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numnum59
Pro-Am Mycologist


Registered: 12/07/11
Posts: 1,189
Last seen: 5 months, 22 days
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Pretty much just followed what that guy did
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TeaSippinHippie



Registered: 11/09/13
Posts: 621
Loc: small sticks town
Last seen: 3 years, 2 months
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Re: Not another Agar post. [Re: numnum59]
#23800751 - 11/04/16 02:02 PM (7 years, 2 months ago) |
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So i can just add MEA and water in the bottle and microwave it? For how long?
-------------------- I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE. MudaFuka said: There's nothing wrong with making mistakes as long as you don't make the same ones twice.
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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nevermind the microwave, you got a PC.
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TeaSippinHippie



Registered: 11/09/13
Posts: 621
Loc: small sticks town
Last seen: 3 years, 2 months
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So skip the microwave, add both to bottle and mix and just PC? Should i prep the SAB while the PC is cooling down? Then bring the bottle straight to SAB? And wipe everything down again?
-------------------- I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE. MudaFuka said: There's nothing wrong with making mistakes as long as you don't make the same ones twice.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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mix the MEA and water, boil it so it dissolves. then put into your bottle PC it then cool it down to pouring plates temperature and put it in your SAB. "preparing" a SAB takes like 30 seconds so don't worry about that
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enlightenment
alchemist


Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 8 months, 22 days
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Re: Not another Agar post. [Re: bodhisatta]
#23800837 - 11/04/16 02:37 PM (7 years, 2 months ago) |
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I let the agar jars sit inside the PC. They have a nice pouring temperature about 2 to 3 hours after I turned the heat off. Open the PC after 2 hours and check if you can touch the agar container without burning yourself. The cooler the agar mix, the less condensation will be present after pouring.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Quote:
Bodhisatta said: put them in 47ºC water bath. your agar jars are hotter than 47 so the water bath will warm up. once it cools back down to 47ºC then you're ready to pour.

ready to use
if you pour your dishes correctly at the low temperature you'll end up with something like this
 near the top of the stack you might get a few dishes with some condensation, I actually like to pour stacks tall, so I do them 10 high, if you do a bunch of short stacks you get a bunch of dishes with a little bit of condensation if you do a stack of 10 you get 7-8 perfect dishes and 2 you may have to fix.
how do you fix them?
use a cup with warm water and a flat bottom, put it on top of the dish for a few seconds-minutes (wont hurt if the water is warm-hot) and boom your dishes are crystal clear again.
Why 47?
Quote:
Agar exhibits hysteresis, melting at 85 °C (358 K, 185 °F) and solidifying from 32–40 °C (305–313 K, 90–104 °F). This property lends a suitable balance between easy melting and good gel stability at relatively high temperatures.
I find it starts to gel back up at about 107F or 42C most of the time at the gel strength used for plates. at 47C you can pour a whole sleeve of plates before it gels up on you. and pouring at as low of a temperature as possible minimizes condensation a lot.
to get agar to melt again you have to go all the way back up to 85C, once it is gel simply heating it 47+ won't melt it.
Using more or less A-A in your recipe will make these temperatures and the stiffness of the agar plates a little bit different. Everything here assumes 20g/L or 8g/400mL(20 plates worth)
https://www.shroomery.org/forums/showflat.php/Number/21922023
https://www.shroomery.org/forums/showflat.php/Number/22721954/vc/1#22721954
may as well do it the right way if you're going to do it
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TeaSippinHippie



Registered: 11/09/13
Posts: 621
Loc: small sticks town
Last seen: 3 years, 2 months
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Re: Not another Agar post. [Re: bodhisatta]
#23800878 - 11/04/16 02:54 PM (7 years, 2 months ago) |
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I have that post of yours open for the lady few days lol very informative.
What if i don't have a pot small enough to boil 500ml?
-------------------- I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE. MudaFuka said: There's nothing wrong with making mistakes as long as you don't make the same ones twice.
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numnum59
Pro-Am Mycologist


Registered: 12/07/11
Posts: 1,189
Last seen: 5 months, 22 days
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Put it all in the bottle and heat it up and shake it.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Not another Agar post. [Re: numnum59]
#23800999 - 11/04/16 03:47 PM (7 years, 2 months ago) |
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I think you mean what if I don't have a pot big enough. you could boil 500mL in a soup stock pot if you wanted. just put it in a cup and microwave it to boiling
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TeaSippinHippie



