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Invisibledankington
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Registered: 03/14/15
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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23813903 - 11/08/16 08:02 PM (7 years, 2 months ago)

Why do you have transpore tape wrapping your plate diametrically?
Why do you have Transpore tape at all?
Just axin' :popcorn:

got parafilm or sumthin'?


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OfflineLevster
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Registered: 08/17/16
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Re: Not another Agar post. [Re: dankington]
    #23813924 - 11/08/16 08:08 PM (7 years, 2 months ago)

Quick question, hope I'm not hijacking anything.

About to inoculate some PC'd miniround agar plates (again). I am good at that part, but I can't find a solid post saying how to store these. Do you store them on a shelf, with the poretape AND paper towel on them? ziplock bag? heh thanks guys..


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Offlinenumnum59
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Re: Not another Agar post. [Re: Levster]
    #23814799 - 11/09/16 12:43 AM (7 years, 2 months ago)

Looks like a tam broski


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Offlinenumnum59
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Re: Not another Agar post. [Re: Levster]
    #23814801 - 11/09/16 12:44 AM (7 years, 2 months ago)

Quote:

Levster said:
Quick question, hope I'm not hijacking anything.

About to inoculate some PC'd miniround agar plates (again). I am good at that part, but I can't find a solid post saying how to store these. Do you store them on a shelf, with the poretape AND paper towel on them? ziplock bag? heh thanks guys..




Store them in the w fridge how you removed them from the pc, foil with paper towel over them


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Offlinespacechildo
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Re: Not another Agar post. [Re: numnum59]
    #23815481 - 11/09/16 08:14 AM (7 years, 2 months ago)

just leave them on a shelf, counter, tote, anywhere and wait for the condensation to go away (if you dont like condensation on the walls)


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Offlinetombosley8
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Re: Not another Agar post. [Re: spacechildo]
    #23815823 - 11/09/16 10:28 AM (7 years, 2 months ago)

I keep mine in a clean tote or fresh trash bag until needed just for better protection from dust and what not.
just for peace of mind


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OfflineSpore Ninja
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Re: Not another Agar post. [Re: Levster]
    #23816291 - 11/09/16 01:46 PM (7 years, 2 months ago)

Quote:

Levster said:
Quick question, hope I'm not hijacking anything.

About to inoculate some PC'd miniround agar plates (again). I am good at that part, but I can't find a solid post saying how to store these. Do you store them on a shelf, with the poretape AND paper towel on them? ziplock bag? heh thanks guys..





Before Inoc: back inside the plastics sleeve the dishes came in.
after inoc: inside a ziplock, inside the SAB or another clear rubermaid/sterilite tub.


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I'm interested in Dr. Pollock's work and his (especially older) strains.
Anyone have pix of his Shroommobile RV??

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Offlinespacechildo
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Re: Not another Agar post. [Re: Spore Ninja]
    #23816445 - 11/09/16 02:39 PM (7 years, 2 months ago)

mini rounds doesnt come in a sleeve, petris does.
just leave them on a counter,shelf etc. paper on is fine.


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OfflineTeaSippinHippie
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Re: Not another Agar post. [Re: spacechildo]
    #23816624 - 11/09/16 03:43 PM (7 years, 2 months ago)

I didn't want the lids coming off in the bags so i tapped them.

I feel my first try at agar was a failure, since all I've gotten is that 1 white dot, makes me want to toss the others i poured.

guess i need to order more dishes.


--------------------
I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE.
MudaFuka said:
There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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Offlinespacechildo
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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23816666 - 11/09/16 03:54 PM (7 years, 2 months ago)

after inoculation you need to wrap the dishes man. so the 2 pieces stick together and the crack between them are covered.
why dont you just follow the tek instead of trying to think up stuff yourself until you get some experience?


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Offlinenumnum59
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Re: Not another Agar post. [Re: spacechildo]
    #23816754 - 11/09/16 04:18 PM (7 years, 2 months ago)

Man were trying to give you sound advice but your not listening to us.


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OfflineTeaSippinHippie
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Re: Not another Agar post. [Re: numnum59]
    #23816857 - 11/09/16 04:46 PM (7 years, 2 months ago)

I thought someone on here said they just put them in glad bags no wrap. My bad. I had the wrap, ill use it next time.


--------------------
I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE.
MudaFuka said:
There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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OfflineAlCapone2k
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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23817035 - 11/09/16 05:52 PM (7 years, 2 months ago)

I think they were talking about petris :wink:


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Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


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OfflineTeaSippinHippie
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Re: Not another Agar post. [Re: AlCapone2k]
    #23817528 - 11/09/16 08:11 PM (7 years, 2 months ago)

Im using petri dishes, but from now on ill wrap them before putting them in bags.


--------------------
I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE.
MudaFuka said:
There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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Offlinenumnum59
Pro-Am Mycologist


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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23817664 - 11/09/16 09:09 PM (7 years, 2 months ago)

Quote:

TeaSippinHippie said:
Im using petri dishes, but from now on ill wrap them before putting them in bags.




Man my bad i completely missed the fucking bag. It looks good how you did itbut that looks like a tam


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OfflineTeaSippinHippie
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Re: Not another Agar post. [Re: numnum59]
    #23818489 - 11/10/16 06:36 AM (7 years, 2 months ago)

Yes it looked like a small donut with a blie dot in the middle, i tossed it.

The 4 others haven't shown any signs of life. I'm blaming it on not boiling the agar first and just popping them in the PC.


--------------------
I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE.
MudaFuka said:
There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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OfflineKenetic
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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23818658 - 11/10/16 08:02 AM (7 years, 2 months ago)

Lol I'm sure boiling has absolutely nothing to.do with it


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Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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OfflineAlCapone2k
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Re: Not another Agar post. [Re: Kenetic]
    #23818739 - 11/10/16 08:39 AM (7 years, 2 months ago)

No, not with his problem. Still I'd recommend to boil at least LME Agar before putting it into whatever to PC it. It's quite important to get a clear liquid :smile:


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


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Offlinespacechildo
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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23818745 - 11/10/16 08:42 AM (7 years, 2 months ago)

Quote:

TeaSippinHippie said:
Yes it looked like a small donut with a blie dot in the middle, i tossed it.

The 4 others haven't shown any signs of life. I'm blaming it on not boiling the agar first and just popping them in the PC.




start worrying in 2 weeks or so, dont make those weird assumptions that doesnt make any sense, it will just confuse you.
spores need time to germinate. 3 days to 3 weeks depending on their age IME.

ALcapone; sediments in the agar never bothered me nor has it given me any problems. especially not with germinating spores.


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OfflineAlCapone2k
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Re: Not another Agar post. [Re: spacechildo]
    #23818767 - 11/10/16 08:51 AM (7 years, 2 months ago)

Quote:

spacechildo said:
ALcapone; sediments in the agar never bothered me nor has it given me any problems. especially not with germinating spores.






you are absolutely right, it doesn't cause any problems, but sometimes it could lead to misinterpretations of plates, like seeing contamns but it's just a residue. Especially for noobs like me sometimes confusing. And sometimes when you don't boil your agar enough you'll get "hilly" agar plates, that also doesn't cause any problem but doesn't look good and makes it harde to identify differen mycelium growth speeds


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


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Shop: PhytoExtractum Kratom Powder for Sale   Bridgetown Botanicals CBD Concentrates   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags   Unfolding Nature Unfolding Nature: Being in the Implicate Order


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