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helmerboing



Registered: 10/20/16
Posts: 18
Last seen: 6 years, 9 months
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Newb Rye Berry and Pasty Plate Inoculation
#23790300 - 11/01/16 01:27 AM (7 years, 2 months ago) |
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First of all, I sure do appreciate all the knowledge and entertainment this community has provided me as I lurked. Thanks!
TL;DR version - Look at these pics of stuff I did.
I'm in the process of my first attempt. I wanted to share where I'm at and where I hope to be with anyone interested (altruistically of course - definitely not hoping for tips and advice ).
I prepared 14 Quart jar lids with 2 holes, rtv ship and tight poly-fil. I rinsed and soaked rye berries in 50/50 hot water/weak coffee. Simmered for 20 minutes. I strained and tossed the berries and then spread them thin on a newspaper covered table. Once the berries passed the toilet paper test the jars were loaded up, foiled and pressure canned at 15 psi for 90 minutes.
I set up a SAB in a small closet and tried to sterilize the best I could. A serious amount of Lysol and 70% Iso happened rofl. 7 jars were inoculated with a ms syringe of Corumba Brazil and 7 with a syringe of PE.
Finally, I followed Pastywhyte'sEasy Agar Tek to give it a try. Half got a drop of CB; half PE.
Working towards a monotub for each strain. Pasteurized c/v/g is the plan but open to additives (e.g., coffee and worm casings).
I'm very aware of how little I know about this stuff so feel free to light me up if I'm doing something stoopid.

Doggo tax
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Experience is the comb we get after we lose our hair.
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 11 hours
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Re: Newb Rye Berry and Pasty Plate Inoculation [Re: helmerboing]
#23790340 - 11/01/16 02:23 AM (7 years, 2 months ago) |
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Looks pro level bro......nice
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: Newb Rye Berry and Pasty Plate Inoculation [Re: Edmunter]
#23790721 - 11/01/16 09:03 AM (7 years, 2 months ago) |
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Looks nice. Wetting your sab walls try using soapy water in a spray bottle instead of ISO.
When I do rhy its a 12-24 hour soak then heated up to ~200*F then strained with a fan on it.
I like doing one plate to one jar. So say you do ten grain jars from agar, the two healthiest can be expanded while the slower 80% get spawned to a tub
killer thread
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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Huskies
Boop More Snoots



Registered: 03/22/16
Posts: 1,048
Last seen: 1 day, 7 hours
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Re: Newb Rye Berry and Pasty Plate Inoculation [Re: filthyknees]
#23790740 - 11/01/16 09:19 AM (7 years, 2 months ago) |
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I'd recommend not using additives for your first one, it'll just be extra vectors to potentially screw it up, just coir verm and maybe gypsum will be great enough if your jars are healthy.
Looks good though, start sectoring that agar sooner than later onto new plates, because I would not trust spores to agar to be contam free for future agar to grain or making LI.
-------------------- I call them Huskies cause you tell them to go "Mush! Mush""
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helmerboing



Registered: 10/20/16
Posts: 18
Last seen: 6 years, 9 months
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Re: Newb Rye Berry and Pasty Plate Inoculation [Re: helmerboing]
#23790924 - 11/01/16 10:33 AM (7 years, 2 months ago) |
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Thanks for the feedback y'all!
Soapy water makes sense, guessing it traps contams as opposed to killing them? I definitely could do with less fumes in small spaces set up to not have ventilation lol. When you heat the rye up do you go straight to high heat? I tried to bring them up to a simmer/boil gradually; I was hoping this would cut down on the berries that burst and get gross.
I mostly made the plates to practice the process; I'm surprised they've done this well. I've looked into sectoring and isolation enough to have a general understanding of the concept but the practical execution has me rather intimidated.
Filthyknees' idea on agar->grain transfers is pretty close to what I was thinking. 1 plate to 1 jar transfers. While those colonize I'd order some actual petri dishes (for ease of viewing). As soon as I had a fruit with desirable traits I would use some of its flesh on agar to clone it. Who knows, in the interim a laminar flow hood could fall off a truck in front of my apartment and really boost my confidence. Jk.
As for c/v/g, I agree with you, Huskies. Occam's Razor ftw. I'll keep it simple now and add variables as I gain experience.
Thanks again, I am super grateful for the help and direction!
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Experience is the comb we get after we lose our hair.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Newb Rye Berry and Pasty Plate Inoculation [Re: helmerboing]
#23790933 - 11/01/16 10:38 AM (7 years, 2 months ago) |
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You don't need to try to sanitize a SAB and you cannot sterilize one. It's about how they work and how you use it. Not how much lysol you spray. I only use a little 70% iso. On a paper towel to wipe tops of jars and a little for my hands/gloves
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Terpfreak
❀Terpenes❀



