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OfflinePanMan
Obsession Forever


Registered: 10/05/16
Posts: 62
Last seen: 6 years, 8 months
ID Request - Paneolous Cyanescens or Paneolous Tropicalis
    #23793660 - 11/02/16 04:02 AM (7 years, 3 months ago)

Hi Everyone,

Here is an update to a thread that I posted on October 5th, 2016. Shroomery's Trusted Identifier, Anglerfish responded to it.

See here: https://www.shroomery.org/forums/showflat.php/Number/23709802#23709802

FWIW I decided not to eat any of those.

Followed through with Anglerfish's advice, I went back to that location and scooped up enough cow dung to fill at least 7 Ball pints, spread it out on a tray at about 1 1/2in deep, waited for the mycellium to recover in the SGFC and four different species fruited from it after 72 hours.

I isolated all the mushrooms that fit the bill of a positive paneolous ID in my region that had heavy bruising when stipe was pinched and cap flicked gently, made spore prints (they were black) and refined my cultivation techniques for the next 2 weeks. Still didn't eat any.
 

With some of the black prints I got from the paneolous sp. shown in the previous thread and mushrooms from the cow dung I collected, on October 12th I made some spore rich syringes (90cc total) and inoculated 12 Ball pints and 4 - 12x5x3 trays in a substrate that is 1 1/2in deep (I will case them in a few days). Thankfully there was no contamination. Once the grow has reached its limits for flushes, I anticipate a small harvest of about 7-20 dry grams, anything more is just a bonus.

All cakes started to pin in the jar and they were birthed on October 29th.

Here are the early results.

Something has made me wonder which active paneolous sp. I have been cultivating. See the campanulate cap here







Are those Paneoulous Tropicalis? My intuition tells me Paneolous Cyanescens, however, among the bunch that are still fruiting there are atleast 5 mushrooms that have a campanulate cap and are short and thin at maturity while depositing heavily laced black spores, the others are displaying a convex cap, black spore deposits and are taller (2-5 inches) and thicker. This may be a result of SGFC conditions and FAE but they are in an environment with optimal RH and a PC fan running twice per hour for 5-10 minutes throughout the span of 12 hours.

Here are some more pictures. Shroomery TI's and members, which are they? Pan Cyan or Pan Trop?


















PS: Still haven't eaten any! :laugh:

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Offlinecube talk
Stranger

Registered: 10/11/07
Posts: 1,223
Last seen: 2 months, 13 days
Re: ID Request - Paneolous Cyanescens or Paneolous Tropicalis [Re: PanMan]
    #23793804 - 11/02/16 06:50 AM (7 years, 3 months ago)

off topic but, how many grams did you get out of that method?

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OfflineAnglerfishM
hearing things
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Registered: 09/08/10
Posts: 18,700
Loc: Norvegr Flag
Last seen: 6 hours, 53 minutes
Trusted Identifier
Re: ID Request - Paneolous Cyanescens or Paneolous Tropicalis [Re: PanMan]
    #23793834 - 11/02/16 07:12 AM (7 years, 3 months ago)

So nice to see you got fruits from that shit! :thumbup:

I honestly guess you'll need a microscope or DNA sequencing to tell whether it's P. cyanescens or P. tropicalis, though.

Mind you, I never saw or handled any of these species in real life, only seen them in pictures.


--------------------



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OfflineAlan RockefellerM
Mycologist
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Registered: 03/10/07
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Re: ID Request - Paneolous Cyanescens or Paneolous Tropicalis [Re: Anglerfish] * 1
    #23793983 - 11/02/16 08:57 AM (7 years, 3 months ago)

Quote:

Anglerfish said:

I honestly guess you'll need a microscope or DNA sequencing to tell whether it's P. cyanescens or P. tropicalis, though.







Both microscopy and DNA sequencing should be combined.  I am starting to collect Copelandia sequences.

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OfflinePanMan
Obsession Forever


Registered: 10/05/16
Posts: 62
Last seen: 6 years, 8 months
Re: ID Request - Paneolous Cyanescens or Paneolous Tropicalis [Re: Anglerfish]
    #23793984 - 11/02/16 08:58 AM (7 years, 3 months ago)

Quote:

cube talk said:
off topic but, how many grams did you get out of that method?




