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OfflineKenetic
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Registered: 08/24/14
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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23801220 - 11/04/16 05:04 PM (7 years, 2 months ago)

no no no

Just wipe down the package and cut it open and remove the dishes.


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Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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OfflineTeaSippinHippie
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Registered: 11/09/13
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Re: Not another Agar post. [Re: Kenetic]
    #23801230 - 11/04/16 05:09 PM (7 years, 2 months ago)

Ok sweet :awedance:

I just took the PC off the eye, so ill be back in 3 hrs to do my first agar dishes.

Where do i need to place stuff, on the edges? I read countless times to not put your hand over anything. I have my glad bags, syringe, alcohol pads and dishes on the outer edges, ie back and sides


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I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE.
MudaFuka said:
There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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OfflineKenetic
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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23801270 - 11/04/16 05:24 PM (7 years, 2 months ago)

2 hours and 15 minutes at most


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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OfflineTeaSippinHippie
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Re: Not another Agar post. [Re: Kenetic]
    #23801515 - 11/04/16 06:45 PM (7 years, 2 months ago)

Its been almost 2 hours, so I'm going to go agead and move the agar to the SAB and clean everything with the 70% and open up the dishes.


--------------------
I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE.
MudaFuka said:
There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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OfflineTeaSippinHippie
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Registered: 11/09/13
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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23801609 - 11/04/16 07:17 PM (7 years, 2 months ago)

Agar came out of PC with small clump of something, i shook it and it dissolved.

Let the bottle cool in SAB till warm to touch and started pouring, i didn't have enough for all 20 so i must have poured a few to full, there was 5 left over. I put them in glad bags. The others i poured are hopefully solidifying


--------------------
I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE.
MudaFuka said:
There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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Invisibledankington
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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23801633 - 11/04/16 07:29 PM (7 years, 2 months ago)

You put them into bags immediately?
I let my plates sit in the closed up SAB until they set a good hour before putting them in the bags. You may have some sloping in your plates.

Whatever amount of agar works best for you, is the magic number. For me, I've been getting about 20plates out of a snapple bottle (which only really fits 400mL comfortably).


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OfflineTeaSippinHippie
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Re: Not another Agar post. [Re: dankington]
    #23801636 - 11/04/16 07:32 PM (7 years, 2 months ago)

I put the 5 unused ones in bags, and the surface was slightly tilted but not much, after the 5th one i started correcting for the tilt.


--------------------
I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE.
MudaFuka said:
There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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OfflineAlCapone2k
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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23801685 - 11/04/16 07:55 PM (7 years, 2 months ago)

I am a Fan of a bit more agar in the plate. Of course you don't need much agar, but in my opinion more agar in one plate is easier to transfer later, because it's easier to "stab" into the agar wedge with the scalpel.

When I use very thin agar plates, I have the problem that I'm not able to "grab" the wedges for transfer and stabbing is also not that easy. So better use a bit more IMO.

Since agar and LME are the cheapest things in this hobby, you can afford it :P


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Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


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OfflineTeaSippinHippie
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Re: Not another Agar post. [Re: AlCapone2k]
    #23801792 - 11/04/16 08:37 PM (7 years, 2 months ago)

How do i store these? The poured, un poured and the inoculated dishes?


--------------------
I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE.
MudaFuka said:
There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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InvisibleMycolorado
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Registered: 07/23/16
Posts: 8,529
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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23801912 - 11/04/16 09:36 PM (7 years, 2 months ago)

Stacked in the sab.


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🅂🄸🄶🄼🄰


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Offlinenumnum59
Pro-Am Mycologist


Registered: 12/07/11
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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23802696 - 11/05/16 08:11 AM (7 years, 2 months ago)

Quote:

TeaSippinHippie said:
I'm sure ill think the same way after im done.

Do i need to wipe down the petris at all even though they come pre sterilized?



Not unless you want to introduce contams


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OfflineTeaSippinHippie
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Re: Not another Agar post. [Re: numnum59]
    #23802910 - 11/05/16 10:14 AM (7 years, 2 months ago)

Yeah i didn't do it dumb question, im filled with them.


