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OfflineAgarStudent
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Cloning a Stone
    #23787576 - 10/31/16 05:53 AM (7 years, 2 months ago)

I have this one jar of Mexicana that has progressed really well, except I no longer have the agar plate that it came from.

I don't want to ruin the jar, but at the same time would like to take a sample of a stone and grow it out on agar to grow more jars the same?  I figure whatever sectors that are present in the jar should likely be present in the stone?

Is is possible to clone a stone and not ruin the jar if I do it over the flow hood, use a flame sterilised scalpel and put the lid back on afterwards?

Thanks guys


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Invisibleblindingleaf
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Re: Cloning a Stone [Re: AgarStudent]
    #23787580 - 10/31/16 05:57 AM (7 years, 2 months ago)

yea, possible (albeit weird) but depending on where the stone is in the jar….difficult.

hopefully its close to the top


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OfflineAgarStudent
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Re: Cloning a Stone [Re: blindingleaf]
    #23787583 - 10/31/16 06:00 AM (7 years, 2 months ago)

Yep there are stones everywhere:



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Offlineenlightenment
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Re: Cloning a Stone [Re: AgarStudent]
    #23787588 - 10/31/16 06:03 AM (7 years, 2 months ago)

Nice jar. Packet with sclerotia. You can wait until you harvest all and clone a stone at this moment.


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Invisibleblindingleaf
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Re: Cloning a Stone [Re: AgarStudent]
    #23787591 - 10/31/16 06:04 AM (7 years, 2 months ago)

go for it :thumbup:

TBH, u could just use a new scalpel blade and kinda "slice" a piece of one of the stones on the glass. its sterile, so u don't need to worry about the outside of the "fruit body" being contaminated


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

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OfflineAgarStudent
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Re: Cloning a Stone [Re: blindingleaf]
    #23787597 - 10/31/16 06:09 AM (7 years, 2 months ago)

Okay, thanks guys.
Will give it a go :smile:


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InvisibleLocN9ne
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Re: Cloning a Stone [Re: AgarStudent]
    #23787623 - 10/31/16 06:26 AM (7 years, 2 months ago)

You dont even have to dig a stone out... Stab a piece of grain and throw that bitch onto a plate... But either way is fine.


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Offlinesandy_vag
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Re: Cloning a Stone [Re: LocN9ne]
    #23787978 - 10/31/16 09:39 AM (7 years, 2 months ago)

Anybody seen mexicana A-strain spores around? Can't locate them at any USA vendors.


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InvisibleLocN9ne
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Re: Cloning a Stone [Re: sandy_vag]
    #23788240 - 10/31/16 11:10 AM (7 years, 2 months ago)

Atl7? It'll be called galindoi


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Q&A
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Edited by LocN9ne (10/31/16 11:10 AM)


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Offlineoakley
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Re: Cloning a Stone [Re: LocN9ne]
    #23788257 - 10/31/16 11:19 AM (7 years, 2 months ago)

If you used an isolate from the agar plate you can just drop any grain from your jar on agar since all the mycelium in the jar would have grown from a single genetic lineage.


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InvisibleLocN9ne
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Re: Cloning a Stone [Re: oakley]
    #23788319 - 10/31/16 11:44 AM (7 years, 2 months ago)

True, and on the other end of the spectrum if it was from MS the diversity would be so great that you will get pretty much around the same results with the same single grain method you just mentioned... Isolates have a place, but they are over rated by folks until they learn quite a bit more... Isolates arent anywhere near being in my arsenal yet... Too much other shit to nail down first,


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OfflineSpore Ninja
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Re: Cloning a Stone [Re: sandy_vag]
    #23788453 - 10/31/16 12:30 PM (7 years, 2 months ago)

Quote:

sandy_vag said:
Anybody seen mexicana A-strain spores around? Can't locate them at any USA vendors.




I believe Sporeworks was the originator of the Mexi A line, so I'd go ahead and invest in the good genetics they have.

Plan B would be to order some truffle from one of our vendors that offers them, and then clone them. That's what I'm doing to get some good Tamapanenis genetics.
It's about $20 if you don't use the registered/tracked shipping option- $40 if you do.


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Offlineblackout
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Re: Cloning a Stone [Re: Spore Ninja]
    #23788768 - 10/31/16 02:26 PM (7 years, 2 months ago)

Quote:

Spore Ninja said:
Quote:

sandy_vag said:
Anybody seen mexicana A-strain spores around? Can't locate them at any USA vendors.




I believe Sporeworks was the originator of the Mexi A line, so I'd go ahead and invest in the good genetics they have.



+1

Quote:

Spore Ninja said:
Plan B would be to order some truffle from one of our vendors that offers them, and then clone them.



