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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
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Clean room 1
#23790954 - 11/01/16 10:49 AM (7 years, 2 months ago) |
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So to the home cultivator what all do you keep in your inoculation room
if it isnt your bathroom or something like that for ten minutes
Edited by spore-ty (11/01/16 10:52 AM)
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Terpfreak
❀Terpenes❀



Registered: 07/08/16
Posts: 1,065
Loc: Land Of Ooo
Last seen: 5 years, 10 months
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: following cos for me it's my bathroom or something for 30 mins.
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thebug76
2 years in.



Registered: 05/31/15
Posts: 557
Loc: North of the equater and ...
Last seen: 3 months, 26 days
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Normal dining room junk. I use my dining room table. And yea, it's more like 30 mins.
-------------------- Bug
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Quote:
spore-ty said: So to the home cultivator what all do you keep in your inoculation room
if it isnt your bathroom or something like that for ten minutes
having a hard time figuring out wtf you are asking. but my SAB is in my basement. no air vents in basement. only a cat box. a sofa. a tv. and some random shit.
fyi. bathrooms are not good for inoculation. lots of bacteria floating around. even a clean toilet is pretty funky
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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
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Re: Clean room [Re: mushboy]
#23791113 - 11/01/16 11:49 AM (7 years, 2 months ago) |
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Im asking more for the people who have a room for inoculation with a flowhood, stainless steel table and etc. Do you guys keep your clean spawn in there , do you keep substrate/spawn materials in there etc.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Clean room [Re: spore-ty] 1
#23791129 - 11/01/16 11:55 AM (7 years, 2 months ago) |
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People keep them where there is room
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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
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Well do you keep your spawn with your flow hood and all that
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Clean room [Re: spore-ty] 1
#23791240 - 11/01/16 12:44 PM (7 years, 2 months ago) |
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No flowhood its pretty damn overkill unless you make a hundred quarts of spawn a week. A plastic box does just fine. My jars go on a empty bookshelf
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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
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I do intend on inoculating hundreds perhaps even thousands a week eventually with my farm but thats a whole nother post, im just tryna see what people keep with their flow hood stuff and all
Also do you use a sab still?
Edited by spore-ty (11/01/16 12:55 PM)
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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
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Better explanation heres what I keep in here still working on it gonna add shelving to the walls to let jars colonize, paper towel holder, flowhood etc. But here it.is so far
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Quote:
spore-ty said: I do intend on inoculating hundreds perhaps even thousands a week eventually with my farm but thats a whole nother post, im just tryna see what people keep with their flow hood stuff and all
Also do you use a sab still?
One of those people huh.
-------------------- KSSS And PE WBS.
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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
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Quote:
Moabfighter said:
Quote:
spore-ty said: I do intend on inoculating hundreds perhaps even thousands a week eventually with my farm but thats a whole nother post, im just tryna see what people keep with their flow hood stuff and all
Also do you use a sab still?
One of those people huh.
Lol FOH nooblet
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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RR did it in his bedroom lol
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Apes



Registered: 06/24/10
Posts: 115
Last seen: 11 months, 15 days
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Re: Clean room [Re: mushboy]
#23798572 - 11/03/16 06:12 PM (7 years, 2 months ago) |
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Quote:
spore-ty said:
fyi. bathrooms are not good for inoculation. lots of bacteria floating around. even a clean toilet is pretty funky
Also why you should never use the hand blow-dryer in a public restroom. They're not pumping that air in from outside..
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 14 hours, 25 minutes
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Quote:
spore-ty said:

How did you hack those holes?
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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99.99
Stranger


Registered: 12/22/14
Posts: 1,933
Last seen: 2 hours, 29 minutes
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Quote:
bodhisatta said: No flowhood its pretty damn overkill unless you make a hundred quarts of spawn a week. A plastic box does just fine. My jars go on a empty bookshelf
Overkill whats that i use a 4X4 FOOT laminar hood lol... I turn the top on a hour before i do whats needed and bottom on half hour before
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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
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Quote:
wtfcrazymofo said:
Quote:
spore-ty said:

How did you hack those holes?
With my teeth durrr
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 14 hours, 25 minutes
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Re: Clean room [Re: 99.99]
#23799731 - 11/04/16 04:26 AM (7 years, 2 months ago) |
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Quote:
99.99 said:
Quote:
bodhisatta said: No flowhood its pretty damn overkill unless you make a hundred quarts of spawn a week. A plastic box does just fine. My jars go on a empty bookshelf
Overkill whats that i use a 4X4 FOOT laminar hood lol... I turn the top on a hour before i do whats needed and bottom on half hour before

nice flow wall
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Clean room [Re: 99.99]
#23799803 - 11/04/16 06:12 AM (7 years, 2 months ago) |
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Quote:
99.99 said: Overkill whats that i use a 4X4 FOOT laminar hood lol... I turn the top on a hour before i do whats needed and bottom on half hour before

