|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
NwMycoHead
New mycologist



Registered: 08/27/16
Posts: 82
Last seen: 6 years, 10 months
|
Just wanted to share my sucess!! and ask a few??
#23792713 - 11/01/16 07:40 PM (7 years, 2 months ago) |
|
|
First and foremost thank you to all who have helped me get this far I greatly appreciate all the advice and help. Here's a few pics from my last pf tek grow.
 I just knocked up another 16 half pint brf cakes and another 12 small 4oz jars. With some g.t syringes I made myself. This will be my last grow using the pf tek and I plan on moving to rye berries for my spawn and a c/v/g substrate mix. So today I went and got a dozen qt. Jars and some rtv Silicone for self healing injection ports for the lids. Now here lies my questions should I use a L.c for inoculating my grains over a m.s syringe? And is there any lid tek you would recommend in particular? I've seen many different designs and my thought was two 1/4" holes one with Rtv and the other with 3+ layers of micropore tape for g.e. what do you guys think of that would that work for both grain jars and lc jars? Also what's your preference on rye berries over wbs or the other way around?
Edited by NwMycoHead (11/01/16 09:10 PM)
|
irequirechocolate
Stranger

Registered: 10/15/16
Posts: 132
|
Re: Just wanted to share my sucess!! and ask a few?? [Re: NwMycoHead]
#23793091 - 11/01/16 09:20 PM (7 years, 2 months ago) |
|
|
Nice! I think you and I are on a similar path, so I look forward to seeing how things go for the two of us. My plans are to use rye berries w/ agar, tho I will experiment a bit w/ lc too. Sorry I don't really have answers for you, but it's cool to see another beginner finding success. Fun stuff.
|
NwMycoHead
New mycologist



Registered: 08/27/16
Posts: 82
Last seen: 6 years, 10 months
|
Re: Just wanted to share my sucess!! and ask a few?? [Re: irequirechocolate]
#23793122 - 11/01/16 09:29 PM (7 years, 2 months ago) |
|
|
Thanks man definitely curious to agar as well. My main reason for lc is that I've read it can also be a good start for psilocybe cyanescan growth that I can then use to inoculate a large outdoor bed. So I'll definitely be watching your post as well what verities you working with at the moment?
|
NDStepp84
Stellar nuclear waste


Registered: 04/23/15
Posts: 4,956
Last seen: 1 month, 4 days
|
Re: Just wanted to share my sucess!! and ask a few?? [Re: NwMycoHead] 1
#23793150 - 11/01/16 09:39 PM (7 years, 2 months ago) |
|
|
Never start an LC with spores, you can't tell if it's clean or not and chances are it won't be. A proper LC needs to be inoculated with a clean agar wedge, colonized then tested on agar. Spores are dirty, you can get away with it on cakes but grains are less forgiving Personally I wouldn't even use LC unless I had a few dozen jars to inoculate. Start with agar, if you can make jello you can make agar. Check my signature for agar links.
Best filters for grain jars is SFD's or synthetic filter disks. You don't need ships because you can just crack the lid in the SAB. Don't use micropore tape for grain jars, it can cause problems. If you don't use SFDs look into polyfill or Tyvek but SFD's are great. Nice cakes btw
--------------------
"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do. -Robert A. Heinlein
Links and teks ND's grow log and discussion Plant thread
|
morty422
Wuba-luba-dub-dub!


Registered: 07/06/16
Posts: 988
|
Re: Just wanted to share my sucess!! and ask a few?? [Re: NwMycoHead]
#23793151 - 11/01/16 09:39 PM (7 years, 2 months ago) |
|
|
My advice to you is to take a clone from one of your fruits to agar. Make a few transfers to make sure it's clean-and then inoculate a 'master' jar with your grain of choice. I have moved away from WBS and have started using oats. I'm extremely happy with the yields I get from oats and find them easier to use than WBS. I was unable to find Rye where I live so I cannot comment on them.
After inoculating the master, and after it is fully colonized-G2G the master to 12 quart jars and spawn two monotubs...
Clean grain spawn and healthy tubs!
I advise against MS syringe to grain... waste of time in my opinion and a risk-choice in regards to bacteria that the ms syringe more than likely has in it.
You can also take a small piece that you would have filled the master jar with and start another plate to do it again.
Over time, it is best to create a slant that you can keep for a long period of time that won't grow "old".
This method is completely my opinion. Get into agar -and get bigger yields!
|
irequirechocolate
Stranger

