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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Clean room [Re: azur]
#23799836 - 11/04/16 06:41 AM (7 years, 2 months ago) |
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need a paper weight for those parafilm strips too
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Mushierage
SWIM Sinker



Registered: 06/29/16
Posts: 1,094
Last seen: 7 years, 1 month
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I don't feel any need for a clean room. I barely have any contamination as is, and when I do, I usually am expecting it because of something I know I did incorrectly, or not with care.
I just spray a bit of Lysol around my room and inside my sab, let the air settle for about an hour, and go into that room with no moving air, and do my business. When I'm doing clean work (inoculating, agar, transfers, etc) generally I'll try to do all those things all at once, so I'll consolidate my work in one small area of the room and make sure everything is quickly accessible to me, that way I'm not getting up constantly and kicking around air with potential contaminants in it.
I have a 95% success rate nearly with my methods. If I were to clamp down on every clean step I could, and be really anal about it, I could get that up to 99% pretty easily.
It doesn't take a mad scientist lab and a degree in mycology to be really successful at this. All you really need to do is practice the sterile tek steps that are most important, like using an SAB, letting air settle, flaming tools, using gloves... just use common sense.
In every cubic inch of air you breath, there are thousands of contaminants floating around. The only way to get away from them it to literally let them settle out of the air, and inoculate when they aren't floating around. (Aka inside your SAB after the air has settled. It's common sense).
-------------------- Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup. OR... You should be cultivating reptiles and fish, not mushrooms.
  
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Is it truly 95%? Of 89%? I'm genuinely curious. And what do you base that on to get a percentage? A lot of guys on here say the same thing and it never made sense. How does one figure the success rate? Is it with tubs only? Or jars and plates too?
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morty422
Wuba-luba-dub-dub!


Registered: 07/06/16
Posts: 988
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Quote:
Mushierage said: I don't feel any need for a clean room. I barely have any contamination as is, and when I do, I usually am expecting it because of something I know I did incorrectly, or not with care.
I just spray a bit of Lysol around my room and inside my sab, let the air settle for about an hour, and go into that room with no moving air, and do my business. When I'm doing clean work (inoculating, agar, transfers, etc) generally I'll try to do all those things all at once, so I'll consolidate my work in one small area of the room and make sure everything is quickly accessible to me, that way I'm not getting up constantly and kicking around air with potential contaminants in it.
I have a 95% success rate nearly with my methods. If I were to clamp down on every clean step I could, and be really anal about it, I could get that up to 99% pretty easily.
It doesn't take a mad scientist lab and a degree in mycology to be really successful at this. All you really need to do is practice the sterile tek steps that are most important, like using an SAB, letting air settle, flaming tools, using gloves... just use common sense.
In every cubic inch of air you breath, there are thousands of contaminants floating around. The only way to get away from them it to literally let them settle out of the air, and inoculate when they aren't floating around. (Aka inside your SAB after the air has settled. It's common sense).

I haven't gotten a contamination in a jar or in a clean agar transfer in a long while... I do exactly what mushierage does. 
The only time I get a contaminate is when I know I will.
"oops...dropped that wedge on the ground... eh... 5 second rule..."
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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I lost a 40 jar run of masters recently. Was in a hurry to get out and cut my cook time short. Contamination is part of this game. You'll never not have any. Just doesn't work that way. Mushierage, I'm a lysol fan too.
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Clean room [Re: azur]
#23800066 - 11/04/16 08:42 AM (7 years, 2 months ago) |
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oh man, the shroomery's entertaining  spraying a surface disinfectant in the air, talking about common sense in the same post!
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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I didn't say if I spray it in the air or not. Although, one would think it would certainly help particles in the air fall. Don't hate cause you can't afford lysol
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Clean room [Re: azur]
#23800087 - 11/04/16 08:50 AM (7 years, 2 months ago) |
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if you didnt say it then perhaps I wasn't talking about you then? :plotthickens:
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Your reply was to me though.... I do use it to wipe down the bottom of my fh. And I use lysol wipes to clean my masters before g2ging. And I did use the lysol air shit when I had a still air box. And if I had to go back to a still air box, I would use it again
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Clean room [Re: azur]
#23800101 - 11/04/16 08:56 AM (7 years, 2 months ago) |
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ah, reply fail confusion. how about some "common sense"? :P
but yeah surface disinfectants are great for disinfecting surfaces as you say. But spraying the room you set up your SAB in with lysol is a waste of lysol.
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Common sense? What is that?
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Clean room [Re: azur] 1
#23800128 - 11/04/16 09:08 AM (7 years, 2 months ago) |
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Common sense aint common enough anymore (true quote).
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Spore Ninja
PsychoMycoPhile