Registered: 07/08/16
Posts: 1,065
Loc: Land Of Ooo
Last seen: 5 years, 10 months
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Re: Newb Rye Berry and Pasty Plate Inoculation [Re: bodhisatta]
#23790995 - 11/01/16 11:04 AM (7 years, 2 months ago) |
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Hell Yea Way to go!
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Huskies
Boop More Snoots



Registered: 03/22/16
Posts: 1,048
Last seen: 1 day, 7 hours
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Re: Newb Rye Berry and Pasty Plate Inoculation [Re: Terpfreak]
#23791166 - 11/01/16 12:09 PM (7 years, 2 months ago) |
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Don't feel intimated by doing agar transfers.
Get an all metal exacto knife, pressure cook it whenever you do more plates and pick a nice little sector from the best areas, away from any bacterial contamination that might show up and you are good to go!
-------------------- I call them Huskies cause you tell them to go "Mush! Mush""
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Newb Rye Berry and Pasty Plate Inoculation [Re: Huskies]
#23791184 - 11/01/16 12:18 PM (7 years, 2 months ago) |
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I've never put my scalpel/exacto in the pressure cooker. You flame the blade anyway
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Frosted Flakes
THEY'RE GRRREAT!


Registered: 01/12/15
Posts: 153
Loc: In the pantry
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Re: Newb Rye Berry and Pasty Plate Inoculation [Re: bodhisatta]
#23791321 - 11/01/16 01:15 PM (7 years, 2 months ago) |
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Quote:
bodhisatta said: I've never put my scalpel/exacto in the pressure cooker. You flame the blade anyway
Totally. Only being able to do one or two transfers per scalpel would be tedious.
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My Grow Log
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Huskies
Boop More Snoots



Registered: 03/22/16
Posts: 1,048
Last seen: 1 day, 7 hours
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Re: Newb Rye Berry and Pasty Plate Inoculation [Re: Frosted Flakes]
#23791356 - 11/01/16 01:25 PM (7 years, 2 months ago) |
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Haha interesting. I mean I flame it between plates and everything, but I thought might as well bring a sterile instrument into the SAB. I wrap it in aluminum foil with jars or plates depending on what I do, and only open it up once my hands are in the SAB.
-------------------- I call them Huskies cause you tell them to go "Mush! Mush""
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 11 hours
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Re: Newb Rye Berry and Pasty Plate Inoculation [Re: Huskies]
#23791402 - 11/01/16 01:41 PM (7 years, 2 months ago) |
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Quote:
Huskies said: Haha interesting. I mean I flame it between plates and everything, but I thought might as well bring a sterile instrument into the SAB. I wrap it in aluminum foil with jars or plates depending on what I do, and only open it up once my hands are in the SAB.
I do this too, it doesnt hurt
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helmerboing



Registered: 10/20/16
Posts: 18
Last seen: 6 years, 9 months
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Re: Newb Rye Berry and Pasty Plate Inoculation [Re: Edmunter]
#23807112 - 11/06/16 03:24 PM (7 years, 2 months ago) |
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Heya,
I figured I'd post an update on the colonization.
I think these jars are ready to shake (Corumba Brazil if anyone was curious).

I transferred the agar plates I made to Rye jars. I didn't see any obvious contamination but regardless, I was just trying to get some practice.

