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OfflineTeaSippinHippie
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Registered: 11/09/13
Posts: 621
Loc: small sticks town
Last seen: 3 years, 2 months
Not another Agar post.
    #23792049 - 11/01/16 04:47 PM (7 years, 2 months ago)

Now that I'm in my own home I've been gathering things for agar work,i have the following:

- 1, 120qt SAB.
- 1, 500ml Borosilicate Glass bottle with
  Gl45 lid.
- multispore syringe.
- stainless steel scalpel with 20 #11 
  blades.
- PC
- dry MEA mix.
- 6 Borosillicate glass petris.

I've been doing my reading but feel im missing some information, been following the recent agar pages but just don't feel confident.

Before i begin the log i have a few questions.

-What items will i need in the SAB during.

-What do i wrap the petris in while PCing?
  I read i can put them in a small plastic container, doesn't plastic melt in the PC.

-how many dishes/transfers will it take for a pretty close isolated strain from MS?

Thank you all in advanced i wish i could've done this years ago.

I'm also about able to start donating to the shroomery :aweohyou: love this place.


--------------------
I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE.
MudaFuka said:
There's nothing wrong with making mistakes as long as you don't make the same ones twice.


Edited by TeaSippinHippie (11/01/16 07:02 PM)


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Offlinespacechildo
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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23792081 - 11/01/16 04:58 PM (7 years, 2 months ago)



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OfflineTeaSippinHippie
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Registered: 11/09/13
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Re: Not another Agar post. [Re: spacechildo]
    #23792161 - 11/01/16 05:24 PM (7 years, 2 months ago)

I've read up on all that, i got my basic plan down for preparing the agar, I've been reading a tek by bodhisatta and one by stro.

I just need a few questions answered and guidance along the way till i get a successful grow using agar, but any comments are welcome, i am saving up for more dishes, that kind of glass is expensive though haha


--------------------
I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE.
MudaFuka said:
There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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Offlinespacechildo
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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23792284 - 11/01/16 05:54 PM (7 years, 2 months ago)

why buy glass if you're doing pour agar?


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OfflineTeaSippinHippie
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Registered: 11/09/13
Posts: 621
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Last seen: 3 years, 2 months
Re: Not another Agar post. [Re: spacechildo]
    #23792507 - 11/01/16 06:55 PM (7 years, 2 months ago)

I'll be trying both, and i like that i can re use them, and put them in the oven if i need to.


--------------------
I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE.
MudaFuka said:
There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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Invisibledankington
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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23792566 - 11/01/16 07:11 PM (7 years, 2 months ago)

you'll soon grow very tired of working that way. When you start going through 20+ plates a week, you'll understand. If you're being environmentally friendly, do no-pour. Otherwise, do yourself a favor and buy a few hundred dishes.


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Offlinenumnum59
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Re: Not another Agar post. [Re: dankington]
    #23792624 - 11/01/16 07:23 PM (7 years, 2 months ago)

listen to these guys, i started agar maybe 2 weeks ago and im through 50 dishes so far. i used pastywhytes no pour tek for 25 of them and its awesome because they are reusable. im going to use them to isolate then xfer to a petri for storage.


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OfflineSpore Ninja
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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23792666 - 11/01/16 07:30 PM (7 years, 2 months ago)

Quote:

TeaSippinHippie said:
Now that I'm in my own home I've been gathering things for agar work,i have the following:

- 1, 120qt SAB.
- 1, 500ml Borosilicate Glass bottle with
  Gl45 lid.
- multispore syringe.
- stainless steel scalpel with 20 #11 
  blades.
- PC
- dry MEA mix.
- 5 pyrex glass petris.

I've been doing my reading but feel im missing some information, been following the recent agar pages but just don't feel confident.

Before i begin the log i have a few questions.

-What items will i need in the SAB during.

-What do i wrap the petris in while PCing?
  I read i can put them in a small plastic container, doesn't plastic melt in the PC.

-how many dishes/transfers will it take for a pretty close isolated strain from MS?

Thank you all in advanced i wish i could've done this years ago.

I'm also about able to start donating to the shroomery :aweohyou: love this place.




Awww..another agar angels gets his wings. Always cool to see.  :goodluck:

Rule 1- don't overthink it- you just need to start pouring and inoculating plates. The more you do, the better you'll get.
Do some "practice" plates first if you want to, so there's no stress about contams or possibly losing your work to unforeseen events.
Heck, you can try pouring some plates in the open air if you just want to practice. Then just re-sterilize if glass, or toss them if they're plastic. (now you can start to see whey disposable plastic makes more sense)
So practice all the steps- cooking agar AND pouring plates til you're comfortable with it. This will answer your 1st question for you.

2- I'd really, really suggest using disposable, pre-sterilized plates. 
You might need 10 or more plates at one time.
Buy 3,4 or 5 sleeves of 100mm plates from FP or a vendor.

If you want to use glass- either buy a rack for them, or wrap them in foil. As far as I know- it's impossible to PC a petri dish with agar in it.
Look at the plastic container's recycling code- if has a "5" inside the triangle, it will survive the PC. I know a 5 (polypropylene or PP) will survive a PC, I don't think any of the other plastics will.

Here's how RR described the isolation process(I'm paraphrasing):
This assumes a clean culture with no contam issues-

Make a small line/wipe of your spores on agar.
AS SOON as the myc appears- cut small sections the size of a grain of rice and transfer them to separate dishes.

Then, let those grow out until you can see some sectoring.

Choose sectors from each dish, and cut small samples to transfer to new dishes.

Choose healthy, vigorous myc from the last transfers to transfer to grain.

Make mini casings or mini tubs to fruit out the grain so you can see which isolate yields the best.


So that means you need 1-3 dishes for your initial swipe(s), then in a couple days, you'll need 5-10 dishes to accept the 1st tiny samples.
Then you'll probably need 10+ dishes to accept the samples after you see sectoring.
You'll need at least 10-15 dishes.

Have you tried mixing some agar and pouring plates yet?? Let's break those babies in!!


--------------------
I'm interested in Dr. Pollock's work and his (especially older) strains.
Anyone have pix of his Shroommobile RV??

PSA: Protonmail offers free, encrypted email hosted in privacy loving Switzerland.
Red Phone / Signal offer free encrypted phone calls and texting between users.

All of these programs are available free for PC and Android or apple and are available in the respective stores or online by googling the names.
Stay safe out there!

The Electronic Freedom Foundation's review of text and messaging program's security is here>>> https://www.eff.org/node/82654


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OfflineTeaSippinHippie
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Registered: 11/09/13
Posts: 621
Loc: small sticks town
Last seen: 3 years, 2 months
Re: Not another Agar post. [Re: numnum59]
    #23792667 - 11/01/16 07:30 PM (7 years, 2 months ago)

I won't be doing agar a lot, so after i buy a few more sets of 6 ill be ok. Can any of you answer my questions up above?


--------------------
I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE.
MudaFuka said:
There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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OfflineTeaSippinHippie
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Registered: 11/09/13
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Loc: small sticks town
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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23792700 - 11/01/16 07:37 PM (7 years, 2 months ago)

Thank you ninja for answering my questions, if i have too many contaminated dishes at first I'll buy some pre sterilized plastic ones and put the glass up till im better and can go through it with less dishes.