I presume he is in the US if looking for US vendors. I would not be ordering truffles to the US, I thought no shroomery sponsors would send truffles to the US either. I meant to mention it in your other thread.


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Offlinesandy_vag
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Re: Cloning a Stone [Re: blackout]
    #23788799 - 10/31/16 02:34 PM (7 years, 2 months ago)

Yea I been checking sporeworks for over a year now and they've always been out of stock on the mexicana a-strain. Even sent an email to ask if they would get any more but never heard back.


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OfflineAgarStudent
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Re: Cloning a Stone [Re: sandy_vag]
    #23789409 - 10/31/16 06:00 PM (7 years, 2 months ago)

Ah yeah I have no idea if it is an isolate in that jar or not.
I think RR mentioned that he would try to transfer the brown bits for better stones:

Quote:

RogerRabbit said:
The darker parts produce more stones.  This is just an observation.  Isolate some of both to confirm.
RR




I guess if it grows out symmetrically and does not have sectors then it may be an isolate.  Still different sectors may work together to produce stones too.

I am confused a bit by the naming convention, but to get things straight:

Psilocybe Mexicana: Strain A = 'Chico Nindo' and;

Psilocybe Mexicana: Strain B = 'Jalisco'

Psilocybe Galindoi: = ATL#7

Could anyone clarify that this is right or if there are any bits I have missed? :tongue:

One other thing..
What do you do with jars like this that are about three months old, with different genetics and still look kinda crappy?  Do you try and fruit them or leave them?



Cheers


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Why reinvent the wheel when someone here has already done the science?



Edited by AgarStudent (10/31/16 06:44 PM)


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InvisibleLocN9ne
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Re: Cloning a Stone [Re: AgarStudent]
    #23789622 - 10/31/16 07:13 PM (7 years, 2 months ago)

That jar is fucked with bacteria, i dont see a single stone in there... You could attempt to spawn it, but three months of bacteria breeding is not a promising prospect.


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OfflineAgarStudent
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Re: Cloning a Stone [Re: LocN9ne]
    #23789665 - 10/31/16 07:29 PM (7 years, 2 months ago)

Alright, that sounds possible.
Maybe the brown bits are really tiny stones trying to co-exist with the bacteria and somehow they have achieved a balance?
Still I guess it is not useful for anything but the compost bin? :confused:



Edited by AgarStudent (11/01/16 06:03 AM)


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Offlineoakley
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Re: Cloning a Stone [Re: AgarStudent]
    #23790452 - 11/01/16 05:11 AM (7 years, 2 months ago)

Quote:

AgarStudent said:
I am confused a bit by the naming convention, but to get things straight:

Psilocybe Mexicana: Strain A = 'Chico Nindo' and;

Psilocybe Mexicana: Strain B = 'Jalisco'

Psilocybe Galindoi: = ATL#7

Could anyone clarify that this is right or if there are any bits I have missed? :tongue:




This is absolutely not correct. Mex-A and Mex-B have been isolated by workman I think, and jalisco have been domesticated by workman

https://www.shroomery.org/forums/showflat.php/Number/4153712

chicon nindo was collected by azure and distributed by azure/elfstone. the thread is here on shroomery.

https://www.shroomery.org/forums/showflat.php/Number/15954278

additionally there is also a lox strain going around collected by alan rockerfeller and distributed by prof pinhead.


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InvisibleLocN9ne
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Re: Cloning a Stone [Re: oakley]
    #23792427 - 11/01/16 06:32 PM (7 years, 2 months ago)

Quote:

oakley said:
Quote:

AgarStudent said:
I am confused a bit by the naming convention, but to get things straight:

Psilocybe Mexicana: Strain A = 'Chico Nindo' and;

Psilocybe Mexicana: Strain B = 'Jalisco'

Psilocybe Galindoi: = ATL#7

Could anyone clarify that this is right or if there are any bits I have missed? :tongue:




This is absolutely not correct. Mex-A and Mex-B have been isolated by workman I think, and jalisco have been domesticated by workman

https://www.shroomery.org/forums/showflat.php/Number/4153712

chicon nindo was collected by azure and distributed by azure/elfstone. the thread is here on shroomery.

https://www.shroomery.org/forums/showflat.php/Number/15954278

additionally there is also a lox strain going around collected by alan rockerfeller and distributed by prof pinhead.



Your ability to dig up specific relics on demand, and name drop... Combined with your post count and reg date really got me wondering who is pulling your strings there oakley


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OfflineAgarStudent
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Re: Cloning a Stone [Re: LocN9ne]
    #23792447 - 11/01/16 06:38 PM (7 years, 2 months ago)

With strain isolation, it looks like Chico is outperforming Galindoi :wink:


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