Registered: 10/15/16
Posts: 132
|
Re: Just wanted to share my sucess!! and ask a few?? [Re: NwMycoHead]
#23793213 - 11/01/16 10:01 PM (7 years, 2 months ago) |
|
|
Quote:
NwMycoHead said: Thanks man definitely curious to agar as well. My main reason for lc is that I've read it can also be a good start for psilocybe cyanescan growth that I can then use to inoculate a large outdoor bed. So I'll definitely be watching your post as well what verities you working with at the moment?
I have some random stuff. My first batch of 4 cakes was GT, but I have a few other cakes colonizing that are B+ & Huatla, plus 1 burma. I used some GT spores from a syringe on some plates that I *think are healthy, (I will post pics soon to gather opinions). My plan is to shot gun all the cakes I have in about 3 weeks, maybe make a lc from a plate and makes some cakes out of that, (since I still have BRF cake supplies), and then make a monotub w/ the rest of my GT plates if they seem healthy. Because this is new to me, I don't know that I'm ready for outdoor stuff, but let us know how that goes. What region are you in? I'm in a cold area and use space heaters, so it will be interesting to see how things go as the temp starts to dip. Colder temps are supposed to prolong things, so hopefully it doesn't instill bad habits as I try to try to troubleshoot my way through being patient... I'm going to turn my 'reply notification' back on so hopefully we can stay in touch  Good luck!
|
NwMycoHead
New mycologist



Registered: 08/27/16
Posts: 82
Last seen: 6 years, 10 months
|
Re: Just wanted to share my sucess!! and ask a few?? [Re: irequirechocolate]
#23793452 - 11/01/16 11:46 PM (7 years, 2 months ago) |
|
|
Quote:
NDStepp84 said: Never start an LC with spores, you can't tell if it's clean or not and chances are it won't be. A proper LC needs to be inoculated with a clean agar wedge, colonized then tested on agar. Spores are dirty, you can get away with it on cakes but grains are less forgiving Personally I wouldn't even use LC unless I had a few dozen jars to inoculate. Start with agar, if you can make jello you can make agar. Check my signature for agar links.
Best filters for grain jars is SFD's or synthetic filter disks. You don't need ships because you can just crack the lid in the SAB. Don't use micropore tape for grain jars, it can cause problems. If you don't use SFDs look into polyfill or Tyvek but SFD's are great. Nice cakes btw
Thank you, that's the answer I needed. Will definitely stay clear of lc for my cubensis (still going to try lc for some psilocybe cyanescan culture) and go the agar route with that said.
Quote:
morty422 said: My advice to you is to take a clone from one of your fruits to agar. Make a few transfers to make sure it's clean-and then inoculate a 'master' jar with your grain of choice. I have moved away from WBS and have started using oats. I'm extremely happy with the yields I get from oats and find them easier to use than WBS. I was unable to find Rye where I live so I cannot comment on them.
After inoculating the master, and after it is fully colonized-G2G the master to 12 quart jars and spawn two monotubs...
Clean grain spawn and healthy tubs!
I advise against MS syringe to grain... waste of time in my opinion and a risk-choice in regards to bacteria that the ms syringe more than likely has in it.
You can also take a small piece that you would have filled the master jar with and start another plate to do it again.
Over time, it is best to create a slant that you can keep for a long period of time that won't grow "old".
This method is completely my opinion. Get into agar -and get bigger yields!

I've seen alot of debate on wbs/oat/rye/ and even popcorn tek I'm curious roughly what do the oats yield per say 66q tote?
Quote:
irequirechocolate said:
Quote:
NwMycoHead said: Thanks man definitely curious to agar as well. My main reason for lc is that I've read it can also be a good start for psilocybe cyanescan growth that I can then use to inoculate a large outdoor bed. So I'll definitely be watching your post as well what verities you working with at the moment?
I have some random stuff. My first batch of 4 cakes was GT, but I have a few other cakes colonizing that are B+ & Huatla, plus 1 burma. I used some GT spores from a syringe on some plates that I *think are healthy, (I will post pics soon to gather opinions). My plan is to shot gun all the cakes I have in about 3 weeks, maybe make a lc from a plate and makes some cakes out of that, (since I still have BRF cake supplies), and then make a monotub w/ the rest of my GT plates if they seem healthy. Because this is new to me, I don't know that I'm ready for outdoor stuff, but let us know how that goes. What region are you in? I'm in a cold area and use space heaters, so it will be interesting to see how things go as the temp starts to dip. Colder temps are supposed to prolong things, so hopefully it doesn't instill bad habits as I try to try to troubleshoot my way through being patient... I'm going to turn my 'reply notification' back on so hopefully we can stay in touch  Good luck!
Definitely look forward to seeing your plates man. Nice mix I also have g.t and B+ but have been really wanting to order some Cambodians and this Texas orange cap I keep reading about. And I live in the beautiful pnw region.
|
mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
|
Re: Just wanted to share my sucess!! and ask a few?? [Re: morty422]
#23793711 - 11/02/16 05:04 AM (7 years, 2 months ago) |
|
|
Quote:
morty422 said: My advice to you is to take a clone from one of your fruits to agar. Make a few transfers to make sure it's clean-and then inoculate a 'master' jar with your grain of choice. I have moved away from WBS and have started using oats. I'm extremely happy with the yields I get from oats and find them easier to use than WBS. I was unable to find Rye where I live so I cannot comment on them.
After inoculating the master, and after it is fully colonized-G2G the master to 12 quart jars and spawn two monotubs...
Clean grain spawn and healthy tubs!
I advise against MS syringe to grain... waste of time in my opinion and a risk-choice in regards to bacteria that the ms syringe more than likely has in it.
You can also take a small piece that you would have filled the master jar with and start another plate to do it again.
Over time, it is best to create a slant that you can keep for a long period of time that won't grow "old".
This method is completely my opinion. Get into agar -and get bigger yields!

