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InvisibleDavidReishi
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How can I start a non-sterile culture without stem-butts?
    #23787075 - 10/30/16 10:07 PM (7 years, 2 months ago)

I have a plastic bag in the fridge with some Azures cut less than 36 hours ago. I didn't bring home any stem butts. Is there anything simple I can do to get some non-sterile cultures going?


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Species found in the Bay area: 
P. allenii, P. cyanescens, P. ovoideocystidiata, P. stuntzii, P. azurescens


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OfflineHuskies
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Re: How can I start a non-sterile culture without stem-butts? [Re: DavidReishi]
    #23787119 - 10/30/16 10:26 PM (7 years, 2 months ago)

You can in theory use any part of the mushroom. Try a sample from the stem.


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I call them Huskies cause you tell them to go "Mush! Mush""


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Offlinedextr0
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Re: How can I start a non-sterile culture without stem-butts? [Re: DavidReishi]
    #23787123 - 10/30/16 10:28 PM (7 years, 2 months ago)

Clone to agar.


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OfflinePsilosopherr
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Re: How can I start a non-sterile culture without stem-butts? [Re: dextr0]
    #23787154 - 10/30/16 10:49 PM (7 years, 2 months ago)

spores baby. :cool2:

Would need cleaning up on agar to get a clean culture.


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Invisibledankington
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Re: How can I start a non-sterile culture without stem-butts? [Re: Psilosopherr]
    #23787159 - 10/30/16 10:52 PM (7 years, 2 months ago)

He can likely take a few stem butts, blend them up with water and use the slurry on wood chips, actually.


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OfflinePsilosopherr
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Re: How can I start a non-sterile culture without stem-butts? [Re: dankington]
    #23787166 - 10/30/16 10:55 PM (7 years, 2 months ago)

whole point of the thread is to not use stem buts, actually :wink: :grin:


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Invisibledankington
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Re: How can I start a non-sterile culture without stem-butts? [Re: Psilosopherr]
    #23787183 - 10/30/16 11:05 PM (7 years, 2 months ago)

and yet, he has a bag full of azures in his fridge :shrug: kinda silly


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OfflineMushierage
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Re: How can I start a non-sterile culture without stem-butts? [Re: dankington]
    #23787198 - 10/30/16 11:16 PM (7 years, 2 months ago)

Have any extra pins?  Just use the entire thing.  Boom.


--------------------
Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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Invisibledankington
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Re: How can I start a non-sterile culture without stem-butts? [Re: Mushierage]
    #23787203 - 10/30/16 11:19 PM (7 years, 2 months ago)

well, but how does he have a bag of azzies and no stem to make a slurry from? I think OP was simply too literal with the term "stem butts". Also, a plastic bag in the fridge? You should have used a paper bag, so they're probably kinda gross by now.


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Invisiblefreespeech
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Re: How can I start a non-sterile culture without stem-butts? [Re: dankington]
    #23787231 - 10/30/16 11:35 PM (7 years, 2 months ago)

I've prepared a gallon bucket of soaked woodchips (with a tiny bit of bleach included in the soak) and inoculated it with cyanescens mycelium chips that I dug from a local outdoor patch. This was successful; the entire bucket colonized beautifully. You could try something similar with some of the stems from your haul, but I doubt you're going to get anything good going with nasty wet stems that have spent a day and a half in your fridge in a plastic bag.


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InvisibleDavidReishi
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Re: How can I start a non-sterile culture without stem-butts? [Re: freespeech]
    #23787294 - 10/31/16 12:05 AM (7 years, 2 months ago)

Thanks, guys. Btw, inside a plastic bag in the fridge is how you keep fresh mushrooms fresh. A paper bag would never work...the mushies would dry out.


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Species found in the Bay area: 
P. allenii, P. cyanescens, P. ovoideocystidiata, P. stuntzii, P. azurescens


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Invisiblefreespeech
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Re: How can I start a non-sterile culture without stem-butts? [Re: DavidReishi]
    #23787302 - 10/31/16 12:07 AM (7 years, 2 months ago)

Kinda depends on how wet they are when you put them in there. Fresh mushrooms picked when it's wet outside tend to get slimy and gross in a closed plastic bag in the fridge. I'd rather they dry out a little bit than get slimy. It's not like they're not going to dehydrate in a paper bag in the fridge. Fresh air is a friend to mushrooms.


