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Phycus
Blister Lips



Registered: 12/08/13
Posts: 210
Loc: tippy top of the tower
Last seen: 2 months, 17 days
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6 strains. 1 decision.
#23791859 - 11/01/16 04:05 PM (7 years, 2 months ago) |
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hey guys i know there are tons of threads asking for which strain to start with yada yada BUT im going to make this easier. i have 20ccs of costa rica 10ccs of Malabar 30ccs of Mazatapec 10ccs of PES Amazonian 10ccs of Ecuador and 10ccs of Burma. i mainly do straw logs just to get away from having so many ratios to deal with, as opposed to tubs but I would still like to try a tub. Right now, i want to pick from these spores and make clones to isolate a single genetic line to reproduce the same mushroom every grow. i Know that after the second flush i will have smaller yields etc. So... this being said i am not worried about the yield, rather quality. id rather have 1g of something thatll lift my lid as opposed to having to eat a 1/4 or an 8er. ironically i hate the taste of non gourmet mushrooms so i usually make tea. anyways i plan to isolate these as said but then comes my second question : what medium should i use for this process? Petri dishes look like too much work and hazard but on the other hand i don't really trust the honey tek (never tried don't kill me). any input would be great!
-------------------- disclaimer - nothing i post is real. this account is for fictitious purposes and posts should not be taken literally
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: 6 strains. 1 decision. [Re: Phycus]
#23792038 - 11/01/16 04:45 PM (7 years, 2 months ago) |
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Just pick one. Petri dishes are a cultivators best friend
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Greg
always learning




Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 3 months, 3 days
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Re: 6 strains. 1 decision. [Re: bodhisatta]
#23792052 - 11/01/16 04:48 PM (7 years, 2 months ago) |
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If someone as thick in the head and backwards as myself can make over 300 petri dishes with only 3 contams, so can you! Seriously, agar is dead easy.
Click the link in Bod's signature and then click on the agar link for more info. (Or click the one in my sig that says Bodhisatta's agar portal, they're the same link.)
Also, for all intents and purposes all of those 'strains' are essentially the same. Agar can help you select for potency until you have a culture that knocks your socks off.
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Phycus
Blister Lips



Registered: 12/08/13
Posts: 210
Loc: tippy top of the tower
Last seen: 2 months, 17 days
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Re: 6 strains. 1 decision. [Re: Greg]
#23792106 - 11/01/16 05:06 PM (7 years, 2 months ago) |
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I understand the "cube is a cube" mindset most ppl have but that's until they do isolate a strain. Yah I went ahead with the PRs. 1cc per half pint. I wonder if that may be too much given where theyre from and maybe introducing too many genetics at once for such a small container, hopefully this wont cause stalling. my medium is rye berries fyi. as far as agar and cloning go, I know im at least 6wks away from today from a harvest. I know 6 wks is optimal time but that's what im hoping for. my next question is, how do I know which mushie to pick without eating it to test for potency first? bigger isn't better and sometimes the small fries are worthless. which flush should I pick from? I will definitely look at those teks btw.
side note : I am also building an 8x10 positive pressure room for all of this and I cant really find what I need on these boards as far as an example goes. I know its overkill to build one really but I want 0% chance of contams.
-------------------- disclaimer - nothing i post is real. this account is for fictitious purposes and posts should not be taken literally
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: 6 strains. 1 decision. [Re: Phycus]
#23792112 - 11/01/16 05:08 PM (7 years, 2 months ago) |
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You clone mushrooms then eat them. If they're bunk then throw out that plate
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Phycus
Blister Lips



Registered: 12/08/13
Posts: 210
Loc: tippy top of the tower
Last seen: 2 months, 17 days
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Re: 6 strains. 1 decision. [Re: bodhisatta]
#23792143 - 11/01/16 05:17 PM (7 years, 2 months ago) |
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good lord im gonna have a pile of mutilated mushies then! I guess that's the only way to tell without a mass spectrometer.
-------------------- disclaimer - nothing i post is real. this account is for fictitious purposes and posts should not be taken literally
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Phycus
Blister Lips



