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OfflineShroomoroto42
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Registered: 10/30/16
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Last seen: 7 years, 2 months
Glovebox sterilization and usage for fruiting chamber?
    #23785568 - 10/30/16 01:54 PM (7 years, 2 months ago)

Hello everyone, I'm just starting my first project using PF Tek. I read a lot on Shroomery and also watched some videos from around the web. I'm very excited to start my first grow and I'm grateful I found this amazing place!

I decided to make a glovebox. I will make my own syringes from spore print because prints get delivered more easily than syringes.

It will be a still air box from a plastic container with hand holes and gloves attached to them. What I plan to do is the following:
1. Fill syringe with sterilized water
2, Put it in the box together with everything needed
3. Close the box and carefully wipe the inside of the box, gloves and objects with Ethyl Alcohol 70%
4. Wait about 30 minutes and start the print to syringe transfer process
5. I'll not use flame sterilization inside the box as I don't want to fire it

Now... the questions:
1. Will the alcohol evaporate when the box is closed or should I let some air exchange by opening the lit a bit?
2. Is it needed/crucial to spray the air in the box with Ethyl Alcohol 70%?
3. Is it too bad I'll not use fire sterilization? I may still use fire on the syringe needle before inserting it in the box.
4. Is it possible to use the same box, used as glovebox to inoculate in it? Should it be complete closed from air exchange when inoculating? I know jar holes must be left open.
5. Can I use the same box for fruiting? Some guides say to make a lot of holes on the box and other say to just close the box (without any holes) and open once a day for air exchange. Which of these techniques is better? I would prefer the one without the holes if it's ok to use it.
6. It's been said on the guides to put the jars for inoculation and check them a week later. Is it important to not check them earlier?

Thanks in advance :smile:)


Edited by Shroomoroto42 (10/30/16 02:43 PM)


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InvisibleInocuole
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Re: Glovebox sterilization and usage for fruiting chamber? [Re: Shroomoroto42]
    #23785600 - 10/30/16 02:03 PM (7 years, 2 months ago)



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OfflinePsilosopherr
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Re: Glovebox sterilization and usage for fruiting chamber? [Re: Shroomoroto42]
    #23785627 - 10/30/16 02:14 PM (7 years, 2 months ago)

I and most others on here will recommend against attaching the gloves to your SAB.

how to make spore syringes from prints

1) Soapy water won't evaporate, wipe with iso alcohol and then soapy water if it makes you feel better, I like to do it sometimes but soapy water man.

2) No, its recommended for wiping down the jars, tools, and your hands/arms though. Idk what ethyl alcohol is, I'm assuming its the same as isopropyl alcohol?

2a) This is part of why you don't want attached gloves, flame sterilization is important, just do it outside of your box, moving carefully to not stir up more air than necessary

3) Yes, I and many others use the same still air box for all clean work. It doesn't need to be totally closed, in fact I heard of one guy testing the air flow by burning incense inside his SAB. He found that sizable (6 inches I think? not super huge) arm holes reduced airflow inside the box.

4) Check out my signature, the tek compendium, read up on monotubs and shotgun FC's. I wouldn't use my SAB box for fruiting, no way, it'll be full of spores and substrate, which would just add contaminants to your SAB.

5)It doesn't matter, its just trying to prevent you from checking it constantly, which also doesn't matter but is pointless.

some links you'll find useful
https://www.shroomery.org/forums/showflat.php/Number/21889950#21889950
https://www.shroomery.org/forums/showflat.php/Number/17441645#17441645 (written for G2G but this is how I prep my box for most everything)
https://www.shroomery.org/forums/showflat.php/Number/20048771#20048771

and again check out my sig, its full of links you'll find helpful


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InvisibleInocuole
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Re: Glovebox sterilization and usage for fruiting chamber? [Re: Psilosopherr]
    #23785641 - 10/30/16 02:17 PM (7 years, 2 months ago)

Quote:

rbalzer said:
Idk what ethyl alcohol is, I'm assuming its the same as isopropyl alcohol?




Hint: you're not allowed to drive after consuming a certain amount of it


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OfflinePsilosopherr
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Re: Glovebox sterilization and usage for fruiting chamber? [Re: Inocuole]
    #23785647 - 10/30/16 02:19 PM (7 years, 2 months ago)

damnit, I should've known that :doh:

yeah OP, high proof ethyl alcohol is expensive, use rubbing (iso) alcohol. 70 or 90 percent, 70 is better because it evaporates a little slower.


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OfflineNDStepp84
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Re: Glovebox sterilization and usage for fruiting chamber? [Re: Shroomoroto42]
    #23785748 - 10/30/16 02:48 PM (7 years, 2 months ago)

Spray the inside of a SAB with soapy water, not alcohol. Alcohol evaporates fast, and it is also dangerous to have a flame next to a chamber full of volatile fumes. You can't sterilize a SAB, nor do you need to. You don't need sterile air you need still air,  anything floating around sticks to the walls.
Quote:

Inocuole said:
http://www.shroomery.org/forums/showflat.php/Number/20048771#20048771



^


--------------------

"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


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OfflineShroomoroto42
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Registered: 10/30/16
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Re: Glovebox sterilization and usage for fruiting chamber? [Re: Psilosopherr]
    #23785779 - 10/30/16 02:57 PM (7 years, 2 months ago)

Quote:

rbalzer said:
Idk what ethyl alcohol is, I'm assuming its the same as isopropyl alcohol?




I had a hard time finding Isopropyl alcohol in my country and that's why I wanted to get Ethyl alcohol. It turned out I searched it wrong. I just found out it's called Isopropanol here, so I'll get some soon :smile:. I don't know what's the difference.

As for the fruiting, I'm not sure of some things, but for now I'll concentrate on the making syringe, making box and inoculation part.

The link you gave me for the strynge making tek is exactly the one I intend to use. There is also a popular video on youtube showing how it's done.

I'll check the links you and Inocuole gave me, do some more reading and ask again if I have new questions. Thanks :smile:


Edited by Shroomoroto42 (10/30/16 02:58 PM)


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InvisibleInocuole
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Re: Glovebox sterilization and usage for fruiting chamber? [Re: Shroomoroto42]
    #23785894 - 10/30/16 03:26 PM (7 years, 2 months ago)

Isopropanol is to Isopropyl Alcohol as Ethanol is to Ethyl Alcohol.  Same things, latter are the more commonly used terms.  Both are correct but the former terms are more specific.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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Jokes are funny until they're about you


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Offlinespongegar
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I'm a teapot


Registered: 09/20/16
Posts: 71
Last seen: 3 years, 1 month
Re: Glovebox sterilization and usage for fruiting chamber? [Re: Shroomoroto42]
    #23785981 - 10/30/16 03:47 PM (7 years, 2 months ago)

Check out surface desinfectants. The one I use is just 70% isopropanol, have difficulities finding isopropyl alcohol here too.


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Offlineomnifarious
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Re: Glovebox sterilization and usage for fruiting chamber? [Re: Inocuole]
    #23785991 - 10/30/16 03:49 PM (7 years, 2 months ago)

if youre in the US you can buy farm grain alcohol for absurdly cheap (~$2-3/l). Its undrinkable, and is 97% so you will need to dilute it. Obviously youre not in the us but I thought you might be able to find similar


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