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InvisibleDavidReishi
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Re: How can I start a non-sterile culture without stem-butts? [Re: freespeech]
    #23787310 - 10/31/16 12:11 AM (7 years, 2 months ago)

Quote:

freespeech said:
It's not like they're not going to dehydrate in a paper bag in the fridge.




Actually they do, but that's besides the point.


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Species found in the Bay area: 
P. allenii, P. cyanescens, P. ovoideocystidiata, P. stuntzii, P. azurescens


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Invisiblefreespeech
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Re: How can I start a non-sterile culture without stem-butts? [Re: DavidReishi]
    #23787314 - 10/31/16 12:13 AM (7 years, 2 months ago)

Right, if you want to get technical about it, sure. I said they will dry out a little bit, but by "dehydrate," I mean they won't get close to cracker dry any time soon. I'm guessing they would retain most of their moisture for several days at least.


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InvisibleDavidReishi
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Re: How can I start a non-sterile culture without stem-butts? [Re: freespeech]
    #23787316 - 10/31/16 12:15 AM (7 years, 2 months ago)

True.


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Species found in the Bay area: 
P. allenii, P. cyanescens, P. ovoideocystidiata, P. stuntzii, P. azurescens


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InvisibleLocN9ne
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Re: How can I start a non-sterile culture without stem-butts? [Re: DavidReishi]
    #23787346 - 10/31/16 12:40 AM (7 years, 2 months ago)

They are going to get nasty quick... A paper or cloth bag or just leave them sitting out... But never a plastic bag... Thats bad form... And idk what you think a stem butt is, but you can use the whole mushroom... Pick a part and call it a stem butt... If you want to, throw a piece of the stem in some hydrated cardboard let it grow a bit then use that to noc up some chips...

Ol' boy with the bleach in his bucket, the bleach didnt do anything fyi.


--------------------


Q&A
US vs. THEM

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InvisibleDavidReishi
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Re: How can I start a non-sterile culture without stem-butts? [Re: LocN9ne]
    #23787374 - 10/31/16 01:01 AM (7 years, 2 months ago)

Quote:

LocN9ne said:
They are going to get nasty quick... A paper or cloth bag or just leave them sitting out... But never a plastic bag... Thats bad form...




Nonsense when it comes to the fridge...but ok, thanks.


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Species found in the Bay area: 
P. allenii, P. cyanescens, P. ovoideocystidiata, P. stuntzii, P. azurescens


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Offlineoakley
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Re: How can I start a non-sterile culture without stem-butts? [Re: DavidReishi]
    #23787388 - 10/31/16 01:10 AM (7 years, 2 months ago)

From stamets mushroom cultivator

The cap, the upper region of the stem and/or the
area where the gill plate joins the underside of the cap are the best locations for excising clean tissue.
Some mushrooms have a thick cuticle overlaying the cap. This skin can be peeled back and a tissue
culture can be taken from the flesh underlying it. Wipe the surface of the mushroom with a cotton
swab soaked in alcohol and remove any dirt or damaged external tissue. Break the mushroom cap
or stem, exposing the interior hyphae. Immediately flame a scalpel until red-hot and cool in a media
filled petri dish. Now cut into the flesh removing a small fragment of tissue. Transfer the tissue fragment to the center of the nutrient filled petri dish as quickly as possible, exposing the tissue and agar
to the open air for a minimal time. Repeat this technique into at least three, preferably five more
dishes. Label each dish with the species, date, type of culture (tissue) and kind of agar medium. If
successful, mycelial growth will be evident in three to seven days.

Also I can say from experience that any kind of mushroom stored in a plastic bag in the fridge will get moldy pretty soon.


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OfflineWhyDidiDoThis
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Re: How can I start a non-sterile culture without stem-butts? [Re: DavidReishi]
    #23787417 - 10/31/16 01:33 AM (7 years, 2 months ago)

Quote:

DavidReishi said:
Thanks, guys. Btw, inside a plastic bag in the fridge is how you keep fresh mushrooms fresh. A paper bag would never work...the mushies would dry out.




At work in the kitchen we must not wrap or seal the fresh culinary mushrooms.
In the walk-in fridge.
Keep the bag open to breathe or face rotting.


