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InvisibleDavidReishi
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How can I start a non-sterile culture without stem-butts?
    #23787075 - 10/30/16 10:07 PM (7 years, 2 months ago)

I have a plastic bag in the fridge with some Azures cut less than 36 hours ago. I didn't bring home any stem butts. Is there anything simple I can do to get some non-sterile cultures going?


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Species found in the Bay area: 
P. allenii, P. cyanescens, P. ovoideocystidiata, P. stuntzii, P. azurescens


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OfflineHuskies
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Re: How can I start a non-sterile culture without stem-butts? [Re: DavidReishi]
    #23787119 - 10/30/16 10:26 PM (7 years, 2 months ago)

You can in theory use any part of the mushroom. Try a sample from the stem.


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I call them Huskies cause you tell them to go "Mush! Mush""


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Offlinedextr0
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Re: How can I start a non-sterile culture without stem-butts? [Re: DavidReishi]
    #23787123 - 10/30/16 10:28 PM (7 years, 2 months ago)

Clone to agar.


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OfflinePsilosopherr
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Re: How can I start a non-sterile culture without stem-butts? [Re: dextr0]
    #23787154 - 10/30/16 10:49 PM (7 years, 2 months ago)

spores baby. :cool2:

Would need cleaning up on agar to get a clean culture.


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Invisibledankington
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Re: How can I start a non-sterile culture without stem-butts? [Re: Psilosopherr]
    #23787159 - 10/30/16 10:52 PM (7 years, 2 months ago)

He can likely take a few stem butts, blend them up with water and use the slurry on wood chips, actually.


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OfflinePsilosopherr
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Re: How can I start a non-sterile culture without stem-butts? [Re: dankington]
    #23787166 - 10/30/16 10:55 PM (7 years, 2 months ago)

whole point of the thread is to not use stem buts, actually :wink: :grin:


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Invisibledankington
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Re: How can I start a non-sterile culture without stem-butts? [Re: Psilosopherr]
    #23787183 - 10/30/16 11:05 PM (7 years, 2 months ago)

and yet, he has a bag full of azures in his fridge :shrug: kinda silly


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OfflineMushierage
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Re: How can I start a non-sterile culture without stem-butts? [Re: dankington]
    #23787198 - 10/30/16 11:16 PM (7 years, 2 months ago)

Have any extra pins?  Just use the entire thing.  Boom.


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Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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Invisibledankington
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Re: How can I start a non-sterile culture without stem-butts? [Re: Mushierage]
    #23787203 - 10/30/16 11:19 PM (7 years, 2 months ago)

well, but how does he have a bag of azzies and no stem to make a slurry from? I think OP was simply too literal with the term "stem butts". Also, a plastic bag in the fridge? You should have used a paper bag, so they're probably kinda gross by now.


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Invisiblefreespeech
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Re: How can I start a non-sterile culture without stem-butts? [Re: dankington]
    #23787231 - 10/30/16 11:35 PM (7 years, 2 months ago)

I've prepared a gallon bucket of soaked woodchips (with a tiny bit of bleach included in the soak) and inoculated it with cyanescens mycelium chips that I dug from a local outdoor patch. This was successful; the entire bucket colonized beautifully. You could try something similar with some of the stems from your haul, but I doubt you're going to get anything good going with nasty wet stems that have spent a day and a half in your fridge in a plastic bag.


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InvisibleDavidReishi
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Re: How can I start a non-sterile culture without stem-butts? [Re: freespeech]
    #23787294 - 10/31/16 12:05 AM (7 years, 2 months ago)

Thanks, guys. Btw, inside a plastic bag in the fridge is how you keep fresh mushrooms fresh. A paper bag would never work...the mushies would dry out.


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Species found in the Bay area: 
P. allenii, P. cyanescens, P. ovoideocystidiata, P. stuntzii, P. azurescens


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Invisiblefreespeech
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Re: How can I start a non-sterile culture without stem-butts? [Re: DavidReishi]
    #23787302 - 10/31/16 12:07 AM (7 years, 2 months ago)

Kinda depends on how wet they are when you put them in there. Fresh mushrooms picked when it's wet outside tend to get slimy and gross in a closed plastic bag in the fridge. I'd rather they dry out a little bit than get slimy. It's not like they're not going to dehydrate in a paper bag in the fridge. Fresh air is a friend to mushrooms.


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InvisibleDavidReishi
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Re: How can I start a non-sterile culture without stem-butts? [Re: freespeech]
    #23787310 - 10/31/16 12:11 AM (7 years, 2 months ago)

Quote:

freespeech said:
It's not like they're not going to dehydrate in a paper bag in the fridge.




