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OfflineWalleye
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Registered: 10/30/16
Posts: 5
Last seen: 7 years, 18 days
Isolation of Mycelium
    #23786499 - 10/30/16 06:25 PM (7 years, 2 months ago)

I initially tried to clone a commercial strain of oyster mushroom on agar. The first results showed good signs but than trichoderm started to grow along with cobweb mold. I again tried to transfer to agar and finally though I had it.

I transfered to grain and watched the mycelium grow but when I shook grain spawn to transfer to 5 lb bag there was black specs all over, growing in the core of the spawn. I transfered it anyways to see what happened.

Little black pins started forming, there was bacteria in the bag I could see contamination everywhere but the mycelium was growing faster than contamination could keep up.

I decided to cut bag right at the leading front of growth and extract the mycelium. I used sterile techniques and transfered those grains to agar. Seemed like mycelium took over the plate completely, transfered to spawn and it looks like no contam along hyphae. Looks contam free.

Will keep you posted


Anyone else ever have a similar experience or isolated the mycelium through a grow bag rather than trying to fight with it on agar?

Anyone have any unique isolation methods they figure work best?

Cheers


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