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OfflineEdmunter
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Re: Mexicana grow [Re: dankington]
    #23791470 - 11/01/16 02:02 PM (7 years, 3 months ago)

Thanks but ive decided to do a filtered grain soak which has gypsum and coffee in it anyway.

Should be ok?

Might keep some for an experiment on plates too.  Can never have too many plates right?

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Offlineblackout
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Re: Mexicana grow [Re: dankington]
    #23792095 - 11/01/16 05:03 PM (7 years, 3 months ago)

Quote:

dankington said:
LC recipes should be like agar recipes, without the agar.




I have found a lot less sugar works better, as recommended by Workman of sporeworks. I thought he made a typo at first but he confirmed he used 0.1-0.5% sugars in LC, while agar is typically 2-4%.

It still gives plenty of growth, it is a tiny bit saving on materials, but has advantages of being clearer too, I find it less "clumpy" and so easier to draw into syringes. The reasoning given was also that it was not wallowing in its own waste (which I think is the same as what he called hypertonic stress) and closer to being stored in a dilute water solution, which is more suited to long term storage.

Pretty sure he is the only one I saw recommending such low concentrations, I have tried it many times and it works well. Others have indirectly sort of recommended it by using only grain soak water which would also have low levels of sugar & nutrients.

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Offlinetombosley8
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Re: Mexicana grow [Re: blackout]
    #23792107 - 11/01/16 05:06 PM (7 years, 3 months ago)

I've been doing 1g malt extract per 600ml water.

I thought that is what TL's LC tek calls for...

let me recheck that.. yep so why so low there?
Quote:

TranscendingLife said:
- Measure out 400mL of water per pint jar, or 600mL of water per quart jar.

- Measure out .667g of (EL)ME per pint, or 1g per quart.




0.16667% nutrition content

Am I getting that right?

I feel like my leap off is slow maybe too low nutrition but I'm not sure.


0.5% seems way to high though so maybe I'll try more like 0.25%



--------------------

Edited by tombosley8 (11/01/16 07:08 PM)

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Invisibledankington
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Re: Mexicana grow [Re: blackout]
    #23792137 - 11/01/16 05:16 PM (7 years, 3 months ago)

Quote:

blackout said:
Quote:

dankington said:
LC recipes should be like agar recipes, without the agar.




I have found a lot less sugar works better, as recommended by Workman of sporeworks. I thought he made a typo at first but he confirmed he used 0.1-0.5% sugars in LC, while agar is typically 2-4%.

It still gives plenty of growth, it is a tiny bit saving on materials, but has advantages of being clearer too, I find it less "clumpy" and so easier to draw into syringes. The reasoning given was also that it was not wallowing in its own waste (which I think is the same as what he called hypertonic stress) and closer to being stored in a dilute water solution, which is more suited to long term storage.

Pretty sure he is the only one I saw recommending such low concentrations, I have tried it many times and it works well. Others have indirectly sort of recommended it by using only grain soak water which would also have low levels of sugar & nutrients.




I apologize for the miscommunication. I meant in regards to the contents of the recipe, not necessarily the ratios. When OP said he wanted to use coffee, gypsum and maple syrup, I thought it was a pretty bad idea.

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OfflineEdmunter
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Re: Mexicana grow [Re: dankington]
    #23792315 - 11/01/16 06:02 PM (7 years, 3 months ago)

Quote:

dankington said:
Quote:

blackout said:
Quote:

dankington said:
LC recipes should be like agar recipes, without the agar.




I have found a lot less sugar works better, as recommended by Workman of sporeworks. I thought he made a typo at first but he confirmed he used 0.1-0.5% sugars in LC, while agar is typically 2-4%.

It still gives plenty of growth, it is a tiny bit saving on materials, but has advantages of being clearer too, I find it less "clumpy" and so easier to draw into syringes. The reasoning given was also that it was not wallowing in its own waste (which I think is the same as what he called hypertonic stress) and closer to being stored in a dilute water solution, which is more suited to long term storage.

Pretty sure he is the only one I saw recommending such low concentrations, I have tried it many times and it works well. Others have indirectly sort of recommended it by using only grain soak water which would also have low levels of sugar & nutrients.




I apologize for the miscommunication. I meant in regards to the contents of the recipe, not necessarily the ratios. When OP said he wanted to use coffee, gypsum and maple syrup, I thought it was a pretty bad idea.




I think I agree,it was a brain fart.  However I think the filtered grain water that has gypsum and coffee added may just be ok?

Thoughts?

I dont really need to do this but I havent made a LC since the bad old days and wanted to do it right.

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OfflineSpore Ninja
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Re: Mexicana grow [Re: filthyknees]
    #23792524 - 11/01/16 07:00 PM (7 years, 3 months ago)

Quote:

filthyknees said:
If your goal is sclerotia formation try 10% coffee agar




Ohhh...wicked cool.... gotta try that.

Anyone done coffee and brown rice?