I think I would get really bad cotton mouth in front of that
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Clear your sinuses right up
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Clean room [Re: azur]
#23799836 - 11/04/16 06:41 AM (7 years, 2 months ago) |
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need a paper weight for those parafilm strips too
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Mushierage
SWIM Sinker



Registered: 06/29/16
Posts: 1,094
Last seen: 7 years, 1 month
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I don't feel any need for a clean room. I barely have any contamination as is, and when I do, I usually am expecting it because of something I know I did incorrectly, or not with care.
I just spray a bit of Lysol around my room and inside my sab, let the air settle for about an hour, and go into that room with no moving air, and do my business. When I'm doing clean work (inoculating, agar, transfers, etc) generally I'll try to do all those things all at once, so I'll consolidate my work in one small area of the room and make sure everything is quickly accessible to me, that way I'm not getting up constantly and kicking around air with potential contaminants in it.
I have a 95% success rate nearly with my methods. If I were to clamp down on every clean step I could, and be really anal about it, I could get that up to 99% pretty easily.
It doesn't take a mad scientist lab and a degree in mycology to be really successful at this. All you really need to do is practice the sterile tek steps that are most important, like using an SAB, letting air settle, flaming tools, using gloves... just use common sense.
In every cubic inch of air you breath, there are thousands of contaminants floating around. The only way to get away from them it to literally let them settle out of the air, and inoculate when they aren't floating around. (Aka inside your SAB after the air has settled. It's common sense).
-------------------- Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup. OR... You should be cultivating reptiles and fish, not mushrooms.
  
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Is it truly 95%? Of 89%? I'm genuinely curious. And what do you base that on to get a percentage? A lot of guys on here say the same thing and it never made sense. How does one figure the success rate? Is it with tubs only? Or jars and plates too?
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morty422
Wuba-luba-dub-dub!


Registered: 07/06/16
Posts: 988
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Quote:
Mushierage said: I don't feel any need for a clean room. I barely have any contamination as is, and when I do, I usually am expecting it because of something I know I did incorrectly, or not with care.
I just spray a bit of Lysol around my room and inside my sab, let the air settle for about an hour, and go into that room with no moving air, and do my business. When I'm doing clean work (inoculating, agar, transfers, etc) generally I'll try to do all those things all at once, so I'll consolidate my work in one small area of the room and make sure everything is quickly accessible to me, that way I'm not getting up constantly and kicking around air with potential contaminants in it.
I have a 95% success rate nearly with my methods. If I were to clamp down on every clean step I could, and be really anal about it, I could get that up to 99% pretty easily.
It doesn't take a mad scientist lab and a degree in mycology to be really successful at this. All you really need to do is practice the sterile tek steps that are most important, like using an SAB, letting air settle, flaming tools, using gloves... just use common sense.
In every cubic inch of air you breath, there are thousands of contaminants floating around. The only way to get away from them it to literally let them settle out of the air, and inoculate when they aren't floating around. (Aka inside your SAB after the air has settled. It's common sense).

I haven't gotten a contamination in a jar or in a clean agar transfer in a long while... I do exactly what mushierage does. 
The only time I get a contaminate is when I know I will.
"oops...dropped that wedge on the ground... eh... 5 second rule..."
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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I lost a 40 jar run of masters recently. Was in a hurry to get out and cut my cook time short. Contamination is part of this game. You'll never not have any. Just doesn't work that way. Mushierage, I'm a lysol fan too.
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Clean room [Re: azur]
#23800066 - 11/04/16 08:42 AM (7 years, 2 months ago) |
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oh man, the shroomery's entertaining  spraying a surface disinfectant in the air, talking about common sense in the same post!
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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I didn't say if I spray it in the air or not. Although, one would think it would certainly help particles in the air fall. Don't hate cause you can't afford lysol
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Clean room [Re: azur]
#23800087 - 11/04/16 08:50 AM (7 years, 2 months ago) |
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if you didnt say it then perhaps I wasn't talking about you then? :plotthickens:
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Your reply was to me though.... I do use it to wipe down the bottom of my fh. And I use lysol wipes to clean my masters before g2ging. And I did use the lysol air shit when I had a still air box. And if I had to go back to a still air box, I would use it again
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Clean room [Re: azur]
#23800101 - 11/04/16 08:56 AM (7 years, 2 months ago) |
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ah, reply fail confusion. how about some "common sense"? :P
but yeah surface disinfectants are great for disinfecting surfaces as you say. But spraying the room you set up your SAB in with lysol is a waste of lysol.
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Common sense? What is that?
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Clean room [Re: azur] 1
#23800128 - 11/04/16 09:08 AM (7 years, 2 months ago) |
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Common sense aint common enough anymore (true quote).
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Spore Ninja
PsychoMycoPhile