Registered: 11/09/13
Posts: 621
Loc: small sticks town
Last seen: 3 years, 2 months
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Re: Not another Agar post. [Re: numnum59]
#23801005 - 11/04/16 03:50 PM (7 years, 2 months ago) |
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Alright i did, heated it in microwave for 50 sec shook and sat in PC.
-------------------- I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE. MudaFuka said: There's nothing wrong with making mistakes as long as you don't make the same ones twice.
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numnum59
Pro-Am Mycologist


Registered: 12/07/11
Posts: 1,189
Last seen: 5 months, 22 days
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Quote:
TeaSippinHippie said: Alright i did, heated it in microwave for 50 sec shook and sat in PC.
Keep in mind you can swirl it when you pull it out of the pc to mix it up. Just dont overthink it bro. Im out... Good vibes to me ;]
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TeaSippinHippie



Registered: 11/09/13
Posts: 621
Loc: small sticks town
Last seen: 3 years, 2 months
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Re: Not another Agar post. [Re: numnum59]
#23801027 - 11/04/16 03:59 PM (7 years, 2 months ago) |
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Thank you man,
-------------------- I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE. MudaFuka said: There's nothing wrong with making mistakes as long as you don't make the same ones twice.
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TeaSippinHippie



Registered: 11/09/13
Posts: 621
Loc: small sticks town
Last seen: 3 years, 2 months
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Re: Not another Agar post. [Re: bodhisatta]
#23801057 - 11/04/16 04:09 PM (7 years, 2 months ago) |
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With it being so shallow?
-------------------- I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE. MudaFuka said: There's nothing wrong with making mistakes as long as you don't make the same ones twice.
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Spore Ninja
PsychoMycoPhile



Registered: 10/25/16
Posts: 161
Last seen: 5 years, 6 months
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Quote:
TeaSippinHippie said: Alright i did, heated it in microwave for 50 sec shook and sat in PC.
As long as you added it to hot water and stirred it or swirled it you should be fine.
I had 500ML in a pyrex measuring cup, put it in the microwave for 2 minutes and then stirred in my agar.
It's going to boil in the PC, so that should takecare of the rest of the mixing.
-------------------- I'm interested in Dr. Pollock's work and his (especially older) strains. Anyone have pix of his Shroommobile RV?? PSA: Protonmail offers free, encrypted email hosted in privacy loving Switzerland. Red Phone / Signal offer free encrypted phone calls and texting between users. All of these programs are available free for PC and Android or apple and are available in the respective stores or online by googling the names. Stay safe out there! The Electronic Freedom Foundation's review of text and messaging program's security is here>>> https://www.eff.org/node/82654
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TeaSippinHippie



Registered: 11/09/13
Posts: 621
Loc: small sticks town
Last seen: 3 years, 2 months
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Using PC freaks me out. This thing sounds mad.
-------------------- I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE. MudaFuka said: There's nothing wrong with making mistakes as long as you don't make the same ones twice.
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numnum59
Pro-Am Mycologist


Registered: 12/07/11
Posts: 1,189
Last seen: 5 months, 22 days
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Quote:
TeaSippinHippie said: Using PC freaks me out. This thing sounds mad.
Is this your first time? Adjust the heat until steam is just escaping or the weight is just jiggling
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TeaSippinHippie



Registered: 11/09/13
Posts: 621
Loc: small sticks town
Last seen: 3 years, 2 months
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Re: Not another Agar post. [Re: numnum59]
#23801207 - 11/04/16 04:58 PM (7 years, 2 months ago) |
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About my 6th time using one. But my grandmother had one blow up on her.
-------------------- I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE. MudaFuka said: There's nothing wrong with making mistakes as long as you don't make the same ones twice.
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
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Re: Not another Agar post. [Re: numnum59]
#23801211 - 11/04/16 05:00 PM (7 years, 2 months ago) |
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You are definitely over-thinking this. Agar is easy as fuck
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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TeaSippinHippie



Registered: 11/09/13
Posts: 621
Loc: small sticks town
Last seen: 3 years, 2 months
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Re: Not another Agar post. [Re: Kenetic]
#23801218 - 11/04/16 05:03 PM (7 years, 2 months ago) |
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I'm sure ill think the same way after im done.
Do i need to wipe down the petris at all even though they come pre sterilized?
-------------------- I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE. MudaFuka said: There's nothing wrong with making mistakes as long as you don't make the same ones twice.
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