Actually they do, but that's besides the point.


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Species found in the Bay area: 
P. allenii, P. cyanescens, P. ovoideocystidiata, P. stuntzii, P. azurescens


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Invisiblefreespeech
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Re: How can I start a non-sterile culture without stem-butts? [Re: DavidReishi]
    #23787314 - 10/31/16 12:13 AM (7 years, 2 months ago)

Right, if you want to get technical about it, sure. I said they will dry out a little bit, but by "dehydrate," I mean they won't get close to cracker dry any time soon. I'm guessing they would retain most of their moisture for several days at least.


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InvisibleDavidReishi
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Re: How can I start a non-sterile culture without stem-butts? [Re: freespeech]
    #23787316 - 10/31/16 12:15 AM (7 years, 2 months ago)

True.


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Species found in the Bay area: 
P. allenii, P. cyanescens, P. ovoideocystidiata, P. stuntzii, P. azurescens


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InvisibleLocN9ne
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Re: How can I start a non-sterile culture without stem-butts? [Re: DavidReishi]
    #23787346 - 10/31/16 12:40 AM (7 years, 2 months ago)

They are going to get nasty quick... A paper or cloth bag or just leave them sitting out... But never a plastic bag... Thats bad form... And idk what you think a stem butt is, but you can use the whole mushroom... Pick a part and call it a stem butt... If you want to, throw a piece of the stem in some hydrated cardboard let it grow a bit then use that to noc up some chips...

Ol' boy with the bleach in his bucket, the bleach didnt do anything fyi.


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InvisibleDavidReishi
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Re: How can I start a non-sterile culture without stem-butts? [Re: LocN9ne]
    #23787374 - 10/31/16 01:01 AM (7 years, 2 months ago)

Quote:

LocN9ne said:
They are going to get nasty quick... A paper or cloth bag or just leave them sitting out... But never a plastic bag... Thats bad form...




Nonsense when it comes to the fridge...but ok, thanks.


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Species found in the Bay area: 
P. allenii, P. cyanescens, P. ovoideocystidiata, P. stuntzii, P. azurescens


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Offlineoakley
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Re: How can I start a non-sterile culture without stem-butts? [Re: DavidReishi]
    #23787388 - 10/31/16 01:10 AM (7 years, 2 months ago)

From stamets mushroom cultivator

The cap, the upper region of the stem and/or the
area where the gill plate joins the underside of the cap are the best locations for excising clean tissue.
Some mushrooms have a thick cuticle overlaying the cap. This skin can be peeled back and a tissue
culture can be taken from the flesh underlying it. Wipe the surface of the mushroom with a cotton
swab soaked in alcohol and remove any dirt or damaged external tissue. Break the mushroom cap
or stem, exposing the interior hyphae. Immediately flame a scalpel until red-hot and cool in a media
filled petri dish. Now cut into the flesh removing a small fragment of tissue. Transfer the tissue fragment to the center of the nutrient filled petri dish as quickly as possible, exposing the tissue and agar
to the open air for a minimal time. Repeat this technique into at least three, preferably five more
dishes. Label each dish with the species, date, type of culture (tissue) and kind of agar medium. If
successful, mycelial growth will be evident in three to seven days.

Also I can say from experience that any kind of mushroom stored in a plastic bag in the fridge will get moldy pretty soon.


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OfflineWhyDidiDoThis
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Re: How can I start a non-sterile culture without stem-butts? [Re: DavidReishi]
    #23787417 - 10/31/16 01:33 AM (7 years, 2 months ago)

Quote:

DavidReishi said:
Thanks, guys. Btw, inside a plastic bag in the fridge is how you keep fresh mushrooms fresh. A paper bag would never work...the mushies would dry out.




At work in the kitchen we must not wrap or seal the fresh culinary mushrooms.
In the walk-in fridge.
Keep the bag open to breathe or face rotting.


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Offlinemolemole
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Re: How can I start a non-sterile culture without stem-butts? [Re: WhyDidiDoThis]
    #23787468 - 10/31/16 02:40 AM (7 years, 2 months ago)

Couple weeks ago, i printed some azures on tinfoil. I ussually print in a Tupperware and mist the sides and lid. I left these caps to print for 2 days. When i pulled the caps all 3 where growing myc right where the stem was cut at. I put these to unsterized, unpasturized soaked wood chips in a gallon zip loc. Leave top cracked open a 1/4 to 1/2 way. Poke some hole in the bottom for drainage. Stems will just rot in the chips unless they have the stem butt with them.


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Edited by molemole (10/31/16 02:41 AM)


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