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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: How can I start a non-sterile culture without stem-butts? [Re: molemole]
#23787472 - 10/31/16 02:42 AM (7 years, 2 months ago) |
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Side busting real quick...
Why did you mist the inside of the tupperware you were printing in?
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Q&A US vs. THEM The more I learn, the less I know.
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molemole
Ethnobotic



Registered: 04/14/12
Posts: 465
Loc: PNW
Last seen: 9 days, 14 hours
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Re: How can I start a non-sterile culture without stem-butts? [Re: LocN9ne]
#23787476 - 10/31/16 02:48 AM (7 years, 2 months ago) |
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I always do that, wild fruits are often dry and don't want to drop spores. First time i have seen myc take off like this, never seen this with cyans. But i ussually print for 24 hours.
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: How can I start a non-sterile culture without stem-butts? [Re: molemole]
#23787491 - 10/31/16 03:16 AM (7 years, 2 months ago) |
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Q&A US vs. THEM The more I learn, the less I know.
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 8 hours, 7 minutes
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Re: How can I start a non-sterile culture without stem-butts? [Re: DavidReishi]
#23787501 - 10/31/16 03:35 AM (7 years, 2 months ago) |
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Op just take one of your least slimey mushrooms out of your plastic bag in the fridge, inside you s.a.b. tear open the stem scrape a tiny piece (rice grain sized) with your flamed scalpel and drop it on some agar. Do several like this and see what grows. Then take some healthy transfers.
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: How can I start a non-sterile culture without stem-butts? [Re: wtfcrazymofo]
#23787619 - 10/31/16 06:23 AM (7 years, 2 months ago) |
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I'm lost... OP, what is your goal here? Why did non-sterile come into play? Were you just planning on spreading the stem butts to another location or are you wanting to start like an outdoor patch at your house?
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Q&A US vs. THEM The more I learn, the less I know.
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DavidReishi
Mediocrity Extraordinaire


Registered: 10/07/15
Posts: 1,333
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Re: How can I start a non-sterile culture without stem-butts? [Re: LocN9ne]
#23787784 - 10/31/16 08:30 AM (7 years, 2 months ago) |
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Quote:
molemole said: Stems will just rot in the chips unless they have the stem butt with them.
That's the issue here.
Quote:
LocN9ne said: I'm lost... OP, what is your goal here? Why did non-sterile come into play? Were you just planning on spreading the stem butts to another location or are you wanting to start like an outdoor patch at your house?
Non-sterile comes into play because I don't have access to sterile conditions, agar, etc. I'm not sure of my plans yet, but I've got some fresh Azzies and I wanna do something like stem-butt tek, but I kept no stem-butts.
-------------------- Species found in the Bay area: P. allenii, P. cyanescens, P. ovoideocystidiata, P. stuntzii, P. azurescens
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: How can I start a non-sterile culture without stem-butts? [Re: DavidReishi] 1
#23787791 - 10/31/16 08:37 AM (7 years, 2 months ago) |
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Stem butts are just that, the butt of the stem... If they were something special we wouldnt be able to clone from the rest of the stipe... Hydrate some corregated cardboard throw a few pieces of stem in there, meh... A quick search will give you more details...
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Q&A US vs. THEM The more I learn, the less I know.
Edited by LocN9ne (10/31/16 08:37 AM)
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DavidReishi
Mediocrity Extraordinaire


Registered: 10/07/15
Posts: 1,333
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Re: How can I start a non-sterile culture without stem-butts? [Re: LocN9ne]
#23787819 - 10/31/16 08:44 AM (7 years, 2 months ago) |
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Dude I'm getting tired of your nasty mouth. I read the Wordsworthian Wanderer's piece about stem-butts to cardboard/woodchips, he mentions the importance of the mycelial butt of the stem that you have to pull up, I don't have any, so I went to private message him but he's got a note saying to make a new thread about any cultivation questions. So I did that. But so far...
-------------------- Species found in the Bay area: P. allenii, P. cyanescens, P. ovoideocystidiata, P. stuntzii, P. azurescens
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DavidReishi
Mediocrity Extraordinaire


Registered: 10/07/15
Posts: 1,333
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Re: How can I start a non-sterile culture without stem-butts? [Re: DavidReishi]
#23787953 - 10/31/16 09:31 AM (7 years, 2 months ago) |
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And please, people! Enough with the nonsense about storing fresh Psilocybes. Here's fresh Azures as of five minutes ago, after being stored in a plastic bag in the fridge for over 36 hours. They look as good as when I clipped 'em...even a little less wet.
-------------------- Species found in the Bay area: P. allenii, P. cyanescens, P. ovoideocystidiata, P. stuntzii, P. azurescens
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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Re: How can I start a non-sterile culture without stem-butts? [Re: DavidReishi]
#23787992 - 10/31/16 09:46 AM (7 years, 2 months ago) |
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don't get your knickers in a twist, we're trying to help anyone that knows anything about mushrooms knows not to store them in plastic in the fridge, and not to freeze fresh mushrooms. A paper bag in the fridge is the best way, if you're not going to dehydrate them right away.
ANY PART OF THE STEM WILL WORK. You just need live tissue to make a "stem butt slurry". If it didn't work, like loc said, I wouldn't be able to clone live tissue from up the stem by the cap (where I usually take my biopsy from).
So get to it
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: How can I start a non-sterile culture without stem-butts? [Re: dankington]
#23787999 - 10/31/16 09:49 AM (7 years, 2 months ago) |
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Sounds like you're clueless op. 
N9ne hit it on the head
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Sirtalis