--------------------
I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE.
MudaFuka said:
There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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Offlinenumnum59
Pro-Am Mycologist


Registered: 12/07/11
Posts: 1,189
Last seen: 5 months, 22 days
Re: Not another Agar post. [Re: Spore Ninja]
    #23792705 - 11/01/16 07:38 PM (7 years, 2 months ago)

Before i begin the log i have a few questions.

-What items will i need in the SAB during.

-What do i wrap the petris in while PCing?
  I read i can put them in a small plastic container, doesn't plastic melt in the PC.

-how many dishes/transfers will it take for a pretty close isolated strain from MS?


in the sab i usually just have my clean dishes and my dishes im xfering. if im pouring ill have my petri dishes and the bottle with the agar.

not sure about what you wrap them in while pcing, if theyre glass then just put them on a rack or possibly wrap them in tin foil.

it can vary, i have done 2 xfers on several strains so far and im not close to getting an isolate.


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Invisibledankington
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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23792710 - 11/01/16 07:39 PM (7 years, 2 months ago)

You'll have a lot of contams. Could you imagine if I used glass petris? This stack is all garbage to me, and from about 1 week of work :frown:



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OfflineSpore Ninja
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Registered: 10/25/16
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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23792794 - 11/01/16 07:59 PM (7 years, 2 months ago)

Quote:

TeaSippinHippie said:
I won't be doing agar a lot, so after i buy a few more sets of 6 ill be ok. Can any of you answer my questions up above?




Hope I just did.

You also need:
a couple bottles of 70% alcohol
latex or nitrile gloves
paper towels
Oust, or Ozium
maybe some Clorox wipes
an alcohol lamp or good lighter/torch
an inoculating loop (or sterile swabs. You can DIY a good loop - I heard you can use toaster heating elements since they're made from nichrome)

All of that is available from Walmart. You can get a cheap toaster at a thrift store. I scored a set of 7 small plastic bins at Goodwill today that are perfect for mini casings.

DO NOT USE FLAMMABLE ALCOHOL INSIDE A GLOVEBOX WITH FLAME OR A LIGHTER!
(Ask me how I know)


Edited by Spore Ninja (11/01/16 08:17 PM)


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OfflineTeaSippinHippie
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Registered: 11/09/13
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Re: Not another Agar post. [Re: Spore Ninja]
    #23792889 - 11/01/16 08:24 PM (7 years, 2 months ago)

I have most of that already from knocking up spawn bags. Should i keep any cleaning supplies inside the SAB? Like lysol?

And I'm pretty ocd when it comes to being clean before i work, i take shower get out go into work room, spray generous amount of lysol, put clothing on straight out of sterilized cycle on my washer n dryer, wipe area with 99.9 rubbing alcohol, then all tools and anything ill be touching, then my hands, gloves on, hands again then arms, then a hexagon mask.

And thats normally all without the SAB i normally don't use one for just inoculations, but for it ill add extra steps like cleaning the inside of the SAB and placing it on a soapy samp towel.


--------------------
I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE.
MudaFuka said:
There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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OfflineTeaSippinHippie
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Re: Not another Agar post. [Re: dankington]
    #23792953 - 11/01/16 08:44 PM (7 years, 2 months ago)

20 pre sterilized dishes on the way :thumbup:


--------------------
I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE.
MudaFuka said:
There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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OfflineSpore Ninja
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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23793215 - 11/01/16 10:02 PM (7 years, 2 months ago)

Quote:

TeaSippinHippie said:
I have most of that already from knocking up spawn bags. Should i keep any cleaning supplies inside the SAB? Like lysol?

And I'm pretty ocd when it comes to being clean before i work, i take shower get out go into work room, spray generous amount of lysol, put clothing on straight out of sterilized cycle on my washer n dryer, wipe area with 99.9 rubbing alcohol, then all tools and anything ill be touching, then my hands, gloves on, hands again then arms, then a hexagon mask.

And thats normally all without the SAB i normally don't use one for just inoculations, but for it ill add extra steps like cleaning the inside of the SAB and placing it on a soapy samp towel.




Sometimes I'll keep my supplies in my glovebox if I don't have a dedicated room, or anywhere else to stash them.
Sounds like you have things covered pretty well.
There is a bit of an art to pouring agar- it's super messy if you spill it. You should practice pouring it (and cooking it)once or twice so you can see how to handle it.

You need to:
-lift the petri lid
-keep your fingers out of the way
-pour just the right amount of agar into the dish - too much and it spills or overflows and ruins that dish, too little and it might not work at all
-then give the bottle a slight twist as you finish each pour so it      doesn't drip. One misplaced drip can ruin a plate (or a stack!).
-then get the lid back on the peti
-wrap with saran wrap cut into a 2 inch strip, or wrap with Parafilm.
-then repeat it on the next petri in the stack. You work with the pertis in a stack, not dishes laid out individually.

Not really all that difficult, but it takes a bit of practice to get all that just right.
Oh, and you can't go too slow or the agar will harden up in the flask.:grin:


RR has videos showing pours, and there are several on Youtube that show pouring and SAB procedures.
"pouring agar plates"
"using SAB"


--------------------
I'm interested in Dr. Pollock's work and his (especially older) strains.
Anyone have pix of his Shroommobile RV??

PSA: Protonmail offers free, encrypted email hosted in privacy loving Switzerland.
Red Phone / Signal offer free encrypted phone calls and texting between users.

All of these programs are available free for PC and Android or apple and are available in the respective stores or online by googling the names.
Stay safe out there!

The Electronic Freedom Foundation's review of text and messaging program's security is here>>> https://www.eff.org/node/82654


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OfflineTeaSippinHippie
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Re: Not another Agar post. [Re: Spore Ninja]
    #23793759 - 11/02/16 06:07 AM (7 years, 2 months ago)

The glass bottle i got too put the agar mix was recommended by all the big dogs on the site, gl45 lid prevents that unwanted drip.


--------------------
I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE.
MudaFuka said:
There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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OfflineSpore Ninja
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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23794507 - 11/02/16 12:45 PM (7 years, 2 months ago)

Quote:

TeaSippinHippie said:
The glass bottle i got too put the agar mix was recommended by all the big dogs on the site, gl45 lid prevents that unwanted drip.




Sounds like your on a solid path and will do well...lol.. just maybe some 1st time jitters. Practice makes perfect. Don't treat the dishes like delicate little beauty queens, the want to get dirty and get used...often!

What's your first project going to be?


--------------------
I'm interested in Dr. Pollock's work and his (especially older) strains.
Anyone have pix of his Shroommobile RV??

PSA: Protonmail offers free, encrypted email hosted in privacy loving Switzerland.
Red Phone / Signal offer free encrypted phone calls and texting between users.

All of these programs are available free for PC and Android or apple and are available in the respective stores or online by googling the names.
Stay safe out there!