im using oats need to get my lazy arse into gear instead of looking at a massive sack of oats in my grow room  Good stuff op
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
|
Th3Issu3
Stranger Danger


Registered: 12/30/14
Posts: 671
Loc: fucktown
Last seen: 1 month, 23 days
|
Re: Just wanted to share my sucess!! and ask a few?? [Re: mrmazdarx9]
#23793716 - 11/02/16 05:23 AM (7 years, 2 months ago) |
|
|
Make your kids (lids) like this 
As everyone said don't go lc to grain, it's not the safest way.
Agar is easy enough to get into and you'll be able to make a safer decision on what you use to nocc your grain up with.
Use a ms syringe too, not a terrible choice, good luck !
Edited by Th3Issu3 (11/02/16 05:42 AM)
|
mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
|
Re: Just wanted to share my sucess!! and ask a few?? [Re: Th3Issu3]
#23793719 - 11/02/16 05:26 AM (7 years, 2 months ago) |
|
|
Quote:
Th3Issu3 said: Make your kids like this
I make my kids different to that mine are usually little people shaped
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
|
Th3Issu3
Stranger Danger


Registered: 12/30/14
Posts: 671
Loc: fucktown
Last seen: 1 month, 23 days
|
Re: Just wanted to share my sucess!! and ask a few?? [Re: mrmazdarx9]
#23793730 - 11/02/16 05:41 AM (7 years, 2 months ago) |
|
|
It's 7am, gotta shoot me a little slack! Hahahahhaha
|
mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
|
Re: Just wanted to share my sucess!! and ask a few?? [Re: Th3Issu3]
#23793740 - 11/02/16 05:52 AM (7 years, 2 months ago) |
|
|
I hear that
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
|
morty422
Wuba-luba-dub-dub!


Registered: 07/06/16
Posts: 988
|
Re: Just wanted to share my sucess!! and ask a few?? [Re: NwMycoHead]
#23793748 - 11/02/16 05:59 AM (7 years, 2 months ago) |
|
|
If I'm not pulling 9-10 oz dry off of the first flush on a 66qt tub - something went wrong.
You can easily grab a dry onion off of every quart of spawn you use (on the first flush) in your tub as long as it is clean grain. 2oz dry on every quart if you can really get field capacity, conditions and grain moisture content on lock. Hell, I bet Pasty is pulling 13 oz dry off of 5 quarts of spawn by now...
All together off of a 66qt tub, through its entire life-I usually see at least 15oz dry.
I have so many tubs going though-I usually don't have room to keep them around after their 2nd flush.
I have 3 stacks of 5 tubs and 1 stack of 4 - total of 19 tubs going...
The wife says I'm not allowed to buy any more tubs. She says to "Work with what I've got..." 
|
mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
|
Re: Just wanted to share my sucess!! and ask a few?? [Re: morty422]
#23793753 - 11/02/16 06:03 AM (7 years, 2 months ago) |
|
|
Quote:
morty422 said: If I'm not pulling 9-10 oz dry off of the first flush on a 66qt tub - something went wrong.
You can easily grab a dry onion off of every quart of spawn you use (on the first flush) in your tub as long as it is clean grain. 2oz dry on every quart if you can really get field capacity, conditions and grain moisture content on lock. Hell, I bet Pasty is pulling 13 oz dry off of 5 quarts of spawn by now...
All together off of a 66qt tub, through its entire life-I usually see at least 15oz dry.
I have so many tubs going though-I usually don't have room to keep them around after their 2nd flush.
I have 3 stacks of 5 tubs and 1 stack of 4 - total of 19 tubs going...
The wife says I'm not allowed to buy any more tubs. She says to "Work with what I've got..." 

time for a divorce
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
|
morty422
Wuba-luba-dub-dub!


