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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Not another Agar post. [Re: AlCapone2k]
#23818803 - 11/10/16 09:02 AM (7 years, 2 months ago) |
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what's a hilly agar plate? sediments always settle in my plates, as I said I've never had a problem in any way with a few darker spots on the plate, you can clearly see its not something that grows on your plate.
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Hilly agarplates occur, when you pour the agar into the petris and before the bottle is empty the last agar drops have cooled down a bit to much so they are more solid. When pouring it into the plate, you get some "hills" or "mountains" and not a totally plain agar in the plate.
Sorry for my english, I hope I am able to explain what I want to say with my german school english
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Not another Agar post. [Re: AlCapone2k]
#23818949 - 11/10/16 09:37 AM (7 years, 2 months ago) |
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so you say cooled agar can cause "hilly plates" but what does that have to do with how you prepped it before PC'ing?
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Actually I don't know the reason for this "Hilly plates", could be the cooling, could be the solution of the ingredients before PCing. I don't know. Since I'm using bhodisattas pour agar tek I saked him if he knows why sometimes I got these kind of plates.
It was just an idea, that it could be caused by not soluting the agar agar + LME well before PCing.
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
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Re: Not another Agar post. [Re: AlCapone2k]
#23818988 - 11/10/16 09:45 AM (7 years, 2 months ago) |
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Quote:
AlCapone2k said: It's quite important to get a clear liquid 
No, it's not
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Not another Agar post. [Re: Kenetic]
#23819000 - 11/10/16 09:48 AM (7 years, 2 months ago) |
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I have the answer from bhodi: Quote:
bodhisatta said: You may be having some agar get solidified while it's cooling down in the bottle. You have to swirl / mix the bottle now and then so that the temperature stays uniform otherwise youll get some agar solidified because it gets cold faster in some parts of the bottle. Or use enough water in the water bath to go over the level of agar in the bottle
You could try 52C too just a hair hotter
The reason seems to be the cooling process, not the cooking before. Sorry guys
Quote:
kenetic said:
Quote:
AlCapone2k said: It's quite important to get a clear liquid 
No, it's not
As I said, it's not important for myc grwoth or contamination or something. But a clear solution of the ingredients is better because then you get a clean agar plate without any confusion that some residue could be a contamination or something.
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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TeaSippinHippie



Registered: 11/09/13
Posts: 621
Loc: small sticks town
Last seen: 3 years, 2 months
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Re: Not another Agar post. [Re: AlCapone2k]
#23819057 - 11/10/16 10:02 AM (7 years, 2 months ago) |
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None of my un knocked up plates i poured have contam that i can see, just that one i did inoculate. So i guess thats a good sign.
Normally i see life by now in bags from MS. Either way im putting this try up and doing another batch. I'll check on these periodically.
Thanks for all the help through this guys, even the discussions im not in are helpful haha.
-------------------- I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE. MudaFuka said: There's nothing wrong with making mistakes as long as you don't make the same ones twice.
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Not another Agar post. [Re: AlCapone2k]
#23819087 - 11/10/16 10:09 AM (7 years, 2 months ago) |
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Quote:
AlCapone2k said: As I said, it's not important for myc grwoth or contamination or something. But a clear solution of the ingredients is better because then you get a clean agar plate without any confusion that some residue could be a contamination or something.
have you done this yourself (confused sediments for contams) or are you repeating what you've heard someone say? it seems pretty far fetched to me.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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I vastly prefer clear agar. Opaqueness isn't ideal by any means. Also if it's clear that means everything dissolved there's no sediment. Clear totally dissolved media is easier to sterilize as well
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
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All of my agar contams have been pretty obvious (to me) and my agar generally has a little sediment. With that said, I could see how clear agar may help someone spot contains easier, but clear agar is not going to affect growth. I just want to make that clear. I've seen people use black agar actually.
Edit: too much sediment is obviously a problem, as stated above
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Not another Agar post. [Re: bodhisatta]
#23819134 - 11/10/16 10:22 AM (7 years, 2 months ago) |
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Quote:
bodhisatta said: I vastly prefer clear agar. Opaqueness isn't ideal by any means. Also if it's clear that means everything dissolved there's no sediment. Clear totally dissolved media is easier to sterilize as well
but have you ever confused sediments for contams?
or had problems because sediments made sterilization harder/incomplete?
visibility through the bottom of the petris I can agree with, but not all those other points being brought up earlier in this thread.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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I use brf agar all the time. It's cloudier than jizz lemonade But clear agar does have its place
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Mushierage
SWIM Sinker



Registered: 06/29/16
Posts: 1,094
Last seen: 7 years, 1 month
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Re: Not another Agar post. [Re: bodhisatta]
#23819177 - 11/10/16 10:32 AM (7 years, 2 months ago) |
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I just use mea. The cultures I have seem to like mea a lot so I don't bother with anything else, though I have wanted to try changing up medium for isolation purposes.
-------------------- Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup. OR... You should be cultivating reptiles and fish, not mushrooms.
  
