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TeaSippinHippie



Registered: 11/09/13
Posts: 621
Loc: small sticks town
Last seen: 3 years, 2 months
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Re: Not another Agar post. [Re: numnum59]
#23795190 - 11/02/16 05:01 PM (7 years, 2 months ago) |
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I think that bod quote is from a page about agar i have saved from him haha
Edited by TeaSippinHippie (11/02/16 05:05 PM)
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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I would not pour and wrap, pour the whole stack and then either put all the petris unwrapped in a ziploc bag oder wrap them all after pouring the whole stack with saran wrap/parafilm.
If you pour and wrap every single dish, your agar could cool down and you could get some problems with the last petris, because your agar got sticky.
And yes, the quote is from the "Pour Agar Tek" from bodhi, which is in my opinion the best one to go for.
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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TeaSippinHippie



Registered: 11/09/13
Posts: 621
Loc: small sticks town
Last seen: 3 years, 2 months
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Re: Not another Agar post. [Re: AlCapone2k]
#23795625 - 11/02/16 07:22 PM (7 years, 2 months ago) |
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Am i wraping the whole dish lid and all? Wraping afterwards sounds better so I'll definitely do it that way.
-------------------- I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE. MudaFuka said: There's nothing wrong with making mistakes as long as you don't make the same ones twice.
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Greg
always learning




Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 3 months, 3 days
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Re: Not another Agar post. [Re: AlCapone2k]
#23795628 - 11/02/16 07:24 PM (7 years, 2 months ago) |
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No need to wrap after pouring. Only after inoculating as many others have said.
My unused plates sit in stacks in the SAB until I inoculate them. My unpoured plates sit in stacks just like the poured ones, sometimes I keep the original sleeve on them too. (This doesn't really apply to glass petris though as they don't come in sleeves presterile like my plastic ones do.)
They sit there sometimes upwards of a week without issue. I've only ever had 3 contam before using them out of 300+ I've poured.
I have two stacks of poured plates, one is regular ME/PD agar and the other is antibacterial agar (dyed red). I germinate spores and take clones on the AB agar if I have it handy, otherwise I just use regular MEA/PDA. Everything else is always done on MEA or PDA.

Follow this link to see how I use Parafilm to wrap dishes after inoculating: https://www.shroomery.org/forums/showflat.php/Number/23717103 You just wrap the edges but it's OK if a little bit overlaps on to the top or bottom of the plate.
Edited by Greg (11/02/16 07:29 PM)
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TeaSippinHippie



Registered: 11/09/13
Posts: 621
Loc: small sticks town
Last seen: 3 years, 2 months
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Re: Not another Agar post. [Re: Greg]
#23795653 - 11/02/16 07:35 PM (7 years, 2 months ago) |
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I bought a 20pk of plastic pre sterilized. Good information thank you
-------------------- I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE. MudaFuka said: There's nothing wrong with making mistakes as long as you don't make the same ones twice.
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Spore Ninja
PsychoMycoPhile


Registered: 10/25/16
Posts: 161
Last seen: 5 years, 6 months
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Quote:
TeaSippinHippie said: Am i wraping the whole dish lid and all? Wraping afterwards sounds better so I'll definitely do it that way.
Yes, wrap it only after it's inoculated. With the the plastic petris, you can safely store them back in the original plastic sleeve after you pour them.
If you wrap the glass ones in foil when you PC them they should be OK that way, especially if you store them in a clean SAB. Put them in a gallon size ziplock for an extra level of security if you feel the need.
-------------------- I'm interested in Dr. Pollock's work and his (especially older) strains. Anyone have pix of his Shroommobile RV?? PSA: Protonmail offers free, encrypted email hosted in privacy loving Switzerland. Red Phone / Signal offer free encrypted phone calls and texting between users. All of these programs are available free for PC and Android or apple and are available in the respective stores or online by googling the names. Stay safe out there! The Electronic Freedom Foundation's review of text and messaging program's security is here>>> https://www.eff.org/node/82654
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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70% alcohol is better at sanitizing than 90+ I saw 99% alcohol on the other page.
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TeaSippinHippie



Registered: 11/09/13
Posts: 621
Loc: small sticks town
Last seen: 3 years, 2 months
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Re: Not another Agar post. [Re: bodhisatta]
#23796821 - 11/03/16 07:04 AM (7 years, 2 months ago) |
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So i need to get 70 instead?
-------------------- I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE. MudaFuka said: There's nothing wrong with making mistakes as long as you don't make the same ones twice.
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numnum59
Pro-Am Mycologist