Registered: 10/25/16
Posts: 161
Last seen: 5 years, 6 months
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Quote:
spore-ty said: Better explanation heres what I keep in here still working on it gonna add shelving to the walls to let jars colonize, paper towel holder, flowhood etc. But here it.is so far
[url=https://files.shroomery.org/files/16-44/819966187-20161103_145025.jpg][image]/forums/thumbs/16-44/819966187-
In a galaxy far far away...
I pulled the carpet out and laid down new, heavy duty linoleum flooring. Put heavy duty commercial rubber baseboards in. Painted the walls and ceiling with 2 coats of easy to wash flat white.
I had one clear tub for my clean surgical scrubs. Another upright cabinet for larger supplies, and a couple of rolling carts with sliding drawers for smaller supplies like gloves, masks, syringes etc..
I had a table with the flow hood, and a separate 4ftX4ft space framed out in PVC pipe and covered in 6mil clear plastic sheeting as sort of an 'isolation' area. SAB, poured plates works in progress stayed in there, separate from the work/grow area. The idea being, if a tub contamed, it hopefully wouldn't be able to spread to the isolated mini clean room.
Big round Honeywell HEPA's on the floor ran 24/7 unless the flowhood was on. Incoming AC vents were filtered. A small dorm fridge in the room held samples.
OCD is a lifestyle, not a hobby.
-------------------- I'm interested in Dr. Pollock's work and his (especially older) strains. Anyone have pix of his Shroommobile RV?? PSA: Protonmail offers free, encrypted email hosted in privacy loving Switzerland. Red Phone / Signal offer free encrypted phone calls and texting between users. All of these programs are available free for PC and Android or apple and are available in the respective stores or online by googling the names. Stay safe out there! The Electronic Freedom Foundation's review of text and messaging program's security is here>>> https://www.eff.org/node/82654
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Spore Ninja
PsychoMycoPhile


Registered: 10/25/16
Posts: 161
Last seen: 5 years, 6 months
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Quote:
spore-ty said: Better explanation heres what I keep in here still working on it gonna add shelving to the walls to let jars colonize, paper towel holder, flowhood etc. But here it.is so far

Awesome arm holes in that SAB...
-------------------- I'm interested in Dr. Pollock's work and his (especially older) strains. Anyone have pix of his Shroommobile RV?? PSA: Protonmail offers free, encrypted email hosted in privacy loving Switzerland. Red Phone / Signal offer free encrypted phone calls and texting between users. All of these programs are available free for PC and Android or apple and are available in the respective stores or online by googling the names. Stay safe out there! The Electronic Freedom Foundation's review of text and messaging program's security is here>>> https://www.eff.org/node/82654
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Dedication!
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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And some arm chafing. Those are the keys to epic cultivation
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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hehe, teaches you to keep you arms the fuck away from the SAB itself
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Marty Mycfly
Time Traveler


Registered: 12/16/13
Posts: 976
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Quote:
spore-ty said: Im asking more for the people who have a room for inoculation with a flowhood, stainless steel table and etc. Do you guys keep your clean spawn in there , do you keep substrate/spawn materials in there etc.
It's always best have a separate incubation area for spawn and sub, however I don't and does cause some issues, a room packed with sub get's super warm and running the hood makes it even warmer, and then it gets super hot when you are cooling sub or spawn in there. I suggest if your designing a spot, keep in mind an incubation area as well that is not in your lab. Here is a kind of 360 view of my lab/incubation area
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 14 hours, 25 minutes
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Quote:
Marty Mycfly said:
Quote:
spore-ty said: Im asking more for the people who have a room for inoculation with a flowhood, stainless steel table and etc. Do you guys keep your clean spawn in there , do you keep substrate/spawn materials in there etc.
It's always best have a separate incubation area for spawn and sub, however I don't and does cause some issues, a room packed with sub get's super warm and running the hood makes it even warmer, and then it gets super hot when you are cooling sub or spawn in there. I suggest if your designing a spot, keep in mind an incubation area as well that is not in your lab. Here is a kind of 360 view of my lab/incubation area

-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
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That's fucking awesome!
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
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Quote:
spacechildo said: Common sense aint common enough anymore (true quote).
True that Quote:
Marty Mycfly said:
Quote:
spore-ty said: Im asking more for the people who have a room for inoculation with a flowhood, stainless steel table and etc. Do you guys keep your clean spawn in there , do you keep substrate/spawn materials in there etc.
It's always best have a separate incubation area for spawn and sub, however I don't and does cause some issues, a room packed with sub get's super warm and running the hood makes it even warmer, and then it gets super hot when you are cooling sub or spawn in there. I suggest if your designing a spot, keep in mind an incubation area as well that is not in your lab. Here is a kind of 360 view of my lab/incubation area