I'm doing Pollocks brown rice cake recipe vs. some precooked brown rice now. I added coffee grounds to some of each to see what happens.
They should be sprouting myc tomorrow.


--------------------
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Anyone have pix of his Shroommobile RV??

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OfflineEdmunter
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Re: Mexicana grow [Re: Spore Ninja]
    #23796988 - 11/03/16 09:05 AM (7 years, 3 months ago)

What causes this

Im going to make a third transfer away from a potential contamination on the 2nd.  Going back to 10:10 A v DG  on this one.  I have some AA+ and India Orissa that responded really well to the The 8:10 ratio.  I have some PE myc just showing up from my cotton bud swipe which I may put on that to kick start is life with me.

The original spore plate has a few brown bits popping up in it.  Maybe I should transfer a few of these bits away from it.  Pictures would help which I plan to do later.

I am also going to make a MS LC out of one of the first transfers.  Im only really doing it to practice for when I get closer to isolation of both kicking stones and kicking fruit cultures.

There are a few people who us LC for exotics,  why is that?

Edited by Edmunter (11/03/16 11:43 AM)

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Offlineoakley
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Re: Mexicana grow [Re: Edmunter]
    #23797060 - 11/03/16 09:50 AM (7 years, 3 months ago)

that looks like sectoring to me.

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OfflineEdmunter
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Re: Mexicana grow [Re: oakley]
    #23797357 - 11/03/16 11:45 AM (7 years, 3 months ago)

Quote:

oakley said:
that looks like sectoring to me.




Great I thought I may have piggy backed the first contam I saw on plate 1.

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Offlineblackout
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Re: Mexicana grow [Re: tombosley8]
    #23797993 - 11/03/16 03:10 PM (7 years, 3 months ago)

Quote:

tombosley8 said:
I've been doing 1g malt extract per 600ml water.

I thought that is what TL's LC tek calls for...

let me recheck that.. yep so why so low there?
Quote:

TranscendingLife said:
- Measure out 400mL of water per pint jar, or 600mL of water per quart jar.

- Measure out .667g of (EL)ME per pint, or 1g per quart.




0.16667% nutrition content

Am I getting that right?




Yes that is 0.167%, don't think I have never seen his tek before. Most other teks recommend 2-4%.

This is a clone of one of Aero's stones, which were cloned from commercial stones, which is almost definitely ATL#7



I don't recall seeing any browning or stones on these plates, but Aeros grows were the fastest producing sclerotia I have ever seen. His original thread here https://www.shroomery.org/forums/showflat.php/Number/20833312/fpart/1/vc/1

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OfflineEdmunter
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Re: Mexicana grow [Re: blackout]
    #23798025 - 11/03/16 03:17 PM (7 years, 3 months ago)

So much info my head is spinning.  Its all different, all works and  all have pros and cons. Im going to go at it with different teks and compare.

I think I can go for a stone isolate as a separate project and keep MS grows going, clone grows and some brown sector transfers too.  Im mean whats stopping me

My goal is get a load of prints and 2 amazing isolates I can slant, one fruiter, one stone producer.

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OfflinecronicrFacebook
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Re: Mexicana grow [Re: Edmunter]
    #23798173 - 11/03/16 03:58 PM (7 years, 3 months ago)

Quote:

Edmunter said:
So much info my head is spinning.  Its all different, all works and  all have pros and cons. Im going to go at it with different teks and compare.

I think I can go for a stone isolate as a separate project and keep MS grows going, clone grows and some brown sector transfers too.  Im mean whats stopping me

My goal is get a load of prints and 2 amazing isolates I can slant, one fruiter, one stone producer.



For fruiting I simply went with jalisco...Chicon for stones


--------------------

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I'm tired do me a favor

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Offlineoakley
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Re: Mexicana grow [Re: cronicr]
    #23798547 - 11/03/16 06:05 PM (7 years, 3 months ago)

Funny thing, chicon was a horrible stone producer when it first came out. if you read any of the many posts about chicon nindo by elfstone you'd realize that the fruits of chicon are supposed to be special for the clarity and beauty of the trip they produce. so fruit chicon perhaps.

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OfflineEdmunter
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Re: Mexicana grow [Re: oakley]
    #23799624 - 11/04/16 01:58 AM (7 years, 3 months ago)

I think i only have Chicon, but im sure the genetics for both are in there somewhere.  Its going to fun looking.  Just been to the local stable to get my mix ready.

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OfflineEdmunter
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Re: Mexicana grow [Re: Edmunter]
    #23799694 - 11/04/16 03:07 AM (7 years, 3 months ago)

Ive been looking at the growing in a bag way of doing things.  So you colonise and lay the bag out and press it down so its and inch or so thick the whole way.  Stones the size of apples Rogerer Wabit  said.

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OfflineEdmunter
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Re: Mexicana grow [Re: Edmunter]
    #23800555 - 11/04/16 12:32 PM (7 years, 3 months ago)

So ive just done a third transfer from a suspect plate and dropped some genetics in some fltered grain water to make a LC from a clean plate.