The Electronic Freedom Foundation's review of text and messaging program's security is here>>> https://www.eff.org/node/82654


Edited by Spore Ninja (11/02/16 12:51 PM)


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OfflineTeaSippinHippie
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Re: Not another Agar post. [Re: Spore Ninja]
    #23794585 - 11/02/16 01:28 PM (7 years, 2 months ago)

I just want to mess around at first with MS on the dishes while my spawn bags finish colonizing, get my technique down and watch mycelium grow, get pointers and tips on here.
i might save any good dishes.

Once my bags are done and i spawn them and get a nice specimen i want to clone it.

Im not super nervous, i just wanted some questions answered before i started the fun.


--------------------
I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE.
MudaFuka said:
There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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Offlinenumnum59
Pro-Am Mycologist


Registered: 12/07/11
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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23794619 - 11/02/16 01:39 PM (7 years, 2 months ago)

a couple tips i missed my first time that i wish i would have known.

bodhisatta -after you boil your agar and let the PC cool enough to pull it out "put them in 47ºC water bath. your agar jars are hotter than 47 so the water bath will warm up. once it cools back down to 47ºC then you're ready to pour." this will eliminate condensation on the lids which isnt necessarily a vector but it is annoying trying to see your growths through condensation.

i use a 750ml burtons gin bottle (~$7) for my agar, clean off the label and its long neck is perfect for pouring(any 750ml liquor bottle will work)

i also use brand new ziplock sandwich bags to seal the petri dishes due to the fact seran wrap is a bitch to cut how people say to do it for agar. it works so far for me.

if i think of any more, ill edit the post.


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OfflineTeaSippinHippie
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Re: Not another Agar post. [Re: numnum59]
    #23795190 - 11/02/16 05:01 PM (7 years, 2 months ago)

I think that bod quote is from a page about agar i have saved from him haha


Edited by TeaSippinHippie (11/02/16 05:05 PM)


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OfflineAlCapone2k
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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23795509 - 11/02/16 06:32 PM (7 years, 2 months ago)

I would not pour and wrap, pour the whole stack and then either put all the petris unwrapped in a ziploc bag oder wrap them all after pouring the whole stack with saran wrap/parafilm.

If you pour and wrap every single dish, your agar could cool down and you could get some problems with the last petris, because your agar got sticky.


And yes, the quote is from the "Pour Agar Tek" from bodhi, which is in my opinion the best one to go for.


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


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OfflineTeaSippinHippie
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Registered: 11/09/13
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Re: Not another Agar post. [Re: AlCapone2k]
    #23795625 - 11/02/16 07:22 PM (7 years, 2 months ago)

Am i wraping the whole dish lid and all? Wraping afterwards sounds better so I'll definitely do it that way.


--------------------
I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE.
MudaFuka said:
There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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OfflineGreg
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Re: Not another Agar post. [Re: AlCapone2k]
    #23795628 - 11/02/16 07:24 PM (7 years, 2 months ago)

No need to wrap after pouring.
Only after inoculating as many others have said.

My unused plates sit in stacks in the SAB until I inoculate them.
My unpoured plates sit in stacks just like the poured ones, sometimes I keep the original sleeve on them too. (This doesn't really apply to glass petris though as they don't come in sleeves presterile like my plastic ones do.)

They sit there sometimes upwards of a week without issue.
I've only ever had 3 contam before using them out of 300+ I've poured.

I have two stacks of poured plates, one is regular ME/PD agar and the other is antibacterial agar (dyed red).
I germinate spores and take clones on the AB agar if I have it handy, otherwise I just use regular MEA/PDA.
Everything else is always done on MEA or PDA.



Follow this link to see how I use Parafilm to wrap dishes after inoculating:
https://www.shroomery.org/forums/showflat.php/Number/23717103
You just wrap the edges but it's OK if a little bit overlaps on to the top or bottom of the plate.


Edited by Greg (11/02/16 07:29 PM)


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OfflineTeaSippinHippie
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Re: Not another Agar post. [Re: Greg]
    #23795653 - 11/02/16 07:35 PM (7 years, 2 months ago)

I bought a 20pk of plastic pre sterilized.
Good information thank you :thumbup:


--------------------
I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE.
MudaFuka said:
There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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OfflineSpore Ninja
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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23795942 - 11/02/16 09:17 PM (7 years, 2 months ago)

Quote:

TeaSippinHippie said:
Am i wraping the whole dish lid and all? Wraping afterwards sounds better so I'll definitely do it that way.




Yes, wrap it only after it's inoculated.
With the the plastic petris, you can safely store them back in the original plastic sleeve after you pour them.

If you wrap the glass ones in foil when you PC them they should be OK that way, especially if you store them in a clean SAB. Put them in a gallon size ziplock for an extra level of security if you feel the need.


--------------------
I'm interested in Dr. Pollock's work and his (especially older) strains.
Anyone have pix of his Shroommobile RV??

PSA: Protonmail offers free, encrypted email hosted in privacy loving Switzerland.
Red Phone / Signal offer free encrypted phone calls and texting between users.

All of these programs are available free for PC and Android or apple and are available in the respective stores or online by googling the names.
Stay safe out there!

The Electronic Freedom Foundation's review of text and messaging program's security is here>>> https://www.eff.org/node/82654


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InvisiblebodhisattaMDiscordReddit
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Re: Not another Agar post. [Re: Spore Ninja]
    #23796030 - 11/02/16 09:50 PM (7 years, 2 months ago)

70% alcohol is better at sanitizing than 90+
I saw 99% alcohol on the other page. :warcry:


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OfflineTeaSippinHippie
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Re: Not another Agar post. [Re: bodhisatta]
    #23796821 - 11/03/16 07:04 AM (7 years, 2 months ago)

So i need to get 70 instead?


--------------------
I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE.
MudaFuka said:
There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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Offlinenumnum59
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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23796964 - 11/03/16 08:47 AM (7 years, 2 months ago)

70% percent of alcohol is ideal to a stronger solution. Pure alcohol coagulates protein in contact. Suppose the pure alcohol is poured over a single celled organism. The alcohol will go through the cell wall of the organism in all direction, coagulating the protein just inside the cell wall. The ring of the coagulated protein would then stop the alcohol from penetrating farther from the cell, and no more coagulation would take place. At this time the cell would become inactive but not dead. Under the favorable conditions the cell would then begin to function. If 70 percent of alcohol is poured to a single celled organism, the diluted alcohol also coagulates the protein, but at a slower rate, so that it penetrates all the way through the cell before coagulation can block it. Then the entire cell is coagulated and the organism dies.


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Re: Not another Agar post. [Re: numnum59]
    #23797006 - 11/03/16 09:16 AM (7 years, 2 months ago)

Gotta love science :bongload:


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There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23797032 - 11/03/16 09:34 AM (7 years, 2 months ago)

You can water it down to 70%


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Re: Not another Agar post. [Re: bodhisatta]
    #23797125 - 11/03/16 10:26 AM (7 years, 2 months ago)

I'll go out and get the 70% and toss the strong stuff in the emergency farm bag.