Potato pupper tax.
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Experience is the comb we get after we lose our hair.
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Greg
always learning




Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 3 months, 3 days
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Re: Newb Rye Berry and Pasty Plate Inoculation [Re: helmerboing]
#23807127 - 11/06/16 03:31 PM (7 years, 2 months ago) |
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Quote:
helmerboing said:


Nice work man... Give those fuckers a good shake.
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Mushierage
SWIM Sinker



Registered: 06/29/16
Posts: 1,094
Last seen: 7 years, 1 month
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Re: Newb Rye Berry and Pasty Plate Inoculation [Re: helmerboing]
#23807129 - 11/06/16 03:31 PM (7 years, 2 months ago) |
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Next time, I would try a method called Tiger Dropping. It just involves cutting a hash like shape into your colonized agar, and dropping it into your jars as a load of separate pieces. The more inoculation points you have, the faster the colonization will be for that jar obviously.
Your jars look great, they are definitely ready for a shake shake
-------------------- Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup. OR... You should be cultivating reptiles and fish, not mushrooms.
  
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b1blazin
The Transcendent


Registered: 06/16/12
Posts: 399
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Re: Newb Rye Berry and Pasty Plate Inoculation [Re: Mushierage]
#23807176 - 11/06/16 03:46 PM (7 years, 2 months ago) |
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^^^this is what i do and ill throw a vote for blenderless LI as well. much faster
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Freemind777
Psychonaut
Registered: 10/09/14
Posts: 11
Last seen: 5 years, 7 months
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Re: Newb Rye Berry and Pasty Plate Inoculation [Re: b1blazin]
#23807263 - 11/06/16 04:15 PM (7 years, 2 months ago) |
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Shake this jars!
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helmerboing



Registered: 10/20/16
Posts: 18
Last seen: 6 years, 9 months
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Re: Newb Rye Berry and Pasty Plate Inoculation [Re: Freemind777]
#23827179 - 11/12/16 08:26 PM (7 years, 2 months ago) |
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It's been a week since I shook these jars. I think these 6 are ready to spawn, they've looked fully colonized for a few days. I've started preparing my cvg following Frank's Proper Pasteurization Tek 3.0. I doubled it up, 2 buckets each getting 1 brick coir / 2 qts coarse verm / 5 qts water. I'm guessing I'll wind up with about 20 qts of cvg. I made so much because of the next part.
  
A friend drilled holes in a 66qt Sterilite tote and mistakenly drilled the 4 side holes 5.5 inches from the bottom. The plan was for a depth of the 3-4 inches. Should I add more cvg and make it 5 inches deep or would a depth of 4 inches still work? Pasteurizing all that substrate is gonna suck lol.


Any direction would be appreciated, these 6 are first children.
The 7th sibling is really nice but I suspect there may be some issues.
  
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Experience is the comb we get after we lose our hair.
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
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Re: Newb Rye Berry and Pasty Plate Inoculation [Re: helmerboing]
#23827242 - 11/12/16 08:52 PM (7 years, 2 months ago) |
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The longer you wait, more bacteria you accumulate.

and what is this?
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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helmerboing



Registered: 10/20/16
Posts: 18
Last seen: 6 years, 9 months
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Re: Newb Rye Berry and Pasty Plate Inoculation [Re: Kenetic]
#23827406 - 11/12/16 09:40 PM (7 years, 2 months ago) |
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I had this sitting unopened so I pondered using it to pasteurize some jars. My pc can handle seven jars so I was thinking of trying the oven roaster at the same time. 6 spawn jars and 14 of the substrate for about 20 qts total. With the depth issue I'm not sure if 14 qts is enough so I might just do three runs of 7 in the pc following Frank's tek exactly. The oven roaster variable might not be worth adding.
How deep would you make the sub? Should the 7th jar get mixed in, spawned to it's own tub or trashed?
Thanks for the help, the time/contams advice is motivating me to be done with this before the weekend is out.
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Experience is the comb we get after we lose our hair.
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