Registered: 07/06/16
Posts: 988
|
Re: Just wanted to share my sucess!! and ask a few?? [Re: mrmazdarx9]
#23793818 - 11/02/16 06:59 AM (7 years, 2 months ago) |
|
|
Quote:
mrmazdarx9 said:
Quote:
morty422 said: If I'm not pulling 9-10 oz dry off of the first flush on a 66qt tub - something went wrong.
You can easily grab a dry onion off of every quart of spawn you use (on the first flush) in your tub as long as it is clean grain. 2oz dry on every quart if you can really get field capacity, conditions and grain moisture content on lock. Hell, I bet Pasty is pulling 13 oz dry off of 5 quarts of spawn by now...
All together off of a 66qt tub, through its entire life-I usually see at least 15oz dry.
I have so many tubs going though-I usually don't have room to keep them around after their 2nd flush.
I have 3 stacks of 5 tubs and 1 stack of 4 - total of 19 tubs going...
The wife says I'm not allowed to buy any more tubs. She says to "Work with what I've got..." 

time for a divorce 
I mean... I need at least 1 more. Make it an even 20. Haha
|
mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
|
Re: Just wanted to share my sucess!! and ask a few?? [Re: morty422] 1
#23793842 - 11/02/16 07:17 AM (7 years, 2 months ago) |
|
|
imagine when it comes time to write down the reason for divorce, "wouldnt let me growz shroomz"
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
|
morty422
Wuba-luba-dub-dub!


Registered: 07/06/16
Posts: 988
|
Re: Just wanted to share my sucess!! and ask a few?? [Re: mrmazdarx9] 1
#23793871 - 11/02/16 07:50 AM (7 years, 2 months ago) |
|
|
Quote:
mrmazdarx9 said:
imagine when it comes time to write down the reason for divorce, "wouldnt let me growz shroomz" 
|
spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
|
Re: Just wanted to share my sucess!! and ask a few?? [Re: morty422]
#23794430 - 11/02/16 12:09 PM (7 years, 2 months ago) |
|
|
9-10 off first flush is that with a isolate? Or just a clone?
|
NwMycoHead
New mycologist



Registered: 08/27/16
Posts: 82
Last seen: 6 years, 10 months
|
Re: Just wanted to share my sucess!! and ask a few?? [Re: morty422]
#23794661 - 11/02/16 01:52 PM (7 years, 2 months ago) |
|
|
Quote:
Th3Issu3 said: Make your kids (lids) like this  As everyone said don't go lc to grain, it's not the safest way.
Agar is easy enough to get into and you'll be able to make a safer decision on what you use to nocc your grain up with.
Use a ms syringe too, not a terrible choice, good luck !
Thank you, I like that lid design. Are those 1/4" holes? And thank you yeah I've been doing alot of research and realize that is def the wrong way. I think I'm gonna start with the grocery store agar tek using 4oz wide mouth jars. And use that to isolate a good g.t. and B+ culture. Then to grains. What do you use as a filter on those lids?
Quote:
morty422 said: If I'm not pulling 9-10 oz dry off of the first flush on a 66qt tub - something went wrong.
You can easily grab a dry onion off of every quart of spawn you use (on the first flush) in your tub as long as it is clean grain. 2oz dry on every quart if you can really get field capacity, conditions and grain moisture content on lock. Hell, I bet Pasty is pulling 13 oz dry off of 5 quarts of spawn by now...
All together off of a 66qt tub, through its entire life-I usually see at least 15oz dry.
I have so many tubs going though-I usually don't have room to keep them around after their 2nd flush.
I have 3 stacks of 5 tubs and 1 stack of 4 - total of 19 tubs going...
The wife says I'm not allowed to buy any more tubs. She says to "Work with what I've got..." 