Registered: 12/07/11
Posts: 1,189
Last seen: 5 months, 22 days
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70% percent of alcohol is ideal to a stronger solution. Pure alcohol coagulates protein in contact. Suppose the pure alcohol is poured over a single celled organism. The alcohol will go through the cell wall of the organism in all direction, coagulating the protein just inside the cell wall. The ring of the coagulated protein would then stop the alcohol from penetrating farther from the cell, and no more coagulation would take place. At this time the cell would become inactive but not dead. Under the favorable conditions the cell would then begin to function. If 70 percent of alcohol is poured to a single celled organism, the diluted alcohol also coagulates the protein, but at a slower rate, so that it penetrates all the way through the cell before coagulation can block it. Then the entire cell is coagulated and the organism dies.
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TeaSippinHippie



Registered: 11/09/13
Posts: 621
Loc: small sticks town
Last seen: 3 years, 2 months
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Re: Not another Agar post. [Re: numnum59]
#23797006 - 11/03/16 09:16 AM (7 years, 2 months ago) |
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Gotta love science
-------------------- I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE. MudaFuka said: There's nothing wrong with making mistakes as long as you don't make the same ones twice.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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You can water it down to 70%
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TeaSippinHippie



Registered: 11/09/13
Posts: 621
Loc: small sticks town
Last seen: 3 years, 2 months
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Re: Not another Agar post. [Re: bodhisatta]
#23797125 - 11/03/16 10:26 AM (7 years, 2 months ago) |
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I'll go out and get the 70% and toss the strong stuff in the emergency farm bag.
-------------------- I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE. MudaFuka said: There's nothing wrong with making mistakes as long as you don't make the same ones twice.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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90+ is good for your bongs
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TeaSippinHippie



Registered: 11/09/13
Posts: 621
Loc: small sticks town
Last seen: 3 years, 2 months
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Re: Not another Agar post. [Re: bodhisatta]
#23797219 - 11/03/16 11:03 AM (7 years, 2 months ago) |
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Haha yeah, we have one the bong too.
-------------------- I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE. MudaFuka said: There's nothing wrong with making mistakes as long as you don't make the same ones twice.
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TeaSippinHippie



Registered: 11/09/13
Posts: 621
Loc: small sticks town
Last seen: 3 years, 2 months
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Thinking tonights the night. I got everything i need.
-------------------- I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE. MudaFuka said: There's nothing wrong with making mistakes as long as you don't make the same ones twice.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Spore Ninja
PsychoMycoPhile


Registered: 10/25/16
Posts: 161
Last seen: 5 years, 6 months
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Quote:
TeaSippinHippie said: Thinking tonights the night. I got everything i need.
You reminded me I need to do so isolation and agar work...cleaning the kitchen now so I can get set up to pour. Think I'll do dog food agar with some activated charcoal for color.
-------------------- I'm interested in Dr. Pollock's work and his (especially older) strains. Anyone have pix of his Shroommobile RV?? PSA: Protonmail offers free, encrypted email hosted in privacy loving Switzerland. Red Phone / Signal offer free encrypted phone calls and texting between users. All of these programs are available free for PC and Android or apple and are available in the respective stores or online by googling the names. Stay safe out there! The Electronic Freedom Foundation's review of text and messaging program's security is here>>> https://www.eff.org/node/82654
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TeaSippinHippie



Registered: 11/09/13
Posts: 621
Loc: small sticks town
Last seen: 3 years, 2 months
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I have ziplock bags to store dishes until i put spores to them, but afterwards can i wrap in gladwrap and put back in ziplock? Making sure first.
-------------------- I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE. MudaFuka said: There's nothing wrong with making mistakes as long as you don't make the same ones twice.
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numnum59
Pro-Am Mycologist


Registered: 12/07/11
Posts: 1,189
Last seen: 5 months, 22 days
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Quote:
TeaSippinHippie said: I have ziplock bags to store dishes until i put spores to them, but afterwards can i wrap in gladwrap and put back in ziplock? Making sure first.
I just store them nocd up or not in ziplocks without wrapping them. Just use a new ziplock each time.
Cutting seran wrap is a bitch and a half
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TeaSippinHippie



Registered: 11/09/13
Posts: 621
Loc: small sticks town
Last seen: 3 years, 2 months
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Re: Not another Agar post. [Re: numnum59]
#23800617 - 11/04/16 12:57 PM (7 years, 2 months ago) |
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Ok thank you, also the directions on the MEA say 20gs to 500ml, simmer to fully dissolved then sterilize at 10psi for 30 mins. Can i just set the glass bottle in a pot of water to boil? Or do i need to boil it in a small pot then put it in the bottle to PC?
I might just be over thinking this haha
-------------------- I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE. MudaFuka said: There's nothing wrong with making mistakes as long as you don't make the same ones twice.
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