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OfflineSpiggle
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Agar experiment
    #23784325 - 10/30/16 02:12 AM (7 years, 2 months ago)

Hey
I have a agar dish with some healthy looking mycelial growth, i did isolate the nice rizomorphic parts and now feel like doing a wee experiment with the rest of my dish... any ideas? i was thinking i could try fruit from the dish is this possible? any cool ideas?
Thanks
Spiggle



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Offlinefilyep
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Re: Agar experiment [Re: Spiggle]
    #23784341 - 10/30/16 02:39 AM (7 years, 2 months ago)

So your going to wee on it and see if it fruits?


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InvisibleJosex
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Re: Agar experiment [Re: Spiggle]
    #23784342 - 10/30/16 02:41 AM (7 years, 2 months ago)

Yes, you can let that plate grow out and wait to see if it pins invitro, although it's not guaranteed that it will. If it does, you can pluck out the nicest pin from a cluster (if possible) using sterile tweezers and following proper sterile tech, then transfer the pin to a new receiving plate.

This pin should be very clean cos it was grown invitro and will probably not need cleaning up, although further transfers would not do any harm just to be safe.

This clone will guarantee that you have a fruiter, but you have to test it out to see if it performs well and it's worth keeping.

If the agar pool in that ms plate is shallow and/or the genetics you got in there need to take their sweet time to pin, it's possible that the plate might not have enough water content to allow a pinset.

Since you can't know if the plate is going to pin, you could make an LI with that plate and inoculate some PF tek style invitro jars and this will allow you to clone invitro pins as well. Or you could just use that plate to noc some grain jars to do a regular grow, although you can never be sure that plate is sufficiently clean to guarantee clean spawn because it came from MS.


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InvisibleInocuole
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Re: Agar experiment [Re: Josex]
    #23784529 - 10/30/16 06:45 AM (7 years, 2 months ago)

That doesn't really look all that healthy.  Might not be worth trying to fruit.


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InvisibleMunchauzen
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Re: Agar experiment [Re: Inocuole] * 1
    #23784547 - 10/30/16 07:02 AM (7 years, 2 months ago)

:whathesaid:

only "experiment" I would do is inoculate a trash can with that and put it by the curb.


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OfflineSpiggle
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Re: Agar experiment [Re: Munchauzen]
    #23785071 - 10/30/16 11:16 AM (7 years, 2 months ago)

Wow its pretty bad then! I went ahead and isolated the strain again maybe after a few isolations it will be worthy of grains and not the trash can! Still a big noobie this is my first attempt cheers for the tips


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InvisiblebodhisattaMDiscordReddit
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Re: Agar experiment [Re: Spiggle]
    #23785094 - 10/30/16 11:23 AM (7 years, 2 months ago)

You took transfers not isolated a strain.


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InvisibleJosex
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Re: Agar experiment [Re: Spiggle]
    #23785159 - 10/30/16 11:44 AM (7 years, 2 months ago)

Quote:

Spiggle said:
Wow its pretty bad then! I went ahead and isolated the strain again maybe after a few isolations it will be worthy of grains and not the trash can! Still a big noobie this is my first attempt cheers for the tips




Try prints to agar next time. In the pic it looks like you squirted an awful lot of spore solution.


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OfflineSpiggle
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Re: Agar experiment [Re: Josex]
    #23785629 - 10/30/16 02:15 PM (7 years, 2 months ago)

Hey josex,
Yeah i did use a spore syringe how could you tell?


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InvisibleSubnet Mask
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Re: Agar experiment [Re: Spiggle]
    #23785741 - 10/30/16 02:46 PM (7 years, 2 months ago)

Because it looks like a giant blob instead of growing out from a central point.


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Offlineomnifarious
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Re: Agar experiment [Re: Subnet Mask]
    #23786023 - 10/30/16 03:58 PM (7 years, 2 months ago)

fruiting from the dish is not practical but ive seen pictures of people casing then fruiting from them


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Offlinespore-ty
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Re: Agar experiment [Re: omnifarious]
    #23786085 - 10/30/16 04:13 PM (7 years, 2 months ago)

If your gonna use a syringe literally all that is needed is a single drop


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