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OfflinePlazmotech
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Registered: 10/11/16
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Last seen: 3 years, 1 month
Spore print got alcohol on it
    #23785847 - 10/30/16 03:14 PM (8 years, 2 months ago)

I innoc'd a couple of agar plates yesterday with a spore print I bought. I proceeded to wipe down the inside of a small bag with alcohol then I tried to dry it and put the spore print in there (folded up tin foil). Turns out the bag wasn't completely dry, and I got some alcohol overnight on the print, which turned it kind of gooey/wet. I proceeded to make a spore syringe with that. Do you think the spores will still germinate?


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When a man lies, he murders some part of the world.
These are the pale deaths which men miscall there lives.
All this I cannot bear to witness any longer.
Cannot the kingdom of salvation take me home?
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OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 4 months, 23 days
Re: Spore print got alcohol on it [Re: Plazmotech]
    #23785940 - 10/30/16 03:37 PM (8 years, 2 months ago)

I'd say so, spores are pretty resilient, they can withstand a little alcohol

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OfflineWalleye
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Re: Spore print got alcohol on it [Re: Plazmotech]
    #23786555 - 10/30/16 06:46 PM (8 years, 2 months ago)

I would say it depends on exposure time. The Alcohol would evaporate fast and spores can withstand some pretty extreme conditions. Now if that was mycelium could but a different story. Its probably ok, best thing to do is try it and see for yourself.

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OfflinePlazmotech
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Registered: 10/11/16
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Re: Spore print got alcohol on it [Re: Walleye]
    #23787063 - 10/30/16 10:01 PM (8 years, 2 months ago)

Quote:

Psilosoulful said:
I'd say so, spores are pretty resilient, they can withstand a little alcohol




Yes!!!! Please PLEASE work

Quote:

Walleye said:
I would say it depends on exposure time. The Alcohol would evaporate fast and spores can withstand some pretty extreme conditions. Now if that was mycelium could but a different story. Its probably ok, best thing to do is try it and see for yourself.




It was exposed almost 24 hours, I'd say…


--------------------
When a man lies, he murders some part of the world.
These are the pale deaths which men miscall there lives.
All this I cannot bear to witness any longer.
Cannot the kingdom of salvation take me home?
Plazmotech's Kratom Grow

Extras: Filter Print Post Top
OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 4 months, 23 days
Re: Spore print got alcohol on it [Re: Plazmotech]
    #23787305 - 10/31/16 12:09 AM (8 years, 2 months ago)

I wouldn't worry too much about it, try it out and let us know how it goes :goodluck:

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OfflinePlazmotech
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Registered: 10/11/16
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Last seen: 3 years, 1 month
Re: Spore print got alcohol on it [Re: Psilosoulful]
    #23852386 - 11/20/16 06:31 PM (8 years, 1 month ago)

Holy fuck boys! That shit didn't grow forever, after a literal month I took a look at it and there was about a quarter sized growth! I'm going to do a transfer tomorrow. The alcohol also seemed to destroy all the mold and bacteria, as the other two plates (pre-alcohol) got a fuck load of contams, but these didn't!

Fuck!


--------------------
When a man lies, he murders some part of the world.
These are the pale deaths which men miscall there lives.
All this I cannot bear to witness any longer.
Cannot the kingdom of salvation take me home?
Plazmotech's Kratom Grow

Extras: Filter Print Post Top
Invisibleazur
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Registered: 04/21/12
Posts: 28,103
Loc: Daid
Re: Spore print got alcohol on it [Re: Plazmotech]
    #23852404 - 11/20/16 06:38 PM (8 years, 1 month ago)

Hmmmm :takingnotes:


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FOTTSE!!!
ALL NOOBS READ THIS!!!


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