Registered: 12/08/13
Posts: 210
Loc: tippy top of the tower
Last seen: 2 months, 17 days
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Re: 6 strains. 1 decision. [Re: Phycus]
#23797801 - 11/03/16 02:05 PM (7 years, 2 months ago) |
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update: nocked up 6 1/2 pint jars with EC 2 days ago and nothing... not sure why or how. theyre kept in a drawer in a room with no moving air at 73 degrees on rye. ive never had this happen. not one spot or spec and ive been over them with a magnifying glass to make sure. the lids have SHIPs and 4 1/16in holes double covered with micro tape. made more jars a few mins ago and nocked em up with PR, same exact way. the grain im using is a few yrs old but they got to field cap moisture no problem at all. same smell when pc'd, same look. any ideas? im thinking there may be some serious competition going on in the jars since I did put in 1cc/jar and im used to putting that much in a pint sized jar. idk. . I highly recommend buying on of their stocking deals btw around Christmas time. even came with a badass candy cane
-------------------- disclaimer - nothing i post is real. this account is for fictitious purposes and posts should not be taken literally
Edited by cronicr (11/03/16 03:06 PM)
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: 6 strains. 1 decision. [Re: Phycus]
#23797863 - 11/03/16 02:24 PM (7 years, 2 months ago) |
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its only 2 days. it can take 2 weeks to see growth from MS on pf.
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Phycus
Blister Lips



Registered: 12/08/13
Posts: 210
Loc: tippy top of the tower
Last seen: 2 months, 17 days
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Re: 6 strains. 1 decision. [Re: mushboy]
#23797897 - 11/03/16 02:37 PM (7 years, 2 months ago) |
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im not doing pf tek. this is my 5th grow and usually theres at least a small start to the myc by now on the glass. I can see spores on the berries themselves. but ive been looking over the boards for issues that ppl have with their ms projects. but yeah in the end youre right. be patient don't be a patient.
-------------------- disclaimer - nothing i post is real. this account is for fictitious purposes and posts should not be taken literally
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freespeech
disciple



Registered: 12/12/08
Posts: 1,745
Loc: PNW
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Re: 6 strains. 1 decision. [Re: Phycus]
#23797907 - 11/03/16 02:40 PM (7 years, 2 months ago) |
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Don't talk about growing from a named vendor's spores in the cultivation forum. I guess you didn't read the rules thread before posting 
https://www.shroomery.org/forums/showflat.php/Number/14960167
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: 6 strains. 1 decision. [Re: freespeech]
#23797977 - 11/03/16 03:06 PM (7 years, 2 months ago) |
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I edited it out
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Phycus
Blister Lips



Registered: 12/08/13
Posts: 210
Loc: tippy top of the tower
Last seen: 2 months, 17 days
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Re: 6 strains. 1 decision. *DELETED* [Re: cronicr]
#23798013 - 11/03/16 03:13 PM (7 years, 2 months ago) |
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Post deleted by PhycusReason for deletion: off topic
-------------------- disclaimer - nothing i post is real. this account is for fictitious purposes and posts should not be taken literally
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: 6 strains. 1 decision. [Re: Phycus]
#23798017 - 11/03/16 03:15 PM (7 years, 2 months ago) |
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He isn't trolling.
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Phycus
Blister Lips



Registered: 12/08/13
Posts: 210
Loc: tippy top of the tower
Last seen: 2 months, 17 days
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Re: 6 strains. 1 decision. [Re: cronicr]
#23801456 - 11/04/16 06:23 PM (7 years, 2 months ago) |
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update: EC jars have no sign of myc formation. not one of the six. So Wednesday I made 5 more 1/2 pint jars with PR ms aaaaaaand they've started colonizing. idk how to explain it. some threads lead me to believe that I may have just gotten a bunk syringe (unlikely as the vendor is widely known to be top notch) or I used too much. the second batch only has 1/2 cc/jar. I may keep the ECs just to see if theyre gonna take some time as Mushboy said. idk. anyways ive decided to switch from straw logs to monotubs. ive looked over franks tek, spitballs tek and a few others. im going to try to experiment a little, unless anyone has any experience in what im about to get going. I have a couple of 66qt tubs that are blue plastic with snaplock lids. im thinking about putting a layer of clear plastic over the top, after I spawn to whatever substrate I choose, with double sided tape to keep the top sealed tight. im doing this so I can use light during fruiting (couple of 5k lights daisy chained) so I can have light directly above the tubs so the mushies come up nice and straight as well as benefit from the light. any links or personal advice is always welcome!
-------------------- disclaimer - nothing i post is real. this account is for fictitious purposes and posts should not be taken literally
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