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Hallucyjunkie99

Registered: 10/21/15
Posts: 132
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How does my jar look
#23783992 - 10/29/16 09:54 PM (7 years, 3 months ago) |
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Here is my first wbs jar 4 days in.I started another one last night !
Edited by Hallucyjunkie99 (10/29/16 09:57 PM)
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Thedillestpickle
cultured



Registered: 02/02/16
Posts: 1,170
Loc: Canada
Last seen: 3 years, 7 months
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Looks good to me so far. How did you inoculate?
I like that technique with the pp5's, I just crack the lid open about an inch turn to the left and it gets plenty of gas exchange.
Are you prepping one jar at a time?
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Hallucyjunkie99

Registered: 10/21/15
Posts: 132
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I inoculated with agar and no I'm not doing one jar at a time I'm only using 2 quarts for a mini mono and I didnt have the other wbs jar prepped(wasn't home ) but last night I ticitac toe tiger dropped into another one
I'm isolating a strain on pasty plates right now then doing a 66qt mono but for now I decided to see if I could produce a good fruit to clone
Edited by Hallucyjunkie99 (10/29/16 10:07 PM)
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Thedillestpickle
cultured



Registered: 02/02/16
Posts: 1,170
Loc: Canada
Last seen: 3 years, 7 months
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nice. Sounds like you've done things right
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Hallucyjunkie99

Registered: 10/21/15
Posts: 132
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I did do ms - agar - grain I had no visible contams does it look like it has bacterial contams or not ? Just wanna make sure this will be OK
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Raven44
Entry not permitted to muggles



Registered: 12/07/13
Posts: 1,970
Loc: My sovereign reality bubble
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It's. Little early to tell
I see some condensation tho
Try to keep temperature fluctuations to a minimal in the spawn colonization area. Wherever it may be.
Large temp swing leads to lots of condensation which can cause more problems
U will prob be fine tho. Look like those will be done in a shake or two
Once fully colonized due the shake test. I love it.
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Hallucyjunkie99

Registered: 10/21/15
Posts: 132
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Re: How does my jar look [Re: Raven44]
#23784117 - 10/29/16 10:57 PM (7 years, 3 months ago) |
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I have shaken it before this photo should I shake again or wait until 100%
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Thedillestpickle
cultured



Registered: 02/02/16
Posts: 1,170
Loc: Canada
Last seen: 3 years, 7 months
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Hard to tell just yet. Usually you can tell if there is bacteria when the jar is almost fully colonized and some areas won't colonize. Those areas will then turn all gooey and wet and will never colonize.
Depending on how badly bacterial it is you can sometimes get away with spawning, however spawning with bacteria is more of a pain in the ass with a good chance of getting trich before a first flush.
Personally I shy away from tiger drop. In my experience bacterial contamination on agar often starts at the edges, so I tend to think bacteria are probably hanging out along the rim and sides of a lot of clean looking plates and just haven't had the luck of finding the agar yet. Basically I consider the sides of plates a contamination vector. Tiger drop involves a lot of potential for agar to touch the sides of the plate which makes it more likely to pick up stray bacteria... maybe not enough to cause a problem. Either way It's not my preferred method. A single wedge can also do the same job and not have that vector.
Another main source of bacteria is undercooked grain. 1.5 hours at full pressure for those pp5's should suffice, but longer won't hurt.
I would wait until full colonization with those. No shakes needed. Once it's ready just pop them out into the bulk sub and break it up with your hands
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Hallucyjunkie99

Registered: 10/21/15
Posts: 132
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For the jar in the picture I picked agar from the center and avoided edges actually my second jar that that is a different story
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Raven44
Entry not permitted to muggles



Registered: 12/07/13
Posts: 1,970
Loc: My sovereign reality bubble
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When to shake is a personal preference. We cannot answer that w out pics or great description with reference to the amount of colonization in last pic...
Ime it's not always obvious if bacteria lies hidden.
If metabolites form, sure sign NOT to expand the jar into more JARS.
U may be able to get away w spawning it tho to bulk.
The shake test is used to ensure a jar is worthy of making more JARS for expansion. Once fully colonized wait few days to ensure full colonization.
Shake the jar. Wait 24 hrs. No longer than 48 hrs. Then check for signs of wetness and metabolites. U should see all the kernal fuzzing back over with white growth and zero wetness.
Any wet jars should be set aside and if they do not fully recolonize I never use those for anything personally maybe outdoors.
U can also after 24-48 hrs tip the jar upside down to check thay the kernal are held together again by the new fuzzy growth.
It won't look fully colonized like before but u will know when one jar is wet and bad then u will always know I swear by it
Edited by Raven44 (10/29/16 11:17 PM)
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Hallucyjunkie99

Registered: 10/21/15
Posts: 132
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Re: How does my jar look [Re: Raven44]
#23784147 - 10/29/16 11:21 PM (7 years, 3 months ago) |
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Awesome I heard smell is another way to tell but thanks everyone for the info will be sure to remember this
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Thedillestpickle
cultured



Registered: 02/02/16
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I have a hard time being able to tell using smell. I can open a jar that is clearly bacterial and it smells fine to me. By the time you would really smell it I would think it would look rather obvious that it was bad.
visually though you can tell a lot by what has been mentioned. Metabolites, or wet grains, or even slightly damper looking grains that don't fully colonize over.
Also when you achieve very clean spawn you'll notice that the mycelium do not grow as white and thick, but rather lightly cover all the grains. This is actually the best indicator to look for because it is how you can tell very slightly and mostly hidden bacterial from totally clean nearly bacteria free spawn. Hidden bacteria that isn't causing metabolites or wet spots will often still be identifiable because the mycelium will grow more densely. That can take a bit time to learn to see, and it can also depend on how you inoculate and how that particular culture grows out on grain.
Here's what I consider very clean spawn. This is probably the cleanest I've grown so far.
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blindingleaf
blue collar underworld


Registered: 07/19/13
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Quote:
Thedillestpickle said: I have a hard time being able to tell using smell. I can open a jar that is clearly bacterial and it smells fine to me. By the time you would really smell it I would think it would look rather obvious that it was bad.
visually though you can tell a lot by what has been mentioned. Metabolites, or wet grains, or even slightly damper looking grains that don't fully colonize over.
Also when you achieve very clean spawn you'll notice that the mycelium do not grow as white and thick, but rather lightly cover all the grains. This is actually the best indicator to look for because it is how you can tell very slightly and mostly hidden bacterial from totally clean nearly bacteria free spawn. Hidden bacteria that isn't causing metabolites or wet spots will often still be identifiable because the mycelium will grow more densely. That can take a bit time to learn to see, and it can also depend on how you inoculate and how that particular culture grows out on grain.
Here's what I consider very clean spawn. This is probably the cleanest I've grown so far.

 
great explanation and example +5
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Kenetic
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-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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