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OfflineKenetic
Nam Sayin
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Registered: 08/24/14
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Re: Just wanted to share my sucess!! and ask a few?? [Re: glowdiver]
    #23794804 - 11/02/16 02:50 PM (7 years, 2 months ago)

If you have a sab like you should anyway you can just put a synthetic filter disc on top like this:



and be done with it.  Just pop the lid off and inoculate with whatever you plan to inoculate with. Make sure to wipe down the jar with an alcohol-soaked paper towel. 

If you want to get into agar pasty's method is very successful but I've never used it and just jumped straight to pour agar and disposable petris.  It's actually way easier than I thought and I'm sure pasty plates are even easier.  Just pick one after looking at all the pro's and con's, which I won't discuss here.

Good luck and get busy!


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Todo Cambia
   

               
                                                :cookiemonster::elmo:



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OfflineTh3Issu3
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Registered: 12/30/14
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Re: Just wanted to share my sucess!! and ask a few?? [Re: Kenetic]
    #23796814 - 11/03/16 06:59 AM (7 years, 2 months ago)

You can get the self healing injection ports in 3 different sizes...


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InvisibleMad Season
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Re: Just wanted to share my sucess!! and ask a few?? [Re: Th3Issu3] * 1
    #23797397 - 11/03/16 11:58 AM (7 years, 2 months ago)

Why get SHITs? I mean SHIPS.. Shits are like how they sound. Shit and literally never need to be used. I guess I could see them being useful when you have lots to inoc with a liquid, but I've literally never had the need for them, and opening lids all the time really gets you good at sterile techniques


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OfflineKenetic
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Re: Just wanted to share my sucess!! and ask a few?? [Re: Mad Season]
    #23798127 - 11/03/16 03:43 PM (7 years, 2 months ago)

Damn right.


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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OfflineNwMycoHead
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Registered: 08/27/16
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Re: Just wanted to share my sucess!! and ask a few?? [Re: Mad Season]
    #23799419 - 11/03/16 11:27 PM (7 years, 2 months ago)

Quote:

kenetic said:
If you have a sab like you should anyway you can just put a synthetic filter disc on top like this:



and be done with it.  Just pop the lid off and inoculate with whatever you plan to inoculate with. Make sure to wipe down the jar with an alcohol-soaked paper towel. 

If you want to get into agar pasty's method is very successful but I've never used it and just jumped straight to pour agar and disposable petris.  It's actually way easier than I thought and I'm sure pasty plates are even easier.  Just pick one after looking at all the pro's and con's, which I won't discuss here.

Good luck and get busy!




Thank you for the reply. I have a sab. And  definitely am gonna go sfds. Gonna order first thing next week after getting together everything else been reading up on the pasty agar tek as well think it's the best route in my current situation, just for now then I'll move to mea pour agar and Petris
Quote:

Mad Season said:
Why get SHITs? I mean SHIPS.. Shits are like how they sound. Shit and literally never need to be used. I guess I could see them being useful when you have lots to inoc with a liquid, but I've literally never had the need for them, and opening lids all the time really gets you good at sterile techniques



Yeah I realized the only real use is to get started using ms otherwise there pointless with g2g or agar to grain so what I did was make 10qt jars with two 1/4" holes used red rtv on one side for a "shit" and the other will be filled/covered with polyfill/micropore tape. I'll be ordering sfds next week after gathering all the other essentials as well and ordering what I can't find locally. I'll use the sfds to make another dozen or two jars to use for grains and retire the 10 I made to use for lc for my other projects. That way I can use them for the first run to get a good clean jar from MS for both my g.t and B+ then just g2g to expand. Does that sound like a good route or? And thanks again for the reply.


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