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There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23797172 - 11/03/16 10:40 AM (7 years, 2 months ago)

90+ is good for your bongs


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Re: Not another Agar post. [Re: bodhisatta]
    #23797219 - 11/03/16 11:03 AM (7 years, 2 months ago)

Haha yeah, we have one the bong too.


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There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23800300 - 11/04/16 10:51 AM (7 years, 2 months ago)

Thinking tonights the night.
I got everything i need.


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There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23800311 - 11/04/16 10:54 AM (7 years, 2 months ago)

:fuckinawesome:


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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23800448 - 11/04/16 11:49 AM (7 years, 2 months ago)

Quote:

TeaSippinHippie said:
Thinking tonights the night.
I got everything i need.




You reminded me I need to do so isolation and agar work...cleaning the kitchen now so I can get set up to pour.
Think I'll do dog food agar with some activated charcoal for color.


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Re: Not another Agar post. [Re: Spore Ninja]
    #23800519 - 11/04/16 12:18 PM (7 years, 2 months ago)

I have ziplock bags to store dishes until i put spores to them, but afterwards can i wrap in gladwrap and put back in ziplock? Making sure first.


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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23800577 - 11/04/16 12:39 PM (7 years, 2 months ago)

Quote:

TeaSippinHippie said:
I have ziplock bags to store dishes until i put spores to them, but afterwards can i wrap in gladwrap and put back in ziplock? Making sure first.




I just store them nocd up or not in ziplocks without wrapping them. Just use a new ziplock each time.

Cutting seran wrap is a bitch and a half


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Re: Not another Agar post. [Re: numnum59]
    #23800617 - 11/04/16 12:57 PM (7 years, 2 months ago)

Ok thank you, also the directions on the MEA say 20gs to 500ml, simmer to fully dissolved then sterilize at 10psi for 30 mins. Can i just set the glass bottle in a pot of water to boil? Or do i need to boil it in a small pot then put it in the bottle to PC?

I might just be over thinking this haha


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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23800665 - 11/04/16 01:20 PM (7 years, 2 months ago)



Pretty much just followed what that guy did


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Re: Not another Agar post. [Re: numnum59]
    #23800751 - 11/04/16 02:02 PM (7 years, 2 months ago)

So i can just add MEA and water in the bottle and microwave it? For how long?


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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23800757 - 11/04/16 02:04 PM (7 years, 2 months ago)

nevermind the microwave, you got a PC.


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Re: Not another Agar post. [Re: spacechildo]
    #23800796 - 11/04/16 02:22 PM (7 years, 2 months ago)

So skip the microwave, add both to bottle and mix and just PC? Should i prep the SAB while the PC is cooling down? Then bring the bottle straight to SAB? And wipe everything down again?


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Re: Not another Agar post. [Re: TeaSippinHippie] * 1
    #23800825 - 11/04/16 02:33 PM (7 years, 2 months ago)

mix the MEA and water, boil it so it dissolves. then put into your bottle PC it then cool it down to pouring plates temperature and put it in your SAB. "preparing" a SAB takes like 30 seconds so don't worry about that


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Re: Not another Agar post. [Re: bodhisatta]
    #23800837 - 11/04/16 02:37 PM (7 years, 2 months ago)

I let the agar jars sit inside the PC. They have a nice pouring temperature about 2 to 3 hours after I turned the heat off. Open the PC after 2 hours and check if you can touch the agar container without burning yourself. The cooler the agar mix, the less condensation will be present after pouring.


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Re: Not another Agar post. [Re: enlightenment]
    #23800857 - 11/04/16 02:46 PM (7 years, 2 months ago)

Quote:

Bodhisatta said:
put them in 47ºC water bath. your agar jars are hotter than 47 so the water bath will warm up. once it cools back down to 47ºC then you're ready to pour.


ready to use

if you pour your dishes correctly at the low temperature you'll end up with something like this

near the top of the stack you might get a few dishes with some condensation, I actually like to pour stacks tall, so I do them 10 high, if you do a bunch of short stacks you get a bunch of dishes with a little bit of condensation if you do a stack of 10 you get 7-8 perfect dishes and 2 you may have to fix.

how do you fix them?

use a cup with warm water and a flat bottom, put it on top of the dish for a few seconds-minutes (wont hurt if the water is warm-hot)
and boom your dishes are crystal clear again.



Why 47?

Quote:

Agar exhibits hysteresis, melting at 85 °C (358 K, 185 °F) and solidifying from 32–40 °C (305–313 K, 90–104 °F). This property lends a suitable balance between easy melting and good gel stability at relatively high temperatures.



I find it starts to gel back up at about 107F or 42C most of the time at the gel strength used for plates. at 47C you can pour a whole sleeve of plates before it gels up on you. and pouring at as low of a temperature as possible minimizes condensation a lot.

to get agar to melt again you have to go all the way back up to 85C, once it is gel simply heating it 47+ won't melt it.

Using more or less A-A in your recipe will make these temperatures and the stiffness of the agar plates a little bit different.
Everything here assumes 20g/L or 8g/400mL(20 plates worth)





https://www.shroomery.org/forums/showflat.php/Number/21922023

https://www.shroomery.org/forums/showflat.php/Number/22721954/vc/1#22721954

may as well do it the right way if you're going to do it


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Re: Not another Agar post. [Re: bodhisatta]
    #23800878 - 11/04/16 02:54 PM (7 years, 2 months ago)

I have that post of yours open for the lady few days lol very informative.


What if i don't have a pot small enough to boil 500ml?


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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23800990 - 11/04/16 03:42 PM (7 years, 2 months ago)

Put it all in the bottle and heat it up and shake it.


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Re: Not another Agar post. [Re: numnum59]
    #23800999 - 11/04/16 03:47 PM (7 years, 2 months ago)

I think you mean what if I don't have a pot big enough. you could boil 500mL in a soup stock pot if you wanted. just put it in a cup and microwave it to boiling


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Re: Not another Agar post. [Re: numnum59]
    #23801005 - 11/04/16 03:50 PM (7 years, 2 months ago)

Alright i did, heated it in microwave for 50 sec shook and sat in PC.


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There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23801018 - 11/04/16 03:56 PM (7 years, 2 months ago)

Quote:

TeaSippinHippie said:
Alright i did, heated it in microwave for 50 sec shook and sat in PC.




Keep in mind you can swirl it when you pull it out of the pc to mix it up. Just dont overthink it bro. Im out...  Good vibes to me ;]


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Re: Not another Agar post. [Re: numnum59]
    #23801027 - 11/04/16 03:59 PM (7 years, 2 months ago)

Thank you man,


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Re: Not another Agar post. [Re: bodhisatta]
    #23801057 - 11/04/16 04:09 PM (7 years, 2 months ago)

With it being so shallow?


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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23801110 - 11/04/16 04:25 PM (7 years, 2 months ago)

Quote:

TeaSippinHippie said:
Alright i did, heated it in microwave for 50 sec shook and sat in PC.




As long as you added it to hot water and stirred it or swirled it you should be fine.

I had 500ML in a pyrex measuring cup, put it in the microwave for 2 minutes and then stirred in my agar.

It's going to boil in the PC, so that should takecare of the rest of the mixing.