Fucking thank you answered my questions on the head and stuff I didnt think of now currently am and alot more of other little things thanks a ton oh ya very noiice set up
And azur i know you got enough sense to post some lab pics mane
Edited by spore-ty (11/04/16 10:10 PM)
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Yea. I told someone a week ago I would. Let me see if I have any in my gallery... I store spawn here
 Here's the main lab area. Shower behind the camera. Flow hood on left. Tables and sinks to right. Burners and pc's straight ahead.
 And in the back is a small completely automated room. With fresh hepa filtered air coming from outside and a humidifier with a direct water feed.
 These pics are a little old and the place was a mess. I try to keep it tidier than that.
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99.99
Stranger


Registered: 12/22/14
Posts: 1,933
Last seen: 2 hours, 29 minutes
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Re: Clean room [Re: azur]
#23802096 - 11/04/16 10:47 PM (7 years, 2 months ago) |
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Azur you're going to die from carbon monoxide poisoning from your huge pressure cooker ... Lol
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Re: Clean room [Re: 99.99]
#23802104 - 11/04/16 10:50 PM (7 years, 2 months ago) |
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No I'm not. I run it to the right where it is in the picture. There is a hood with a big exhaust fan there.
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Marty Mycfly
Time Traveler


Registered: 12/16/13
Posts: 976
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Re: Clean room [Re: azur]
#23802106 - 11/04/16 10:51 PM (7 years, 2 months ago) |
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Quote:
azur said: Yea. I told someone a week ago I would. Let me see if I have any in my gallery... I store spawn here
 Here's the main lab area. Shower behind the camera. Flow hood on left. Tables and sinks to right. Burners and pc's straight ahead.
 And in the back is a small completely automated room. With fresh hepa filtered air coming from outside and a humidifier with a direct water feed.
 These pics are a little old and the place was a mess. I try to keep it tidier than that.
Nice setup You can get some shit done up in there! Sexy ass SS. And gnarly p.c! I think I remember seeing a thread about it a long time ago? Id like to know more about and how it runs, I don't wanna thread jack though.
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Pc is gas and forced air fired. Uses a 941 lid. Holds 21-27 bags (monos) in one run. I only use it when I'm running behind or to sterilize wood substrates. Takes a lot of grain to fill her up.
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 14 hours, 25 minutes
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Quote:
spore-ty said: Better explanation heres what I keep in here still working on it gonna add shelving to the walls to let jars colonize, paper towel holder, flowhood etc. But here it.is so far

Quote:
spore-ty said:
Quote:
wtfcrazymofo said:
Quote:
spore-ty said:

How did you hack those holes?
With my teeth durrr
That's the way I hack um too.
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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Mushierage
SWIM Sinker



Registered: 06/29/16
Posts: 1,094
Last seen: 7 years, 1 month
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Re: Clean room [Re: azur]
#23802432 - 11/05/16 03:40 AM (7 years, 2 months ago) |
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Quote:
azur said: I didn't say if I spray it in the air or not. Although, one would think it would certainly help particles in the air fall. Don't hate cause you can't afford lysol
Quote:
azur said: Is it truly 95%? Of 89%? I'm genuinely curious. And what do you base that on to get a percentage? A lot of guys on here say the same thing and it never made sense. How does one figure the success rate? Is it with tubs only? Or jars and plates too?
It's just a measurement of success vs. failure in regards to how many grows I've done in this last run that have bore fruit without being tossed because of some damning contamination like trich. For me, it's just for tubs, but for somebody else doing multiple grows, you could probably use an equation to figure it out by the volume of spawn you use in any grow, or perhaps the amount of sub altogether.
It isn't perfect but it gives an idea of how well or shitty I'm doing, for just me.
It doesn't truly mean grows I've done completely without some kind of contamination. I've done I think around 26-27 grows, and 2 of those grows have been contaminated to the point were I felt it warranted a toss. (Both trich). So I guess more like 93% success.
Anything that generally bears fruit for me does pretty well...
By the way, Azur, that lab is tight as fuck.
-------------------- Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup. OR... You should be cultivating reptiles and fish, not mushrooms.
  
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Thanks mang. It makes it nice having a dedicated area to work in without having to wash breakfast dishes before working or kicking the dog out.
I don't keep track of contam rates. The biggest thing is trying to ensure you have clean spawn. Then everything should crop out just fine.
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