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Offlinemolemole
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Re: How can I start a non-sterile culture without stem-butts? [Re: WhyDidiDoThis]
    #23787468 - 10/31/16 02:40 AM (7 years, 2 months ago)

Couple weeks ago, i printed some azures on tinfoil. I ussually print in a Tupperware and mist the sides and lid. I left these caps to print for 2 days. When i pulled the caps all 3 where growing myc right where the stem was cut at. I put these to unsterized, unpasturized soaked wood chips in a gallon zip loc. Leave top cracked open a 1/4 to 1/2 way. Poke some hole in the bottom for drainage. Stems will just rot in the chips unless they have the stem butt with them.


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Edited by molemole (10/31/16 02:41 AM)


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InvisibleLocN9ne
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Re: How can I start a non-sterile culture without stem-butts? [Re: molemole]
    #23787472 - 10/31/16 02:42 AM (7 years, 2 months ago)

Side busting real quick...

Why did you mist the inside of the tupperware you were printing in?


--------------------


Q&A
US vs. THEM

The more I learn, the less I know.


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Offlinemolemole
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Re: How can I start a non-sterile culture without stem-butts? [Re: LocN9ne]
    #23787476 - 10/31/16 02:48 AM (7 years, 2 months ago)

I always do that, wild fruits are often dry and don't want to drop spores. First time i have seen myc take off like this, never seen this with cyans. But i ussually print for 24 hours.


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InvisibleLocN9ne
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Re: How can I start a non-sterile culture without stem-butts? [Re: molemole]
    #23787491 - 10/31/16 03:16 AM (7 years, 2 months ago)

:takingnotes:


--------------------


Q&A
US vs. THEM

The more I learn, the less I know.


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Offlinewtfcrazymofo
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Re: How can I start a non-sterile culture without stem-butts? [Re: DavidReishi]
    #23787501 - 10/31/16 03:35 AM (7 years, 2 months ago)

Op just take one of your least slimey mushrooms out of your plastic bag in the fridge, inside you s.a.b. tear open the stem scrape a tiny piece (rice grain sized) with your flamed scalpel and drop it on some agar.
Do several like this and see what grows.  Then take some healthy transfers.


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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InvisibleLocN9ne
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Re: How can I start a non-sterile culture without stem-butts? [Re: wtfcrazymofo]
    #23787619 - 10/31/16 06:23 AM (7 years, 2 months ago)

I'm lost... OP, what is your goal here? Why did non-sterile come into play? Were you just planning on spreading the stem butts to another location or are you wanting to start like an outdoor patch at your house?


--------------------


Q&A
US vs. THEM

The more I learn, the less I know.


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InvisibleDavidReishi
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Re: How can I start a non-sterile culture without stem-butts? [Re: LocN9ne]
    #23787784 - 10/31/16 08:30 AM (7 years, 2 months ago)

Quote:

molemole said:
Stems will just rot in the chips unless they have the stem butt with them.




That's the issue here.



Quote:

LocN9ne said:
I'm lost... OP, what is your goal here? Why did non-sterile come into play? Were you just planning on spreading the stem butts to another location or are you wanting to start like an outdoor patch at your house?




Non-sterile comes into play because I don't have access to sterile conditions, agar, etc. I'm not sure of my plans yet, but I've got some fresh Azzies and I wanna do something like stem-butt tek, but I kept no stem-butts.


--------------------
Species found in the Bay area: 
P. allenii, P. cyanescens, P. ovoideocystidiata, P. stuntzii, P. azurescens


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InvisibleLocN9ne
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Re: How can I start a non-sterile culture without stem-butts? [Re: DavidReishi] * 1
    #23787791 - 10/31/16 08:37 AM (7 years, 2 months ago)

Stem butts are just that, the butt of the stem... If they were something special we wouldnt be able to clone from the rest of the stipe... Hydrate some corregated cardboard throw a few pieces of stem in there, meh... A quick search will give you more details...


--------------------


Q&A
US vs. THEM

The more I learn, the less I know.


Edited by LocN9ne (10/31/16 08:37 AM)


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InvisibleDavidReishi
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Re: How can I start a non-sterile culture without stem-butts? [Re: LocN9ne]
    #23787819 - 10/31/16 08:44 AM (7 years, 2 months ago)

Dude I'm getting tired of your nasty mouth. I read the Wordsworthian Wanderer's piece about stem-butts to cardboard/woodchips, he mentions the importance of the mycelial butt of the stem that you have to pull up, I don't have any, so I went to private message him but he's got a note saying to make a new thread about any cultivation questions. So I did that. But so far...