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Re: Not another Agar post. [Re: Spore Ninja]
    #23801125 - 11/04/16 04:29 PM (7 years, 2 months ago)

Using PC freaks me out. This thing sounds mad.


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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23801131 - 11/04/16 04:31 PM (7 years, 2 months ago)

Quote:

TeaSippinHippie said:
Using PC freaks me out. This thing sounds mad.




Is this your first time? Adjust the heat until steam is just escaping or the weight is just jiggling


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Re: Not another Agar post. [Re: numnum59]
    #23801207 - 11/04/16 04:58 PM (7 years, 2 months ago)

About my 6th time using one. But my grandmother had one blow up on her.


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There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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Re: Not another Agar post. [Re: numnum59]
    #23801211 - 11/04/16 05:00 PM (7 years, 2 months ago)

You are definitely over-thinking this.  Agar is easy as fuck


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Re: Not another Agar post. [Re: Kenetic]
    #23801218 - 11/04/16 05:03 PM (7 years, 2 months ago)

I'm sure ill think the same way after im done.

Do i need to wipe down the petris at all even though they come pre sterilized?


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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23801220 - 11/04/16 05:04 PM (7 years, 2 months ago)

no no no

Just wipe down the package and cut it open and remove the dishes.


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Re: Not another Agar post. [Re: Kenetic]
    #23801230 - 11/04/16 05:09 PM (7 years, 2 months ago)

Ok sweet :awedance:

I just took the PC off the eye, so ill be back in 3 hrs to do my first agar dishes.

Where do i need to place stuff, on the edges? I read countless times to not put your hand over anything. I have my glad bags, syringe, alcohol pads and dishes on the outer edges, ie back and sides


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There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23801270 - 11/04/16 05:24 PM (7 years, 2 months ago)

2 hours and 15 minutes at most


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Re: Not another Agar post. [Re: Kenetic]
    #23801515 - 11/04/16 06:45 PM (7 years, 2 months ago)

Its been almost 2 hours, so I'm going to go agead and move the agar to the SAB and clean everything with the 70% and open up the dishes.


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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23801609 - 11/04/16 07:17 PM (7 years, 2 months ago)

Agar came out of PC with small clump of something, i shook it and it dissolved.

Let the bottle cool in SAB till warm to touch and started pouring, i didn't have enough for all 20 so i must have poured a few to full, there was 5 left over. I put them in glad bags. The others i poured are hopefully solidifying


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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23801633 - 11/04/16 07:29 PM (7 years, 2 months ago)

You put them into bags immediately?
I let my plates sit in the closed up SAB until they set a good hour before putting them in the bags. You may have some sloping in your plates.

Whatever amount of agar works best for you, is the magic number. For me, I've been getting about 20plates out of a snapple bottle (which only really fits 400mL comfortably).


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Re: Not another Agar post. [Re: dankington]
    #23801636 - 11/04/16 07:32 PM (7 years, 2 months ago)

I put the 5 unused ones in bags, and the surface was slightly tilted but not much, after the 5th one i started correcting for the tilt.


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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23801685 - 11/04/16 07:55 PM (7 years, 2 months ago)

I am a Fan of a bit more agar in the plate. Of course you don't need much agar, but in my opinion more agar in one plate is easier to transfer later, because it's easier to "stab" into the agar wedge with the scalpel.

When I use very thin agar plates, I have the problem that I'm not able to "grab" the wedges for transfer and stabbing is also not that easy. So better use a bit more IMO.

Since agar and LME are the cheapest things in this hobby, you can afford it :P


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Re: Not another Agar post. [Re: AlCapone2k]
    #23801792 - 11/04/16 08:37 PM (7 years, 2 months ago)

How do i store these? The poured, un poured and the inoculated dishes?


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There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23801912 - 11/04/16 09:36 PM (7 years, 2 months ago)

Stacked in the sab.


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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23802696 - 11/05/16 08:11 AM (7 years, 2 months ago)

Quote:

TeaSippinHippie said:
I'm sure ill think the same way after im done.

Do i need to wipe down the petris at all even though they come pre sterilized?



Not unless you want to introduce contams


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Re: Not another Agar post. [Re: numnum59]
    #23802910 - 11/05/16 10:14 AM (7 years, 2 months ago)

Yeah i didn't do it dumb question, im filled with them.


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There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23803072 - 11/05/16 11:25 AM (7 years, 2 months ago)

Did you noc any of them up? After I poured, I hit 2 dishes with 2 drops each of a spore syringe solution. And I have the other plates ready to go when I need to make transfers in a couple of days. You want to hit the myc as soon as it starts to show- before a bunch of strain have formed and start fighting it out.


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Re: Not another Agar post. [Re: Spore Ninja]
    #23803211 - 11/05/16 12:17 PM (7 years, 2 months ago)

Yes i did 4 dishes, all were different amounts of agar so i can test the differences, and i also figured id contam 1 or 2. I also did 2 drops.

That leaves me with 11 poured plates.
I'm ordering 20 more Monday.

I'm happy that it gelled up, i figured I'd mess up so bad it wouldn't.


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MudaFuka said:
There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23806666 - 11/06/16 01:41 PM (7 years, 2 months ago)

Interesting tidbit from workman in a different agar thread:
Quote:

when you take foil wrapped glass dishes out of your PC the foil is going to be wet and I find it nicer to have everything including the package the dishes in dry so wrapping them in foil and baking them is preferred.




--------------------
I'm interested in Dr. Pollock's work and his (especially older) strains.
Anyone have pix of his Shroommobile RV??

PSA: Protonmail offers free, encrypted email hosted in privacy loving Switzerland.
Red Phone / Signal offer free encrypted phone calls and texting between users.

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Re: Not another Agar post. [Re: Spore Ninja]
    #23808222 - 11/06/16 10:18 PM (7 years, 2 months ago)

I was originally looking into the glass petris and bought some because i liked the oven idea, but after realizing I'll be going through a lot of dishes i put them away and bought plastic ones.

On that note no signs of mycelium or contam thats visible. All dishes cleared of condensation except a few of the extras i poured.


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There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23808775 - 11/07/16 07:24 AM (7 years, 2 months ago)

Sorry, wrong thread :smile:

But anyway thanks to enlightment for his answer :smile:


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Edited by AlCapone2k (11/07/16 08:30 AM)


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Re: Not another Agar post. [Re: AlCapone2k]
    #23808791 - 11/07/16 07:38 AM (7 years, 2 months ago)

:pm:

buy the cheaper ones if they are sold as 'sterile'.


Edited by enlightenment (11/07/16 07:45 AM)


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Re: Not another Agar post. [Re: enlightenment]
    #23809602 - 11/07/16 12:41 PM (7 years, 2 months ago)

Look into pastywhytes grocery store tek too


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Re: Not another Agar post. [Re: numnum59]
    #23813891 - 11/08/16 07:57 PM (7 years, 2 months ago)



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There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23813903 - 11/08/16 08:02 PM (7 years, 2 months ago)

Why do you have transpore tape wrapping your plate diametrically?
Why do you have Transpore tape at all?
Just axin' :popcorn:

got parafilm or sumthin'?