Registered: 10/05/16
Posts: 409
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Re: How can I start a non-sterile culture without stem-butts? [Re: dankington]
#23788005 - 10/31/16 09:53 AM (7 years, 2 months ago) |
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I started my backyard grow with whole mushrooms like the ones in your photos. No need for stem butts. If you can sacrifice one or two, blend it up in a blender and pour it into a container with sawdust and scotts earthgro woodchips. The mycelium will colonize the container.
All you have to do is soak the sawdust/woodchips over night. They key is to have a mix of very small pieces (sawdust) along with bigger chunks (woodchips). Drain it out and then pour your blend on it. Mix it up and cover the bin with soaked cardboard to prevent moisture from escaping. Make sure this bin has holes in it and is outside. The mycelium needs fresh air in order to fend off bacteria and other molds.
If you don't feel like taking the stem to agar, this is your best bet. To increase your odds of colonization use a few mushrooms. The more the better. If you want to guarantee success you'll need to go agar --> grains --> woodchips in sterile conditions. BUT plenty of people have had success throwing ENTIRE mushrooms into piles of woodchips. Including myself. Look at how the mycelium grows off the stem onto the woodchips in this photo.
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DavidReishi
Mediocrity Extraordinaire


Registered: 10/07/15
Posts: 1,333
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Re: How can I start a non-sterile culture without stem-butts? [Re: Sirtalis]
#23788012 - 10/31/16 09:57 AM (7 years, 2 months ago) |
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Thanks, Sirtalis! Help without the nastiness!
-------------------- Species found in the Bay area: P. allenii, P. cyanescens, P. ovoideocystidiata, P. stuntzii, P. azurescens
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DavidReishi
Mediocrity Extraordinaire


Registered: 10/07/15
Posts: 1,333
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Re: How can I start a non-sterile culture without stem-butts? [Re: dankington]
#23788030 - 10/31/16 10:03 AM (7 years, 2 months ago) |
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Quote:
dankington said: don't get your knickers in a twist, we're trying to help anyone that knows anything about mushrooms knows not to store them in plastic in the fridge
I don't want help from anyone that has to accompany it with nastiness. And about storing fresh Psilocybes, obviously my photo alone disproves "anyone who knows anything about mushrooms."
-------------------- Species found in the Bay area: P. allenii, P. cyanescens, P. ovoideocystidiata, P. stuntzii, P. azurescens
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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Re: How can I start a non-sterile culture without stem-butts? [Re: DavidReishi]
#23788040 - 10/31/16 10:06 AM (7 years, 2 months ago) |
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That's funny. So you thought we were being nasty, but in reality you were being needlessly stubborn about things that you know nothing about and other things that didn't matter. I wonder why you opted out of ratings…?

edit: ahh, mediocrity extraordinaire? Sounds accurate. Set the bar high, friend.
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DavidReishi
Mediocrity Extraordinaire


Registered: 10/07/15
Posts: 1,333
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Re: How can I start a non-sterile culture without stem-butts? [Re: dankington]
#23788059 - 10/31/16 10:10 AM (7 years, 2 months ago) |
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You do realize that we have an 18 and older policy here, don't you?
-------------------- Species found in the Bay area: P. allenii, P. cyanescens, P. ovoideocystidiata, P. stuntzii, P. azurescens
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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Re: How can I start a non-sterile culture without stem-butts? [Re: DavidReishi]
#23788067 - 10/31/16 10:13 AM (7 years, 2 months ago) |
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really? I could probably be your father, broseph.
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: How can I start a non-sterile culture without stem-butts? [Re: dankington]
#23788069 - 10/31/16 10:13 AM (7 years, 2 months ago) |
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of course you dont store fresh mushrooms in plastic bags, come on. everyone who's done it knows why not. they wont be fine for much longer than a cpl days.
and to everyone that said any part of the mushroom works for cloning.
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Sirtalis


Registered: 10/05/16
Posts: 409
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Re: How can I start a non-sterile culture without stem-butts? [Re: DavidReishi]
#23788070 - 10/31/16 10:14 AM (7 years, 2 months ago) |
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
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Re: How can I start a non-sterile culture without stem-butts? [Re: Sirtalis]
#23788185 - 10/31/16 10:54 AM (7 years, 2 months ago) |
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Why are we even trying to help this guy anymore? He clearly has all the answers.
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