--------------------
Species found in the Bay area: 
P. allenii, P. cyanescens, P. ovoideocystidiata, P. stuntzii, P. azurescens


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InvisibleDavidReishi
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Re: How can I start a non-sterile culture without stem-butts? [Re: DavidReishi]
    #23787953 - 10/31/16 09:31 AM (7 years, 2 months ago)

And please, people! Enough with the nonsense about storing fresh Psilocybes. Here's fresh Azures as of five minutes ago, after being stored in a plastic bag in the fridge for over 36 hours. They look as good as when I clipped 'em...even a little less wet.



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Species found in the Bay area: 
P. allenii, P. cyanescens, P. ovoideocystidiata, P. stuntzii, P. azurescens


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Invisibledankington
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Re: How can I start a non-sterile culture without stem-butts? [Re: DavidReishi]
    #23787992 - 10/31/16 09:46 AM (7 years, 2 months ago)

don't get your knickers in a twist, we're trying to help
anyone that knows anything about mushrooms knows not to store them in plastic in the fridge, and not to freeze fresh mushrooms. A paper bag in the fridge is the best way, if you're not going to dehydrate them right away.

ANY PART OF THE STEM WILL WORK. You just need live tissue to make a "stem butt slurry". If it didn't work, like loc said, I wouldn't be able to clone live tissue from up the stem by the cap (where I usually take my biopsy from).

So get to it :dancer:


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InvisiblebodhisattaMDiscordReddit
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Re: How can I start a non-sterile culture without stem-butts? [Re: dankington]
    #23787999 - 10/31/16 09:49 AM (7 years, 2 months ago)

Sounds like you're clueless op. :shrug:

N9ne hit it on the head


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InvisibleSirtalis
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Re: How can I start a non-sterile culture without stem-butts? [Re: dankington]
    #23788005 - 10/31/16 09:53 AM (7 years, 2 months ago)

I started my backyard grow with whole mushrooms like the ones in your photos.  No need for stem butts.  If you can sacrifice one or two, blend it up in a blender and pour it into a container with sawdust and scotts earthgro woodchips.  The mycelium will colonize the container. 

All you have to do is soak the sawdust/woodchips over night.  They key is to have a mix of very small pieces (sawdust) along with bigger chunks (woodchips). Drain it out and then pour your blend on it.  Mix it up and cover the bin with soaked cardboard to prevent moisture from escaping.  Make sure this bin has holes in it and is outside.  The mycelium needs fresh air in order to fend off bacteria and other molds.

If you don't feel like taking the stem to agar, this is your best bet.  To increase your odds of colonization use a few mushrooms.  The more the better.  If you want to guarantee success you'll need to go agar --> grains --> woodchips in sterile conditions.  BUT plenty of people have had success throwing ENTIRE mushrooms into piles of woodchips.  Including myself.  Look at how the mycelium grows off the stem onto the woodchips in this photo. 


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InvisibleDavidReishi
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Re: How can I start a non-sterile culture without stem-butts? [Re: Sirtalis]
    #23788012 - 10/31/16 09:57 AM (7 years, 2 months ago)

Thanks, Sirtalis! Help without the nastiness!


--------------------
Species found in the Bay area: 
P. allenii, P. cyanescens, P. ovoideocystidiata, P. stuntzii, P. azurescens


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InvisibleDavidReishi
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Re: How can I start a non-sterile culture without stem-butts? [Re: dankington]
    #23788030 - 10/31/16 10:03 AM (7 years, 2 months ago)

Quote:

dankington said:
don't get your knickers in a twist, we're trying to help
anyone that knows anything about mushrooms knows not to store them in plastic in the fridge




I don't want help from anyone that has to accompany it with nastiness. And about storing fresh Psilocybes, obviously my photo alone disproves "anyone who knows anything about mushrooms."


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Species found in the Bay area: 
P. allenii, P. cyanescens, P. ovoideocystidiata, P. stuntzii, P. azurescens


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Invisibledankington
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Re: How can I start a non-sterile culture without stem-butts? [Re: DavidReishi]
    #23788040 - 10/31/16 10:06 AM (7 years, 2 months ago)

That's funny. So you thought we were being nasty, but in reality you were being needlessly stubborn about things that you know nothing about and other things that didn't matter. I wonder why you opted out of ratings…?

