Quote:

spacechildo said:
Quote:

spacechildo said:
transfer until clean growth, let plate sit until it starts pinning, clone said pin on new agar plate, et voila!







Welp, I went on vacation and came back to find not only an agar pin but an entire mushroom. Now what do I do with this?



It looks beautiful and it dropped its spores on the agar, hehe. :heart:


--------------------
-AK

I'm a noob and :heart: this forum. Here's What I've Learned My First FOUR Months.

Best resources: Bods Easy AF List / Ultimate Tek Compendium

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Offlinekhatinthehat
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Re: What's the best way to get a clone from a MS syringe if I'm using agar for reliability? [Re: AK1000]
    #23858935 - 11/22/16 10:25 PM (7 years, 3 months ago)

If you get a fruit like the above pic on agar you better believe thats a good fruiting strain.

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InvisiblePastywhyteMDiscord
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Re: What's the best way to get a clone from a MS syringe if I'm using agar for reliability? [Re: khatinthehat]
    #23859108 - 11/22/16 11:50 PM (7 years, 3 months ago)

Clone that shit

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OfflineNDStepp84
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Re: What's the best way to get a clone from a MS syringe if I'm using agar for reliability? [Re: Pastywhyte]
    #23859113 - 11/22/16 11:53 PM (7 years, 3 months ago)

:awesomenod:


--------------------

"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread

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OfflineAK1000
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Re: What's the best way to get a clone from a MS syringe if I'm using agar for reliability? [Re: Pastywhyte]
    #23860100 - 11/23/16 09:42 AM (7 years, 3 months ago)

Quote:

Pastywhyte said:
Clone that shit




To clarify, should I simply pick it, then cut open the middle of it with a scalpel and put a piece of the inner tissue on some agar? I've never cloned a pin or mushroom before. I've only used agar for transfers pretty much thus far.


--------------------
-AK

I'm a noob and :heart: this forum. Here's What I've Learned My First FOUR Months.

Best resources: Bods Easy AF List / Ultimate Tek Compendium

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InvisiblebodhisattaMDiscordReddit
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Re: What's the best way to get a clone from a MS syringe if I'm using agar for reliability? [Re: AK1000]
    #23860108 - 11/23/16 09:44 AM (7 years, 3 months ago)

That's basically it. There's videos by RR and munch if you want too

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OfflineAK1000
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Re: What's the best way to get a clone from a MS syringe if I'm using agar for reliability? [Re: khatinthehat]
    #23904937 - 12/07/16 08:00 PM (7 years, 2 months ago)

Quote:

khatinthehat said:
If you get a fruit like the above pic on agar you better believe thats a good fruiting strain.




I found an old pasty plate that was supposed to be contaminated. Turns out it had created more than a few pins!





--------------------
-AK

I'm a noob and :heart: this forum. Here's What I've Learned My First FOUR Months.

Best resources: Bods Easy AF List / Ultimate Tek Compendium

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OfflineBernard
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Re: What's the best way to get a clone from a MS syringe if I'm using agar for reliability? [Re: AK1000]
    #23904968 - 12/07/16 08:10 PM (7 years, 2 months ago)

I'm a noob, but I thought that a better way to go about it was to start with something like a PF TEK BRF cake.  That way, you get a much better idea what you are cloning (size, potency, etc.).

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InvisiblePastywhyteMDiscord
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Re: What's the best way to get a clone from a MS syringe if I'm using agar for reliability? [Re: Bernard]
    #23904988 - 12/07/16 08:15 PM (7 years, 2 months ago)

A clone is always going to need testing etc. But a smaller amount of substrate colonized means fewer cell divisions. Which means more vigor.

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