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Re: Not another Agar post. [Re: dankington]
    #23813924 - 11/08/16 08:08 PM (7 years, 2 months ago)

Quick question, hope I'm not hijacking anything.

About to inoculate some PC'd miniround agar plates (again). I am good at that part, but I can't find a solid post saying how to store these. Do you store them on a shelf, with the poretape AND paper towel on them? ziplock bag? heh thanks guys..


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Re: Not another Agar post. [Re: Levster]
    #23814799 - 11/09/16 12:43 AM (7 years, 2 months ago)

Looks like a tam broski


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Re: Not another Agar post. [Re: Levster]
    #23814801 - 11/09/16 12:44 AM (7 years, 2 months ago)

Quote:

Levster said:
Quick question, hope I'm not hijacking anything.

About to inoculate some PC'd miniround agar plates (again). I am good at that part, but I can't find a solid post saying how to store these. Do you store them on a shelf, with the poretape AND paper towel on them? ziplock bag? heh thanks guys..




Store them in the w fridge how you removed them from the pc, foil with paper towel over them


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Re: Not another Agar post. [Re: numnum59]
    #23815481 - 11/09/16 08:14 AM (7 years, 2 months ago)

just leave them on a shelf, counter, tote, anywhere and wait for the condensation to go away (if you dont like condensation on the walls)


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Re: Not another Agar post. [Re: spacechildo]
    #23815823 - 11/09/16 10:28 AM (7 years, 2 months ago)

I keep mine in a clean tote or fresh trash bag until needed just for better protection from dust and what not.
just for peace of mind


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Re: Not another Agar post. [Re: Levster]
    #23816291 - 11/09/16 01:46 PM (7 years, 2 months ago)

Quote:

Levster said:
Quick question, hope I'm not hijacking anything.

About to inoculate some PC'd miniround agar plates (again). I am good at that part, but I can't find a solid post saying how to store these. Do you store them on a shelf, with the poretape AND paper towel on them? ziplock bag? heh thanks guys..





Before Inoc: back inside the plastics sleeve the dishes came in.
after inoc: inside a ziplock, inside the SAB or another clear rubermaid/sterilite tub.


--------------------
I'm interested in Dr. Pollock's work and his (especially older) strains.
Anyone have pix of his Shroommobile RV??

PSA: Protonmail offers free, encrypted email hosted in privacy loving Switzerland.
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Re: Not another Agar post. [Re: Spore Ninja]
    #23816445 - 11/09/16 02:39 PM (7 years, 2 months ago)

mini rounds doesnt come in a sleeve, petris does.
just leave them on a counter,shelf etc. paper on is fine.


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Re: Not another Agar post. [Re: spacechildo]
    #23816624 - 11/09/16 03:43 PM (7 years, 2 months ago)

I didn't want the lids coming off in the bags so i tapped them.

I feel my first try at agar was a failure, since all I've gotten is that 1 white dot, makes me want to toss the others i poured.

guess i need to order more dishes.


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MudaFuka said:
There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23816666 - 11/09/16 03:54 PM (7 years, 2 months ago)

after inoculation you need to wrap the dishes man. so the 2 pieces stick together and the crack between them are covered.
why dont you just follow the tek instead of trying to think up stuff yourself until you get some experience?


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Re: Not another Agar post. [Re: spacechildo]
    #23816754 - 11/09/16 04:18 PM (7 years, 2 months ago)

Man were trying to give you sound advice but your not listening to us.


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Re: Not another Agar post. [Re: numnum59]
    #23816857 - 11/09/16 04:46 PM (7 years, 2 months ago)

I thought someone on here said they just put them in glad bags no wrap. My bad. I had the wrap, ill use it next time.


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MudaFuka said:
There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23817035 - 11/09/16 05:52 PM (7 years, 2 months ago)

I think they were talking about petris :wink:


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Re: Not another Agar post. [Re: AlCapone2k]
    #23817528 - 11/09/16 08:11 PM (7 years, 2 months ago)

Im using petri dishes, but from now on ill wrap them before putting them in bags.


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MudaFuka said:
There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23817664 - 11/09/16 09:09 PM (7 years, 2 months ago)

Quote:

TeaSippinHippie said:
Im using petri dishes, but from now on ill wrap them before putting them in bags.




Man my bad i completely missed the fucking bag. It looks good how you did itbut that looks like a tam


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Re: Not another Agar post. [Re: numnum59]
    #23818489 - 11/10/16 06:36 AM (7 years, 2 months ago)

Yes it looked like a small donut with a blie dot in the middle, i tossed it.

The 4 others haven't shown any signs of life. I'm blaming it on not boiling the agar first and just popping them in the PC.


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MudaFuka said:
There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23818658 - 11/10/16 08:02 AM (7 years, 2 months ago)

Lol I'm sure boiling has absolutely nothing to.do with it


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Re: Not another Agar post. [Re: Kenetic]
    #23818739 - 11/10/16 08:39 AM (7 years, 2 months ago)

No, not with his problem. Still I'd recommend to boil at least LME Agar before putting it into whatever to PC it. It's quite important to get a clear liquid :smile:


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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23818745 - 11/10/16 08:42 AM (7 years, 2 months ago)

Quote:

TeaSippinHippie said:
Yes it looked like a small donut with a blie dot in the middle, i tossed it.

The 4 others haven't shown any signs of life. I'm blaming it on not boiling the agar first and just popping them in the PC.




start worrying in 2 weeks or so, dont make those weird assumptions that doesnt make any sense, it will just confuse you.
spores need time to germinate. 3 days to 3 weeks depending on their age IME.

ALcapone; sediments in the agar never bothered me nor has it given me any problems. especially not with germinating spores.


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Re: Not another Agar post. [Re: spacechildo]
    #23818767 - 11/10/16 08:51 AM (7 years, 2 months ago)

Quote:

spacechildo said:
ALcapone; sediments in the agar never bothered me nor has it given me any problems. especially not with germinating spores.






you are absolutely right, it doesn't cause any problems, but sometimes it could lead to misinterpretations of plates, like seeing contamns but it's just a residue. Especially for noobs like me sometimes confusing. And sometimes when you don't boil your agar enough you'll get "hilly" agar plates, that also doesn't cause any problem but doesn't look good and makes it harde to identify differen mycelium growth speeds


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Re: Not another Agar post. [Re: AlCapone2k]
    #23818803 - 11/10/16 09:02 AM (7 years, 2 months ago)

what's a hilly agar plate? sediments always settle in my plates, as I said I've never had a problem in any way with a few darker spots on the plate, you can clearly see its not something that grows on your plate.


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Re: Not another Agar post. [Re: spacechildo]
    #23818944 - 11/10/16 09:35 AM (7 years, 2 months ago)

Hilly agarplates occur, when you pour the agar into the petris and before the bottle is empty the last agar drops have cooled down a bit to much so they are more solid. When pouring it into the plate, you get some "hills" or "mountains" and not a totally plain agar in the plate.

