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OfflinePSILOxPSYCHO
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Registered: 10/02/16
Posts: 17
Last seen: 7 years, 1 month
What to do?
    #23793612 - 11/02/16 02:31 AM (7 years, 2 months ago)

made up some popcorn jars, inoculated some agar with it and found out that there is a bacteria growing along with the mycelium. what shall i do with this jar. should i toss it or would it be safe to spawn a mini tub. any help is appreciated.

Agar is going okay. having a very hard time isolating away from the bacteria, which is a milky yellow/white color. im getting closer i think but im not sure lol. needless to say i started spores on agar and i am looking to do my first transfers on that today sometime. ive got probably 6 or 7 petris on the go right now. i know they say to have patients but im running low on it :thumbdown:


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Invisiblepablokabute
Hari ng Amag
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Registered: 11/22/11
Posts: 5,163
Loc: rural ghetto
Re: What to do? [Re: PSILOxPSYCHO]
    #23793640 - 11/02/16 03:26 AM (7 years, 2 months ago)

if you inoculate a spawn jar with bacterial mycelium then its gonna be 100% contaminated. 

its best you start new spawns with clean agar.


--------------------

Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1

'The second seal: “All CONTAMINATED things and events are unsatisfactory.”'



"I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST."

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OfflinePSILOxPSYCHO
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Registered: 10/02/16
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Re: What to do? [Re: pablokabute]
    #23795701 - 11/02/16 07:55 PM (7 years, 2 months ago)

Quote:

pablokabute said:
if you inoculate a spawn jar with bacterial mycelium then its gonna be 100% contaminated. 

its best you start new spawns with clean agar.




Not to be rude, but did you read my post?
What you told me is known....I don't know why you said that:confused:.

I'm asking what I should do my bacteria contaminated jar of spawn that I have. That's still growing mycelium that looks healthy.

FYI if you read my first post you'll see what I wrote about agar.


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Invisiblepablokabute
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Registered: 11/22/11
Posts: 5,163
Loc: rural ghetto
Re: What to do? [Re: PSILOxPSYCHO]
    #23796025 - 11/02/16 09:48 PM (7 years, 2 months ago)

toss it out with some cow manure and make an outdoor patch.

2-3 weeks and youll have some fruit.


--------------------

Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1

'The second seal: “All CONTAMINATED things and events are unsatisfactory.”'



"I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST."

--Anonymous Guerilla, or is he..


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OfflinePSILOxPSYCHO
Stranger

Registered: 10/02/16
Posts: 17
Last seen: 7 years, 1 month
Re: What to do? [Re: pablokabute]
    #23796213 - 11/02/16 10:53 PM (7 years, 2 months ago)

Quote:

pablokabute said:
toss it out with some cow manure and make an outdoor patch.

2-3 weeks and youll have some fruit.




Is there enough time. I live in Canada. Before long it'll be cold out.

Is it safe to spawn to mini tub in an isolated area? Thanks for the input.


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InvisibleMad Season
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Registered: 09/16/12
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Re: What to do? [Re: PSILOxPSYCHO]
    #23797137 - 11/03/16 10:31 AM (7 years, 2 months ago)

Give it a shot. It's just bacteria. Toss at the first sign of mold. You can also top fruit out of the jar with a casing


--------------------
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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

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No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflinePSILOxPSYCHO
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Registered: 10/02/16
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Re: What to do? [Re: Mad Season]
    #23797556 - 11/03/16 12:48 PM (7 years, 2 months ago)

Quote:

Mad Season said:
Give it a shot. It's just bacteria. Toss at the first sign of mold. You can also top fruit out of the jar with a casing




Care to to guide me to the right tek for that?


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InvisibleMad Season
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Registered: 09/16/12
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Loc: Canada Flag
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Re: What to do? [Re: PSILOxPSYCHO]
    #23797584 - 11/03/16 12:58 PM (7 years, 2 months ago)

Quote:

bodhisatta said:
Quote:

albino said:
Quote:

mushboy said:
which ziplock bag tek are you using?



the one where you use ziploc bags instead of tubs.





no tek just do it



Ziploc bags over jars, or if you have enough jars, throw em in an empty monotub tub. If you have like 1/4-1/2 of the tub filled, you'll have enough surface area to produce decent humidity for you not to need to mist all the time(which is what you'd have to do if you opted for open air)


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflinePSILOxPSYCHO
Stranger

Registered: 10/02/16
Posts: 17
Last seen: 7 years, 1 month
Re: What to do? [Re: Mad Season]
    #23797722 - 11/03/16 01:38 PM (7 years, 2 months ago)

Quote:

Mad Season said:
Quote:

bodhisatta said:
Quote:

albino said:
Quote:

mushboy said:
which ziplock bag tek are you using?



the one where you use ziploc bags instead of tubs.





no tek just do it



Ziploc bags over jars, or if you have enough jars, throw em in an empty monotub tub. If you have like 1/4-1/2 of the tub filled, you'll have enough surface area to produce decent humidity for you not to need to mist all the time(which is what you'd have to do if you opted for open air)




Thanks Mad. Your the man. If I knew how to give you :mushroom2: i would buddy.


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