Perfect, very informative answer I appreciate it couldn't be more spot on. That's way more my pace. Haha. These cakes have been great but just not enough if ya know what I mean. Lol. I'd like to get a few stacks going as well. Wife actually is super intrigued by my new hobby and is starting her own morel garden when the time comes. And helping with my cyan patch so well see how long that lasts. Haha.
Morty and Mr.Mazda thanks for the good laugh too. Haha. That was good.
|
glowdiver
noob



Registered: 06/29/15
Posts: 42
Last seen: 3 months, 7 days
|
Re: Just wanted to share my sucess!! and ask a few?? [Re: NwMycoHead]
#23794766 - 11/02/16 02:33 PM (7 years, 2 months ago) |
|
|
You might check out Pastywhyte's Agar Tek. It's a great way to get started on agar. https://www.shroomery.org/forums/showflat.php/Number/19208415
As for grains I've been using corn, but after I use up these two bags I've already got, I'm going to try and find some rye. I can get a 40lb. bag of "deer corn" for $4.50 right now, but my prep time and contam rates are frustrating. I'm thinking about just feeding it to the squirrels.
Looks like your off to a good start. Congrats on your grow.
Edited by glowdiver (11/02/16 02:39 PM)
|
Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
|
Re: Just wanted to share my sucess!! and ask a few?? [Re: glowdiver]
#23794804 - 11/02/16 02:50 PM (7 years, 2 months ago) |
|
|
If you have a sab like you should anyway you can just put a synthetic filter disc on top like this:

and be done with it. Just pop the lid off and inoculate with whatever you plan to inoculate with. Make sure to wipe down the jar with an alcohol-soaked paper towel.
If you want to get into agar pasty's method is very successful but I've never used it and just jumped straight to pour agar and disposable petris. It's actually way easier than I thought and I'm sure pasty plates are even easier. Just pick one after looking at all the pro's and con's, which I won't discuss here.
Good luck and get busy!
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
|
Th3Issu3
Stranger Danger


Registered: 12/30/14
Posts: 671
Loc: fucktown
Last seen: 1 month, 23 days
|
Re: Just wanted to share my sucess!! and ask a few?? [Re: Kenetic]
#23796814 - 11/03/16 06:59 AM (7 years, 2 months ago) |
|
|
You can get the self healing injection ports in 3 different sizes...
|
Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
|
Re: Just wanted to share my sucess!! and ask a few?? [Re: Th3Issu3] 1
#23797397 - 11/03/16 11:58 AM (7 years, 2 months ago) |
|
|
Why get SHITs? I mean SHIPS.. Shits are like how they sound. Shit and literally never need to be used. I guess I could see them being useful when you have lots to inoc with a liquid, but I've literally never had the need for them, and opening lids all the time really gets you good at sterile techniques
|
Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
|
Re: Just wanted to share my sucess!! and ask a few?? [Re: Mad Season]
#23798127 - 11/03/16 03:43 PM (7 years, 2 months ago) |
|
|
Damn right.
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
|
NwMycoHead
New mycologist



Registered: 08/27/16
Posts: 82
Last seen: 6 years, 10 months
|
Re: Just wanted to share my sucess!! and ask a few?? [Re: Mad Season]
#23799419 - 11/03/16 11:27 PM (7 years, 2 months ago) |
|
|
Quote:
kenetic said: If you have a sab like you should anyway you can just put a synthetic filter disc on top like this:

and be done with it. Just pop the lid off and inoculate with whatever you plan to inoculate with. Make sure to wipe down the jar with an alcohol-soaked paper towel.
If you want to get into agar pasty's method is very successful but I've never used it and just jumped straight to pour agar and disposable petris. It's actually way easier than I thought and I'm sure pasty plates are even easier. Just pick one after looking at all the pro's and con's, which I won't discuss here.
Good luck and get busy!
Thank you for the reply. I have a sab. And definitely am gonna go sfds. Gonna order first thing next week after getting together everything else been reading up on the pasty agar tek as well think it's the best route in my current situation, just for now then I'll move to mea pour agar and Petris
Quote:
Mad Season said: Why get SHITs? I mean SHIPS.. Shits are like how they sound. Shit and literally never need to be used. I guess I could see them being useful when you have lots to inoc with a liquid, but I've literally never had the need for them, and opening lids all the time really gets you good at sterile techniques
Yeah I realized the only real use is to get started using ms otherwise there pointless with g2g or agar to grain so what I did was make 10qt jars with two 1/4" holes used red rtv on one side for a "shit" and the other will be filled/covered with polyfill/micropore tape. I'll be ordering sfds next week after gathering all the other essentials as well and ordering what I can't find locally. I'll use the sfds to make another dozen or two jars to use for grains and retire the 10 I made to use for lc for my other projects. That way I can use them for the first run to get a good clean jar from MS for both my g.t and B+ then just g2g to expand. Does that sound like a good route or? And thanks again for the reply.
|
|