Sorry for my english, I hope I am able to explain what I want to say with my german school english :laugh:


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Re: Not another Agar post. [Re: AlCapone2k]
    #23818949 - 11/10/16 09:37 AM (7 years, 2 months ago)

so you say cooled agar can cause "hilly plates" but what does that have to do with how you prepped it before PC'ing?


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Re: Not another Agar post. [Re: spacechildo]
    #23818973 - 11/10/16 09:42 AM (7 years, 2 months ago)

Actually I don't know the reason for this "Hilly plates", could be the cooling, could be the solution of the ingredients before PCing. I don't know. Since I'm using bhodisattas pour agar tek I saked him if he knows why sometimes I got these kind of plates.

It was just an idea, that it could be caused by not soluting the agar agar + LME well before PCing.


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Re: Not another Agar post. [Re: AlCapone2k]
    #23818988 - 11/10/16 09:45 AM (7 years, 2 months ago)

Quote:

AlCapone2k said:
It's quite important to get a clear liquid :smile:




No, it's not


--------------------
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Re: Not another Agar post. [Re: Kenetic]
    #23819000 - 11/10/16 09:48 AM (7 years, 2 months ago)

I have the answer from bhodi:
Quote:

bodhisatta said:
You may be having some agar get solidified while it's cooling down in the bottle. You have to swirl / mix the bottle now and then so that the temperature stays uniform otherwise youll get some agar solidified because it gets cold faster in some parts of the bottle. Or use enough water in the water bath to go over the level of agar in the bottle

You could try 52C too just a hair hotter





The reason seems to be the cooling process, not the cooking before. Sorry guys :smile:


Quote:

kenetic said:
Quote:

AlCapone2k said:
It's quite important to get a clear liquid :smile:




No, it's not





As I said, it's not important for myc grwoth or contamination or something. But a clear solution of the ingredients is better because then you get a clean agar plate without any confusion that some residue could be a contamination or something.


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Re: Not another Agar post. [Re: AlCapone2k]
    #23819057 - 11/10/16 10:02 AM (7 years, 2 months ago)

None of my un knocked up plates i poured have contam that i can see, just that one i did inoculate. So i guess thats a good sign.

Normally i see life by now in bags from MS. Either way im putting this try up and doing another batch. I'll check on these periodically.

Thanks for all the help through this guys, even the discussions im not in are helpful haha.


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Re: Not another Agar post. [Re: AlCapone2k]
    #23819087 - 11/10/16 10:09 AM (7 years, 2 months ago)

Quote:

AlCapone2k said:
As I said, it's not important for myc grwoth or contamination or something. But a clear solution of the ingredients is better because then you get a clean agar plate without any confusion that some residue could be a contamination or something.




have you done this yourself (confused sediments for contams) or are you repeating what you've heard someone say?
it seems pretty far fetched to me.


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Re: Not another Agar post. [Re: spacechildo]
    #23819103 - 11/10/16 10:14 AM (7 years, 2 months ago)

I vastly prefer clear agar. Opaqueness isn't ideal by any means. Also if it's clear that means everything dissolved there's no sediment. Clear totally dissolved media is easier to sterilize as well


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Re: Not another Agar post. [Re: spacechildo]
    #23819111 - 11/10/16 10:16 AM (7 years, 2 months ago)

All of my agar contams have been pretty obvious (to me) and my agar generally has a little sediment.  With that said, I could see how clear agar may help someone spot contains easier, but clear agar is not going to affect growth. I just want to make that clear.
I've seen people use black agar actually.

Edit:  too much sediment is obviously a problem, as stated above


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Re: Not another Agar post. [Re: bodhisatta]
    #23819134 - 11/10/16 10:22 AM (7 years, 2 months ago)

Quote:

bodhisatta said:
I vastly prefer clear agar. Opaqueness isn't ideal by any means. Also if it's clear that means everything dissolved there's no sediment. Clear totally dissolved media is easier to sterilize as well




but have you ever confused sediments for contams?

or had problems because sediments made sterilization harder/incomplete?

visibility through the bottom of the petris I can agree with, but not all those other points being brought up earlier in this thread.


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Re: Not another Agar post. [Re: spacechildo]
    #23819158 - 11/10/16 10:27 AM (7 years, 2 months ago)

I use brf agar all the time. It's cloudier than jizz lemonade
But clear agar does have its place


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OfflineMushierage
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Re: Not another Agar post. [Re: bodhisatta]
    #23819177 - 11/10/16 10:32 AM (7 years, 2 months ago)

I just use mea.  The cultures I have seem to like mea a lot so I don't bother with anything else, though I have wanted to try changing up medium for isolation purposes.


--------------------
Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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OfflineAlCapone2k
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Re: Not another Agar post. [Re: spacechildo]
    #23819211 - 11/10/16 10:43 AM (7 years, 2 months ago)

Quote:

spacechildo said:
Quote:

AlCapone2k said:
As I said, it's not important for myc grwoth or contamination or something. But a clear solution of the ingredients is better because then you get a clean agar plate without any confusion that some residue could be a contamination or something.




have you done this yourself (confused sediments for contams) or are you repeating what you've heard someone say?
it seems pretty far fetched to me.






No, I don't repeat anything heared someone say. I was in the same situation.

I asked the question myself in the Q and A Noob Thread. Here:

https://www.shroomery.org/forums/showflat.php/Number/23751106#23751106


So I think my point seems legit. It by no means any offence to your opinion.


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


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InvisiblebodhisattaMDiscordReddit
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Re: Not another Agar post. [Re: Mushierage]
    #23819247 - 11/10/16 10:51 AM (7 years, 2 months ago)

Quote:

Mushierage said:
I just use mea.  The cultures I have seem to like mea a lot so I don't bother with anything else, though I have wanted to try changing up medium for isolation purposes.



DIY mea is cloudy usually unless filtered. If you buy mea it will be crystal clear


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Offlinespacechildo
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Re: Not another Agar post. [Re: AlCapone2k]
    #23819298 - 11/10/16 11:09 AM (7 years, 2 months ago)

Quote:

AlCapone2k said:
Quote:

spacechildo said:
Quote:

AlCapone2k said:
As I said, it's not important for myc grwoth or contamination or something. But a clear solution of the ingredients is better because then you get a clean agar plate without any confusion that some residue could be a contamination or something.




have you done this yourself (confused sediments for contams) or are you repeating what you've heard someone say?
it seems pretty far fetched to me.






No, I don't repeat anything heared someone say. I was in the same situation.

I asked the question myself in the Q and A Noob Thread. Here:

https://www.shroomery.org/forums/showflat.php/Number/23751106#23751106


So I think my point seems legit. It by no means any offence to your opinion.




:thumbup:
no offence taken, and no offence meant, it just seemed far fetched to confuse sediments for contams to me but there ya go!


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OfflineMushierage
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Re: Not another Agar post. [Re: bodhisatta]
    #23819734 - 11/10/16 12:52 PM (7 years, 2 months ago)

I use premixed mea.  It's pretty darn clear, and can be mixed with any type of food coloring if needed.


--------------------
Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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Offlinenumnum59
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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23819817 - 11/10/16 01:18 PM (7 years, 2 months ago)

Quote:

TeaSippinHippie said:
Yes it looked like a small donut with a blie dot in the middle, i tossed it.

