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jellyfish


Registered: 10/02/05
Posts: 7,457
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Mexicana, tampanensis, galindoi and cubensis grow
#23783443 - 10/29/16 06:28 PM (7 years, 3 months ago) |
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So I've never posted in this forum. Always with the begineers because I really sucked when I was younger. Fruited pf cakes in a fishtank with perlite at the bottom and inoculated everything in my kitchen with the oven hood on. But a few years ago I got into grain and then built a flow hood and did my first monotubs. I made some nice cultures of some nice cube fruits but I've been out of the hobby for 3 years, my cultures all dried up and my flowhood just collected dust (not really, had that shit all wrapped up).

I just inoculated 4 jars each of malt extract and agar with spore prints of ps.tampanensis, ps. galindoi, ps. mexicana and a spore print of my nicest mushroom from my monotub 3 years ago. It's been 3 days and no sign of growth or contam. I opened one of the jars up for a few minutes in front of the floor hood in my dusty ass basement as a test and it hasn't shown any signs of contam either. My basement does get pretty cold though, temps fluctuate sometimes the rads turn on and it's really warm. I've got them just on a shelf right now.
From what I read I can incubate all 4 species at the same temp (86F/30C) so all I need is a thermometer and I have a fishtank heater and tubs from back when I grew. When I inoculated I flamed the innoc loop and cooled it for a second in the breeze of the flow hood and then dabbed it first on the agar to cool it off further. I'm wondering if could have still been too hot and fried the spores. The newest spore prints were 1 years old, the oldest 3 years ago. I'm reading different things about how long they last, they were in a cool dark place for almost the entire time, except a couple days where my place was unusually warm. Any advice would be appreciated also I haven't done any mycology in years let me know if this is the wrong forum.
Edit: I'm seeing a couple spot on one of my tamp and one of my mexicana plates. Not sure how big to let them grow before I make a new agar batch and prepare todo some transfers. I'll try to get pics tomorrow. So far, they seem pretty bright white which worries me because I thought those strains were less snow-white than cubensis.
Edited by jellyfish (10/30/16 12:02 AM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Mexicana, tampanensis, galindoi and cubensis grow [Re: jellyfish]
#23785036 - 10/30/16 10:59 AM (7 years, 2 months ago) |
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Yes this belongs in regular mushroom cultivation. And incubation at 86 might be fine for everything but at least for cubes its quite dated info. Room temperature is just fine for cubes and the other species too. 86F is more favorable for contamination
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jellyfish


Registered: 10/02/05
Posts: 7,457
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Re: Mexicana, tampanensis, galindoi and cubensis grow [Re: jellyfish]
#23785675 - 10/30/16 02:25 PM (7 years, 2 months ago) |
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Yea but if you want faster growth and your cultures are clean I see no reason not to speed them up a bit.
Anyways I felt this was advanced because it involved cultures and multiple species. Hopefully mods move this and let me off with a warning. The white spot I'm seeing on my plates, I dunno, seems a bit too white. But not big enough for me to feel like it's time to make a new batch of agar and start doing transfers.
edit: quick question, I remember when I first joined this site we would keep our cultures in the dark to prevent premature pinning. Then RR said he keeps his on a shelf and that light doesn't really matter. Does this apply to sclerotia forming strains too? Should the cultures be in the dark or is on a shelf fine?
Mods plz move this thread to where it is more appropriate, sorry.
Edited by jellyfish (10/30/16 02:48 PM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Mexicana, tampanensis, galindoi and cubensis grow [Re: jellyfish]
#23785832 - 10/30/16 03:09 PM (7 years, 2 months ago) |
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Generally light is a secondary pinning trigger in Psilocybes They form where conditions are best for fruiting light doesn't make them pin on sides of containers.
Cultivation takes care of all psychedelic species. Unless you figure out azurecens indoors then that would go here first in the figure it out stages.
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jellyfish


Registered: 10/02/05
Posts: 7,457
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Re: Mexicana, tampanensis, galindoi and cubensis grow [Re: bodhisatta]
#23785936 - 10/30/16 03:36 PM (7 years, 2 months ago) |
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Thanks man. And yea, I'll delete this thread if mods don't move it. I wanted to make more of a grow log/journal type deal but I'm new with agar work and have a lot of questions.
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oakley
Stranger

Registered: 10/31/16
Posts: 55
Last seen: 2 years, 4 months
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Re: Mexicana, tampanensis, galindoi and cubensis grow [Re: jellyfish]
#23787454 - 10/31/16 02:17 AM (7 years, 2 months ago) |
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For sclerotia forming species 86 F might be too high. I remember seeing somewhere that 82F is the optimum mycelial growth temperature for those species. Stamets lists 75-81 F for colonization.
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