:cookiemonster:

edit: ahh, mediocrity extraordinaire? Sounds accurate. Set the bar high, friend.


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InvisibleDavidReishi
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Re: How can I start a non-sterile culture without stem-butts? [Re: dankington]
    #23788059 - 10/31/16 10:10 AM (7 years, 2 months ago)

You do realize that we have an 18 and older policy here, don't you?


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Species found in the Bay area: 
P. allenii, P. cyanescens, P. ovoideocystidiata, P. stuntzii, P. azurescens


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Invisibledankington
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Re: How can I start a non-sterile culture without stem-butts? [Re: DavidReishi]
    #23788067 - 10/31/16 10:13 AM (7 years, 2 months ago)

really? I could probably be your father, broseph.


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Offlinespacechildo
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Re: How can I start a non-sterile culture without stem-butts? [Re: dankington]
    #23788069 - 10/31/16 10:13 AM (7 years, 2 months ago)

of course you dont store fresh mushrooms in plastic bags, come on. everyone who's done it knows why not. they wont be fine for much longer than a cpl days.

and :whathesaid: to everyone that said any part of the mushroom works for cloning.


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Re: How can I start a non-sterile culture without stem-butts? [Re: DavidReishi]
    #23788070 - 10/31/16 10:14 AM (7 years, 2 months ago)

This is the outdoor cultivation forum:

https://www.shroomery.org/forums/postlist.php/Board/296


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OfflinePsilosopherr
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Re: How can I start a non-sterile culture without stem-butts? [Re: Sirtalis]
    #23788185 - 10/31/16 10:54 AM (7 years, 2 months ago)

Why are we even trying to help this guy anymore? He clearly has all the answers.


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Invisibleazur
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Re: How can I start a non-sterile culture without stem-butts? [Re: spacechildo]
    #23788202 - 10/31/16 11:00 AM (7 years, 2 months ago)

I store mushrooms I want to clone in a plastic ziploc find the fridge for a day or three if I can't get to it right away :shrug:


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InvisibleLocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ
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Re: How can I start a non-sterile culture without stem-butts? [Re: Psilosopherr]
    #23788231 - 10/31/16 11:09 AM (7 years, 2 months ago)

Desiccant chamber, fish tank pump, plastic bags, and a microwave is all you need homie... The equipment to match your knowledge and attitude... If i had noticed you were an opt out i would have just ignored you like everyone else was, i usually catch the limp wristed ones... Im a little rusty, but its coming back... Best of luck little guy, hopefully you keep some butts next time because thats where the roots are, a little cloning powder and you just plant them...

Hey devils advocate, i mean Azur.., its weird seeing you post in cult.., i feel like i just spotted a unicorn in the wild.


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Offlinespacechildo
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Re: How can I start a non-sterile culture without stem-butts? [Re: azur]
    #23788235 - 10/31/16 11:09 AM (7 years, 2 months ago)

yeah a few days are OK but any more than that and you're left with a mess.

btw I thought zip locks were how you cloned? :tongue2: just take them out of the fridge when you're ready?


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Invisibleazur
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Re: How can I start a non-sterile culture without stem-butts? [Re: spacechildo]
    #23788342 - 10/31/16 11:51 AM (7 years, 2 months ago)

Quote:

LocN9ne said:

Hey devils advocate, i mean Azur.., its weird seeing you post in cult.., i feel like i just spotted a unicorn in the wild.



I'm sure cron will ban me soon...


Quote:

spacechildo said:

btw I thought zip locks were how you cloned? :tongue2: just take them out of the fridge when you're ready?



:highfive:


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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OfflineSloppyJoseph
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Re: How can I start a non-sterile culture without stem-butts? [Re: azur]
    #23788389 - 10/31/16 12:04 PM (7 years, 2 months ago)

Quote:

azur said:
I store mushrooms I want to clone in a plastic ziploc find the fridge for a day or three if I can't get to it right away :shrug:



Same dude. Fresh mushrooms are golden in plastic bags for a couple days. If you put a mushroom you want to clone in a paper bag it'll be worthless if you get to it in 3 days.


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InvisibleAdden
I'm a teapot
Registered: 06/04/03
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Re: How can I start a non-sterile culture without stem-butts? [Re: DavidReishi]
    #23790299 - 11/01/16 01:27 AM (7 years, 2 months ago)

I'm no rocket surgeon but if you're looking to start a stem butt project and don't have them, then get them.


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