The 4 others haven't shown any signs of life. I'm blaming it on not boiling the agar first and just popping them in the PC.




Did u go from multi spore homie? If so dont expect fast growth immediately. Even with lc or mushroom tissue it takes a few days for it to figure out what to do with the agar lol


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OfflineSpore Ninja
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Re: Not another Agar post. [Re: spacechildo]
    #23821544 - 11/10/16 11:40 PM (7 years, 2 months ago)

Quote:

spacechildo said:
mini rounds doesnt come in a sleeve, petris does.
just leave them on a counter,shelf etc. paper on is fine.



Oops- he said "plates" I thought he meant the 60MM petris.

(that's assuming I deciphered your sentence correctly)
They don't think it be like it is
But it do.


--------------------
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OfflineSpore Ninja
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Re: Not another Agar post. [Re: AlCapone2k]
    #23821575 - 11/10/16 11:53 PM (7 years, 2 months ago)

Quote:

AlCapone2k said:
I have the answer from bhodi:
Quote:

bodhisatta said:
You may be having some agar get solidified while it's cooling down in the bottle. You have to swirl / mix the bottle now and then so that the temperature stays uniform otherwise youll get some agar solidified because it gets cold faster in some parts of the bottle. Or use enough water in the water bath to go over the level of agar in the bottle

You could try 52C too just a hair hotter





The reason seems to be the cooling process, not the cooking before. Sorry guys :smile:


Quote:

kenetic said:
Quote:

AlCapone2k said:
It's quite important to get a clear liquid :smile:




No, it's not





As I said, it's not important for myc grwoth or contamination or something. But a clear solution of the ingredients is better because then you get a clean agar plate without any confusion that some residue could be a contamination or something.





Mine is black.

Am I screwed?


--------------------
I'm interested in Dr. Pollock's work and his (especially older) strains.
Anyone have pix of his Shroommobile RV??

PSA: Protonmail offers free, encrypted email hosted in privacy loving Switzerland.
Red Phone / Signal offer free encrypted phone calls and texting between users.

All of these programs are available free for PC and Android or apple and are available in the respective stores or online by googling the names.
Stay safe out there!

The Electronic Freedom Foundation's review of text and messaging program's security is here>>> https://www.eff.org/node/82654


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OfflineAlCapone2k
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Re: Not another Agar post. [Re: Spore Ninja]
    #23822073 - 11/11/16 06:36 AM (7 years, 2 months ago)

You mean your agar inside the plate is black? Did you use LME-Agar or any food coloring?


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


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OfflineSpore Ninja
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Re: Not another Agar post. [Re: AlCapone2k]
    #23824062 - 11/11/16 08:23 PM (7 years, 2 months ago)

DFA and AC.


--------------------
I'm interested in Dr. Pollock's work and his (especially older) strains.
Anyone have pix of his Shroommobile RV??

PSA: Protonmail offers free, encrypted email hosted in privacy loving Switzerland.
Red Phone / Signal offer free encrypted phone calls and texting between users.

All of these programs are available free for PC and Android or apple and are available in the respective stores or online by googling the names.
Stay safe out there!

The Electronic Freedom Foundation's review of text and messaging program's security is here>>> https://www.eff.org/node/82654


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Re: Not another Agar post. [Re: Spore Ninja]
    #23824067 - 11/11/16 08:27 PM (7 years, 2 months ago)

Think ya burnt it.


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OfflinePitviper
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Re: Not another Agar post. [Re: Spore Ninja]
    #23824206 - 11/11/16 09:46 PM (7 years, 2 months ago)

You can buy nichrome wire from vapeshops for like 5 bucks a spool.


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Offlinenumnum59
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Re: Not another Agar post. [Re: Pitviper]
    #23825398 - 11/12/16 09:47 AM (7 years, 2 months ago)

Your agar is black? What recipe did u follow?


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OfflineTeaSippinHippie
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Re: Not another Agar post. [Re: numnum59]
    #23837834 - 11/16/16 06:42 AM (7 years, 2 months ago)

Got life on all the remaining dishes, mycelium is a little on the fluffy side.


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I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE.
MudaFuka said:
There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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OfflineSpore Ninja
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Re: Not another Agar post. [Re: Pitviper]
    #23845246 - 11/18/16 01:23 PM (7 years, 2 months ago)

Quote:

Pitviper said:
You can buy nichrome wire from vapeshops for like 5 bucks a spool.




Very interesting idea... Thanks.


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OfflineSpore Ninja
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Re: Not another Agar post. [Re: numnum59]
    #23845249 - 11/18/16 01:26 PM (7 years, 2 months ago)

Quote:

numnum59 said:
Your agar is black? What recipe did u follow?




The agar is fine. And the white ropey myc looks sexy AF against the black background.


--------------------
I'm interested in Dr. Pollock's work and his (especially older) strains.
Anyone have pix of his Shroommobile RV??

PSA: Protonmail offers free, encrypted email hosted in privacy loving Switzerland.
Red Phone / Signal offer free encrypted phone calls and texting between users.

All of these programs are available free for PC and Android or apple and are available in the respective stores or online by googling the names.
Stay safe out there!

The Electronic Freedom Foundation's review of text and messaging program's security is here>>> https://www.eff.org/node/82654


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OfflineTeaSippinHippie
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Re: Not another Agar post. [Re: Spore Ninja]
    #23849016 - 11/19/16 03:41 PM (7 years, 2 months ago)



--------------------
I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE.
MudaFuka said:
There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23854206 - 11/21/16 12:40 PM (7 years, 2 months ago)

Quote:

TeaSippinHippie said:





Nice! :wink:

Now whatcha gonna do with it??


--------------------
I'm interested in Dr. Pollock's work and his (especially older) strains.
Anyone have pix of his Shroommobile RV??

PSA: Protonmail offers free, encrypted email hosted in privacy loving Switzerland.
Red Phone / Signal offer free encrypted phone calls and texting between users.

All of these programs are available free for PC and Android or apple and are available in the respective stores or online by googling the names.
Stay safe out there!

The Electronic Freedom Foundation's review of text and messaging program's security is here>>> https://www.eff.org/node/82654


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OfflineTeaSippinHippie
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Re: Not another Agar post. [Re: Spore Ninja]
    #23858243 - 11/22/16 06:21 PM (7 years, 2 months ago)

Transfer it to another plate here soon.


--------------------
I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE.
MudaFuka said:
There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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Offlinespacechildo
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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23858274 - 11/22/16 06:30 PM (7 years, 2 months ago)

i'd just take a tiny tiny piece of the circle to the most left, around 10-11 o clock.
there's a lot going on on that plate.


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OfflineSpore Ninja
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Re: Not another Agar post. [Re: spacechildo]
    #23860695 - 11/23/16 01:33 PM (7 years, 2 months ago)

Quote:

spacechildo said:
i'd just take a tiny tiny piece of the circle to the most left, around 10-11 o clock.
there's a lot going on on that plate.




That looks like the best area... I'd be tempted to take 5 or more tiny cuts from that area, and then transfer them to new dishes and see what you get.


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