Registered: 10/25/16
Posts: 161
Last seen: 5 years, 6 months
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Quote:
spore-ty said: Better explanation heres what I keep in here still working on it gonna add shelving to the walls to let jars colonize, paper towel holder, flowhood etc. But here it.is so far
[url=https://files.shroomery.org/files/16-44/819966187-20161103_145025.jpg][image]/forums/thumbs/16-44/819966187-
In a galaxy far far away...
I pulled the carpet out and laid down new, heavy duty linoleum flooring. Put heavy duty commercial rubber baseboards in. Painted the walls and ceiling with 2 coats of easy to wash flat white.
I had one clear tub for my clean surgical scrubs. Another upright cabinet for larger supplies, and a couple of rolling carts with sliding drawers for smaller supplies like gloves, masks, syringes etc..
I had a table with the flow hood, and a separate 4ftX4ft space framed out in PVC pipe and covered in 6mil clear plastic sheeting as sort of an 'isolation' area. SAB, poured plates works in progress stayed in there, separate from the work/grow area. The idea being, if a tub contamed, it hopefully wouldn't be able to spread to the isolated mini clean room.
Big round Honeywell HEPA's on the floor ran 24/7 unless the flowhood was on. Incoming AC vents were filtered. A small dorm fridge in the room held samples.
OCD is a lifestyle, not a hobby.
-------------------- I'm interested in Dr. Pollock's work and his (especially older) strains. Anyone have pix of his Shroommobile RV?? PSA: Protonmail offers free, encrypted email hosted in privacy loving Switzerland. Red Phone / Signal offer free encrypted phone calls and texting between users. All of these programs are available free for PC and Android or apple and are available in the respective stores or online by googling the names. Stay safe out there! The Electronic Freedom Foundation's review of text and messaging program's security is here>>> https://www.eff.org/node/82654
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Spore Ninja
PsychoMycoPhile


Registered: 10/25/16
Posts: 161
Last seen: 5 years, 6 months
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Quote:
spore-ty said: Better explanation heres what I keep in here still working on it gonna add shelving to the walls to let jars colonize, paper towel holder, flowhood etc. But here it.is so far

Awesome arm holes in that SAB...
-------------------- I'm interested in Dr. Pollock's work and his (especially older) strains. Anyone have pix of his Shroommobile RV?? PSA: Protonmail offers free, encrypted email hosted in privacy loving Switzerland. Red Phone / Signal offer free encrypted phone calls and texting between users. All of these programs are available free for PC and Android or apple and are available in the respective stores or online by googling the names. Stay safe out there! The Electronic Freedom Foundation's review of text and messaging program's security is here>>> https://www.eff.org/node/82654
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Dedication!
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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And some arm chafing. Those are the keys to epic cultivation
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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hehe, teaches you to keep you arms the fuck away from the SAB itself
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Marty Mycfly
Time Traveler


Registered: 12/16/13
Posts: 976
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Quote:
spore-ty said: Im asking more for the people who have a room for inoculation with a flowhood, stainless steel table and etc. Do you guys keep your clean spawn in there , do you keep substrate/spawn materials in there etc.
It's always best have a separate incubation area for spawn and sub, however I don't and does cause some issues, a room packed with sub get's super warm and running the hood makes it even warmer, and then it gets super hot when you are cooling sub or spawn in there. I suggest if your designing a spot, keep in mind an incubation area as well that is not in your lab. Here is a kind of 360 view of my lab/incubation area
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 8 hours, 21 minutes
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Quote:
Marty Mycfly said:
Quote:
spore-ty said: Im asking more for the people who have a room for inoculation with a flowhood, stainless steel table and etc. Do you guys keep your clean spawn in there , do you keep substrate/spawn materials in there etc.
It's always best have a separate incubation area for spawn and sub, however I don't and does cause some issues, a room packed with sub get's super warm and running the hood makes it even warmer, and then it gets super hot when you are cooling sub or spawn in there. I suggest if your designing a spot, keep in mind an incubation area as well that is not in your lab. Here is a kind of 360 view of my lab/incubation area

-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
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That's fucking awesome!
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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