I think this might just be where the wedge has moved or a little myc dropped by the main wedge.  I dont think its contamination.  What do you think?



I have a cleaner looking one that im going to let grow before making a few transfers.

Then I think im good to go on my first grow.

Gunna use some Hpoo rich, dog food, straw, gypsum, rye grass seed kind of a Tubs.  Sterilise them, make a cheeky LI and inoc a few for fruiting.  Cloning after but thats another story atm, lets get to this point first.

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Re: Mexicana grow [Re: c10h12n2o]
    #23800596 - 11/04/16 12:45 PM (7 years, 3 months ago)

Quote:

c10h12n2o said:
Quote:

Edmunter said:
I think you acting like a fucking know it all is a little bit tedious and people dont like it.

I know what a culture is and taking a wedge from a culture and growing it on another plate is exactly that.  Until I see a culture I call them wedges.  God, I thought this was becoming a prick free zone.  Ive seen you be  condescending to others, it doesnt help.  I think everybody else understood my post except you.

Im way past the point of having to explain myself.

I get your point on the loop but I would only use it twice a year. Sterile cotton buds are just as a easy.


I think we need to start again

Greetings Im Edmunter how do you do?




um, maybe calm down a little bit? if you quit looking for something to take personal and get offended by youd probably agree with me, or at least make sense. you clearly havent heard a word anyone has said to you

i find it hilarious how many cry-bullies we have been having in mush cult lately, that ask misguided misworded questions and then talk down to the people who take the time to answer their question and offer constructive criticism, and then act all butthurt and mistreated when someone puts them in their place with a well-constructed argument

if you cant handle criticism or feedback, maybe dont ask a question... or grow thicker skin so you can have an adult conversation without finding everything "condescending."

Also, maybe dont pick an argument with the guy who's debate scholarships paid his way through college if you cant back it up with anything more than calling someone a "know it all" in the same breath as saying "im way past the point of having to explain myself" :rofl:

if you would drop the animosity/arrogance you would probably agree with me, or at least not take it so personally



Quote:

c10h12n2o said:
Quote:

Edmunter said:
I think you acting like a fucking know it all is a little bit tedious and people dont like it.

I know what a culture is and taking a wedge from a culture and growing it on another plate is exactly that.  Until I see a culture I call them wedges.  God, I thought this was becoming a prick free zone.  Ive seen you be  condescending to others, it doesnt help.  I think everybody else understood my post except you.

Im way past the point of having to explain myself.

I get your point on the loop but I would only use it twice a year. Sterile cotton buds are just as a easy.


I think we need to start again

Greetings Im Edmunter how do you do?




um, maybe calm down a little bit? if you quit looking for something to take personal and get offended by youd probably agree with me, or at least make sense. you clearly havent heard a word anyone has said to you

i find it hilarious how many cry-bullies we have been having in mush cult lately, that ask misguided misworded questions and then talk down to the people who take the time to answer their question and offer constructive criticism, and then act all butthurt and mistreated when someone puts them in their place with a well-constructed argument

if you cant handle criticism or feedback, maybe dont ask a question... or grow thicker skin so you can have an adult conversation without finding everything "condescending."

Also, maybe dont pick an argument with the guy who's debate scholarships paid his way through college if you cant back it up with anything more than calling someone a "know it all" in the same breath as saying "im way past the point of having to explain myself" :rofl:

if you would drop the animosity/arrogance you would probably agree with me, or at least not take it so personally




Ok man, how much more childish can you be? Calm down. You're the one that came on this guys thread being a total condescending grammar nazi. Chill out. Just because he's not using the correct "jargon" since you love to put words people say in quotes to make it sound stupid, you jump all down his throat for petty simple stuff. Nobody really cares if you have debate scholarships. I'm a union electrician, who gives a rats ass? Get your panties out of a wad bud

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Invisibledankington
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Re: Mexicana grow [Re: brickwallnomad]
    #23801015 - 11/04/16 03:55 PM (7 years, 3 months ago)

:lolwut:

says the optout :optout:

No need to stir that pot, bud. That's been over.

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OfflineEdmunter
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Re: Mexicana grow [Re: Edmunter]
    #23803042 - 11/05/16 11:12 AM (7 years, 3 months ago)

So this is the original plate and as you can see its has brown areas.  As RR mentioned transferring brown strains to get the stones genetics. 

Anyone know anything about this and do these look worth trying to you?


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Offlineblackout
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Re: Mexicana grow [Re: Edmunter]
    #23803271 - 11/05/16 12:35 PM (7 years, 3 months ago)

Quote:

Edmunter said:
Anyone know anything about this and do these look worth trying to you?






I would certainly give it a try.

Regarding the names, I have a feeling there might be 2 mexicanas available with the same name but were developed from different original specimens. i.e. they were collected in the same region and so have the same name. I am not certain about that at all though, so someone might correct me. I thought I had seen 2 vendors with the same names but different info in the descriptions.

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