It's to early tell as I've just recently introduced the modified pf cakes to the SGFC a few days ago. So far I have 1.5 dried gram. All I can do is some guess work off of what I researched for Pan Cake yield weight, my source however isn't the most reliable (Youtube video) and each grow/grower will have different yield outcomes. From what I've seen on the video the one cake they had flushed on average 10-16 wet grams, dried down to roughly 1 gram. The cake had 5 flushes, so that grower has enough for a super pan heroic dose. I'll post the final dried weight when the mycellium refuses to fruit anymore. I estimate that to be about 2 weeks max.

As for the method I filled up 12 Ball pint jar 3/4 full. I know it's off topic for this thread/forum, but this may be of use for aspiring or tenured growers to get their feet wet with growing Pans. It really isn't that difficult, all those negative thoughts are just limitations that are self imposed because it is something new tackle and goes against auto-pilot routines. Just educate yourself about  growing them. All the resources you could ever ask for are right here on the Shroomery. This is my first ever grow, and I'm glad it was with a Pan Sp. Here is the tek.


~ 10 cups dried field cow dung.

I scooped that up fresh and dried at home, sun and hair blow dryer sped up the drying process.

~ 6 cups really coarse vermiculite.

Cow dung over time will become compressed and not allow wiggle room for growth to 100% colonize. This is to allow for oxygen, unrestricted gas exchange, and mycellium to move freely.

~ 3.5 cups of water.

Field capacity water content was spot on with the RR drip test.

~ 0 BRF. 

~ Pasteurized in a steamer for 1 hr at 150F. Sat overnight


~ No vermiculite layer.

I took the gamble of allowing contamination to happen so there is more room in the jar for the mycellium to have oxygen. I think that was my short coming as all of the jars pinned early indicating the mycellium thinks its 100% colonized but visibly none of the jars showed that the substrate was entirely encompassed like what cube myc typically does. It was just thin whispy mycellium all over with some parts a solid white. Also, the light from the room during incubation could also be the culprit.

~ Added modified filter disk to all jar lids made from a grocery store ecobag.

~ Inoculated 1cc per hole (4 holes on Ball pint lid)

Exactly 3 days for the spores to germinate and see signs of mycellium growth.

2 weeks 5 days for birthing.

~ No dunk and roll.

The cakes were very fragile and prone to crumbling. But I did sprinkle a verm layer on top of each cake and mildly misted directly (1 spray each) then immediately fanned to ensure none of the pins aborted because of the water.

After birthing 24-72 hours for 1.5 dried gram.

I have 4 trays colonizing in a 70/30 cow dung and coarse vermiculite mix right now and have checked them every 4 days to see if there is any signs of contamination. But proper pasteurization and semi-sterile environment (no glove box or flow hood) prevailed this time. I had a face mask and medical gloves on, lysol sprayed vigorously in the air, laid a plastic sheet over a kitchen counter, sprayed 10% bleach water on it, and lit 10 candles... Not for prayer, ritual or chicken sacrifice but to keep most air-born pathogens away while inoculating the jars.

From what I've seen, this Paneolous Sp. gobbles up that cow dung in the trays unlike modified pf cakes, and is evidently the best method for super fast colonization. I have let them colonize a bit longer than I planned considering my Pan PF Cake debacle and early pinning. I am going to case them tomorrow with peat moss and coarse verm at a depth of 1/4 inch.

Quote:

Anglerfish said:
So nice to see you got fruits from that shit! :thumbup:

I honestly guess you'll need a microscope or DNA sequencing to tell whether it's P. cyanescens or P. tropicalis, though.

Mind you, I never saw or handled any of these species in real life, only seen them in pictures.




Yes! That suggestion you made of getting that cow dung has done a lot for me. It allowed me to challenge myself and educate this mind for cultivation. Thank you for the inspiration. This endeavor has been very fulfilling in many aspects.

That'll be my next step, get my hands on a microscope! Whether it's Pan Cyan or Pan Trop, they have exactly the same dosage per psychedelic level.

It's alright to eat the mushrooms pictured in the OP? If so, stick to the less than .5 gram for first ever psychedelic dosage? Also here is more that I'd like to know if I can eat and if they are active. These pics are taken from 2 of the 12 cakes that show the same mushrooms. :smile:












Edited by PanMan (11/02/16 02:45 PM)

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InvisibleByrain

Registered: 01/07/10
Posts: 9,664
Trusted Identifier
Re: ID Request - Paneolous Cyanescens or Paneolous Tropicalis [Re: Alan Rockefeller]
    #23793988 - 11/02/16 08:58 AM (7 years, 3 months ago)

Awesome, it would be great if we could eventually have solid distribution and diversity data on this genus.

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