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Quote:
spacechildo said:
Quote:
AlCapone2k said: As I said, it's not important for myc grwoth or contamination or something. But a clear solution of the ingredients is better because then you get a clean agar plate without any confusion that some residue could be a contamination or something.
have you done this yourself (confused sediments for contams) or are you repeating what you've heard someone say? it seems pretty far fetched to me.
No, I don't repeat anything heared someone say. I was in the same situation.
I asked the question myself in the Q and A Noob Thread. Here:
https://www.shroomery.org/forums/showflat.php/Number/23751106#23751106
So I think my point seems legit. It by no means any offence to your opinion.
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Not another Agar post. [Re: Mushierage]
#23819247 - 11/10/16 10:51 AM (7 years, 2 months ago) |
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Quote:
Mushierage said: I just use mea. The cultures I have seem to like mea a lot so I don't bother with anything else, though I have wanted to try changing up medium for isolation purposes.
DIY mea is cloudy usually unless filtered. If you buy mea it will be crystal clear
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Not another Agar post. [Re: AlCapone2k]
#23819298 - 11/10/16 11:09 AM (7 years, 2 months ago) |
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Quote:
AlCapone2k said:
Quote:
spacechildo said:
Quote:
AlCapone2k said: As I said, it's not important for myc grwoth or contamination or something. But a clear solution of the ingredients is better because then you get a clean agar plate without any confusion that some residue could be a contamination or something.
have you done this yourself (confused sediments for contams) or are you repeating what you've heard someone say? it seems pretty far fetched to me.
No, I don't repeat anything heared someone say. I was in the same situation.
I asked the question myself in the Q and A Noob Thread. Here:
https://www.shroomery.org/forums/showflat.php/Number/23751106#23751106
So I think my point seems legit. It by no means any offence to your opinion.
 no offence taken, and no offence meant, it just seemed far fetched to confuse sediments for contams to me but there ya go!
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Mushierage
SWIM Sinker



Registered: 06/29/16
Posts: 1,094
Last seen: 7 years, 1 month
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Re: Not another Agar post. [Re: bodhisatta]
#23819734 - 11/10/16 12:52 PM (7 years, 2 months ago) |
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I use premixed mea. It's pretty darn clear, and can be mixed with any type of food coloring if needed.
-------------------- Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup. OR... You should be cultivating reptiles and fish, not mushrooms.
  
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numnum59
Pro-Am Mycologist


Registered: 12/07/11
Posts: 1,189
Last seen: 5 months, 22 days
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Quote:
TeaSippinHippie said: Yes it looked like a small donut with a blie dot in the middle, i tossed it.
The 4 others haven't shown any signs of life. I'm blaming it on not boiling the agar first and just popping them in the PC.
Did u go from multi spore homie? If so dont expect fast growth immediately. Even with lc or mushroom tissue it takes a few days for it to figure out what to do with the agar lol
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Spore Ninja
PsychoMycoPhile



Registered: 10/25/16
Posts: 161
Last seen: 5 years, 6 months
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Quote:
spacechildo said: mini rounds doesnt come in a sleeve, petris does. just leave them on a counter,shelf etc. paper on is fine.
Oops- he said "plates" I thought he meant the 60MM petris.
(that's assuming I deciphered your sentence correctly) They don't think it be like it is But it do.
-------------------- I'm interested in Dr. Pollock's work and his (especially older) strains. Anyone have pix of his Shroommobile RV?? PSA: Protonmail offers free, encrypted email hosted in privacy loving Switzerland. Red Phone / Signal offer free encrypted phone calls and texting between users. All of these programs are available free for PC and Android or apple and are available in the respective stores or online by googling the names. Stay safe out there! The Electronic Freedom Foundation's review of text and messaging program's security is here>>> https://www.eff.org/node/82654
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Spore Ninja
PsychoMycoPhile



Registered: 10/25/16
Posts: 161
Last seen: 5 years, 6 months
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Re: Not another Agar post. [Re: AlCapone2k]
#23821575 - 11/10/16 11:53 PM (7 years, 2 months ago) |
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Quote:
AlCapone2k said: I have the answer from bhodi: Quote:
bodhisatta said: You may be having some agar get solidified while it's cooling down in the bottle. You have to swirl / mix the bottle now and then so that the temperature stays uniform otherwise youll get some agar solidified because it gets cold faster in some parts of the bottle. Or use enough water in the water bath to go over the level of agar in the bottle
You could try 52C too just a hair hotter
The reason seems to be the cooling process, not the cooking before. Sorry guys
Quote:
kenetic said:
Quote:
AlCapone2k said: It's quite important to get a clear liquid 
No, it's not
As I said, it's not important for myc grwoth or contamination or something. But a clear solution of the ingredients is better because then you get a clean agar plate without any confusion that some residue could be a contamination or something.
Mine is black.
Am I screwed?
-------------------- I'm interested in Dr. Pollock's work and his (especially older) strains. Anyone have pix of his Shroommobile RV?? PSA: Protonmail offers free, encrypted email hosted in privacy loving Switzerland. Red Phone / Signal offer free encrypted phone calls and texting between users. All of these programs are available free for PC and Android or apple and are available in the respective stores or online by googling the names. Stay safe out there! The Electronic Freedom Foundation's review of text and messaging program's security is here>>> https://www.eff.org/node/82654
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