--------------------
I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE.
MudaFuka said:
There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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OfflineSpore Ninja
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Registered: 10/25/16
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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23803072 - 11/05/16 11:25 AM (7 years, 2 months ago)

Did you noc any of them up? After I poured, I hit 2 dishes with 2 drops each of a spore syringe solution. And I have the other plates ready to go when I need to make transfers in a couple of days. You want to hit the myc as soon as it starts to show- before a bunch of strain have formed and start fighting it out.


--------------------
I'm interested in Dr. Pollock's work and his (especially older) strains.
Anyone have pix of his Shroommobile RV??

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OfflineTeaSippinHippie
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Re: Not another Agar post. [Re: Spore Ninja]
    #23803211 - 11/05/16 12:17 PM (7 years, 2 months ago)

Yes i did 4 dishes, all were different amounts of agar so i can test the differences, and i also figured id contam 1 or 2. I also did 2 drops.

That leaves me with 11 poured plates.
I'm ordering 20 more Monday.

I'm happy that it gelled up, i figured I'd mess up so bad it wouldn't.


--------------------
I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE.
MudaFuka said:
There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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OfflineSpore Ninja
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Registered: 10/25/16
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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23806666 - 11/06/16 01:41 PM (7 years, 2 months ago)

Interesting tidbit from workman in a different agar thread:
Quote:

when you take foil wrapped glass dishes out of your PC the foil is going to be wet and I find it nicer to have everything including the package the dishes in dry so wrapping them in foil and baking them is preferred.




--------------------
I'm interested in Dr. Pollock's work and his (especially older) strains.
Anyone have pix of his Shroommobile RV??

PSA: Protonmail offers free, encrypted email hosted in privacy loving Switzerland.
Red Phone / Signal offer free encrypted phone calls and texting between users.

All of these programs are available free for PC and Android or apple and are available in the respective stores or online by googling the names.
Stay safe out there!

The Electronic Freedom Foundation's review of text and messaging program's security is here>>> https://www.eff.org/node/82654


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OfflineTeaSippinHippie
Male


Registered: 11/09/13
Posts: 621
Loc: small sticks town
Last seen: 3 years, 2 months
Re: Not another Agar post. [Re: Spore Ninja]
    #23808222 - 11/06/16 10:18 PM (7 years, 2 months ago)

I was originally looking into the glass petris and bought some because i liked the oven idea, but after realizing I'll be going through a lot of dishes i put them away and bought plastic ones.

On that note no signs of mycelium or contam thats visible. All dishes cleared of condensation except a few of the extras i poured.


--------------------
I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE.
MudaFuka said:
There's nothing wrong with making mistakes as long as you don't make the same ones twice.


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OfflineAlCapone2k
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Registered: 10/06/16
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Re: Not another Agar post. [Re: TeaSippinHippie]
    #23808775 - 11/07/16 07:24 AM (7 years, 2 months ago)

Sorry, wrong thread :smile:

But anyway thanks to enlightment for his answer :smile:


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


Edited by AlCapone2k (11/07/16 08:30 AM)


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Offlineenlightenment
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Re: Not another Agar post. [Re: AlCapone2k]
    #23808791 - 11/07/16 07:38 AM (7 years, 2 months ago)

:pm:

buy the cheaper ones if they are sold as 'sterile'.


Edited by enlightenment (11/07/16 07:45 AM)


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Offlinenumnum59
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Registered: 12/07/11
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Re: Not another Agar post. [Re: enlightenment]
    #23809602 - 11/07/16 12:41 PM (7 years, 2 months ago)

Look into pastywhytes grocery store tek too


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OfflineTeaSippinHippie
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Registered: 11/09/13
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Re: Not another Agar post. [Re: numnum59]
    #23813891 - 11/08/16 07:57 PM (7 years, 2 months ago)



--------------------
I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE.
MudaFuka said:
There's nothing wrong with making mistakes as long as you don't make the same ones twice.


Extras: Filter Print Post Top
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